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Development of molecular markers for varietal identification of Brassica juncea on the basis of the polymorphic sequence of ITS regions and MITE families (갓 (Brassica juncea) 품종구분을 위한 ITS 영역 및 MITE Family 정보를 이용한 분자표지 개발)

  • Yang, Kiwoung;Yi, Go-eun;Robin, Arif Hasan Khan;Jeong, Namhee;Lee, Yong-Hyuk;Park, Jongin;Kim, Hoyteak;Chung, Mi-Young;Nou, Ill-Sup
    • Horticultural Science & Technology
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    • v.34 no.2
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    • pp.305-313
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    • 2016
  • Brassica juncea (2n = 4x = 36, AABB genome, 1,068 Mb) is a U's triangle species and an amphidiploid derivative of B. rapa and B. nigra. Fifteen varieties were used to study the ITS (internal transcribed spacer) regions of ribosomal DNA and MITEs (miniature inverted-repeat transposable elements) with a view of developing specific molecular markers. ITSs and MITEs are an excellent resource for developing DNA markers for genomics and evolutionary studies because most of them are stably inherited and present in high copy numbers. The ITS (ITS1 and ITS2) sequence was compared with the consensus sequence of B. rapa and B. nigra. Variation in ITS1 created two separate groups among 15 varieties, with 10 varieties in one group and 5 in the other. Phylogenetic analysis revealed two major clusters for those 10 and 5 varieties. Among the 160 different MITE primers used to evaluate the selected 15 varieties of B. juncea, 70 were related to the Stowaway, 79 to the Tourist, 6 to the hAT, and 5 to the Mutator super-families of MITEs. Of 160 markers examined, 32 were found to be polymorphic when fifteen different varieties of B. juncea were evaluated. The variety 'Blackgat' was different from the other mustard varieties with respect to both phenotype and genotype. The diversity of 47 additional accessions could be verified using eight selected molecular markers derived from MITE family sequences. The polymorphic markers identified in this study can be used for varietal classification, variety protection, and other breeding purposes.

Geochemical and Structural Geological Approach for clarifying Stratigraphy of Quartzite in the Paju Area: an Application of Rare Earth Element and Nd Isotope in Stratigraphy (파주지역 규암의 층서관계 규명을 위한 지구화학적-구조지질학적 연구: 층서규명을 위한 희토류원소 분포도와 Nd 동위원소의 응용)

  • Koh Hee Jae;Lee Seung-Gu;Lee Byung-Joo
    • The Journal of the Petrological Society of Korea
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    • v.14 no.2 s.40
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    • pp.116-126
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    • 2005
  • The Precambrian quartzite and calc-schist layers experienced multi-1310ing events are distributed along the two kinds of U-shaped 1310 (Fold I and II) with $N10^{\circ}E-directed$ fo1d axis in Wollong-myeon, Gwangtan-myeon, Jori-myeon of Paju city, the northeastern part of Gyeonggido. Occurrence of 10 layers of quartzite and 4 layers of calc-schist is not clear whether quartzite and schist layers were deposited sequentially each other or one to two layers of quartzite and schist were distributed repeatedly by isoclinal folding and thrusting, because of lack of sedimentary structures. In this paper, we tried to clarify the correlative relationship among the quartzite beds which are distributed along the U-shaped folds using geochemical tools such as rare earth element (REE) patterns and Nd isotope ratio. Quartzites have characteristics of LREE-flattened, HREE- slightly depleted patterns. They also show Ce negative anomaly whereas there are no Eu anomalies. As a result, quartzite beds occurred along the bilateral sides of fold axis show very similar REE patterns from outer side to inner side of 1314. The Nd model age of quartzite layers shows a trend that the inner part of fold is younger than the outer part of it. Such geochemical characteristics suggest that bilateral quartzite beds occurred along the fold axis were derived from the cogenetic source materials. The REE patterns and trace element geochemistry of mica schist intercalated within quartzite indicate that the quartzite and mica schist may be derived from different source materials. Our results suggest that REE and Nd isotope geochemistries may be very useful in clarifying the relationship of sedimentary deposits which do not show stratigraphical and structural connections in the field.

