Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Screeing of Tyrosinase Inhibitors from Oriental Herbs

한약재로부터 Tyrosinase 저해제의 탐색

  • 서승염 (공주대학교 생물학과 및 (주)약용자원콜렉션)
  • Published : 2001.03.01
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Abstract

Mammalian tyrosinase plays an important role in the process of melanin polymer biosynthesis by catalyzing the hydroxylation of tyrosine to 3,4-dihydroxyphenylalanine(DOPA) and the oxidation of DOPA to dopaquinone. These processes are major determinant of human skin color and involved in localized hyperpigmentation. Therefore, the enzyme inhibitors have been of great concern as skin-whitening cosmetics. Methanol extracts of 174 oriental herbs were screened for the mushroom tyrosinase inhibitory activity.

한약재 174종으로부터 tyrosinase 저해제를 탐색하였다. 한약재는 30일간 methanol에 담가 놓은 후, 그 추출액을 제조하여 사용하였다 Tyrosinase의 활성을 측정하기 위해서는 효소 mushroom Tyrosinase와 기질 L-DOPA, phosphate buffer를 사용하였다. 탐색 결과, 한약재 174종 중 14개종이 tyrosinase를 저해 효과를 보였고, 그중 오배자 추출액이 inhibition activity가 가장 높게 나타났다.

Keywords

References

  1. Pigment Cell Res. v.13 no.SUP.8 The melanocortin-1 receptor is a key regulator of human cutaneous pigmentation Abdel-Malek Z.;Scott M.C.;Suzuki I.;Tada A.;Im S.;Lamoreux L.;Ito S.;Barsh G.;Hearing V.J.
  2. Nippon Hifuka Gakkai Zasshi v.101 Inhibitory effect of arbutin on melanogenesis-biochemical study using cultured B16 melanoma cells Akiu S.;Suzuki Y.;Asahara T.;Fujinuma Y.;Fukuda M.
  3. Biochem Pharmacol v.57 Inhibitors of mammalian melanocyte tyrosinase : in vitro comparisons of alkyl esters of gentisic acid with other putative inhbitors Curto E.V.;Kwong C.;Hermersdorfer H.;Glatt H.;Snatis C.;Virador V.;Hearing V.J.Jr.;Dooley T.P.
  4. Immunol v.135 Immunochemical analysis of the modulation of human melanoma-associated antigens by DNA recombinant immune interferon Giacomini P.L.;Imberti L.;Aguzzi A.;Fisher P.B.;Trinchieri G.;Ferrone S.
  5. Pigment Cell Res. v.13 Melanosome capping of keratinocytes in pigmented reconstructed epidermis-effect of ultraviolet radiation and 3-isobutyl-1-methyl-xanthine on melanogenesis Gibbs S.;Murli S.;De Boer G.;Mulder A.;Mommaas A.M.;Penec M.
  6. J. Am. Osteopath. Assoc. v.73 The effect of ascorbic acid and potassium ferricyanide as melanogenesis inhibitors on the development of pigmentation in Mexican axolotls Haas G.E.
  7. Int. J. Biochem. v.19 Mammalian tyrosinase: The critical regulatory control point in melanocyte pigmentation Hearing V.J.;Jimen M.
  8. Pigment Cell Res. v.13 no.SUP.8 Chemical analysis of melanins and its application to the study of the regulation of melanogenesis Ito S.;Wakamatsu K.;Ozeki H.
  9. J. Invest. Dermatol. v.75 Dopachrome conversion: A possible control point in melanin biosynthesis Korner A.M.; Pawelek J.
  10. Exp Eye Res. v.69 Increased pigmentation of iridial melanocytes in promates induced by a prostaglandin analogue Lindquist N.G.;Larsson B.S.;Stjernschantz J.
  11. 香粧會誌 v.19 Evaluation of antimelanogenic effects Matsukami M.
  12. Proc Natl. Acad. Sci. U.S.A. v.79 [half-Cys4, half-Cys10]-alpha-Melanocyte-stimulating hormone: a cyclic alpha-melanotropin exhibiting superagonist biological activity Sawyer T.K.;Hruby V.J.;Darman P.S.;Hadley M.E.
  13. Tohoku J. Exp. Med. v.149 Stimulation of melanogenesis by chlecalciferol in cultured human melanocytes Tomita Y.;Fukushima M.;Tagama H.