• 한국기계가공학회지
• /
• 제9권2호
• /
• pp.60-65
• /
• 2010

Injection molding process is one of the popular manufacturing methods to produce plastic parts with high efficiency and low cost. The tub-drum for drum type washer is made by an insert injection molding process with aluminum alloy insert of windmill type and has a big and complex structure consisted of many ribs to sustain the strength. In this paper, the volumetric shrinkages of rib part and bottom part surrounded by a windmill type insert are analyzed according to the vertical and circumferential direction of tub-drum. Volumetric shrinkage and its difference according to the height or radius of tub drum inform the designer to reduce the warpage of tub drum, and the optimal design of tub drum can be done from the those results. The change of volumetric shrinkage according to packing pressure is also analyzed. It is very important to analyze the volumetric shrinkage of tub drum because it generates the wearing phenomena at the rotating part connected to an aluminum alloy insert due to the warpage of tub drum.

• BMB Reports
• /
• 제50권1호
• /
• pp.37-42
• /
• 2017

The tubby protein (Tub), a putative transcription factor, plays important roles in the maintenance and function of neuronal cells. A splicing defect-causing mutation in the 3'-end of the tubby gene, which is predicted to disrupt the carboxy-terminal region of the Tub protein, causes maturity-onset obesity, blindness, and deafness in mice. Although this pathological Tub mutation leads to a loss of function, the precise mechanism has not yet been investigated. Here, we found that the mutant Tub proteins were mostly localized to puncta found in the perinuclear region and that the C-terminus was important for its solubility. Immunocytochemical analysis revealed that puncta of mutant Tub co-localized with the aggresome. Moreover, whereas wild-type Tub was translocated to the nucleus by extracellular signaling, the mutant forms failed to undergo such translocation. Taken together, our results suggest that the malfunctions of the Tub mutant are caused by its misfolding and subsequent localization to aggresomes.

• 대한설비공학회지:설비저널
• /
• 제10권1호
• /
• pp.1-11
• /
• 1981

In this paper, a new performance equation of bath tubs has been derived, which is very characteristically illuminating and in good agreement with experiments : $$T=T_{\infty}+(T_0-T_{\infty})e-\frac{k(A'_f+A_0)}{Mc_{P{\Delta}x}t$$, where $T_{\infty}$ is the temperature of the bathroom, $T_0$ that of the bathwater at t=0, k the overall heat conductivity of the tub- wall, $A'_f$ the equivalent surface area to the wall, $A_0$ the submerged area of the tub-wall, M mass of the bath-water, $C_p$ the specific heat of the bathwater and ${\Delta}x$ the thickness of the tub-wall. Here the equivalent-free surface area is written as $$A'_f=mA_f,\;m=const.(1-{\phi})^{0.88}$$ : where m is a numerical factor which is determined by a simple experiment and some calculation, {\phi}$ the relative humidity and $A_f$ the real free-surface area. From this study, it has been clarified that cooling of bath-water is mainly due to mass-transfer through evaporation from the free surface and conductive heat loss through the tub-wall is minor, which rather gaily mock at common sense. The effect of keeping bathwater warn by increase of the tub-wall thickness is also analyzed by a new idea of the thickness gain factor.

• Tuberculosis and Respiratory Diseases
• /
• 제68권4호
• /
• pp.236-239
• /
• 2010

Hot tub lung is a lung disorder associated with exposure to hot tub water contaminated with Mycobacterium avium complex (MAC). Although its pathogenesis remains unclear, it may be considered hypersensitivity pneumonitis (HP) rather than an infectious disease. We report a case which fulfilled the current diagnostic criteria of hot tub lung. A patient had worked as a cleaner in the public bath for approximately one year and presented with dyspnea for over one month. The computed tomographic finding of bilateral ground glass attenuation and pathologic finding of granulomatous inflammation were consistent with HP. MAC was isolated from bronchoalveolar lavage fluid and hot tub water. After corticosteroid treatment without antimycobacterial medication, the patient improved and there has been no recurrence. The patient has since discontinued working in the public bath.