A Case Study of the Implementation Mechanism of Home Economics Curriculum in South Korea and the U.S. (한국과 미국의 가정과 교육과정 실행 과정 사례 연구)

  • Kim, Eun Jeung;Kwon, Yoojin;Lee, Yoon-Jung
    • Journal of Korean Home Economics Education Association
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    • v.27 no.3
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    • pp.79-97
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    • 2015
  • Curriculum may be defined as everything students learn at school or the specific plan of a subject that students are supposed to learn. The purpose and goals of the curriculum or who is in charge of developing the curriculum may differ by country. This study aimed at comparing the mechanisms in which the Home Economics curriculum is implemented in Korea and the Unite States at different levels. Considering that Korean government now supports increased autonomy of schools, allowing them to execute curricula according to their educational environments, it is meaningful that the advantages and disadvantages of the implementation mechanisms of two countries are explored. Specifically, middle schools in a City of Kyung-gi Province in South Korea and three states(Wisconsin, Ohio, and Washington states) in the United States are examined. The curriculum documents at different levels as well as study plans used by actual teachers are compared for this purpose. In case of South Korea, the nation state is in charge of curriculum development and the curriculum document defines the educational contents to detail in order to provide standardized learning experience to students. This type of closely controlled system warrants standardized educational contents and thus allows nation-wide assessment using standardized measures. On the other hand, it is difficult to accommodate diversities among students in terms of aptitudes and learning styles, and also limits creativity enhancement or integrative approach to teaching and learning. In comparison, curriculum in the United States is rather loosely defined, and more autonomy is granted to schools and teachers. Each of these two different systems may have advantages and disadvantages of their own. The findings of this study is expected to provide implications to curriculum development as well as to the development of framework and guidelines to the curriculum implementation.

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A Study on Goryo Celadons of intaglio '○' pattern and '⊙'pattern (고려 음각 '○'문과 '⊙'문청자의 연구)

  • Han, Seong-Uk
    • KOMUNHWA
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    • no.70
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    • pp.133-161
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    • 2007
  • It is judged that celadons with '○' pattern and '⊙' pattern have the indication of manufacturer by engraving mark at the internal center of the bottom. These celadons were produced at No.8.23.27 kiln sites located at Sadang-ri(사당리), Daegu-myon(대구면), Gangjin-gun(강진군), Jeollanamdo(전라남도), Korea and '⊙’ patterned celadon porcelain was found only at No. 23 and 27 kiln sites, so it is assumed that its production sites were limited. Kinds of celadons included bowl, dish, cup, bottle, case and vessel for emptied wine and there were a lot of dishes with a variety of patterns. These celadons were engraved by molded design technique using the same ceramic design as the pattern of celadons in the period of their prosperity and it was appropriate method to produce celadons of a good quality with production method to keep decorative design like pattern or size uniformly. It was also found that inlay method was not universalized in this period and similar celadons were produced with molded design in large quantities. These celadons had partially fire clay temper, but most of them were burnt by supporting the inner bottom of U-type base with quartzite after whole glazing. It was known through relics excavated at Seokreung(석릉) royal tomb of King Heejong(희종) at Ganghwa(강화) and Hyeeumwon(혜음원) temple site at Paju(파주) and military house at Wakamiyaoji(약궁대로) avenue Kamakura(겸창)-city, Japan(일본) that these celadons were manufactured at two quarters of the 13th century centering around 1230s. Therefore, these celadons will be significant materials to fill the gap of the celadons in the 13th century resulted from the record chronologically that green celadons were manufactured in the 12th century. And this period was invaded by Mongolia(몽고), but central control was achieved and green celadons were continuously produced. It was also considered that these celadons were excavated at Gabgot-ri(갑곶리) and Gwanchung-ri(관청리), Ganghwa(강화), Hyeeumwon temple, Paju which played the role of temporary palace, Bu1guksa(불국사) temple at Gyeongju(경주), Silsangsa(실상사) temple at Namwon(남원) and relics of Jangheung(장흥) adjacent to Daeguso(대구소) and these were produced for high class.

