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Applications of Enzyme Immobilized Membranes: A Review (효소 고정화막의 응용에 대한 총설)

  • Ryu, Junghyun;Patel, Rajkumar;Kim, Jong Hak
    • Membrane Journal
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    • v.31 no.6
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    • pp.393-403
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    • 2021
  • Enzymes are important class of catalyst for biotransformation. Stability and reusability of enzymes during the catalysis process is a key issue. Activity of enzyme can be enhanced by its immobilization on a suitable substrate by creation of specific microenvironment. A variety of membranes has been used as substrate due to the biocompatibility and simpler method to tune hydrophilicity/hydrophobicity property of the membrane surface. In this review, polymer membranes including cellulose, polyacrylonitrile (PAN), polydimethylsiloxane (PDMS), polyvinylidene fluoride (PVDF), polyethersulfone (PES) are introduced and discussed in detail. Biodegradation of organic contaminants by immobilized enzyme is an environmental friendly process to reduce the contamination of environment in pharmaceutical company and textile industries. The controlled hydrolysis of oil can be performed in enzyme immobilized membrane bioreactor (EMBR), resulting in reducing carbon emission and reduced environmental pollution. Bioethanol and biodiesel are considered alternative fossil fuels that can be prepared in EMBR.

Transarterial Embolization of Intracranial Arteriovenous Fistulas with Large Venous Pouches in the Form of Venous Outlet Ectasia and Large Venous Varix or Aneurysm : Two Centers Experience

  • Deniwar, Mohamed Adel;Ahmad, Saima;Eldin, Ashraf Ezz
    • Journal of Korean Neurosurgical Society
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    • v.65 no.1
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    • pp.30-39
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    • 2022
  • Objective : There are different types of cerebral vascular malformations. Pial arteriovenous fistulas (PAVFs) and dural arteriovenous fistulas (DAVFs) are two entities; they consist of one or more arterial connections to a single venous outlet without a true intervening nidus. The high turbulent flow of PAVFs and aggressive DAVFs with cortical venous reflux can result in venous outflow varix and aneurysmal dilatation. They pose a significant challenge to transvenous embolization (TVE), stereotactic radiosurgery, and surgical treatment. We aim to share our centers' experience with the transarterial embolization (TAE) for arteriovenous fistulas (AVFs) with large venous pouches and to report the outcome. Methods : The authors' two institutions' databases were retrospectively reviewed from February 2017 to February 2021. All patients with intracranial high flow PAVFs and aggressive DAVFs with venous outlet ectasia and large venous varix and were treated by TAE were included. Results : Fifteen patients harboring 11 DAVFs and four PAVFs met our inclusion criteria. All patients underwent TAE in 17 sessions. Complete angiographic obliteration was achieved after 14 sessions in 12 patients (80%). Four patients (25%) had residual after one TAE session. Technical failure was documented in one patient (6.7%). Fourteen patients (93.3%) had favorable functional outcome (modified Rankin score 0-2). Conclusions : TAE for high flow or aggressive intracranial AVFs is a safe and considerable treatment option, especially for those associated with large venous pouches that are challenging and relatively high-risk for TVE.

The development of EASI-based multi-path analysis code for nuclear security system with variability extension

  • Andiwijayakusuma, Dinan;Setiadipura, Topan;Purqon, Acep;Su'ud, Zaki
    • Nuclear Engineering and Technology
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    • v.54 no.10
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    • pp.3604-3613
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    • 2022
  • The Physical Protection System (PPS) plays an important role and must effectively deal with various adversary attacks in nuclear security. In specific single adversary path scenarios, we can calculate the PPS effectiveness by EASI (Estimated Adversary Sequence Interruption) through Probability of Interruption (PI) calculation. EASI uses a single value of the probability of detection (PD) and the probability of alarm communications (PC) in the PPS. In this study, we develop a multi-path analysis code based on EASI to evaluate the effectiveness of PPS. Our quantification method for PI considers the variability and uncertainty of PD and PC value by Monte Carlo simulation. We converted the 2-D scheme of the nuclear facility into an Adversary Sequence Diagram (ASD). We used ASD to find the adversary path with the lowest probability of interruption as the most vulnerable paths (MVP). We examined a hypothetical facility (Hypothetical National Nuclear Research Facility - HNNRF) to confirm our code compared with EASI. The results show that implementing the variability extension can estimate the PI value and its associated uncertainty. The multi-path analysis code allows the analyst to make it easier to assess PPS with more extensive facilities with more complex adversary paths. However, the variability of the PD value in each protection element allows a significant decrease in the PI value. The possibility of this decrease needs to be an important concern for PPS designers to determine the PD value correctly or set a higher standard for PPS performance that remains reliable.

