• Molecules and Cells
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• 제39권9호
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• pp.680-686
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• 2016

Uncoupling proteins (UCPs) are mitochondrial inner membrane proteins that function to dissipate proton motive force and mitochondrial membrane potential. One UCP has been identified in Caenorhabditis elegans (C. elegans), namely UCP-4. In this study, we examined its expression and localization using a GFP marker in C. elegans. ucp-4 was expressed throughout the body from early embryo to aged adult and UCP-4 was localized in the mitochondria. It is known that increased mitochondrial membrane protential leads to a reactive oxygen species (ROS) increase, which is associated with age-related diseases, including neurodegenerative diseases in humans. A ucp-4 mutant showed increased mitochondrial membrane protential in association with increased neuronal defects during aging, and the neurons of ucp-4 overexpressing animals showed decreased neuronal defects during aging. These results suggest that UCP-4 may be involved in neuroprotection during aging via relieving mitochondrial membrane protential. We also investigated the relationship between UCP-4 and innate immunity because increased ROS can affect innate immunity. ucp-4 mutant displayed increased resistance to the pathogen Staphylococcus aureus compared to wild type. The enhanced immunity in the ucp-4 mutant could be related to increased mitochondrial membrane protential, presumably followed by increased ROS. In summary, UCP-4 might have an important role in neuronal aging and innate immune responses through mediating mitochondrial membrane protential.

• 통상정보연구
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• 제4권1호
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• pp.39-57
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• 2002

전자신용장통일규칙에 대한 그 동안의 논의는 주로 UCP500과는 별도로 새로운 통일규칙을 제정하여야 한다는 논의와 기존의 UCP500내에서 전자서류에 대한 내용을 새로이 추가하여야 한다는 논의였다. 그러나 궁극적으로 UCP500의 경우 종이서류를 근거로 하여 내용을 규정하고 있기 때문에 전자신용장거래에서의 전자제시에 대하여 적용할 수 없었다. 이러한 점에서 UCP500에서는 전자무역거래에서 사용되고 있는 기본 개념조차도 수용되어 있지 않고 있는데 예를 들면, 전자서명, 전자서류 또는 전자기록에 대한 내용이다. UCP가 안고 있는 이러한 문제의 소지를 사전에 해결하기 위하여 ICC에서 eUCP를 제정하였다는 점에서 큰 의의가 있다고 할 수 있다. 이와 같이 본 연구는 eUCP의 도입배경을 선화증권의 위기문제와 연관시켜 조명하고 eUCP의 주요내용을 살펴보고 이를 UCP500의 규정과 비교하여 봄으로써 UCP500과 유기적으로 사용될 eUCP의 이해를 제고하여 향후 발생될 당사자의 분쟁을 사전에 방지하고자 하는 것이 그 목적이다.

• 통상정보연구
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• 제4권2호
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• pp.13-26
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• 2002

본 논문에서는 eUCP에서 전자자료 제시의 기준과 심사기준을 중심으로 연구하였다. 전자자료 제시의 장소, 제시의 방법에 대한 기준과 제시시기 및 전자제시에 대한 인증에서 발생하는 문제점을 제기하고 심사기간의 기준과 하이퍼링크방식의 전자자료에 대한 심사기준, 훼손된 전자기록에 대한 심사기준을 분석하였으며, 전자가록의 거절의 방법과 그에 대한 응답에 관련된 내용을 분석하였다. 전자제시의 문제점으로서 표준양식의 문제, 전자제시자의 면책의 문제, 환어음 유통성의 문제, 서류 심사기간, 인증기관과 방법에 관한 문제에 대하여 분석하여 해결방안을 제시하였다.

• 무역상무연구
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• 제12권
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• pp.405-427
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• 1999

Although Uniform Customs and practice for Documentary(UCP) is not a law, it applies to most documentary credits and is binding on all parties unless otherwise expressly stipulated. Besides, Uniform Commercial Code(UCC) Article 5 was codified by the United States and was adopted by every state. Moreover, the New York version of the UCC Article 5-102(4) specifically providing that the UCC does not apply to letters of credit where the parties agree to be governed by the UCP. Identical nonuniform Articles were latter added in Alabama, Arizona, and Missouri. The fact that courts in forty-six of the fifty states are bound by Article 5. Until now, Article 5 of the UCC has probably had an impact on the decisions in New York and the New York common law. Therefore, I examined a few issues on application between Article 5 of the UCC and the UCP. First, although the UCP attempt to introduce a new for examination of document by incorporating "standard practice of financial institutions" and "international banking practice", the standards for documentary compliance are not clear. The UCC attempt to rely on the matter of interpretation for the court, but the UCP would probably be interested in examining in about bank's internal practices as reflected in UCP Articles. Second, the rule for nondocumentary conditions is a useful for stand-by credit transactions under the UCC, but these conditions would probably put the bank in an even worse position in case of documentary credit transactions under the UCP. Third, the UCP does not contain any provision governing the fraud exception, but the UCC codified the fraud and forgery rules developed through American case law. Fourth, the UCP treats the issue of transfer in much more detail than the UCC does. In contract, the UCP's treatment of assignment of proceeds is brief. Finally, I suggest that the fraud exception rules should be prescribed in the UCP in order to protect the issuing bank and the applicant when an unscrupulous party attempts to defraud.