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The Limitation of Air Carriers' Cargo and Baggage Liability in International Aviation Law: With Reference to the U.S. Courts' Decisions (국제항공법상 화물.수하물에 대한 운송인의 책임상한제도 - 미국의 판례 분석을 중심으로 -)

  • Moon, Joon-Jo
    • The Korean Journal of Air & Space Law and Policy
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    • v.22 no.2
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    • pp.109-133
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    • 2007
  • The legal labyrinth through which we have just walked is one in which even a highly proficient lawyer could easily become lost. Warsaw Convention's original objective of uniformity of private international aviation liability law has been eroded as the world community ha attempted again to address perceived problems. Efforts to create simplicity and certainty of recovery actually may have created less of both. In any particular case, the issue of which international convention, intercarrier agreement or national law to apply will likely be inconsistent with other decisions. The law has evolved faster for some nations, and slower for others. Under the Warsaw Convention of 1929, strict liability is imposed on the air carrier for damage, loss, or destruction of cargo, luggage, or goods sustained either: (1) during carriage in air, which is comprised of the period during which cargo is 'in charge of the carrier (a) within an aerodrome, (b) on board the aircraft, or (c) in any place if the aircraft lands outside an aerodrome; or (2) as a result of delay. By 2007, 151 nations had ratified the original Warsaw Convention, 136 nations had ratified the Hague Protocol, 84 had ratified the Guadalajara Protocol, and 53 nations had ratified Montreal Protocol No.4, all of which have entered into force. In November 2003, the Montreal Convention of 1999 entered into force. Several airlines have embraced the Montreal Agreement or the IATA Intercarrier Agreements. Only seven nations had ratified the moribund Guatemala City Protocol. Meanwhile, the highly influential U.S. Second Circuit has rendered an opinion that no treaty on the subject was in force at all unless both affected nations had ratified the identical convention, leaving some cases to fall between the cracks into the arena of common law. Moreover, in the United States, a surface transportation movement prior or subsequent to the air movement may, depending upon the facts, be subject to Warsaw, or to common law. At present, International private air law regime can be described as a "situation of utter chaos" in which "even legal advisers and judges are confused." The net result of this barnacle-like layering of international and domestic rules, standards, agreements, and criteria in the elimination of legal simplicity and the substitution in its stead of complexity and commercial uncertainty, which manifestly can not inure to the efficient and economical flow of world trade. All this makes a strong case for universal ratification of the Montreal Convention, which will supersede the Warsaw Convention and its various reformulations. Now that the Montreal Convention has entered into force, the insurance community may press the airlines to embrace it, which in turn may encourage the world's governments to ratify it. Under the Montreal Convention, the common law defence is available to the carrier even when it was not the sole cause of the loss or damage, again making way for the application of comparative fault principle. Hopefully, the recent entry into force of the Montreal Convention of 1999 will re-establish the international legal uniformity the Warsaw Convention of 1929 sought to achieve, though far a transitional period at least, the courts of different nations will be applying different legal regimes.

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A Study on the Structure Style of Street Green Spaces on Port Island, Kobe, Japan (일본 고베시(神戶市) 포트아일랜드 가로녹지 구조 유형 연구)

  • Kwak, Jeong-In;Han, Bong-Ho;Noh, Tai-Hwan;Kwak, Nam-Hyun
    • Journal of the Korean Institute of Landscape Architecture
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    • v.43 no.4
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    • pp.62-74
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    • 2015
  • This study was carried out to provide examples of planting methods for the construction of street green spaces with abundant greenery by analyzing road type, surrounding land use and planting structures in street green spaces on Port Island, Kobe, Japan. Port Island_(total area: 826ha) is a marine cultural city located in Japan's first artificial island with facilities of urban function and port facilities. The study site was designated at 11 plots of $55{\sim}285m^2$ on Port Island, and topography structure styles were divided into four types with mounding style, slope style, slope and flat style, flat style according to the adjacent roads width. The area adjacent to the middle roads with high levels of noise and pollution set up the mounding style, slope style, slope and flat style of multi-layer structures using topographic properties. The area adjacent to small roads focused on a green strip with shrubs on a flat style. Surrounding land-uses include a public institution, housing complex, and a commercial building. The planting concept was a buffer and landspace function in case of the middle road_(lane 4) while the small road_(lane 2) was a landspace function. Planting species were diverse with Liquidambe formosana, Cinnamomum camphora, Sapium sebiferum, Cedrela sinensis, Laeocarpus sylvestris var. ellipticus, Ginkgo biloba, Prunus serrulata var. spontanea, Zelkova serrata, Quercus glauca, Juniperus chinensis, Magnolia kobus, Rhododendron spp., Camellia japonica, Abelia mosanensis, etc. Planting density was 0.02~0.08(0.04) individual/$m^2$ at the canopy layer, 0.02~0.08(0.04) individual/$m^2$ at the understory layer. Ratio of green coverage was 40.0~173.7(93.0)% at the canopy layer, 2.1~79.8(34.9)% at the understory layer and 17.9~64.2(32.9)% at the shrub layer. $Gr{\ddot{u}}volumenzahl$ was $1.43{\sim}6.67(4.13)m^3/m^2$ at the canopy layer, $0.02{\sim}2.01(0.85)m^3/m^2$ at the understory layer and $0.14{\sim}0.58(0.26)m^3/m^2$ at the shrub layer. The ratio of green coverage of street green space on Port Island was higher than that of Seoul, and particularly, the ratio of green coverage and $gr{\ddot{u}}volumenzahl$ at the shrub layer differed, compared to the main street green space in Korea. The result of this study may be applicable to other coastal reclaimed cities in terms of setting methods for street greenery considering the topography structure, planting structure and planting function.