Membrane Containing Biocidal Material for Reduced Biofilm Formation: A Review (미생물막 형성을 막기 위한 살균 물질 함유 막: 총설)

  • Son, Soohyun;Patel, Rajkumar
    • Membrane Journal
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    • v.32 no.1
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    • pp.23-32
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    • 2022
  • Bacteria grow biofilm on various surface such as separation membrane, food packaging film and biomedical device. Growth of biofilm is associated with the formation of a complex structure of exopolysaccharides. Effect of antibacterial effect reduce drastically once the biofilm developed due to the difficulties in mass transport of antimicrobial agent. In order to enhance the antibacterial activity, surface of the membrane is modified, coated or immobilized with functional materials with biocidal properties. One of the idea is to introduce positive charge on the membrane surface by the presence of quaternary ammonium group which might displace divalent metal ion such as magnesium or calcium present in the bacteria cell wall. Efficacy of cell membrane disruption depends on the mobility of the agents available directly on the surface environment. In this review, various biocidal agents like quaternary ammonium group, helamine or zwitter ion containing membrane are discussed.

On vibration and flutter of shear and normal deformable functionally graded reinforced composite plates

  • Abdollahi, Mahdieh;Saidi, Ali Reza;Bahaadini, Reza
    • Structural Engineering and Mechanics
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    • v.84 no.4
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    • pp.437-452
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    • 2022
  • For the first time, the higher-order shear and normal deformable plate theory (HOSNDPT) is used for the vibration and flutter analyses of the multilayer functionally graded graphene platelets reinforced composite (FG-GPLRC) plates under supersonic airflow. For modeling the supersonic airflow, the linear piston theory is adopted. In HOSNDPT, Legendre polynomials are used to approximate the components of the displacement field in the thickness direction. So, all stress and strain components are encountered. Either uniform or three kinds of non-uniform distribution of graphene platelets (GPLs) into polymer matrix are considered. The Young modulus of the FG-GPLRC plate is estimated by the modified Halpin-Tsai model, while the Poisson ratio and mass density are determined by the rule of mixtures. The Hamilton's principle is used to obtain the governing equations of motion and the associated boundary conditions of the plate. For solving the plate's equations of motion, the Galerkin approach is applied. A comparison for the natural frequencies obtained based on the present investigation and those of three-dimensional elasticity theory shows a very good agreement. The flutter boundaries for FG-GPLRC plates based on HOSNDPT are described and the effects of GPL distribution patterns, the geometrical parameters and the weight fraction of GPLs on the flutter frequencies and flutter aerodynamic pressure of the plate are studied in detail. The obtained results show that by increasing 0.5% of GPLs into polymer matrix, the flutter aerodynamic pressure increases approximately 117%, 145%, 166% and 196% for FG-O, FG-A, UD and FG-X distribution patterns, respectively.

Evaluation of Edge-Based Data Collection System for Key-Value Store Utilizing Time-Series Data Optimization Techniques (시계열 데이터 최적화 기법을 활용한 Key-value store의 엣지 기반 데이터 수집 시스템 평가)

  • Woojin Cho;Hyung-ah Lee;Jae-hoi Gu
    • The Journal of the Convergence on Culture Technology
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    • v.9 no.6
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    • pp.911-917
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    • 2023
  • In today's world, we find ourselves facing energy crises due to factors such as war and climate crises. To prepare for these energy crises, many researchers continue to study systems related to energy monitoring and conservation, such as energy management systems, energy monitoring, and energy conservation. In line with these efforts, nations are making it mandatory for energy-consuming facilities to implement these systems. However, these facilities, limited by space and energy constraints, are exploring ways to improve. This research explores the operation of a data collection system using low-performance embedded devices. In this context, it proves that an optimized version of RocksDB, a Key-Value store, outperforms traditional databases when it comes to time-series data. Furthermore, a comprehensive database evaluation tool was employed to assess various databases, including optimized RocksDB and regular RocksDB. In addition, heterogeneous databases and evaluations are conducted using a UD Benchmark tool to evaluate them. As a result, we were able to see that on devices with low performance, the time required was up to 11 times shorter than that of other databases.

