• 한방안이비인후피부과학회지
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• 제22권3호
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• pp.36-46
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• 2009

Objective : Recently, the demands for the effective and safe depigmentating and anti-aging agents of the skin have increased due to the medical, pharmaceutical and cosmetic reasons. The aim of this study is to search new materials from the traditional herbal medicines which inhibit the aging process of skin in vitro. Methods : herbal medicines(80% ethanol or water extracts) were screened for their inhibitory activities against elastase. Results : Rheum undulatum (final concentrstion 1 mg/ml) appeared over 30% of inhibition of elastase activity. so we are investigated anti wrinkle effects of Rheum undulatum look through MMP-1 inhibition activity. also Extracts of Rheum undulatum showed higher anti tyrosinase activity than arbutin (final concentration 1 mg/ml). Conclusion : These results suggest that herbal medicines could be strong potential sources of inhibition of anti-aging and whitening effects for the skin.

• 생약학회지
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• 제46권1호
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• pp.1-5
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• 2015

In the course of screening tyrosinase inhibitory activity, EtOAc-soluble fraction of Nelumbinis Semen (Seeds of Nelumbo nucifera) showed significant inhibition. Further fractionation of the EtOAc-soluble fraction resulted in 12 compounds, which were identified as 4-(hydroxymethyl)phenol (1), tyrosol (2), 4-(hydroxymethyl)benzaldehyde (3), 4-hydroxybenzoic acid (4), 4-(2-methoxyvinyl)benzene-1,2-diol (5), 2,6-dihydroxybenzoic acid (6), (2R-trans)-2,3-dihydro-3,5,7,8-tetrahydroxy-2-(4-hydroxyphenyl)-4H-1-benzopyran-4-one (7), (+)-catechin (8), elephantorrhizol (9), (+)-dehydrovomifoliol (10), (-)-boscialin (11) and uridine (12). Compounds 5 and 7 were first reported from this plant. Among the isolated compounds, compound 7 showed strong inhibition on tyrosinase activity with mixed mechanism of competitive and noncompetitive inhibition.

• 약학회지
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• 제56권6호
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• pp.395-400
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• 2012

To investigate the possibility of development as a whitening agent using arctigenin, we measured DPPH assay, NBT/XO assay, intracellular ROS scavenging assay, tyrosinase assay and MSH-induced melanin production in B16 melanoma cells. Arctigenin dose-dependently had anti-oxidant activity in DPPH, NBT/XO and intracellular ROS assay. Although arctigenin did not inhibit purified tyrosinase activity, it dose-dependently inhibited tyrosinase activity and melanin production in B16 melanoma cells stimulated by $1{\mu}M$ ${\alpha}$-MSH. In particular, arctigenin at a concentration $100{\mu}M$ inhibited ${\alpha}$-MSH-stimulated tyrosinase activity and melanin production by $50.9{\pm}2.9%$ and $69.0{\pm}6.5%$ respectively. And typical tyrosinase inhibitor, arbutin, inhibited $57.7{\pm}2.9%$ and $65.1{\pm}5.0%$ respectively. Such an similar inhibitory effect of arctigenin and arbutin in B16 melanoma cells may be due to the inhibition of MSH signal pathway rather than the direct inhibition of tyrosinase. Therefore, these results suggest that arctigenin may be useful for the development as whitening agents.

• 한국식품영양과학회지
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• 제41권2호
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• pp.156-160
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• 2012

본 연구에서는 복숭아 유과 과육의 품종에 따른 총 폴리페놀 함량과 항산화능 및 tyrosinase 저해 활성을 알아보고자 하였다. 품종별 복숭아 유과 과육의 총 폴리페놀 함량은 7.24~9.95 mg/g의 함량을 나타내며 마도카에서 가장 낮고 몽부사에서 가장 높았으며, DPPH 및 ABTS radical 소거능에서 몽부사의 radical 소거능이 가장 높게 나타났다. Reducing power에서의 항산화 실험 결과 역시 몽부사에서 가장 높게 나타났으며, tyrosinase 저해 활성에서는 홍백에서 가장 높은 tyrosinase 저해능을 보였으나 6품종간의 유의적 차이는 확인할 수 없었다. 따라서 본 연구 결과에 따라 복숭아 유과과육은 항노화 및 미백활성으로 화장품 산업에서 천연 물질로 잠재적인 기능성을 가지고 있을 것으로 사료된다.

• 생약학회지
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• 제32권3호통권126호
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• pp.175-180
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• 2001

Solvent extracts of 17 plants collected in Cheju Island were investigated for their biological properties related to cosmeceuticals such as tyrosinase and hyaluronidase inhibition and also radical scavenging effects. The chloroform fraction of Phytolacca esculenta root exhibited strong inhibition against tyrosinase activity. No fraction showed significant hyaluronidase inhibition. Some solvent extracts of plants such as Achyranthes japonica and Artemisia princeps showed considerable radical scavenging activities.

