• Title/Summary/Keyword: Tulasnella calospora

Search Result 3, Processing Time 0.019 seconds

Identification of Orchid Mycorrhizal Fungi Isolated from Epipactis thunbergii in Korea (닭의난초(Epipactis thunbergii)에 공생하는 난 균근균의 분리 및 동정)

  • Han, Han-Kyeol;Chung, Jae-Min;Cho, Yong-Chan;Kim, Dae-Shin;Eom, Ahn-Heum
    • The Korean Journal of Mycology
    • /
    • v.41 no.1
    • /
    • pp.9-13
    • /
    • 2013
  • In this study, roots of Epipactis thunbergii were collected from Chujado on the north of Jeju-do. Six fungal isolates were isolated from surface-sterilized roots of the orchid and classified with groups based on morphological characteristics. Fungal DNA was extracted from each isolate and amplified ITS region using ITS1-OF/ITS4-OF primer pair. Three species of orchid mycorrhizal fungi were identified as Tulasnella calospora, Tulasnella sp. and Sebacina sp. based on molecular and morphological characteristics.

Identification of Orchid Mycorrhizal Fungi Isolated from Terrestrial Orchids in Mt. Hambaek, Korea (함백산의 난초과 식물의 뿌리에서 난균근균의 분리 및 동정)

  • Lee, Bong-Hyung;Han, Han-Kyeol;Eom, Ahn-Heum
    • The Korean Journal of Mycology
    • /
    • v.43 no.2
    • /
    • pp.129-132
    • /
    • 2015
  • In this study, orchid mycorrhizal fungi (OMF) were isolated from four terrestrial orchids on Mt. Hambeak, Platnathera chlorantha, Platnathera mandarinorum, Cephalanthera falcate, and Cephalanthera longibracteata. OMF were identified using morphological and sequences analysis of fungal internal transcribed spacer (ITS) regions by specific primer of basidiomycetous orchid mycorrhizas; ITS1-OF and ITS4-OF. Four species of orchid mycorrhizal fungi were identified as Ceratobasidium sp, Epulorhiza anaticula, Tulasnella calospora and Tulasnella sp.

Identification of Orchid Mycorrhizal Fungi Isolated from Five Species of Terrestrial Orchids in Korea (한국에 자생하는 5종의 지생란에서 분리한 난균근균의 동정)

  • Youm, Jae-Young;Han, Han-Kyeol;Chung, Jae-Min;Cho, Yong-Chan;Lee, Byung-Chun;Eom, Ahn-Heum
    • The Korean Journal of Mycology
    • /
    • v.40 no.3
    • /
    • pp.132-135
    • /
    • 2012
  • This study was performed to isolate and identify the orchid mycorrhizal fungi (OMF) from roots of five species of terrestrial orchids in Korea; Cymbidium goeringii, Spiranthes sinensis, Calanthe discolor, Bletilla striata, Pogonia minor. DNA was extracted from isolated OMF and ITS region was amplified using primers, ITS1-OF and ITS4-OF. Four species of OMF belong to Tulasnellaceae and Sebacinaceae were identified; Tulasnella calospora, Tulasnella irregularis, Tulasnella sp., Sebacina vermifera.