• 제목/요약/키워드: UV detection

검색결과 549건 처리시간 0.032초

고속액체크로마토그래피에 의한 밀전분 지방질에 함유된 리소레시친의 정량 (Quantitative Analysis of Lysophosphatidyl Choline (LPC) in Wheat Starch Lipids by High Performance Liquid Chromatography)

  • 신명곤;민봉기;장판식
    • 분석과학
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    • 제5권3호
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    • pp.339-343
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    • 1992
  • HPLC-UV방법을 이용하여 밀전분 지방질의 주성분인 LPC를 정량하기 위해 HRW 밀전분 지방질로부터 순수한 LPC를 분리하여 LPC의 표준곡선을 작성하였다. 밀 품종별 LPC에서 대한 HPLC peak responses는 시료간 큰 차이를 보여주지 않아 HRW 밀전분 지방질로부터 만든 LPC의 HPLC-UV 표준곡선은 밀전분 지방질에 함유되어 있는 LPC의 함량을 신속하고 정확하게 정량하는 데 이용할 수 있음을 보여 주었다.

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EHP (Enterocytozoon hepatopenaei)에 오염된 사육기구에 대한 UV-C와 차아염소산칼슘의 소독 효과 (The disinfection effect of UV-C and calcium hypochlorite to shrimp farm instruments contaminated with EHP (Enterocytozoon hepatopenaei))

  • 류지민;노을빛;김보성
    • 한국어병학회지
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    • 제37권1호
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    • pp.139-145
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    • 2024
  • In this study, nylon mash and silicone tube mainly used as shrimp farm equipment were contaminated with Enterocytozoon hepatopenaei (EHP) which is the cause of Hepatopancreatic microsporidiosis (HPM), and were treated with calcium hypochlorite or UV-C disinfection methods for EHP eradication. As a result, similar with the control group (not disinfected), EHP was detected on the nested PCR until the 10 days in the UV-C single treated group. On the other hand, EHP was not detected from 7 days in calcium hypochlorite single treated group (total concentration 200 ppm as available chlorine), and combination of calcium hypochlorite and UV-C treated group revealed no detection of EHP from 3 days. It is appropriate that treated with UV-C and calcium hypochlorite for 3 days or single treated with calcium hypochlorite for 7 days to eradicate EHP on contaminated instrument used in shrimp farms. In contrast, disinfection effect of only using UV-C is very low.

Mass Spectrometry-Based Analytical Methods of Amatoxins in Biological Fluids to Monitor Amatoxin-Induced Mushroom Poisoning

  • Choi, Jin-Sung;Lee, Hye Suk
    • Mass Spectrometry Letters
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    • 제13권4호
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    • pp.95-105
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    • 2022
  • Amatoxin-induced mushroom poisoning starts with nonspecific symptoms of toxicity but hepatic damage may follow, resulting in the rapid development of liver insufficiency and, ultimately, coma and death. Accurate detection of amatoxins, such as α-, β-, and γ-amanitin, within the first few hours after presentation is necessary to improve the therapeutic outcomes of patients. Therefore, analytical methods for the identification and quantification of α-, β-, and γ-amanitin in biological samples are necessary for clinical and forensic toxicology. This study presents a literature review of the analytical techniques available for amatoxin detection in biological matrices, and established an inventory of liquid chromatography (LC) techniques with mass spectrometry (MS), ultraviolet (UV) detection, and electrochemical detection (ECD). LC-MS methods using quadrupole tandem mass spectrometry, time-of-flight mass spectrometry, and orbitrap MS are powerful analytical techniques for the identification and determination of amatoxins in plasma, urine, serum, and tissue samples, with high sensitivity, specificity, and reproducibility compared to LC with UV and ECD, enzyme-linked immunoassay, and capillary electrophoresis methods.

Determination of Sesamin and Sesamolin in Sesame (Sesamum indicum L.) Seeds Using UV Spectrophotometer and HPLC

  • Kim, Kwan-Su;Lee, Jung-Ro;Lee, Joon-Seol
    • 한국작물학회지
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    • 제51권1호
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    • pp.95-100
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    • 2006
  • Sesamin and sesamolin, antioxidant lipidsoluble lignan compounds, are abundant in sesame (Sesamum indicum L.) seed oil and provide oxidative stability of oil related to sesame quality. The sesamin and sesamolin contents of 403 sesame land races of Korea were determined by HPLC analysis of methanol extract (HPLC value), and their total lignan content was compared with those by using UV-Vis spectrophotometric analysis (UV method) of methanol (UV-MeOH value) and hexane (UV-Hexane value) extracts. HPLC values of total lignan content were strongly associated with UV-Hexane (r=0.705**) and UV-MeOH (r=0.811**) values. The UV values from both the extracts were 3.8-4.7 times higher than those of HPLC values. Lignan content was overestimated by UV method because total compounds in the mixture solution were quantified by absorbing at the same ultraviolet wavelength as in HPLC method. UV method could more rapidly analyze small amount of sample with higher sensitivity of detection than HPLC method. Average contents of lignans in sesame germplasm evaluated in this study were $2.09{\pm}1.02mg/g$ of sesamin, and $1.65{\pm}0.61mg/g$ of sesamolin, respectively, showing significant variation for lignan components. The results showed that UV method for the determination of sesamin and sesamolin could be practically used as a faster and easier method than HPLC by using the regression equations developed in this study.

