• Title/Summary/Keyword: Two-rowed

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Nucleic Acid Degrading Enzymes of Barley Malt (맥아의 핵산분해효소)

  • Lee, Won-Jong
    • Korean Journal of Food Science and Technology
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    • v.21 no.1
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    • pp.1-8
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    • 1989
  • Ten cultivars of malting barley grown at four locations were malted and assayed for six enzymes involved in the degradation of nucleic acids. Among these enzymes were deoxyrinonuclease, ribonuclease, phosphodiesterase, 3'- and 5'- nucleotidases and phosphomonesterase. Activities of all enzymes in five-day malts were significantly affected by variety and location of growth. The average levels of ribonuclease, deoxyribonuclease, 3'-nucleotidase and 5'-nucleotidase of 80 five-day malts were 11.2, 5.7, 5.6 and 1.2 units per gram of malt, respectively. Six-rowed barley malts contained higher levels of deoxyribonuclease, phosphodiesterase and 3'-nucleotidase than those of two-rowed barley malts, while two-rowed barley malts contained significantly higher ribonuclease levels than those of six-rowed barley malts.

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Activities of Hydrolytic Enzymes in Barley Malts Prepared under Different Germination Conditions (발아조건별 당화용 엿기름의 분해효소 활성도)

  • Mok, Chul-Kyoon;Lee, Young-Tack
    • Applied Biological Chemistry
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    • v.42 no.4
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    • pp.324-329
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    • 1999
  • Barley malts were prepared at 15, 18 and $21^{\circ}C$ for $3{\sim}6$ days, and assayed for ${\beta}-glucanase$, ${\alpha}-amylase$ and ${\beta}-amylase$ activities. ${\beta}-Glucanase$ activity increased markedly during earley germination and reached maximum at the 6th day of germination. ${\beta}-Glucanase$ activity in six-rowed barley malt was much higher than that in two-rowed malt. ${\beta}-Glucanase$ activity was associated with reduction in ${\beta}-glucan$ content during germination. ${\beta}-amylase$ activity was also considerably higher in two-rowed barley, and increased continuously during 6-day germination. ${\beta}-Amylase$ activity was the lowest at $15^{\circ}C$, the highest at $18^{\circ}C$, and intermediate at $21^{\circ}C$ of germination temperature. Considerable amount of ${\beta}-amylase$ was detected in ungerminated raw barley, and this enzymatic activity tended to increase during 6-day germination. Diastatic power, measure of starch-saccharifying enzyme, in six-rowed malt was $1.4{\sim}1.6$ fold higher than in two-rowed malt. Germination at $18^{\circ}C$ for $5{\sim}6$ days was suggested to be the optimum condition for manufacturing good quality malts, in terms of enhanced starch-degrading enzymatic activity.

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Fungal Distribution and Varieties Resistance to Kernel Discoloration in Korean Two-rowed Barley (국내 육성 2조 겉보리 변색 종실에서의 곰팡이 분포와 품종 저항성)

  • Shin, Sang-Hyun;Seo, Eun-Jo;Choi, Jae-Seong;Kang, Chun-Sik;Lee, JungKwan;Park, Jong-Chul
    • Research in Plant Disease
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    • v.19 no.3
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    • pp.183-187
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    • 2013
  • Barley kernel discoloration (KD) leads to substantial loss in value through downgrading and discounting of malting barley. The objective of this research is to investigate fungal distribution and varieties resistance to KD in Korean two-rowed barley. Several fungal organisms including Alternaria spp., Fusarium spp., Aspergillus spp., Epicoccum spp. and Rhizopus spp. were isolated from Korean two-rowed barley representing KD. The symptoms of KD were brown and black discolorations of the lemma and palea. The most frequently detected fungal species was Alternaria spp. which exhibited 69.1% and 72.2% in 2011 and 2012, respectively. Epicoccum spp., Fusarium spp., and Aspergillus spp. were also detected. Fusarium spp., primary pathogen of barley head blight, were rarely occurred in the 2011 and their occurrence increased to 4.7% in 2012. Twenty cultivars of Korean two-rowed barely were evaluated to KD. The average percentage of KD was 8.0-36.0% in 2011 and 5.2-36.6% in 2012. Two cultivars ('Sacheon 6' and 'Dajinbori') showed KD of 6.2% to 8.8% and determined resistant, however 'Samdobori' and 'Daeyeongbori' demonstrating KD of 22.2-36.6% were highly susceptible. 'Jinyangbori', 'Danwonbori', 'Sinhobori' and 'Kwangmaegbori' showing KD of less than 15% were moderately resistant cultivar.