The Study of Diagnostic Results Discordance Analysis on BMD Using DEXA (이중에너지 X선 흡수 계측법을 이용한 골밀도 검사 시 진단불일치에 대한 분석)

  • Park, Won-Kyu;Kang, Yeong-Han;Jo, Gwang-Ho
    • Journal of radiological science and technology
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    • v.31 no.1
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    • pp.25-31
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    • 2008
  • Purpose : This study was conducted to understand for the diagnostic discordance of bone mineral density(BMD) in DEXA(Dual Energy X-ray Absorptiometry). And the diagnostic difference between lumbar spine and femur neck, forearm was evaluated using T-score. Materials and Methods : We studied 220 females measured BMD on lumbar spine, and femur neck, forearm including ward's triangle and ultra digital(UD). We were distinguished T-score into normal, osteopenia, osteoporosis(WHO classification) and evaluated discordance rate according to age and degree of bone loss. Correlation analysis and chi-square test between L-spine, L-4, femur neck, Ward, Forearm, UD were carried out. Results : In the lumbar spine, the number of normal were in 57(25.9%), osteopenia in 86(39.1%), osteoporosis in 77(35.0%). In the L-4 and ward's triangle, the number of osteoporosis were in 78(35.5%), in 126(57.3%). There was significant correlation between lumbar, femur neck and forearm BMD in all cases. The discordance of BMD between lumbar and femur were 57%, lumbar and forearm 43%, forearm and femur 51%. The discordance rates of normal, osteopenic, osteoporotic groups were 39%, 64%, 43%, respectively, showing the highest discordance rate in osteopenia patients. In normal group of lumbar spine, the discordance rate was 25%, 23%, 11%, 65%, 86% in 30', 40', 50', 60', 70', respectively. In osteopenia, osteoporosis group of lumbar spine, the discordance rate was 62%, 55%, 36%, 20%, 9% in 30', 40', 50', 60', 70', respectively. Conclusion : It was different of the results of BMD with lumbar, femur and forearm site. The discordance rate was decreased with age in osteopenia, osteoporosis lumbar spine. In osteopenia group, the discordance rate was the highest. So, it is necessary that the BMD of lumbar, femur neck and forearm should be checked.

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Estimation of Genetic Parameters for Economic Traits and Profit by Milk Production of Holstein Dairy Cattle in Korea (국내 Holstein종 젖소의 경제형질과 착유량에 따른 소득의 유전모수 추정)

  • Noh, Jae-Kwang;Choi, Yun-Ho;Cho, Kwang-Hyun;Choi, Tae-Jeong;Na, Seung-Hwan;Cho, Ju-Hyun;Kim, Jin-Hyung;Shin, Ji-Sub;Do, Chang-Hee
    • Journal of Animal Science and Technology
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    • v.54 no.4
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    • pp.275-282
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    • 2012
  • The data including milk yields, fat and protein percent for 628,395 heads collected by National Agricultural Cooperative Federation, 15 type traits and final score for 62,262 heads collected by Korea Animal Improvement Association, which were born in 1998 to 2004, and net profits calculated from milk price and raising expenses of individuals were used to estimate genetic parameters. The highest positive genetic correlation, 0.81, was shown between body depth (BD) and loin strength (SR). Genetic correlations between body depth (BD) and udder depth (UD), front teat placement (TP) and front teat length (TL) were -0.23, which were lowest among the linear type traits. Furthermore, medium level of negative genetic correlations were shown the milk yield with milk contents rate traits. Mostly low level of positive genetic correlations were shown between the milk traits and linear score traits except milk yield and stature. Most of the genetic correlations of between the linear score traits and net profit were low level of positive or negative genetic correlations. Among the genetic correlations, body depth (BD), angularity (DF) and rear attachment width (UW), and final score (FS) with net profit were high as 0.17, 0.17, 0.18 and 0.18, respectively. Finally all of the genetic correlations between net profit and milk traits were positive and higher than the linear traits with positive genetic correlations. The results of this study suggest that net profit has been related with the linear traits, such as body depth (BD), angularity (DF) and rear attachment width (UW) traits, and furthermore, milk traits including yield and contents rates influence positively and greatly on net profit.