The Risk Assessment of the Fire Occurrence According to Urban Facilities in Jinju-si (진주시 도시시설물별 화재발생 위험도 평가)

  • Bae, Gyu Han;Won, Tae Hong;Yoo, Hwan Hee
    • Journal of Korean Society for Geospatial Information Science
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    • v.24 no.1
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    • pp.43-50
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    • 2016
  • Urbanization in Korea has increased significantly and subsequently, various facilities have been concentrated in urban areas at high speed in accordance with a growing urban population. Accordingly, damages have occurred due to a variety of disasters. In particular, fire damage among the social disasters caused the most severe damage in urban areas along with traffic accidents. 44,432 cases of fire occurred in 2015 in Korea. Due to these accidents, 253 were killed and property damage of 4,50 billion won was generated. However, despite the efforts to reduce a variety of damage, fire danger still remains high. In this regard, this study collected fire data, generated from 2007 to 2014 through the Jinju Fire Department and the National Fire Data System(NFDS) and calculated fire risk by analyzing the clustering of fire cases and facilities in Jinju-si based on the current DB of facilities, offered by the Ministry of Government Administration and Home Affairs. As a result, the risk ratings of fire occurrence were classified as four stages under the standards of the US Society of Fire Protection Engineers(SEPE). Business facilities, entertainment facilities, and automobile facilities were classified as the highest A grade, detached houses, Apartment houses, education facilities, sales facilities, accommodation, set of facilities, medical facilities, industrial facilities, and life service facilities were classified as U grade, and other facilities were classified as EU grade. Finally, hazardous production facilities were classified as BEU grade, the lowest grade. In addition, in the case of setting the standard with loss of life, the highest risk facility was the hazardous production facilities, while in the case of setting the standard with property damage, a set of facilities and industrial facilities showed the highest risk. In this regard, this study is expected to be effectively utilized to establish the fire reduction measures against facilities, distributed in urban space by calculating risk grades regarding the generation frequency, casualties, and property damage, through the classification of fire, occurred in the city, according to the facilities.

Effects of Balance Taping Therapy on Improving Knee Pain and the Obstacle in Daily Activity in Rural Elderly Women (밸런스 테이핑요법이 농촌 여성노인의 무릎통증과 일상활동장애 개선에 미치는 영향)

  • Kim, Ae-Jung;Park, Hae-Sook
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.7
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    • pp.218-227
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    • 2019
  • This study examined the effects of balance taping therapy on improving knee pain and the obstacles to daily activity in rural elderly women. The research design was a quasi-experimental research using a non-equivalent control group pre-test and post-test design. The data were collected from January 19th to April 14th, 2017. The research subjects included female elderly over 65 years old who visited 12 senior centers located at Y city in rural areas. The participants were composed of 26 people in the experimental group and 28 people in the control group. Data were collected before and 24 hours after balance taping therapy, and the pain and obstacles to daily activity were measured. The collected data were analyzed using the PASW Statistics 23 Program, which included a $X^2-test$, Shapiro-Wilk test, and Mann-Whitney U test. After the intervention, significant differences were observed in the knee pain (Z=-6.658, p<.001) and obstacles to daily activity (Z=-3.466, p=.001). With regard to lower domain variables of the obstacles to daily activity, significant differences in standing up (Z=-2.860, p=.004), daily activity (Z=-2.629, p=.009), walking (Z=-3.868, p<.001), and dressing up (Z=-2.049, p=.040) were observed between the two groups. On the other hand, there were no significant differences in grip (Z=-.542, p=.588) and arm stretching (Z=-.416, p=.678). In conclusion, applying balance taping to rural elderly women clearly decreases the level of knee pain, reduces the obstacles to daily activity, and improves their quality of life.

