• 생명과학회지
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• 제19권2호
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• pp.213-218
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• 2009

본 연구에서는 F344계 흰쥐를 대상으로 8주간 저강도 운동군과 고강도 운동군으로 나누어 수영운동을 실시하여, 갈색지방조직 내 UCP-1과 UCP-3mRNA 발현을 관찰하고 혈당 및 인슐린 수준이 어떠한 변화를 나타내는지 알아보았다. 그 결과 저강도 수영을 실시한 그룹이 대조군과 고강도 운동그룹보다 갈색지방조직 내 UCP-1과 UCP-3 mRNA 발현이 증가되는 것을 관찰하였으며, 고강도 수영군에서 대조군 보다 인슐린 수준이 낮게 나타났으나 혈당에서는 유의한차가 나타나지 않았다. 하지만 저강도 수영군에서 대조군보다 혈당 및 인슐린 수준이 유의하게 감소하는 것을 관찰하였다. 이러한 결과는 저강도 수영운동이 갈색지방조직 내 UCP-1과 UCP-3mRNA 발현을 증가시키고, 당대사를 활성화하여 인슐린민감도를 개선시킬 수 있음을 보여주는 결과이다.

• 운동영양학회지
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• 제13권2호
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• pp.155-160
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• 2009

PURPOSE. The purpose of this study was to investigate the effects of fasting and high-fat diet feeding on uncoupling protein 3 (UCP3) mRNA levels, uncoupling the respiratory chain and producing heat, of skeletal muscle in rat. METHODS. Fasting experiment: Forty Male Sprague-Dawley rats (5 wk) were divided into non-fasting groups (CON) and fasting groups (FG) for 0 day, 0.5 day (12 hr), 1 day, 2 day and 3 day. The rats of CON were sacrificed at 0 and 3 day. High-fat diet experiment: Forty Male Sprague-Dawley rats (5 wk) were divided into low-fat diet groups (LF) and high-fat diet group feeding for 0 day, 0.5 day (12 hr), 1 day, 2 day and 3 day. The rats of LF were sacrificed at 0 and 3 day. Analysis: Analysis of UCP3 mRNA expression was used by Real-time PCR. RESULTS. UCP3 mRNA levels of FG group were increased according to time course for 2 days- fasting but decreased at 3 day-fasting. UCP3 mRNA of HF were increased during HF diet feeding for 2 day, and peaked at 1 day-HF feeding, but decreased 2 day and 3 day-HF feeding CONCLUSION. Therefore, it may be rational that UCP3 is up-regulation when a large amount of fatty acids influx occurs in skeletal muscles as well as might have a role for fine adjustments of energy expenditure.

• Nutritional Sciences
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• 제7권2호
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• pp.70-75
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• 2004

The objective of this study was to examine diet-induced changes in the expression of UCP2 mRNA in the liver and UCP3 mRNA in the skeletal muscle of mice fed a high-sucrose or high-fat diet. Male ICR mice, aged 4 weeks, were divided into three dietary groups and fed control (N) or modified AIN-76 high-sucrose (US) or high-fat (HF) diets for 12 weeks. The serum total cholesterol (TC) and LDL-cholesterol concentrations of the HF group were significantly higher than those of the N and HS groups. The hepatic TC and triglyceride contents of the HS and HF groups were also significantly higher than those of the N group. The HS diet group had higher serum leptin and insulin levels compared to those of the HF group. Hepatic UCP2 mRNA expression was significantly higher in the HS group than in the N group, but the level in the HF group did not differ from that of the N group. Muscular UCP3 mRNA level was significantly higher in the HF group and especially in the HS group than in N the group. We observed that two gene (UCP2, 3) levels exhibited a similar tendency. These results suggest that UCPs mRNA levels and energy expenditure may be altered or controlled by various dietary patterns. Further research is needed to elucidate the effects of diet on the regulation of many obesity-related genes.

