• Tuberculosis and Respiratory Diseases
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• 제55권6호
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• pp.597-611
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• 2003

연구배경 : 다양한 암종들에서 빈번히 발견되는 염색체 16번 단완의 돌연변이가 소세포폐암의 발생 및 진행과 관련하여 어떤 양상을 보이는지 알아보기 위해 우리나라 소세포폐암 환자들을 대상으로 종양억제유 전자좌를 찾고자 하였다. 대상 및 방법 : 연세대학교 의과대학 세브란스병원에서 원발성 소세포폐암으로 진단된 23명의 남자 환자를 대상으로 하였다. 암 조직과 이에 대응하는 정상 조직의 파라핀 포매 블록으로부터 DNA를 추출하였으며, 염색체 16번 단완에 위치하는 20개의 현미부수체 표지자들을 이용하여 PCR-LOH 분석을 시행하였다. 결 과 : 1) 23예 중에서 LOH가 1개라도 관찰된 경우는 6예로 26.1%이었다(Fig. 1) 2) LOH가 있는 6예 중에서 비교적 넓은 부위의 결손이 관찰된 경우는 2예(SCLC1, SCLC2)로써 전체의 8.7%이었다(Fig. 1). 3) 검사를 시행한 20개의 표지자들중 50% 이상의 LOH 빈도를 보인 경우는 없었으나 다른 부위와 비교하여 우월하게 빈번한 빈도를 보인 부위는 D16S3024와 D16S748 사이에서 18.2%, D16S405에서 143%, D16S668과 D16S749 사이에서 21.1%, 그리고 D16S420과 D16S753에서 각각 8.3%의 빈도로 관찰되었다(Table 1, Fig. 1, Fig. 2) 4) Shifted bands는 23예 중 6예에서 관찰되었는데 a1tered loci의 빈도는 5~35% 이었다(Fig. 1, Fig. 2). 5) Shifted bands는 검사한 총 460개 loci중 3.3% 인 15 loci에서 관찰되었다(Fig. 1). 결 론 : 본 연구 결과 LOH 빈도가 낮은 문제점이 있으나 염색체 16번의 단완에는 원발성 소세포폐암 일부에서 종양의 발생 및 진행에 관여하는 최소 5개의 종양억제유전자좌가 존재할 가능성이 있는 것으로 추정되며, 향후 특이 유전자를 찾기 위한 추가적인 노력이 있어야 할 것으로 생각된다.

• 대한지역사회영양학회지
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• 제14권4호
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• pp.451-461
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• 2009

The purpose of this study was to investigate the age-related changes of cardiovascular disease risk factors and inflammatory markers in non-obese Korean women. Subjects were 112 women over 20 years old with body mass index (BMI) less than $30 kg/m^2$ and were divided into 3 groups (< 40 years, $40{\sim}59$ years, ${\ge}60$ years). Mean weight and BMI in the oldest group were significantly higher than those in the other 2 younger groups (p < 0.05). Mean total cholesterol, triglyceride, LDL-cholesterol and apolipoprotein B/apolipoprotein A1 ratio (BAR) in the oldest group were significantly higher than those in the youngest group (p < 0.05), and mean HDL-cholesterol of the oldest group was significantly lower than that of the youngest group (p < 0.05). The older-aged group showed significantly higher mean values of atherogenic index (AI) and LDL/HDL ratio (p < 0.05) than the respective younger-aged group, and AI was significantly correlated with age, nitric oxide and thiobarbituric acid reactive substances (p < 0.01). In addition, mean vascular cell adhesion molecule-l (VCAM-1) tended to be higher in the older-aged group than the younger group. Tumor necrosis factor-${\alpha}$, a proinflammatory maker, was significantly positively correlated with serum homocysteine, a cardiovascular disease risk factor (p < 0.01). In addition, a significantly positive correlation was observed between C-reactive protein and BAR (p < 0.01). Overall results suggested that the aging might affect the increase of cardiovascular disease risk factors including the serum lipid profiles, weight and BMI, and age-related increases of weight and BMI might play a role in changes in certain biomarkers of inflammation. (Korean J Community Nutrition 14(4) : 451${\sim}$461, 2009)

