• Journal of Microbiology and Biotechnology
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• v.29 no.11
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• pp.1749-1759
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• 2019

Aspergillus ochraceus biofilm, developed on an inert support, can produce tannase in Khanna medium containing 1.5% (w/v) tannic acid as the carbon source, at an initial pH of 5.0, for 72 h at 28℃. Addition of 0.1% (w/v) yeast extract increased enzyme production. The enzyme in the crude filtrate exhibited the highest activity at 30℃ and pH 6.0. At 50℃, the half-life (T50) was 60 min and it was 260 min at pH 6.0. In general, addition of detergents and surfactants did not affect tannase activity significantly. Tannase has potential applications in various biotechnological processes such as the production of propyl gallate and in the treatment of tannin-rich effluents. The content of tannins and total phenolic compounds in effluents from leather treatment was reduced by 56-83% and 47-64%, respectively, after 2 h of enzyme treatment. The content of tannins and total phenolic compounds in the sorghum flour treated for 120 h with tannase were reduced by 61% and 17%, respectively. Interestingly, the same A. ochraceus biofilm was able to produce tannase for three sequential fermentative process. In conclusion, fungal biofilm is an interesting alternative to produce high levels of tannase with biotechnological potential to be applied in different industrial sectors.

• BMB Reports
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• v.42 no.3
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• pp.166-171
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• 2009

Hsp70s assist in the process of protein folding through nucleotide-controlled cycles of substrate binding and release by alternating from an ATP-bound state in which the affinity for substrate is low to an ADP-bound state in which the affinity for substrate is high. It has been long recognized that the two-domain structure of Hsp70 is critical for these regulated interactions. Therefore, it is important to obtain information about conformational changes in the relative positions of Hsp70 domains caused by nucleotide binding. In this study, analytical ultracentrifugation and dynamic light scattering were used to evaluate the effect of ADP and ATP binding on the conformation of the human stress-induced Hsp70.1 protein. The results of these experiments showed that ATP had a larger effect on the conformation of Hsp70 than ADP. In agreement with previous biochemical experiments, our results suggest that conformational changes caused by nucleotide binding are a consequence of the movement in position of both nucleotide- and substrate-binding domains.

• The Transactions of the Korean Institute of Power Electronics
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• v.9 no.2
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• pp.187-193
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• 2004

For a power source of usual electronic devices such as PDA, smart phone, UPS and electric vehicle, the battery made of serially connected multiple cells is generally used. In this case, if there are some unbalanced among cell voltages, the total lifetime and the total capacity of the battery are limited to a lower value. To maintain a balanced condition in cells, an effective method of regulating the cell voltage in indispensable. In this paper, we propose the design of a balancing circuit for electronic appliances. The balancing system was controlled by a micro-controller which enables to implement the balancing action during charging period. Proposed method has been verified by the experiment using the charger and recorder. The experimental results show that the individual battery equalization can improve battery capacity and battery lifetime and performance through an extended operational time.

• Journal of Veterinary Clinics
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• v.21 no.4
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• pp.406-408
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• 2004

A nine-month-old male Jindo with non weight-bearing on the right forelimb in flexed position, pain and edema of the elbow, and resist elbow extension was brought to the Veterinary Teaching Hospital, Chungbuk National University. Elbow radiographs showed loss of humeroradial joint space and lateral displacement of the radius and ulna. Closed reduction was reported the best therapy in most cases of luxation of the elbow but conservative reduction was impossible. Open reduction of the luxated elbow was performed and ruptured collateral ligaments were identified. Displaced elbow was required bloody surgical operation and gentle reduction to restore elbow joint. Internal reduction of choice for elbow luxation with rupture of collateral ligament in the dog was a double locking-loop suture pattern. To ensure secure grasping of parallel bundles of ligament fibers to transverse bites of each suture were placed superficial to the longitudinal bites. All ligaments were repaired with 3-metric (size 2 USP) monofilament polypropylene suture. No complications have been noted during a five-month follow up.

• Journal of Pharmaceutical Investigation
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• v.30 no.3
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• pp.223-228
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• 2000

Testing the volume of injection in container is to ensure that the nominal content of a container can be administered to the patient. The method to test the volume of injection in container is described in European Pharmacopeia (EP) and United States Pharmacopeia (USP). However there is no method to test the volume of injection in Korean Pharmacopeia (KP). So we surveyed the method practiced by 53 pharmaceutical companies in Korea by a questionnaire and tested commercially available injections by discharged volume. As the result, we agreed with the International Conference on Harmonization (ICH) test method for discharged volume. It was suggested that the ICH test method for discharged volume of injection in container would be adopted.

• YAKHAK HOEJI
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• v.43 no.5
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• pp.606-613
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• 1999

To survey the possibility of replacing the pyrogen test with Limulus Amebocyte Lysate(LAL) test and to find out a standard methods suitable to our blood products made in Korea, 100 samples of 20% human serum albumin were tested by commercial LAL test kits and results of those were compared with rabbit pyrogen test. The LAL test is used both dinetic-chromogenically and kinetic-turbidimetrically. Both methods equally showed broad detection range (5.0~0.005 EU/ml), excellent sensitivity ($\geq$ 0.005 EU/ml) and predominant recovery rate within valid dilution range, but kinetic-turbidimetric method seemed to be more reproducible than kinetic-chromogenic method(kinetic-chromogenic method : S.D. = 15.88, kinetic-turbidimetric method : S.D. = 8.12). After heating the sample at 75$^{\circ}C$ for 15 min, the results showed a little elevated recovery rate with both methods. After performing the test on 100 albumin samples with both kits, the results were analysed using the USP standard (1.33 EU/ml). 7% of samples in kinetic-chromogenic methods and 1% of samples in kinetic-turbidimetric method exceeded the limit of endotoxin levels regulated for blood products in USA. Because this phenomenon was not observed in both methods at the same time and both methods have high sensitivity ($\geq$0.005 EU/ml), these results seemed to depend on nonspecific reaction. Considering its sensitivity and reproducibility, we could assure that LAL test is proper to detecting pyrogenic with good sensitivity.