Analysis of a 6th Industrialization Model in the Saemangeum Grain Complex (새만금 복합곡물단지의 6차산업화 모델 분석)

  • Kim, Yooan;Jung, Chanhoon;Kim, Solhee;Kim, Chanwoo;Suh, Kyo
    • Journal of Korean Society of Rural Planning
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    • v.25 no.2
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    • pp.63-74
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    • 2019
  • As the awareness of food security has increased, the Korean government has established national projects, such as Saemangeum proclaimed land, to secure sources of grain. Saemangeum is a large-scale agricultural area that was constructed to maintain preparedness for unstable food markets. This study aims to develop a $6^{th}$ Industrialization Model (SIM) for Saemangeum Grain Complex by applying feasible strategies to wheat and two-rowed barley which have low self-sufficiency rates. In addition, this study estimates the potential economic value of each development strategy associated with a sixth industrialization model to create higher added values from production, processing and tourism experiences. The strategic plan for primary, secondary, and tertiary industries is to combine cultivating and processing wheat and two-rowed barley for sales and linking them to tourism experience. This study shows value added from the combination of the primary, secondary and tertiary industry of wheat and two-rowed barley are 7.5 and 23.0 times more than those of the primary and tertiary industry combination, respectively. Through branding Saemangeum Grain Complex's products, such as Saemanguem bread and craft beer, would further enhance the economic benefits derived from the complex.

Effects of Ozone and Light Illumination on the Enzymation Activity of Malt (오존 및 광선의 처리가 맥아 효소활성에 미치는 영향)

  • 오만진;김혜정;이정호;최성현;권병구
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.28 no.1
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    • pp.67-73
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    • 1999
  • In order to increase the enzymatic activity of malt used as a source of traditional processing foods, the enzymatic activities of various barley were examined and the effects of ozone and light illumination treatment on the enzymatic activities of amylase, amylase, and glucanase in malt during man ufacture were also examined. Barley didn't show amylase activity prior to soaking, but the activity of barley increased quickly after soaking. Glutinous barley showed the highest amylase activity among Duru barley, Ol barley, two rowed barley and naked barley. Naked barley showed the lowest activity. The amylase activity was the highest in Duru barley and decreased in the order of in glutinous barley, naked barley and two rowed barley. It was showed that the enzymatic activity of malt was higher than that of control when malt was soaked for 24hr at the concentration of 0.3ppm of ozone. The enzymatic activity of malt treated with light illumination was higher than that of control. The bud and root of light illuminated malt was much stronger than that of control. The root of light illuminated malt was much shorter than that of control. In addition, light illuminated malt showed a little green color which matches the demand of consumer. These studies demonstrated that both ozone and light illumination treatment increased the enzymatic activity of malt to result in high quality of malt manufacture.

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A new species of Corydalis (Fumariaceae): C. bonghwaensis M. Kim & H. Jo (현호색속(현호색과)의 신종: 봉화현호색(Corydalis bonghwaensis M. Kim & H. Jo))

  • Jo, Hyun;Shin, Changkeun;Kim, Muyeol
    • Korean Journal of Plant Taxonomy
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    • v.47 no.4
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    • pp.308-315
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    • 2017
  • A new species, Corydalis bonghwaensis M. Kim & H. Jo, is described here. It is found on a mountain slope in Bongwha-gun, Gyeongsangbuk-do, Korea. Corydalis bonghwaensis shares several characteristics (white flowers, glabrous pedicels, white tubers, and the polygonal stigma) with related species C. namdoensis B. U. Oh & J. G. Kim and C. albipetala B. U. Oh. However, this new species has flowers which change from pale-yellow to white, a mucronated inner-petal apex, linear leaflets, and fusiform capsules with two-rowed seeds. In contrast, C. namdoensis has retuse inner-petal apex, various leaflets, and fusiform capsules with 2-rowed seeds. C. albipetala has retuse inner-petal apex, elliptical leaflets, and linear capsule with 1-rowed seeds. Also C. humilis B. U. Oh & Y. S. Kim has pale-blue purple flowers, elliptical leaflets, inflorescence with 1-5 flowers, and hemispherical lower-outer petal.

Strain Distinction and Their Distribution of Barley Yellow Mosaic Virus Base on RAPD Analysis in Korea

  • Park, Jong-Chul;Rho, Tae-Whan;Kim, Jung-Gon;Kim, Hyung-Moo;So, In-Young;Lee, Kui-Jae
    • Korean Journal of Plant Resources
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    • v.20 no.6
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    • pp.511-517
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    • 2007
  • A stable method for strain distinction using viral RNA 1 structures analyses was developed and compared with the combined RT-PCR and RAPD methods. Seven out of 61 random primers were found to be polymorphic based on RAPD analysis resulting on the differentiation of the 33 BaYMV isolates into four distinct groups according to geographical districts. The first and largest group includes 13 isolate and consists mainly of two-rowed malting barley in Haenam area. The second group had ten collections from inland in west southern. The third group had seven isolates from west southern coastal region, where mainly six-rowed naked barley is cultivated. The last fourth group included three isolates from Gyungnam region in east southern area. Conclusively, RNA 1 analysis proved to be stable and efficient method for strain distinction for Korean BaYMV isolates. Further, results of pathogenicity and RNA 1 structure analyses revealed four groups BaYMV strains and were distributed all over Korea, represented by Naju, Haenam-okcheon, Iksan and Milyang.

