• BMB Reports
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• v.41 no.10
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• pp.739-744
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• 2008

Turnip yellow mosaic virus (TYMV) is a positive strand RNA virus that infects mainly Cruciferae plants. In this study, the TYMV genome was modified by inserting an extra subgenomic RNA promoter and a multiple cloning site. This modified TYMV was introduced into Nicotiana benthamiana using a Agrobacterium-mediated T-DNA transfer system (agroinfiltration). When a gene encoding $\beta$-glucuronidase or green fluorescent protein was expressed using this modified TYMV as a vector, replication of the recombinant viruses, especially the virus containing $\beta$-glucuronidase gene, was severely inhibited. The suppression of replication was reduced by co-expression of viral silencing suppressor genes, such as tombusviral p19, closteroviral p21 or potyviral HC-Pro. As expected, two subgenomic RNAs were produced from the recombinant TYMV, where the larger one contained the foreign gene. An RNase protection assay revealed that the recombinant subgenomic RNA was encapsidated as efficiently as the genuine subgenomic RNA.

• BMB Reports
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• v.41 no.11
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• pp.778-783
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• 2008

Turnip yellow mosaic virus (TYMV) RNA has two hairpins in its 5' untranslated region (5'-UTR). To investigate the role of the hairpins in replication of TYMV, mutants lacking one or both of the two hairpins were constructed. The TYMV constructs were introduced into Chinese cabbage by an Agrobacterium-mediated T-DNA transfer method, called agroinfiltration. Analysis of total RNA from agroinfiltrated leaves showed that replication of the mutant TYMV RNA lacking both hairpins was about 1/100 of wild type. This mutant was also impaired in systemic spread. Deletion analysis of each hairpin revealed that both hairpins were needed for maximal replication. The deletion analysis along with sequence modification of the hairpin structure indicates that the second hairpin plays a role in efficient long-distance systemic movement of TYMV.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2004.10a
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• pp.68-69
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• 2004

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• Proceedings of the Korean Society of Plant Pathology Conference
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• 2005.04a
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• pp.5-6
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• 2005

• 한국균학회소식:학술대회논문집
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• 2015.05a
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• pp.50-50
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• 2015

Both agriculture and local tourism of Kagoshima prefecture where is located on the south-western region of the Japanese mainland, are the important industries. Although cabbage (Brassica oleracea) has been cultivated in recent decades in Kagoshima, clubroot disease caused by Plasmodiophora brassicae had never been observed. However, the disease in cabbage was reported in four regions last couple years. Our survey showed that one region is infested severely whereas others are slightly. In the most widely infested region, the disease was also observed in turnip rape (Brassica rapa) which is grown as ornamental plants for landscape design in early spring and important tourist attraction. Consequently, both agriculture and local tourism are damaged by clubroot. The increase of clubroot incidence in this region might be caused by significant increase of cabbage production, the expansion of cropping season throughout the year and continuous turnip rape cultivation in the same fields of cabbage for almost three decades. Therefore we are trying to estimate the risk of clubroot damage cultivation throughout the year in this region. We collected five isolates of resting spores and identified them as race 3, 4 and 9 by Williams' method, and as pathotype group 3 and 4 by classification system using clubroot resistant (CR) $F_1$ cultivars of Chinese cabbage as differential hosts as described in Hatakeyama et al.(2004). Furthermore, we found that these populations were avirulent to commercial CR cabbages. These results indicate that introduction of CR cabbage and breeding of turnip rape are the effective measures to solve our problem.

• Journal of Plant Biotechnology
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• v.43 no.2
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• pp.248-254
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• 2016

The purpose of this study was to investigate biological activities of Brassica rapa (Turnip) plant callus extracts of Ganghwa-gun of Incheon city using water, ultrasonic wave and ethanol extractions to develop functional materials. DPPH radical scavenging activities of the callus extracts were increased in a concentration-dependent manner, as compared with control. The astringent effects of the ethanol extracts were higher, as compared to water and ultrasonic extracts. In the collagen synthesis assay, the ethanol extract showed significant anti-wrinkle effects of 59% and 78% at a concentration of 5 ppm and 10 ppm, respectively. These results suggested that water, ultrasonic wave and ethanol extracts of turnip plant calluses are natural antioxidant sources. Especially, the ethanol extract can be regarded as a functional, natural cosmetic material with astringent and anti-wrinkle effects.