• The Korean Journal of Physiology and Pharmacology
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• v.20 no.5
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• pp.487-498
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• 2016

Leptin, an adipokine predominantly produced from adipose tissue, is well known to induce tumor growth. However, underlying molecular mechanisms are not established yet. While p53 has long been well recognized as a potent tumor suppressor gene, accumulating evidence has also indicated its potential role in growth and survival of cancer cells depending on experimental environments. In the present study, we examined if p53 signaling is implicated in leptin-induced growth of cancer cells. Herein, we demonstrated that leptin treatment significantly increased p53 protein expression in both hepatic (HepG2) and breast (MCF-7) cancer cells without significant effect on mRNA expression. Enhanced p53 expression by leptin was mediated via modulation of ubiquitination, in particular ubiquitin specific protease 2 (USP2)-dependent manner. Furthermore, gene silencing of p53 by small interfering RNA (siRNA) suppressed leptin-induced growth of hepatic and breast cancer cells, indicating the role of p53 signaling in tumor growth by leptin. In addition, we also showed that knockdown of p53 restored suppression of caspase-3 activity by leptin through modulating Bax expression and prevented leptin-induced cell cycle progression, implying the involvement of p53 signaling in the regulation of both apoptosis and cell cycle progression in cancer cells treated with leptin. Taken together, the results in the present study demonstrated the potential role of p53 signaling in leptin-induced tumor growth.

• Journal of Radiopharmaceuticals and Molecular Probes
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• v.5 no.1
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• pp.48-53
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• 2019

A bone metastasis is an important factor for prognosis and treatment of breast or prostate cancer patients. [$^{18}F$]Sodium fluoride ([$^{18}F$]NaF) is a PET radiopharmaceutical that can detect bone metastasis. Conventional [$^{18}F$]NaF production process included radioactive metal impurities because the product was prepared by adding saline after beam irradiation to $[^{18}O]H_2O$. In this study, we apply the method of removing radionuclidic impurities. To meet the criteria prescribed by GMP in quality control, we designed the custom-made [$^{18}F$]NaF automatic module. The mean radiochemical yield was $82.1{\pm}4.4%$ (n = 32) productions for 3 years) and the total preparation time was 4 min. The final produced [$^{18}F$]NaF solution meets the USP criteria for quality control. Thus, this fully automated system is validated for clinical use.

• Journal of Smart Tourism
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• v.2 no.2
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• pp.21-32
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• 2022

This paper shows light on the concept of smart tourism destination as a future of tourism development, especially in the context of problems associated with developing countries. This study substantiates the case of smart tourism development in mountain destinations as an affordable and needed future of the contemporary era. This conceptual study is based on secondary literature on Smart Cities, Smart Tourism Destinations, and Tourism in Himachal Pradesh. The Indian state of Himachal Pradesh has been considered as a study area to acknowledge major tourism-related challenges, especially in mountain locations. Consequently, the dots are connected between existing challenges and solutions that smart tourism holds. It turns out that for the development of mountain tourism destinations such as Himachal Pradesh in the Indian Himalayas, investments in smart infrastructure are required. By developing smart infrastructure, a new USP can be made, a supportive environment for new local businesses, new employment opportunities, enhanced tourist experience and an overall raised standard of living for locals. Considering all factors, it leads to a highly competitive tourism destination. All tourism destinations located in the Himalayan mountains show somewhat the same tourism challenges as Himachal Pradesh, India. Therefore, this paper brightens the path of destination planners towards the development agenda of smart tourism destinations and shows how smart tourism infrastructure can be deployed for better management of tourism destinations.

• Steel and Composite Structures
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• v.43 no.2
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• pp.241-256
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• 2022

In this article, an analytical procedure is presented for static analysis of composite cylinders with the geometrically nonlinear behavior, and non-uniform thickness profiles under different loading conditions by considering moderately large deformation. The composite cylinder includes two inner and outer isotropic layers and one honeycomb core layer with adjustable Poisson's ratio. The Mirsky-Herman theory in conjunction with the von-Karman nonlinear theory is employed to extract the governing equations which are a system of nonlinear differential equations with variable coefficients. The governing equations are solved analytically using the matched asymptotic expansion (MAE) method of the perturbation technique and the effects of moderately large deformations are studied. The presented method obtains the results with fast convergence and high accuracy even in the regions near the boundaries. Highlights: • An analytical procedure based on the matched asymptotic expansion method is proposed for the static nonlinear analysis of composite cylindrical shells with a honeycomb core layer and non-uniform thickness. • The effect of moderately large deformation has been considered in the kinematic relations by assuming the nonlinear von Karman theory. • By conducting a parametric study, the effect of the honeycomb structure on the results is studied. • By adjusting the Poisson ratio, the effect of auxetic behavior on the nonlinear results is investigated.