• BMB Reports
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• 제39권4호
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• pp.346-354
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• 2006

The full-length cDNA of grass carp (Ctenopharyngodon idellus) and silver carp (Hypophthalmichthys molitrix) uncoupling protein 2 (UCP2) was obtained from liver. The grass carp UCP2 cDNA was determined to be 1152 bp in length with an open reading frame that encodes 310 amino acids. Five introns (Intron 3, 4, 5, 6 and 7) in the translated region, and partial sequence of Intron 2 in the untranslated region of grass carp UCP2 gene were also obtained. Gene structure comparison between grass carp and mammalian (human and mouse) UCP2 gene shows that, the UCP2 gene structure of grass carp is much similar to that of human and mouse. Partial UCP2 cDNA sequences of bighead carp (Aristichthys nobilis) and mud carp (Cirrhinus molitorella), were further determined. Together with the common carp (Cyprinus carpio) UCP2 sequence from GenBank (AJ243486), multiple alignment result shows that the nucleotide and amino acid sequences of the UCP2 gene, were highly conserved among the five major Chinese carps that belong to four subfamilies. Using beta-actin as control, the ratio UCP2/beta-actin mRNA (%) was determined to be $149.4{\pm}15.6$ (common carp), $127.4{\pm}22.1$ (mud carp), $96.7{\pm}12.7$ (silver carp), $94.1{\pm}26.8$ (bighead carp) and $63.7{\pm}16.2$ (grass carp). The relative liver UCP2 expression of the five major Chinese carps, shows a close relationship with their food habit: benthos and detrituseating fish (common carp and mud carp) > planktivorious fish (silver carp and bighead carp) > herbivorious fish (grass carp). We suggest that liver UCP2 might be important for Chinese carps to detoxify cyanotoxins and bacteria in debris and plankton food.

• Journal of Nutrition and Health
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• 제41권8호
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• pp.767-775
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• 2008

1) 연구대상자 중 비만아동 빈도수 분석 결과, BIA법에서는 70.0%, BMI에 의한 비만도에서는 32.7%, 신장별 체중에 의한 비만도에서는 23.6%로 BAI법에 의해 비만아동의 빈도수가 가장 많았다. 혈청 생화화적 분석 결과는 NCEP의 이상지혈증 기준 이하로 나타났다. 2) UCP-1 유전자 다형성에 의한 신체계측의 결과의 분포는 정상형과 변이형에서 차이를 나타내지 않았고 혈중 생 화학 결과에서는 LDL (p = 0.039)과 TC (p = 0.063)이 정상형에 비해 변이형에서 유의적으로 증가하였다. 3) LDL을 130 mg/dL 기준으로 고LDL 콜레스테롤혈증과 정상으로 나누었을 때 각각에서 UCP-1 유전자 다형성의 분포는 고LDL 콜레스테롤혈증에서 A allele는 5.4%, G allele는 13.0%로 G allele의 빈도가 높게 분포하였고, 정상에서는 각각 94.6%, 87%의 분포를 나타내었다 (p = 0.062, ORs 2.640). LDL의 농도를 백분위수에 따라 4집단으로 나누고 UCP-1 유전자의 A allele와 G allele에서 LDL 농도의 빈도수를 나타내었을 때 G allele에서는 빈도수가 25th, 50th, 75th, 100th에 따라 유의적으로 증가하였다 ($r^2$ = 0.7995, p-trend = 0.032). 따라서 UCP-1 유전자 변이형은 정상형에 비해 고LDL-콜레스테롤혈증의 발병을 증가시키는 것으로 나타났다. UCP-1 유전자의 다형성은 이상지혈증의 위험인자인 LDL 농도를 증가시키는 위험요인으로써 나타났고, 체중을 감안할 때는 이상지혈증의 위험도가 더욱 증가할 것으로 생각되어진다. 따라서 UCP-1 유전자 변이형을 가지는 비만아동은 고LDL-콜레스테롤혈증의 발병과 관련된 식이 및 환경적 인자를 적절히 통제하는 예방 대책을 마련하여야겠다.