• Tuberculosis and Respiratory Diseases
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• 제69권1호
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• pp.48-51
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• 2010

Sarcoidosis is a multi-systemic granulomatous disorder of unknown etiology. The characteristic pathological finding is the presence of non-caseating granulomas. The lungs are primarily affected, however other organs may be involved causing various symptoms and ambiguous laboratory findings can be present. There are a few reported cases of sarcoidosis with elevated tumor markers. We describe a 68-year-old woman presenting with sarcoidosis showing elevated serum carcinoembryonic antigen (CEA). The possibility of cancer arising from serum CEA such as gastrointestinal cancer, breast cancer and lung cancer was excluded. A transbronchial lung biopsy demonstrated a non-caseating granuloma without necrosis. As a result prescribed 30 mg prednisolone daily to the patient and serum CEA was decreased after 1 month of treatment. We report a case of pulmonary sarcoidosis with elevated serum CEA.

• Asian Pacific Journal of Cancer Prevention
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• 제16권8호
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• pp.3111-3116
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• 2015

Background: This study was designed to investigate the value of CEA and CA199 in predicting the treatment response to palliative chemotherapy for advanced gastric cancer. Materials and Methods: We studied 189 patients with advanced gastric cancer who received first-line chemotherapy, measured the serum CEA and CA199 levels, used RECIST1.1 as the gold standard and analyzed the value of CEA and CA199 levels changes in predicting the treatment efficacy of chemotherapy. Results: Among the 189 patients, 80 and 94 cases had increases of baseline CEA (${\geq}5ng/ml$) and CA199 levels (${\geq}27U/ml$), respectively. After two cycles of chemotherapy, 42.9% patients showed partial remission, 33.3% stable disease, and 23.8% progressive disease. The area under the ROC curve (AUC) for CEA and CA199 reduction in predicting effective chemotherapy were 0.828 (95%CI 0.740-0.916) and 0.897 (95%CI 0.832-0.961). The AUCs for CEA and CA199 increase in predicting progression after chemotherapy were 0.923 (95%CI 0.865-0.980) and 0.896 (95%CI 0.834-0.959), respectively. Patients who exhibited a CEA decline ${\geq}24%$ and a CA199 decline ${\geq}29%$ had significantly longer PFS (log rank p=0.001, p<0.001). With the exception of patients who presented with abnormal levels after chemotherapy, changes of CEA and CA199 levels had limited value for evaluating the chemotherapy efficacy in patients with normal baseline tumor markers. Conclusions: Changes in serum CEA and CA199 levels can accurately predict the efficacy of first-line chemotherapy in advanced gastric cancer. Patients with levels decreasing beyond the optimal critical values after chemotherapy have longer PFS.

• Asian Pacific Journal of Cancer Prevention
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• 제16권4호
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• pp.1327-1330
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• 2015

Background: Squamous cell carcinoma (SCC) is the most common cancer in the oral area. Matrix metalloproteinases (MMPs) and especially MMP-2 and MMP-9 are increased in malignancy and lymph node involvement in oral SCCs. We aimed to evaluate the serum levels of MMP-2 and MMP-9 in patients with oral SCC compared to normal subjects and their relation with clinicopathological findings. Materials and Methods: In this case control study, 20 patients with oral SCC and 20 healthy subjects were included and serum levels of MMP-2 and MMP-9 were compared between groups. Also, the correlation between these markers with clinicopathological findings including grade (T) and node (N) were evaluated. Results: Patients with oral SCC had significantly higher serum levels of MMP-2 (p=0.01) and MMP-9 (p<0.001) compared to healthy subjects. With increase in grade T, MMP-2 was significantly increased (p=0.001), but in the MMP-9 case this was not significant (p=0.27). The levels of MMP-2 (p=0.002) and MMP-9 (p=0.01) in cases with lymph node involvement and that of MMP-2 in subjects with smoking history (p=0.001) were significantly high. There was significantly positive correlation between MMP-2 with grade T tumor (r=0.598, p=0.005), lymph node involvement (r=0.737, p<0.001) and smoking (r=0.674, p=0.001) and also between MMP-9 and lymph node involvement (r=0.474, p=0.03). Conclusions: Both markers are significantly increased in oral SCC compared to healthy subjects. However, MMP-2 was better for evaluating lymph node involvement and tumor grade.