• Asian-Australasian Journal of Animal Sciences
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• 제32권2호
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• pp.170-175
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• 2019

Objective: Uncoupling protein 3 gene (UCP3) is a candidate gene associated with the meat quality of pigs. The aim of this study was to explore the regulation mechanism of UCP3 expression and provide a theoretical basis for the research of the function of porcine UCP3 gene in meat quality. Methods: Bisulfite sequencing polymerase chain reaction (PCR) and quantitative real-time PCR (Q-PCR) were used to analyze the methylation of UCP3 5′-flanking region and UCP3 mRNA expression in the adipose tissue or skeletal muscle of three pig breeds at different ages (1, 90, 210-day-old Putian Black pig; 90-day-old Duroc; and 90-day-old Dupu). Results: Results showed that two cytosine-guanine dinucleotide (CpG) islands are present in the promoter region of porcine UCP3 gene. The second CpG island located in the core promoter region contained 9 CpG sites. The methylation level of CpG island 2 was lower in the adipose tissue and skeletal muscle of 90-day-old Putian Black pigs compared with 1-day-old and 210-day-old Putian Black pigs, and the difference also existed in the skeletal muscle among the three 90-day-old pig breeds. Furthermore, the obvious changing difference of UCP3 mRNA expression was observed in the skeletal muscle of different groups. However, the difference of methylation status and expression level of UCP3 gene was not significant in the adipose tissue. Conclusion: Our data indicate that UCP3 mRNA expression level was associated with the methylation status of UCP3 promoter in the skeletal muscle of pigs.

• 사상체질의학회지
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• 제19권1호
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• pp.171-185
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• 2007

1. Objectvies This experimental study was designed to investigate the effect of cheongpesagan-tang extract on the obstruction of the lipase activity and weight, plasma, UCP1, 2 mRNA in db/db mouse. Material and Methods: The body weight loss, food intake, feeding efficiency ratio, weight of the internal organs (liver, kidney, epididymal fat, brown adipose tissue), plasma glucose, triglyceride, total cholesterol, white adipose tissue, adipocyte size distribution, expression of UCP1, 2 mRNA were measured in db/db mouse administered Cheongpesagan-tang extract for 6 weeks. These were then compared with those of control groups administered the diet. 2. Results 1) Inhibitory effect against lipase activity was Kilgyung(81.7%), Nabokja (73.1%), Seungma(73.0%), Daewhang (68.4%), Kalgeun (55.3%), Kobon(34.5%), Hwanggeum(4.2%). 2) In the sample group, the body weight was significantly decrease than that of control group. 3) In the sample group, the weight of epididymal fat showed significantly decrease than that of control group. 4) In the sample group, triglyceride showed significantly decrease than that of control group. 5) In the sample group, distribution of adipose tissue showed significantly larger than that of control group. 6) In the sample group, UCP1, 2 mRNA in BAT showed significantly increase than that of control group. 3. Conclusions These results show that cheongpesagan-tang has an effect on the treatment of obesity.

• 한국식품영양과학회지
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• 제31권5호
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• pp.788-795
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• 2002