• 대한의생명과학회지
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• 제14권4호
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• pp.239-242
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• 2008

Obesity results from a combination of genetic, environmental, and behavioral factors. Uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCP separates oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCP facilitates the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. Therefore, we investigated the genotype for the G>A polymorphism at the position -866 of UCP2 gene in Koreans and compared genotype of patients with control group. 50 patients (Male 22, Female 28), who previously underwent type 2 diabetcs (T2DM) and 30 controls (Male 14, Female 16) participated in this study. There was a weak significant association between -866 G>A polymorphism in UCP2 gene and T2DM. The present study shows that UCP2 -866 G>A polymorphism may not be associated with the pathogenesis of T2DM as opposed to the previous reports in other countries. Further studies with larger population may be needed for the development of diagnostic methods at genetic level such as DNA chip.

• Molecules and Cells
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• 제40권11호
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• pp.864-870
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• 2017

The uncoupling protein 4 (ucp-4) gene is involved in age-dependent neurodegeneration in C. elegans. Therefore, we aimed to investigate the mechanism underlying the association between mitochondrial uncoupling and neurodegeneration by examining the effects of uncoupling agents and ucp-4 overexpression in C. elegans. Treatment with either DNP or CCCP improved neuronal defects in wild type during aging. Uncoupling agents also restored neuronal phenotypes of ucp-4 mutants to those exhibited by wild type, while ucp-4 overexpression attenuated the severity of age-dependent neurodegeneration. Neuronal improvements were further associated with reductions in mitochondrial membrane potentials. However, these age-dependent neuroprotective effects were limited in mitophagy-deficient mutant, pink-1, background. These results suggest that membrane uncoupling can attenuate age-dependent neurodegeneration by stimulating mitophagy.

• Asian-Australasian Journal of Animal Sciences
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• 제18권11호
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• pp.1552-1556
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• 2005

The UCPs are members of the mitochondrial inner membrane transporter family, present in the mitochondrial inner membrane. Their main function is increasing the energy expenditure via diminishing the resulting production of ATP from mitochondrial oxidative phosphorylation instead of yielding dissipative heat. They are associated with the metabolism of fat and regulation of energy expenditure. The UCP gene can be viewed as the candidate gene for chicken fatness. In the present study, RT-PCR and Northern Blot methods were developed to investigate the expression of the UCP gene in ten tissues including heart, liver, spleen, lung, kidney, gizzard, intestine, brain, breast muscle and abdominal fat of chicken. The results of both RT-PCR and Northern Blot methods showed that the UCP gene expressed specific in breast muscle. The expression levels of UCP gene in breast muscles from egg-type and meat-type chickens of hatching, 2, 4, 6 and 8 wk of age were detected by RT-PCR assay and results showed that the expression levels of UCP gene were related to breeds. Expression level of UCP gene in layers was higher than that in broilers at various weeks of age except at 6 wk. The UCP gene's expression was higher at 6 wk and had no significant difference among other weeks of age in broilers; in layers the expression level of UCP gene had no significant difference among weeks of age. The experiment results also showed that insulin could increase the expression level of UCP gene by 40% compared with control group.

• 대한한의학방제학회지
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• 제31권4호
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• pp.265-281
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• 2023

Objectives : Ukgan-san plus Citri Pericarpium and Pinelliae Rhizoma (UCP) is used as a traditional herbal formula in Korea and Japan for treatment of fever, fever-induced convulsions, and liver dysfunction and so on. In this study, we investigated the cytoprotective effect and underlying mechanism of UCP against oxidative stress induced by cotreatment of arachidonic acid (AA) and iron. Methods : To evaluate the hepatoprotective effects of UCP against AA + iron-induced oxidative stress in HepG2 cell, cell viability and changes on apoptosis-related proteins were assessed by MTT and immunoblot analyses. The changes in intracellular reactive oxygen species (ROS), glutathione (GSH), and mitochondrial membrane permeability (MMP) were investigated against to the oxidative stress. Furthermore, to verify underlying molecular mechanism, NF-E2-related factor 2 (Nrf2) and its downstream target genes were examined by immunoblot analysis. Results : Treatment of UCP increased the cell viability and altered the expression levels of apoptosis-related proteins such as PARP, caspase-9, caspase-3, Bcl-2. UCP also inhibited the GSH depletion, excessive ROS production and mitochondrial dysfunction induced by AA + iron. In addition, the Nrf2 and the Nrf2 target genes activation were increased by UCP. Conclusions : These results indicated that UCP has the ability to protect against oxidative stress-induced hepatocyte damage, which may be mediated with Nrf2 pathway.