• Biomolecules & Therapeutics
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• 제13권1호
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• pp.32-34
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• 2005

Effect of isoliquiritigenin isolated from the heartwood of Dalbergia odorifera T. Chen (Leguminosae) on mushroom tyrosinase activity was investigated in vitro using L-tyrosine and L-3, 4-dihydroxyphenylalanine (L-DOPA) as the substrates. When L-tyrosine was used as a substrate, both isoliquiritigenin and kojic acid, a positive control, inhibited tyrosinase activity in a concentration-dependent manner. IC$_{50}$ values of isoliquiritigenin and kojic acid were 61.4 and 52.2 ${\muM}$, respectively. However, isoliquiritigenin showed week inhibitory effect on the oxidation of L-DOPA by tyrosinase with inhibition ratio of 9.1 ${\pm}$ 7.1% at 100 ${\muM}$. It is also suggested that 3-unsubstituted and 4-hydroxyl phenyl group in isoliquiritigenin plays an important role on the inhibition of tyrosinase activity when L-tyrosine was used as a substrate. Analysis of Lineweaver-Burk plot showed that isoliquiritigenin acts as a competitive inhibitor in case of L-tyrosine as a substrate.

• 한방안이비인후피부과학회지
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• 제20권3호
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• pp.51-62
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• 2007

Objective : The aim of this study is to assess the effect of Schizandrae Fructus on melanin synthesis inhibition and whitening effect. Methods : We assessed inhibitory effects of Schizandrae Fructus on melanin-release from B16F10, on melanin production in B16F10, on mushroom tyrosinase activity in vitro, on tyrosinase activity in B16F10 and effect of Schizandrae Fructus on the expression tyrosinase, TRP-1, TRP-2, PKA, ERK-1 ERK-2, AKT-1, MITF in B16F10. Results and Conclusion : 1. Schizandrae Fructus inhibited melanin-release, melanin production in B16F10. 2. Schizandrae Fructus inhibited tyrosinase activity in vitro and in B16F10. 3. Schizandrae Fructus suppressed the expression of tyrosinase, TRP-1, TRP-2, PKA, ERK-2 in B16F10.

• 한국자원식물학회지
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• 제14권1호
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• pp.32-37
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• 2001

한약재 174종으로부터 tyrosinase 저해제를 탐색하였다. 한약재는 30일간 methanol에 담가 놓은 후, 그 추출액을 제조하여 사용하였다 Tyrosinase의 활성을 측정하기 위해서는 효소 mushroom Tyrosinase와 기질 L-DOPA, phosphate buffer를 사용하였다. 탐색 결과, 한약재 174종 중 14개종이 tyrosinase를 저해 효과를 보였고, 그중 오배자 추출액이 inhibition activity가 가장 높게 나타났다.

• 한방안이비인후피부과학회지
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• 제23권1호
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• pp.135-148
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• 2010

Recently, the demands for the effective and safe depigmentating and anti-aging agents of the skin have increased due to the medical, pharmaceutical and cosmetic reasons. The aim of this study is to search Galla Rhois (GR) inhibitory activity against aging process of skin in vitro. GR was screened for their inhibitory activity against elastase. GR (final concentration 1 mg/ml) appeared over 40% of inhibition of elastase activity. So we investigated anti wrinkle effects of GR look through MMP-1 inhibition activity. Also Extracts of GR showed higher anti-tyrosinase activity than arbutin (final concentration 1 mg/ml). These results suggest that GR could be potential sources of anti-aging and whitening effects for the skin. Objective : Galla Rhois extracts, Elastase (pancreatic from porcine pancreas), N-Succinyl-(Ala)3-p-nitroanilide, Ursolic acid, mushroom tyrosinase, L-tyrosine, arbutin, Amicon Ultra, Hand Hold UV lamp, MMP-1 primary antibody, MMP-1 secondary antibody Result : 1. GR appeared over 40% of elastase inhibitory activity. 2. GR had MMP-1 inhibition activity. 3. GR showed higher anti-tyrosinase activity than arbutin. Conclusion : According to above results, it is supposed that Galla Rhois could be strong potential sources of anti-aging and whitening effects for the skin.

• 한국식품저장유통학회지
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• 제9권4호
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• pp.385-390
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• 2002

마이크로웨이브 추출(MAE)을 이용하여 만가닥버섯을 물, 50% 에탄올, 99% 에탄올로 추출하였으며 마이크로웨이브의 power와 시간에 따라 추출물의 총 폴리페놀 함량, 전자공여 작용(EDA), tyrosinase 저해작용, 아질산염 소거작용의 차이를 관찰하였다. Microwave power를 60∼120 W로 증가시킴에 따라 총 폴리페놀 함량과 전자공여능은 증가하는 경향을 나타내었다. 만가닥버섯의 전자공여능이 90 W에서 평형에 이른 반면에 tyrosinase 저해능은 90 W 이상에서 급격히 증가하였다. 50% 에탄을 추출물과 99% 에탄을 추출물의 아질산염 소거능은 microwave power를 증가시킴에 따라 확연히 증가하는 경향을 나타내었으며 pH 1.2에서 99% 에탄을 추출물은 물 추출물에 비해 약 5배 정도의 높은 값을 나타내었다. 추출시간에 따른 총 폴리페놀 함량을 살펴본 결과 물 추출물의 경우 추출시간 5분에서 평형에 이르렀다. 전자공 여능은 5∼10분 사이에 급격히 증가하다가 10분에서 평형에 이르렀으며 물 추출물에서 가장 높게 나타내었다. tyrosinase 저해능은 microwave power 증가에 따라 점진적으로 증가하는 경향을 나타내었다.