본 연구는 프락토즈 중합체인 레반의 지질 감소 효과와 비만 유전자인 UCP를 통한 에너지 대사에 미치는 효과를 살펴보기 위하여 성장기 6주간 AIN-76A diet로 사육한 흰쥐에게 레반을 식이 섭취량의 1%, 2%로 5주간 경구투여하여, 혈중 지질 수준과 체지방 형성 및 혈중 인슬린, 렙틴 농도와 갈색 지방 조직, 백색지방 조직, 골격 근육, 시상하부에서 의 UCP mRNA 발현량 변화를 관찰하였다. 연구 결과를 종합해 보면 다음과 같다. 1) 레반군에서 혈청 중성지방과 총 콜레스테롤은 감소하고 HDL 콜레스테롤 수준은 영향을 미치지 않아 1%와 2% 레반 공급이 지질 대사를 개선시킬 수 있음을 보여주었다. 2) 1%와 2% 레반군에서 내장 지방 무게가 감소하여 적은 양의 레반 공급으로 체지방 축적이 억제될 수 있음을 보여주었다. 3) 갈색 지방 조직과 부고환 지방, 복막 지방 무게는 그룹간 차이가 나타나지 않았다. 4) 혈청 인슬린 농도는 1% 레반군에서, 혈청 렙틴 농도는 1% 레반군, 2% 레반군에서 대조군에 비해 감소하는 경향을 나타내었으나 통계적 유의성은 나타나지 않았다. 5) 1%와 2% 레반 공급에 의하여 갈색 지방 조직과 골격근육, 시상하부에서의 UCP mRNA발현량에는 변화가 없었다. 6) 백색 지방 조직의 UCP 2 mRNA 발현량이 레반 공급에 의해 증가하여 1%와 2%레반 공급에 의해 에너지 소비율이 증가할 수 있음이 나타났다 결론적으로 저농도의 1%와 1% 액상 레반 공급은 총 식이의 ,3%～5% 레반 공급과 비교하여 혈중 총 콜레스테롤과 중성 지방 감소 효과에는 차이가 있으나 UCP 발현 증가에 미치는 영향은 적은 것으로 보인다. 그러나 저농도의 1～2% 액상 레반 공급으로 지질 대사를 개선하고 체지방 축적을 어느 정도 억제하는 효과를 나타냄을 알 수 있으며 이에 관한 임상 연구로 레반의 고지혈증과 비만을 조절하는 기능성 식품 소재로서의 가능성 검색이 요망된다.

• 대한의생명과학회지
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• 제16권4호
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• pp.239-246
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• 2010

It is suggested that ovariectomy induces body weight gain primarily in the form of adipose tissue in rodents. Since liver peroxisome proliferator-activated receptor ${\alpha}$ (PPAR${\alpha}$) and uncoupling 2 (UCP2) are involved in the regulation of energy expenditure, it was investigated whether swim training regulates ovariectomy-induced adiposity and steatosis through liver PPAR${\alpha}$ and UCP2 activation in female ovariectomized mice, an animal model of postmenopausal women. Swim-trained mice had significantly decreased adipose tissue weights compared with sedentary control mice. Histological analysis showed that hepatic lipid accumulation was inhibited by swim training. Concomitantly, swim training significantly increased mRNA levels of PPAR${\alpha}$ and its target genes responsible for peroxisomal fatty acid ${\beta}$-oxidation, such as acyl-CoA oxidase, enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase and thiolase in the liver. Moreover, swim training induced the mRNA expression of UCP2. These results suggest that swim training can effectively prevent adiposity and steatosis caused by ovariectomy, in part through activation of liver PPAR${\alpha}$ and UCP2 in female obese mice.

• Journal of Acupuncture Research
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• 제22권3호
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• pp.77-92
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• 2005

가시오가피약침(五加皮藥鍼)이 대사증후군(代謝症候群)에 미치는 영향을 알아보기 위하여 ICR mouse에 고지방식이로 비만과 대사증후군(代謝症候群)을 유도하면서 신유(腎兪)(BL23)에 상응하는 부위에 일정한 방법으로 가시오가피약침(五加皮藥鍼)후 체중, 식이효율, 혈중 glucose, insulin level, insulin resistance, 경구내당능, 혈압, 혈중지질의 변화, 지방조직의 중량과 면적, 조직학적 변화와 GLUT-4 mRNA 및 UCP-1 mRNA의 발현을 관찰하여 유의한 결과를 얻었다. 1. 가시오가피약침(五加皮藥鍼)은 체중의 증가와 식이효율의 상승을 억제하였다. 2. 가시오가피약침(五加皮藥鍼)은 혈중 glucose, insulin level 및 insulin resistance의 상승을 억제하고, 경 구내당능을 개선시켰다. 3. 가시오가피약침(五加皮藥鍼)은 혈압의 상승을 억제하였다. 4. 가시오가피약침(五加皮藥鍼)은 혈중지질의 이상을 개선시켰다. 5. 가시오가피약침(五加皮藥鍼)은 WAT, BAT 및 liver 중량의 증가를 억제하였고, WAT와 BAT의 면적과 직경의 증가를 억제하였다. 6. 가시오가피약침(五加皮藥鍼)은 GLUT-4 mRNA 및 UCP-1 mRNA 발현의 증가를 억제하였다.