• 생명과학회지
• /
• 제29권1호
• /
• pp.105-111
• /
• 2019

Histone deacetylase (HDAC)-6은 전사조절 및 세포질 내 다양한 단백질들과의 상호작용을 통하여 난소암의 유발에 관여한다. 최근, HDAC-6을 표적으로 하는 특이적 억제제를 활용하여 암세포의 신호전달경로를 차단함으로써 새로운 항암제로서의 개발을 모색하고 있다. 특히, 난소암 치료를 위한 화학요법에서는 생식세포에 미치는 영향이 하나의 중요한 난제가 될 수 있다. 그러나, HDAC-6 억제제가 난소암세포 이외의 생식세포에 미치는 영향에 대한 연구는 아직 미흡한 실정이다. 따라서, 본 연구에서는 HDAC-6 억제제의 하나인 tubastatin A (TubA)가 생쥐의 난소 내 미성숙 난자에 미치는 영향을 RNA sequencing 분석을 통하여 검증하였다. 이러한 유전자 집합을 이용한 통계적 분석은 기존의 개별 유전자분석의 한계를 극복하여 대량의 생물학적 정보를 산출함으로써, 세포 내 신호전달경로와 같은 복잡한 생물학적 변화상태를 보다 더 광범위하고 민감하게 파악할 수 있을 뿐만 아니라 의미있는 결과의 도출에 도움을 줄 수 있다. Gene set enrichment analysis (GSEA) 결과, 세포주기와 감수분열의 조절 및 진행에 관여하는 gene sets의 발현이 germinal vesicle (GV)과 비교하여 TubA 처리군에서 대부분 감소되었다. 또한, ingenuity pathway analysis (IPA)를 통하여 TubA가 난모세포 내 p53 및 pRB의 발현을 증가시키고 CDK4/6 및 cyclin D의 발현을 감소시킬 뿐만 아니라, G2/M 단계의 DNA checkpoint 조절에 관여하는 유전자들의 발현을 증가시킴을 확인하였다. 이러한 결과는 TubA가 난소 내 미성숙 난자의 DNA 손상과 세포주기 관련 신호전달경로 유전자들의 발현변화를 유도함으로써, 세포주기의 중지와 세포사멸을 초래할 수 있음을 제시한다. 따라서, 특히 생식주기 이전의 난소암을 표적으로 하는 HDAC-6 억제제를 이용한 항암제의 개발에 있어 난소 내 미성숙 난자의 정상적인 성장과 발달을 위한 대안적 고려가 필요할 것으로 사료된다.

• Molecules and Cells
• /
• 제44권8호
• /
• pp.591-601
• /
• 2021

Cilia are highly specialized organelles that extend from the cell membrane and function as cellular signaling hubs. Thus, cilia formation and the trafficking of signaling molecules into cilia are essential cellular processes. TULP3 and Tubby (TUB) are members of the tubby-like protein (TULP) family that regulate the ciliary trafficking of G-protein coupled receptors, but the functions of the remaining TULPs (i.e., TULP1 and TULP2) remain unclear. Herein, we explore whether these four structurally similar TULPs share a molecular function in ciliary protein trafficking. We found that TULP3 and TUB, but not TULP1 or TULP2, can rescue the defective cilia formation observed in TULP3-knockout (KO) hTERT RPE-1 cells. TULP3 and TUB also fully rescue the defective ciliary localization of ARL13B, INPP5E, and GPR161 in TULP3 KO RPE-1 cells, while TULP1 and TULP2 only mediate partial rescues. Furthermore, loss of TULP3 results in abnormal IFT140 localization, which can be fully rescued by TUB and partially rescued by TULP1 and TULP2. TUB's capacity for binding IFT-A is essential for its role in cilia formation and ciliary protein trafficking in RPE-1 cells, whereas its capacity for PIP₂ binding is required for proper cilia length and IFT140 localization. Finally, chimeric TULP1 containing the IFT-A binding domain of TULP3 fully rescues ciliary protein trafficking, but not cilia formation. Together, these two TULP domains play distinct roles in ciliary protein trafficking but are insufficient for cilia formation in RPE-1 cells. In addition, TULP1 and TULP2 play other unknown molecular roles that should be addressed in the future.

