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http://dx.doi.org/10.5010/JPB.2016.43.2.248

Biological activities of Brassica rapa (Turnip) callus extracts by plant cell culture technology  

Shin, Su Young (Division of Ecological Conservation, National Institute of Ecology (NIE))
Moh, Sang Hyun (Anti-aging Research Institute of BIO-FD&C Co.,Ltd.)
Hwang, You Jin (Department of Biomedical Engineering, College of Health Science, Gachon University)
Publication Information
Journal of Plant Biotechnology / v.43, no.2, 2016 , pp. 248-254 More about this Journal
Abstract
The purpose of this study was to investigate biological activities of Brassica rapa (Turnip) plant callus extracts of Ganghwa-gun of Incheon city using water, ultrasonic wave and ethanol extractions to develop functional materials. DPPH radical scavenging activities of the callus extracts were increased in a concentration-dependent manner, as compared with control. The astringent effects of the ethanol extracts were higher, as compared to water and ultrasonic extracts. In the collagen synthesis assay, the ethanol extract showed significant anti-wrinkle effects of 59% and 78% at a concentration of 5 ppm and 10 ppm, respectively. These results suggested that water, ultrasonic wave and ethanol extracts of turnip plant calluses are natural antioxidant sources. Especially, the ethanol extract can be regarded as a functional, natural cosmetic material with astringent and anti-wrinkle effects.
Keywords
Anti-aging; Antioxidant; Sulforaphane; Cosmetic materials; Biotechnology;
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Times Cited By KSCI : 17  (Citation Analysis)
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1 Kim JS, Lee JY, Park KT, An BJ, Lee SH, Cho YJ (2013) The Biological activity from Prunella vulgaris extracts. Korean. J Food Preserv 20:234-241   DOI
2 Kim DH, Park TS, Son JH (2015) Anti-wrinkle activities verification of Buplerum falcatum extracts on CCD-986sk. J Appl Biol Chem 58:183-187   DOI
3 Ko SH, Kim YS, Lee JH, Kim IH, Kim SB, Roh KB, Shin SW, Jung ES, Park DH (2013) Functional characterization of callus extracts of apple 'Hirosaki' for cosmetic materials. KSMM Journal 28:244-248
4 Kwon ST, Kliebenstein DJ (2014) Response of turnip to Botrytis cinerea infection and their relationship with glucosinolate profiles. Korean J Plant Res 27:371-379   DOI
5 Lee JT, Jeong YS, An BJ (2002) Physiological activity of Salicornia herbacea and Its application for Cosmetic materials. Kor. J. Herbology 17:51-60
6 Lee JY, An BJ (2012) Whitening and Anti-wrinkling Effects of Fractions from Prunus persica Flos. Korean J. Microbiol. Biotechnol 40:364-370   DOI
7 Lee NN, Choi YE, Moon HK(2015) Somatic embryo induction and plant regeneration from cold-stored embryogenic callus of K. septemlobus. J Plant Biotechnol 42:388-395   DOI
8 Moure A, Cruz J. M, Franco D, Dominguez J. M, Sineiro J, Dominguez H, Nunez M. J, Parajo J. C (2001) Natural antioxidants from residual source. Food Chem 72:145-171   DOI
9 Oh ST, Jung HS, Cho MJ, Song MY, Moh SH, Seo HH, (2014) Effect of Artemisia annua Linne callus induced by plant cell culture technology on wound healing. Journal of the Korea Academia-Industrial cooperation society 15:5628-5636   DOI