• 생명과학회지
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• 제19권1호
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• pp.15-20
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• 2009

Uncoupling protein 3 (UCP3)는 골격근에서 주로 발현되는 미토콘드리아 단백질이다. 생체의 대사기능에 관여할 것으로 알려져 있지만, 아직 그 역할에 대하여 깊이 연구되어 있지 않다. 최근에 고지혈증 치료제인 페노파이브레이트를 처치한 쥐의 간에서는 UCP3가 새로이 발현 유도된다는 것이 알려졌으며, 본 연구자들은 새로이 발현되는 UCP3가 비만쥐의 지방조직올 감소시키며, 인슐린 감수성을 증가시킨다는 보고를 하였다. 본 연구에서는 이러한 새로이 발현되는 UCP3의 시간에 따른 발현 양상과 대사 기능의 연관 관계를 규명하고자 하였다. 8주령 흰쥐를 두 군으로 나누어, 한 군은 고지방 식이를 실시하고(HF군), 다른 군은 고지방 식이와 페노파이브레이트(HFF군)를 함께 복용토록 하였다. 10주 동안 식이와 약물을 처리하면서, 1, 3, 6주에 간조직올 분석하였으며, 매주 먹이량, 체중, 체온을 측정하였다. 페노파이브레이트에 의하여 간에서 새로이 발현되는 UCP3는 2주부터 시작하여 3, 6주에 아주 높은 발현 양상을 보였다. 두 군 간의 먹이량의 차이는 없는 반면, 페노파이브레이트 처리군에서 6 주부터 시작하여 체중증가가 억제되며, 오히려 감소되는 경향을 보였다. 체온은 페노파이브레이트 처리군에서 6주부터 상승하기 시작하여, UCP3의 발현에 의한 간에서의 upcoupling 현상에 따른 발열현상으로 추측할 수 있다. 이상의 결과들로 유추해 볼 때, UCP3 발현이 3-6주부터 많은 양이 생성되어 생체의 대사 기능에 영향을 미치며, 이러한 시기와 연관되어 체중, 체온의 변화와 관련이 있는 것으로 생각한다.

• Journal of Microbiology and Biotechnology
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• 제14권4호
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• pp.796-804
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• 2004

We found previously that dietary high fat caused obesity, and levan supplementation to the regular diet reduced adiposity and serum lipids. In the present study, we examined the effects of levan [high-molecular-mass $\beta$-(2,6)-linked fructose polymer] supplement on the development of obesity and lipid metabolism in rats fed with high-fat diet. Thus, to determine whether the dietary levan may have the anti-obesity and hypolipidemic effects, 4-wk-old Sprague Dawley male rats were fed with high-fat diet for 6 wk to induce obesity, and subsequently fed with 0, 1, 5, or 10% levan supplemented high-fat diets (w/w) for another 4 wk. For the comparison, a normal control group was fed with AIN-76A diet. Supplementation with levan resulted in a significant reduction of high-fat-induced body weight gain, white fat (i.e., epididymal, visceral, and peritoneal fat) development, adipocyte hypertrophy, and the development of hyperinsulinemia and hyperlipidemia in a dose-dependent manner. Serum triglyceride and free fatty acid levels were greatly reduced by levan supplementation. Serum total cholesterol level was reduced, whereas the HDL cholesterol level was increased by dietary levan. The expression of uncoupling protein (UCP) was increased by dietary high fat, and was further induced by levan supplementation. The mRNA level of UCP1, 2, and 3 in brown adipose tissue (BAT) and UCP3 in skeletal muscle was upregulated in rats fed with dietary levan. In conclusion, upregulated UCP mRNA expression may contribute to suppression of development of obesity through increased energy expenditure. The present results suggest that levan supplementation to the diet is beneficial in suppressing diet-induced obesity and hyperlipidemia.