• Mycobiology
• /
• 제31권1호
• /
• pp.42-45
• /
• 2003

For transformation of Pleurotus ostreatus, two novel vectors, pPhKM1 and pPhKM2, were constructed, using the regulatory sequences of the P. sajor-caju $\beta$-tubulin gene(TUB1) and the ble gene encoding phleomycin binding protein. pPhKM1 contains ble fused to the TUB1 promoter and the Schizophyllum commune GPD terminator. pPhKM2 contains ble fused to the promoter and terminator regions of P. sajor-caju TUB1. To confirm phleomycin-resistance activity, each vector was cotrans-formed with pTRura3-2 into the P. ostreatus homokaryotic $ura^-$ strain. The transforming DNA was stably integrated into the genomic DNA. Subsequently, phleomycin resistance was conferred on wild-type dikaryotic P. ostreatus by transformation with pPhKM1 or pPhKM2. This transformation system generated stable phleomycin-resistant transformants.

• 한국건축시공학회:학술대회논문집
• /
• 한국건축시공학회 2012년도 춘계 학술논문 발표대회
• /
• pp.169-171
• /
• 2012

Although work-efficiency of construction machinery is a critical factor for estimating its workable-quantity per unit time, the coefficient figure table presented in the Poom-Sam that is used for Construction Cost Estimation of public sectors in Korea is very subjective for practical usage. In order to suggest objective work-coefficient table for a Tub Grinder, domestic and overseas documentary records were investigated and on-going construction sites were also visited. The research found that the table can be revised by means of detailing down by several factors. The research will be the foundation for applying the rapid development of Construction Equipment and technology to the appropriate cost estimations and the ground work of related studies.

• E2M - 전기 전자와 첨단 소재
• /
• 제5권3호
• /
• pp.320-327
• /
• 1992

Twin-tub CMOS 공정에 의해 제작된 서브마이크로미터 채널길이를 갖는 n채널 및 p채널 MOSFET의 특성을 고찰하였다. n채널 및 p채널 영역에서의 불순물 프로파일과 채널 이온주입 조건에 따른 문턱전압의 의존성 및 퍼텐셜 분포를 SUPREM-II와 MINIMOS 4.0을 사용하여 시뮬레이션하였다. 문턱전압 조정을 위한 counter-doped 보론 이온주입에 의해 p채널 MOSFET는 표면에서 대략 0.15.mu.m의 깊이에서 매몰채널이 형성되었다. 각 소자의 측정 결과, 3.3[V] 구동을 위한 충분한 여유를 갖는 양호한 드레인 포화 특성과 0.2[V]이하의 문턱전압 shift를 갖는 최소화된 짧은 채널 효과, 10[V]이상의 높은 펀치쓰루 전압과 브레이크다운 전압, 낮은 subthreshold 값을 얻었다.

• 한국미생물·생명공학회지
• /
• 제49권3호
• /
• pp.458-465
• /
• 2021

Tubulysin은 다양한 암세포주에 대해 강한 항암활성을 보이는 점액세균 유래 이차대사 생리활성물질이다. 본 연구에서는 tubulysin을 생산하는 두 균주의 점액세균 Archangium gephyra MEHO_002와 MEHO_004의 유전체 분석을 통해 tubulysin 생합성 유전자들로 추정되는 유전자군을 발견하였으며, 플라스미드 삽입에 의한 유전자 불활성화를 통해 이들 유전자들이 tubulysin 생산과 직접 연관되어 있음을 확인하였다. A. gephyra MEHO_002와 MEHO_004 균주의 tubulysin 생합성 유전자군(tubA~tubF)은 DNA 염기서열이 서로 97% 동일하였으며, 암호화하는 단백질들의 아미노산 서열도 서로 97-100% 유사하였다. MEHO_002와 MEHO_004 균주의 tubulysin 생합성 유전자군은 tubulysin 생산 점액세균으로 알려진 Cystobacter sp. SBCb004의 tubulysin 생합성 유전자군과 DNA 염기서열이 86% 동일하였다. 유전자군의 구성은 tubZ 유전자가 존재하지 않는다는 점을 제외하고는 SBCb004의 tubulysin 생합성 유전자군 구성과 동일하였다. 각 유전자가 암호화하는 단백질의 아미노산 서열은 Cystobacter sp. SBCb004의 tubulysin 생합성 유전자가 암호화하는 단백질들과 88-97% 유사하였으며, 각 단백질들의 도메인 구성도 동일하였다.