• Korean Journal Plant Pathology
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• v.13 no.4
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• pp.248-254
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• 1997

Expression vector (pGEX-Tu) for the coat protein (CP) gene of turnip mosaic virus Ca strain (TuMV-Ca) was constructed by incorporation of TuMV CP gene into pGEX-KG vector which had ${\beta}$-galactosidase gene and IPTG (isopropylthio-${\beta}$-D-galactoside) induction site. The results of ELISA and western hybridization indicated that optimal condition of the expression were when IPTG and western hybridization indicated that optimal condition of the expression were when IPTG induction was carried out on YTA medium with ampicillin in 2 hours after the E. coli seed inoculation ($A_{595}$=0.1/ml). TuMV CP gene was expressed with GST (Glutathion S-Transferase) gene fusion system, and the size of fusion protein was estimated to be 59kDa, for TuMV CP was 33 kDa and GST was 26 kDa.

• Korean Journal of Medicinal Crop Science
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• v.14 no.1
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• pp.45-48
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• 2006

Alcohol dehydrogenase (ADH) and Aldehyde dehydroganase (ALDH) activities of turnip extracts with ultrasonification at $60,\;100^{\circ}C$ temperature were investigated. Ultrasonification extracts showed a increase as compared with the normal extracts. ALDH activities were high than ADH activities. So, turnip extracts was shown efficient plants in alcohol oxidation. Appraisers liked powder extracts better than liquid extracts in sensory score.

• The Plant Pathology Journal
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• v.21 no.4
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• pp.369-376
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• 2005

Turnip mosaic virus (TuMV) is an infectious viral pathogen on the cruciferous crops, predominantly Chinese cabbage (Brassica campestris subsp. pekinensis) and radish (Raphanus sativus). On the basis of the symptom development in selective differential hosts from indicator host species, Chinese cabbage and Korean radish inbred lines, the representative eight isolates of TuMV were divided into two major groups/or six types. Group I includes Th 1, Ca-ad7, and Cj-ca2-1 isolates, while group II includes the other isolates (rg-pfl, r 9-10, Rhcql-2, Stock and Mustard). According to the molecular phylogenetic analysis, these isolates, however, divided into two groups and two independent isolates. Phylogenetic analysis indicated that four isolates (Tu 1, r9-10, Stock and Rh-cql-2) formed a distinct phylogenetic group, and the other two isolates (Ca-ad7 and Cj-ca2-1) also formed another group. Mustard and rg-pfl isolates did not seem to have any relationship with these two groups. Taken together, these results indicated that virulence differentiation on host plants, molecular phylogenetic analysis of the nucleotide and the deduced amino acid of TuMV coat proteins did not show any relationship. The multi-resistant lines, Wonyae 20026 and BP058 in Chinese cabbage represent valuable genetic materials that can be used for crucifer breeding programs on TuMV resistance, but not in Korean radish.

• Journal of The Korean Society of Grassland and Forage Science
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• v.10 no.2
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• pp.115-120
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• 1990

A series of experiments were carried out for two successive years to investigate the productivity of forage turnip in mid-northern area of Korea. Fresh matter and dry matter yields of 6 foreign cultivars (Purple Top Strap Leaved, Purple Top White Globed, Seven Top, Shogoin, White Egg and Amber Globe) seeded either in early spring or in autumn after harvesting forage corn were evaluated with different seeding date and harvesting date as well as with different level of fertilization. The results obtained are summarized as follows: 1. The earlier the seeding date in spring (Mar. 29, Apr. 12, Apr. 16) was, the higher was the yield of fresh matter and dry matter as the plant growth ceased after mid June, when the temperature began to rise. 2. In spite of its high DM yield, the Japanese cultivar, Shogoin, showed poor forage quality for summer feeding due to its earlier bolting in harvesting season. 3. Of the 6 cultivars Purple Top White Globed showed highest DM yield (800 kg/ 10a) in mid area including SEOUL irrespective of planting season. 4. Although the turnips showed mostly positive yield responses to fertization, the differences were not great especially above the level of$N-P_2O_5-K_2O$: 5-3-4 kg/lOa. 5. IN TAEGWALLYONG, a northern area of Korea, the average fresh matter yield of the six cultivars tested amounted to 3,500 kg/lOa when drilled on June 30 and harvested on Aug. 30, although bulb formation during the summer was relatively poor. 6. Concluded, forage turnip is regarded to be a suitable catch-crop which has the potential to maintain and increase the total forage production in mid-northern area of Korea. The intercropping is recommendable especially for autumn planting well past the time forage corn has been harvested.