10 Park YK, Kim HM, Park MW, Kim SR, Choi IW (1999) physicochemical and functional properties of turnip. J Korean Soc Food Sci Nutr 28:333-341
11 Potapovich AI, Lulli D, Fidanza P, Kostyuk VA, De Luca C, Pastore S, Korkina LG (2011) Plant polyphenols differentially modulate inflammatory responses of human keratinocytes by interfering with actiation of transcription factors $NF{\kappa}B$ and AhR and EGFR-ERK pathway. Toxicol Appl Pharmacol 255:138-149   DOI
12 Yamani MI (1993) Fermentation of brined tunrnip roots using Lactobacillus plantarum and Leuconostoc mesenteroides starter cultures. World Journal of Microbiology and Biotechnology 9:176-179   DOI
13 Bang MH, Lee DY, Han MW, Chng HG, Jeong TS, Choi MS, Lee KT, Baek NI (2009) Isolation and identification of secondary metabolites from the roots of Brassica rapa J. Plant Biotechnol 36:64-67   DOI
14 Choi HC, Oh CH, (1997) Evaluation of skin furrows in the ageing process using an image analysis system. Kor. J Dermatol 35:292-302
15 Choi HJ, Han MJ, Baek NI, Kim DH, Jung HG, Kim NJ (2006) Hepatoprotective effects of Brassica rapa (turnip) on d-galactosamine induced liver injured rats. Kor J Pharmacogn 37:258-265
16 Chiao JW, Chung FL, Kancherla R, Ahmed T, Mittelman A, Conaway CC (2002) Sulforaphane and its metabolite mediate growth arrest and apoptosis in human prostate cancer cells. Int J Oncol 20:631-636
17 Fotakis G, Timbrell JA (2006) In vitro cytotoxicity assays: comparison of LDH, neutral red, MTT and protein assay in hepatoma cell lines following exposure to cadmuium chloride. Toxicol Lett 160:171-177   DOI
18 Huang D, Ou B, Prior RL (2005) The chemistry behind antioxidant capacity assays. J Agric Food Chem 53:1841-1856   DOI
19 Ha JO, Ha TM, Lee JJ, Kim AR, Lee MY (2009) Chemical components and physiological functionalities of Brassica campestris ssp rapa Sprouts. J Korean Soc Food Sci Nur 38:1302-1309   DOI
20 Ha JH, Han JG, Jeong HS, Oh SH, Kwon MC, Choi YB, Ko JR, Lee HY (2008) Enhancement of Immune Activities of Natural Water-Soluble Sulforaphane by Nano Encapsulation Process. Korean J. Medicinal Crop Sci 16:402-408
21 Jeon YS, Jo BS, Park HY, Kang SA, Cho YJ (2012) Screening of biological activity of Caragana sinica extracts. J Korean Soc, Food Sci. Nutr 41:1211-1219   DOI
22 Jo BS, Cho YJ (2012) Biological activity of extracts from Acanthopanax sessiliflorum fruit. Korean J Food Preserv 19:586-593   DOI
23 Kim MR, Lee KJ, Kim JH, Sok DE (1997) Determination of sulforaphane in cruciferous vegetables by SIM. Korean J. Food Sci Technol 29:882-887
24 Kim JS, Choi YH, Seo JH, Lee JW, Kim YS, Ryu SY, Kang JS, Kim YK, Kim SH (2004) Chemical constituents from the root of Brassica campestris ssp rapa. Kor J Pharmacogn 35:259-263
25 Kim JY, Lee YH, Kim JY, Roh BK, (2005) Study of antioxidation action of Lenonuri herba extract. J Soc Cosmet Scientists Korea 31:189-196
26 Kim HH, Cho S, Lee S, Kim KH, Cho KH, Eun HC (2006) Photoprotective and anti-skin-aging effects of eicosapentaenoic acid in human skin in vivo. J Lipid Res 47:921-930   DOI
27 Kim HR, Lee JH, Kim YS, Kim KM (2007) Chemical characteristics and enzyme activities of inchon ge-geol radish, ganghwa turnip and korean radish. Korean J Food Sci Technol 39: 255-259
28 Kim JY, Park SH, Lee KT (2009) Sulforaphane content and antioxidative effect of cooked broccoli. J East Asian Soc Dietary Life 19:564-569