• Asian Pacific Journal of Cancer Prevention
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• 제13권10호
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• pp.5155-5159
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• 2012

Objective: To investigate the association between CD105 and tumor cell proliferation in salivary gland tumors. Methods: In this study, 59 samples of salivary tumors from Khalili Hospital archive, including 20 cases of pleomorphic adenoma (PA), 20 cases of mucoepidermoid carcinoma (MEC) and 19 cases of adenoid cystic carcinoma, as well as 10 cases of normal salivary gland tissue, were reviewed by immunohistochemistry (IHC) for CD105 and Ki67 staining. Results: CD105 positive vessels were absent in normal salivary gland tissue in the vicinity of tumors (51.6% of all tumors were positive). There was a statistically significant difference in frequency of CD105 staining between PA and malignant tumors and between four groups of different lesions (p<0.000) being highest in MEC. Intratumoral microvessel density was also elevated in malignant neoplasms ($2.61{\pm}3.1$) as compared to PA ($0.46{\pm}0.6$). Normal salivary glands did not express Ki67. There was a statistically significant difference in frequency and percentage of Ki67 immunoreactivity in malignant neoplasms (86.5% and $10.7{\pm}10.8$ respectively) compared to PA (50% and $0.78{\pm}0.2$) and among the four groups values were highest in MEC (p<0.000). Conclusion: n this study, it was observed a higher rate of angiogenesis and cellular proliferation was noted in malignant tumors compared to benign tumors, but no correlation was observed between these two markers.

• 기술혁신학회지
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• 제15권2호
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• pp.369-399
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• 2012

목적: 본 연구는 보건의료분야 연구주제 중 암유전자 분야의 지식지도를 구축하고 국내외 연구주제 네트워크를 시계열적으로 비교 분석함에 의해 국내 연구동향을 파악하고 향후 암유전자 분야에서 활발하게 연구될 영역을 확인하고자 하였다. 방법: 자료는 PubMed 데이터베이스의 Medical Subject Headings(MeSH) 검색기능을 이용하여 'Oncogene'을 주제로 하는 논문을 추출하였고, 교신저자 소속이 한국인인 논문을 분리한 후 추출된 논문의 키워드를 대상으로 하였다. 분석방법은 사회네트워크 분석 기법 중 가중치가 부여된 연결정도 중심성을 적용하여 연구주제 네트워크를 구축하였으며, 이를 시기별로 나누어 연구주제 변화를 확인하였다. 결과: 암유전자의 경우 국내외 모두 'Genes, ras'와 'Apoptosis', 'Signal Transdction' 등이 연결정도 중심성이 가장 높은 키워드였으며, 시기별로는 국내외 모두 'antineoplastic Agents', 'Prognosis', 'Tumor Markers, Biological' 등 암유전자를 대상으로 치료 및 진단, 예후와 관련된 연구가 활발해졌다. 결론: 암유전자 연구주제 네트워크 분석 결과 시기별로 연구주제에 대한 일정한 흐름이 있었다. 따라서 다양한 연구주제 네트워크 분석으로 각 질환별 연구단계가 확인된다면 다음 단계의 연구진행을 예측할 수 있을 것이며 그에 따른 연구개발 지원을 위한 전략을 수립할 수 있을 것이다.