Genotypic Variation of Esterase Isozyme in Breeding Lines of Two-rowed Barley by Electrophoretic Banding Pattern (전기영동 패턴에 의한 2조보리 계통의 Esterase 동위효소 유전자형 변이분석)

  • 박광근;최홍집;이종호;서세정;김재철;남중현;김상효
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.47 no.6
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    • pp.465-470
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    • 2002
  • This study presents results of electro-phoretically detectable isozyme variation in Crossing Block (CB) lines of two-rowed barley maintained by the National Crop Experiment Station. The specific objectives were to determine allelic frequencies at the four Est loci(Est1, Est2, Est4, and Est5) and their distribution over 380CB lines of two-rowed barley. A total of 17 alleles were detected over the four Est loci in these lines. There were 4 alleles (Pr, Al, Ca, and Af at the Est1 locus and their frequencies were 69.7, 1.1, 28.4, and 0.8%, respectively. At the Est2 locus, 5 different alleles (Dr, Fr, Sp, Un and a recessive null allele) were detected and their frequencies were 2.9,84.5,0.5,2.1, and 10%, respectively. four alleles (Nz, Su, At, and null were detected at the Est4 locus and the allelic frequency of Su was about 84%. Four alleles(Mi, Pi, Te, and a null allele(od)) were detected at the Est5 locus and their frequencies were 34.2, 61.0, 2.4, and 2.4%, respectively. Based on the allelic frequencies over the four Est loci, 380 CB lines were classified into 25 genotypes. The most frequent genotypes were G1(Pr-Fr-Su-Mi) and G2(Pr-Fr-Su-Pi), and their frequencies were 28.1 and 39.5%, respectively. The frequencies of other genotypes were less than 10%.

Changes in the Free, Bound and Neutral Lipids of Malt during Malting (맥아제조시 유리.결합 및 중성 지질의 변화)

  • Shin, Seung-Lyeul;Song, Jun-Hee;Kim, Kwang-Soo;Park, Jyung-Rewng;Lee, Kap-Rang
    • Applied Biological Chemistry
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    • v.29 no.4
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    • pp.346-351
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    • 1986
  • This study was carried out to investigate the changes in bound, free, and neutral lipid components of malt during malting, two-rowed barley. During malting, the temperature and relative humidity were $17^{\circ}C$ and 80%, respectively. The content of free lipids in both two-rowed barley and their malt was much higher than that of bound lipids. Decrease in the content of free lipids during malting was more prominent than that of bound lipids. The content of neutral lipids was 21.0mg/g-d. w. out of 27.9mg/g-d. w. of total lipids extracted from two-rowed barley. The content of neutral lipids decreased during malting. Triglyceride, free fatty acid and sterol ester were the principal components of neutral lipids. The content of triglyceride decreased during malting, but the content of free fatty acid and sterol ester increased. Linoleic, palmitic, oleic and linolenic acid were the principal fatty acid of free and bound lipids. The content of palmitic acid in free lipids increased during malting, but that of bound lipids decreased. The content of oleic acid in free lipids decreased. The principal fatty acids of neutral lipids were similar to those of free lipids. The content of palmitic acid increased during malting, but that of linoleic and stearic acid decreased.

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Anther Culture Efficiency According to Plating Method in Naked Barley (쌀보리 약배양을 위한 약치상 방법별 배양효율)

  • Park, Tae-Il;Jeoung, Sun-Ok;Kim, Young-Jin;Kim, Hyun-Soon;Seo, Jae-Hwan;Park, Ki-Hun;Kim, Jung-Gon;Yun, Song-Joong
    • Journal of Plant Biotechnology
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    • v.34 no.3
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    • pp.237-242
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    • 2007
  • Barley anther culture is hard working to plating picking out anther from the glume and demand long time comparing to be short available development stage for effective culture. Also, it has been treatment massive materials due to low plantlet comparing to get desirable plants intensively. Consequently, this experiment was carried out trying to be more high barley anther culture effectively in terms of save plating effort. Plating materials and culture temperature affected anther culture efficiency are among the inoculation tissues or organs such as anthers, spikelets and whole panicles, culture efficiency was higher with spikelets in two-rowed than six-rowed barley due primarily to a lower contamination, and calli were induced within 30 to 50 days. Callus induction and plant regeneration rates were higher in cultures at $25^{\circ}C$ than at $15^{\circ}C$ and $20^{\circ}C$. Days to callus induction were 25 to 50 days at $25^{\circ}C$ and 50 to 60 days at $20^{\circ}C$.