Causes of High PM2.5 Concentrations in Cheongju Owing to Non-Asian Dust Events (비황사 사례에 기인한 청주시 PM2.5 고농도 원인)

  • Kim, Da-Bin;Moon, Yun-Seob
    • Journal of the Korean earth science society
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    • v.41 no.6
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    • pp.557-574
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    • 2020
  • The purpose of this study is to analyze the cause of high PM2.5 mass concentrations in Cheongju for the period of non-Asian dust days using the weather chart, the stream lines at 850 hPa, the backward trajectory, and the weather and air quality model. As a result of analyzing the time series of PM2.5 concentrations and weather charts for the episodic days in Cheongju, the weather patterns were shown in related to long-range transport of PM2.5 from China or surrounding areas. In fact, in the PM2.5 time series, 60-80 ㎍ m-3, which is more than 2-3 times higher than the concentration attributed to Cheongju activities, was observed as a background concentration related to long-range transport. The distribution of high PM2.5 concentration was typically dependent on the locations of the high and low pressures above the ground while the upper jet stream passed through the Korean Peninsula. Consequently, the high PM2.5 concentration in Cheongju is due to massive air pollutants in the form of smog originated from industrial, household and energy combustion sources of Beijing and other nearby regions of China. These air pollutants move along a fast zonal wind caused by the atmospheric pressure arrangement. high concentration of PM2.5 in Cheongju City is because the mass of air pollutants in the form of smog generated from industrial, household and energy combustion origins in Beijing or other nearby regions of China move along a fast wind speed zone according to the atmospheric pressure arrangement of long-distance transportation. Air pollutants including PM2.5 show an M-shaped pattern that passes through the topography of the Cheongju basin from north to south as a belt or band-shaped pollutant. The ground high pressure according to the above-ground high pressure expansion area and cut-off low or low pressure arrangement, or the bands in the form of river stems appear in a gradual incremental pattern that changes into a U-shape under the influence of the wind.

Study on Manufacturing Techniques and Conservation for Earthenware Horn Cups with a Horse Head Decoration(Treasure) (보물 도기 말머리장식 뿔잔의 제작 기법 연구와 보존처리)

  • KWON, Ohyoung;HAM, Chulhee;YU, Jia;KIM, Hanseul;PARK, Changyuel
    • Korean Journal of Heritage: History & Science
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    • v.55 no.1
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    • pp.51-61
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    • 2022
  • Earthenware horn cups with horse head decorations were excavated from Tomb No. 7 of Bokcheon-dong, Dongraegu, Busan Metropolitan City. Made of earth in the shape of a horn, these cups are considered to have been used to drink alcohol or beverage. Large numbers of earthenware horn cups of various shapes were excavated from tombs located in the old territories of Silla and Gaya. A pair of earthenware horn cups were excavated from Tomb No. 7, and the two cups are almost identical in overall shapes and manufacturing techniques despite different sizes. Conservation treatment was carried out for the bigger one of the two horn cups this time. There are two cracks toward the horse head decorations around the mouth with missing parts observed. The chest of the horse touches the ground with one side decorating the horse head and the other side facing the conical mouth of the horn cup. It is in the U shape, striking a balance based on two legs attached behind. The surface of the horn cup was made with a potter's wheel, and the connection to the horse head has traces of cutting and trimming. The horse head is expressed realistically with its features including the ears, eyes, nose, and mouth well apprehended and its color is grey This study intended to investigate manufacturing techniques of the artifact by examining its internal structure through the condition survey in a non-destructive way. CT imaging was used to figure out its manufacturing techniques and to diagnose its condition, and accordingly the scientific conservation treatment was conducted to stabilize the artifact. The precise diagnosis on conservation condition found that there are two chips in the spout with their cracks extended. One of the chips is connected with separation added to the crack. The material which has been used for connection in the past was collected for the infrared spectroscopic analysis, which was identified to be nitrocellulose resin for the connection. Therefore, this conservation treatment focused on removing the old material and preventing the spread of cracks. Before conservation treatment, the condition survey and scientific examination for the artifact were carried out to secure data about the earthenware horn cup with horse head decorations(Treasure). Based on them, effective plans for its conservation treatment was sought for and then existing adhesive was safely removed, and restoration material was selected to take into account its reversibility. In addition, the conservation treatment according to optimal methodologies was conducted through the consultation meeting with experts.