• 대한임상검사과학회지
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• 제37권3호
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• pp.220-227
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• 2005

Recently, the use of an electrical apparatus has brought up concerns of health risks from exposure to electromagnetic fields. EMF is composed of electric fields and magnetic fields. Heavy exposure to EMF can occur only in the vicinity of high-voltage overhead transmission lines, close to transformers and underground cables, and also close to large electrical machinery. In this thesis I have investigated the hypothesis of the correlation between occupational exposure to ELF-EMF and the risks of leukemia, anemia, cancer. Therefore, the aim of this study is to investigate whether or not ELF-EMF emitted from electric power stations and transformer substations affect some hematological parameters and tumor markers of electric utility workers. The hematological test results and tumor markers under investigation were similar in the two groups but some of parameters such as RBC, AFP, LDH showed significant difference between the two groups from two sample t-test (p<0.05). The exposure group showed increased LDH level compared to the control group by two sample t-tests. In addition, the abnormal LDH level in the exposure group was observed to be clinically significant by ${\chi}^2$-test. However, the levels of RBC, AFP observed were not clinically significant by ${\chi}^2$-test (p>0.05). These results suggested that ELF-EMF does not affect most blood test parameters except LDH of electric utility workers.

• 대한화장품학회지
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• 제48권3호
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• pp.265-273
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• 2022

본 연구에서는 한국산 겨우살이 (Viscum album L. var. coloratum)이 아토피 피부염과 관련된 염증성 사이토카인에 영향을 미치는지 여부를 알아보았다. 실험에는 헥산, 부탄올, 에틸아세테이트, 메틸렌클로라이드, 총 4 가지 분획물을 사용하였으며, RAW264.7 마우스 대식세포와 RBL-2H3 렛트 호중구를 이용하여 염증성 마커를 연구하였다. 실험 결과 에틸아세테이트 분획이 tumor necrosis factor alpha (TNF-α), interleukin(IL)-6, IL-4의 mRNA 발현 및 단백질 분비량을 감소시켰으나, 헥산 분획은 뚜렷한 효능이 없었다. 또한 부탄올 분획은 IL-4, IL-6의 mRNA 발현을 감소시켰고, 메틸렌클로라이드 분획은 IL-4와 TNF-α의 mRNA 발현을 감소시켰다. 결과적으로 한국산 겨우살이(V. album var. coloratum)가 아토피 피부염과 관련된 사이토카인 분비를 억제시켜 항염증 효과를 나타낼 수 있으므로, 이와 관련된 기능성 화장품 개발이 가능할 것으로 사료된다.

• Anatomy and Cell Biology
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• 제56권1호
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• pp.109-121
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• 2023

Thioacetamide (TAA) exposure and hepatitis C virus infection are usually associated with renal dysfunction. Sofosbuvir (SFV) and daclatasvir (DAC) drugs combination has great value in the treatment of hepatitis C. The study aimed to identify the nephrotoxic effects of TAA and to evaluate the ameliorative role of SFV and DAC in this condition. Forty-eight adult male albino rats were divided into eight groups and received saline (control), SFV, DAC, SFV+DAC, TAA, TAA+SFV, TAA+DAC and TAA+SFV+DAC for eight weeks. Kidney and blood samples were retrieved and processed for histological (Hematoxylin and Eosin and Masson's trichrome), immunohistochemical (α-smooth muscle actin), and biochemical analysis (urea, creatinine, total protein, albumin, malondialdehyde, reduced glutathione, superoxide dismutase, and tumor necrosis factor-α). Examination revealed marked destruction of renal tubules on exposure to TAA with either hypertrophy or atrophy of glomeruli, increase in collagen deposition, and wide expression of α-smooth muscle actin. Also, significant disturbance in kidney functions, oxidative stress markers, and tumor necrosis factor-α. Supplementation with either SFV or DAC produced mild improvement in the tissue and laboratory markers. Moreover, the combination of both drugs greatly refined the pathology induced by TAA at the cellular and laboratory levels. However, there are still significant differences when compared to the control. In conclusion, SFV and DAC combination partially but greatly ameliorated the renal damage induced by TAA which might be enhanced with further supplementations to give new hope for those with nephropathy associated with hepatitis.