How Our Practice of Histopathology, Especially Tumour Pathology has Changed in the Last Two Decades: Reflections from a Major Referral Center in Pakistan

  • Ahmad, Zubair;Idrees, Romana;Fatima, Saira;Arshad, Huma;Din, Nasir-Ud;Memon, Aisha;Minhas, Khurram;Ahmed, Arsalan;Fatima, Syeda Samia;Arif, Muhammad;Ahmed, Rashida;Haroon, Saroona;Pervez, Shahid;Hassan, Sheema;Kayani, Naila
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.9
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    • pp.3829-3849
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    • 2014
  • Continued advances in the field of histo pathology (and cyto pathology) over the past two decades have resulted in dramatic changes in the manner in which these disciplines are now practiced. This is especially true in the setting of a large university hospital where the role of pathologists as clinicians (diagnosticians), undergraduate and postgraduate educators, and researchers has evolved considerably. The world around us has changed significantly during this period bringing about a considerable change in our lifestyles and the way we live. This is the world of the internet and the world-wide web, the world of Google and Wikipedia, of Youtube and Facebook where anyone can obtain any information one desires at the push of a button. The practice of histo (and cyto) pathology has also evolved in line with these changes. For those practicing this discipline in a poor, developing country these changes have been breathtaking. This is an attempt to document these changes as experienced by histo (and cyto) pathologists practicing in the biggest center for Histopathology in Pakistan, a developing country in South Asia with a large (180 million) and ever growing population. The Section of Histopathology, Department of Pathology and Microbiology at the Aga Khan University Hospital (AKUH) in Karachi, Pakistan's largest city has since its inception in the mid-1980s transformed the way histopathology is practiced in Pakistan by incorporating modern methods and rescuing histopathology in Pakistan from the primitive and outdated groove in which it was stuck for decades. It set histopathology in Pakistan firmly on the path of modernity and change which are essential for better patient management and care through accurate and complete diagnosis and more recently prognostic and predictive information as well.

Irradiation enduced In-plane magnetization in Fe/MgO/Fe/Co multilayers

  • Singh, Jitendra Pal;Lim, Weon Cheol;Song, Jonghan;Kim, Jaeyeoul;Asokan, K.;Chae, Keun Hwa
    • Proceedings of the Korean Vacuum Society Conference
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    • 2015.08a
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    • pp.188.1-188.1
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    • 2015
  • For present investigation Fe/MgO/Fe/Co multilayer stack is grown on Si substrate using e-beam evaporation in ultrahigh vacuum. This stack is irradiated perpendicularly by 120 MeV $Ag^{8+}$ at different fluences ranging from $1{\times}10^{11}$ to $1{\times}10^{13}ions/cm^2$ in high vacuum using 15UD Pelletron Accelerator at Inter University Accelerator Centre, New Delhi. Magnetic measurements carried out on pre and post irradiated stacks show significant changes in the shape of perpendicular hysteresis which is relevant with previous observation of re-orientation of magnetic moment along the direction of ion trajectory. However increase in plane squareness may be due to the modification of interface structure of stacks. X-ray reflectivity measurements show onset of interface roughness and interface mixing. X-ray diffraction measurements carried out using synchrotron radiation shows amorphous nature of MgO and Co layer in the stack. Peak corresponding body centered Fe [JCPDS-06-0696] is observed in X-ray diffraction pattern of pre and post irradiated stacks. Peak broadening shows granular nature of Fe layer. Estimated crystallite size is $22{\pm}1nm$ for pre-irradiated stack. Crystallite size first increases with irradiation then decreases. Structural quality of these stacks was further studied using transmission electron microscopic measurements. Thickness from these measurements are 54, 36, 23, 58 and 3 nm respectively for MgO, Fe, MgO, Fe+Co and Au layers in the stack. These measurements envisage poor crystallinity of different layers. Interfaces are not clear which indicate mixing at interface. With increase fluence mixing and diffusion was increased in the stack. X-ray absorption spectroscopic measurements carried out on these stacks show changes of Fe valence state after irradiation along with change of O(2p)-metal (3d) hybridized state. Valence state change predicts oxide formation at interface which causes enhanced in-plane magnetization.

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