UV/IR 기반의 불꽃영상인식 활용연구 (A Study of UV/IR based Fire Image Detection Application)

  • 강민구;김승환;신대용;김은종;강대석
    • 한국정보통신학회:학술대회논문집
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    • 한국정보통신학회 2012년도 춘계학술대회
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    • pp.642-645
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    • 2012
  • 본 논문에서는 자외선 및 적외선 필터를 기반으로 불이 연소하면서 에너지를 빛과 열의 형태로 방출하는 산화 과정으로 여러 가지의 연소반응에 의해 표출 형태가 열, 전기, 연소가스, 복사 등으로 나타날 때, 이중 열복사로 빛이 방출되는 화염의 온도에 따라 각각 특성이 다른 파장을 활용한 적외선과 자외선(IR/UV) 필터를 활용한 불꽃영상 감지시스템 설계방안을 제안한다.

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폴리머 애자의 내전압 특성에 따른 부분방전 측정과 자외선 검출 (PD Measure and UV Detection in according to Withstand Voltage Characteristics of Polymer Insulator)

  • 송길목;김영석;김종서;정진수
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 2008년도 추계학술대회 논문집 전기설비전문위원
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    • pp.76-77
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    • 2008
  • The purposes of this paper ensure for the economical efficiency, accuracy, and good applications in domestic site set up the power installations. For the efficient conduct of these purposes, there are measured the partial discharge(PD) used by current probe and compared with detected signal of UV sensor. As the results, PD generated about 35% of breakdown voltage in polymer insulator. UV signal due to high voltage in polymer insulator is detected from about 1.7mV at 30kV to about 3mV at 70kV. Signal detected by ultra-sonic is increased at about 75% of breakdown voltage abruptly. It appears that error range is increased in boundary. UV sensor is needed must use from the practical and economical points of view.

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HPLC-UV를 이용한 중마황의 (+)-Pseudoephedrine과 (-)-Ephedrine의 동시분석법 개발 (Simultaneous Determination of (+)-Pseudoephedrine and (-)-Ephedrine in Ephedra intermedia by HPLC-UV)

  • 정비랑;윤유식;신순식;권용수;양희정
    • 생약학회지
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    • 제48권1호
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    • pp.93-96
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    • 2017
  • Ephedra alkaloids, (-)-ephedrine, (+)-pseudoephedrine, (-)-N-methylephedrine, (+)-N-methylpseudoephedrine, (-)-norephedrine and (+)-norpseudoephedrine, from ephedra herb are sympathomimetic agonists causing an increase of metabolism, blood pressure and perspiration. In this study, we developed the validation method of (+)-pseudoephedrine and (-)-ephedrine, two major ephedra alkaloids in Ephedra spp., by high-performance liquid chromatography-ultraviolet spectrometer (HPLC-UV). HPLC analysis was performed using a HECTOR-M C18 column operating at $35^{\circ}C$, and UV detection at 215nm. The mobile phase used a gradient flow with 25 mM SDS in water (A) and acetonitrile (B).

Design and construction of a new ultraviolet sensor using CsI deposition in the ionization chamber

  • Souri, R.;Negarestani, A.;Souri, S.;Farzan, M.;Mahani, M.
    • Nuclear Engineering and Technology
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    • 제50권5호
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    • pp.751-757
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    • 2018
  • In this article, a UV sensor that is an appropriate tool for fire detection has been designed and constructed. The structure of this UV sensor is an air-filled single-wire detector that is able to operate under normal air condition. A reflective CsI photocathode is installed at the end of the sensor chamber to generate photoelectrons in the ion chamber. An electric current is produced by accelerating photoelectrons to the anode in the electric field. The detector is able to measure the intensity of the incident UV rays whenever the current is sufficiently high. Therefore, the sensitivity coefficient of this sensor is found to be $7.67{\times}10^{-6}V/photons/sec$.

Composite Nanofilm of Polypyrrole and Mycosporine-like Amino Acids for UV sensor

  • Jin, Yinhua;Kulkarni, Atul;Qin, Hongyi;Kim, Dae-hwan;Yu, Yeong Wook;Lee, Joon Chul;Kim, Taesung;Moh, Sang Hun
    • 한국진공학회:학술대회논문집
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    • 한국진공학회 2016년도 제50회 동계 정기학술대회 초록집
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    • pp.354.2-354.2
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    • 2016
  • Mycosporine-like amino acids (MAAs) are small secondary metabolites produced by organisms that live in environments with high volumes of sunlight, is an important group of novel bioactive compounds having immense biotechnological poten-tials due to their UV screening properties and Polypyrrole (PPy) is a type of organic polymer formed by polymerization of pyrrole. A novel composite nanofilm (~60 nm) of mycosporine-like amino acid (MAA) and polypyrrole is synthesized by interfacial polymerization technique. This composite nanofilm is conductive and has strong photoresponse. A photoelectric UV sensor is fabricated by depositing the composite film onto a silicon chip. This UV sensor shows good sensitivity, selectivity and stability for UV detection.

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