• Journal of the Korean Society of Manufacturing Process Engineers
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• v.9 no.2
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• pp.60-65
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• 2010

Injection molding process is one of the popular manufacturing methods to produce plastic parts with high efficiency and low cost. The tub-drum for drum type washer is made by an insert injection molding process with aluminum alloy insert of windmill type and has a big and complex structure consisted of many ribs to sustain the strength. In this paper, the volumetric shrinkages of rib part and bottom part surrounded by a windmill type insert are analyzed according to the vertical and circumferential direction of tub-drum. Volumetric shrinkage and its difference according to the height or radius of tub drum inform the designer to reduce the warpage of tub drum, and the optimal design of tub drum can be done from the those results. The change of volumetric shrinkage according to packing pressure is also analyzed. It is very important to analyze the volumetric shrinkage of tub drum because it generates the wearing phenomena at the rotating part connected to an aluminum alloy insert due to the warpage of tub drum.

• BMB Reports
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• v.50 no.1
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• pp.37-42
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• 2017

The tubby protein (Tub), a putative transcription factor, plays important roles in the maintenance and function of neuronal cells. A splicing defect-causing mutation in the 3'-end of the tubby gene, which is predicted to disrupt the carboxy-terminal region of the Tub protein, causes maturity-onset obesity, blindness, and deafness in mice. Although this pathological Tub mutation leads to a loss of function, the precise mechanism has not yet been investigated. Here, we found that the mutant Tub proteins were mostly localized to puncta found in the perinuclear region and that the C-terminus was important for its solubility. Immunocytochemical analysis revealed that puncta of mutant Tub co-localized with the aggresome. Moreover, whereas wild-type Tub was translocated to the nucleus by extracellular signaling, the mutant forms failed to undergo such translocation. Taken together, our results suggest that the malfunctions of the Tub mutant are caused by its misfolding and subsequent localization to aggresomes.

• The Magazine of the Society of Air-Conditioning and Refrigerating Engineers of Korea
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• v.10 no.1
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• pp.1-11
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• 1981

In this paper, a new performance equation of bath tubs has been derived, which is very characteristically illuminating and in good agreement with experiments : $$T=T_{\infty}+(T_0-T_{\infty})e-\frac{k(A'_f+A_0)}{Mc_{P{\Delta}x}t$$, where $T_{\infty}$ is the temperature of the bathroom, $T_0$ that of the bathwater at t=0, k the overall heat conductivity of the tub- wall, $A'_f$ the equivalent surface area to the wall, $A_0$ the submerged area of the tub-wall, M mass of the bath-water, $C_p$ the specific heat of the bathwater and ${\Delta}x$ the thickness of the tub-wall. Here the equivalent-free surface area is written as $$A'_f=mA_f,\;m=const.(1-{\phi})^{0.88}$$ : where m is a numerical factor which is determined by a simple experiment and some calculation, {\phi}$ the relative humidity and $A_f$ the real free-surface area. From this study, it has been clarified that cooling of bath-water is mainly due to mass-transfer through evaporation from the free surface and conductive heat loss through the tub-wall is minor, which rather gaily mock at common sense. The effect of keeping bathwater warn by increase of the tub-wall thickness is also analyzed by a new idea of the thickness gain factor.

• Tuberculosis and Respiratory Diseases
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• v.68 no.4
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• pp.236-239
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• 2010

Hot tub lung is a lung disorder associated with exposure to hot tub water contaminated with Mycobacterium avium complex (MAC). Although its pathogenesis remains unclear, it may be considered hypersensitivity pneumonitis (HP) rather than an infectious disease. We report a case which fulfilled the current diagnostic criteria of hot tub lung. A patient had worked as a cleaner in the public bath for approximately one year and presented with dyspnea for over one month. The computed tomographic finding of bilateral ground glass attenuation and pathologic finding of granulomatous inflammation were consistent with HP. MAC was isolated from bronchoalveolar lavage fluid and hot tub water. After corticosteroid treatment without antimycobacterial medication, the patient improved and there has been no recurrence. The patient has since discontinued working in the public bath.

• Journal of Life Science
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• v.29 no.1
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• pp.105-111
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• 2019

In recent years, progress has been made in the search for the development of new anti-cancer agents by employing specific inhibitors of histone deacetylase (HDAC)-6 to block signal transduction pathways in cancer cells. This study examined the effects of tubastatin A (TubA), an HDAC-6 inhibitor, on the growth and development of immature oocytes in murine ovaries using RNA sequencing analysis. The results from a gene set enrichment analysis (GSEA) indicated that the expression of most of the gene sets involved in the cell cycle and control and progression of meiosis decreased in the TubA-treated group as compared with that in germinal vesicle (GV) stage oocytes. In addition, an ingenuity pathway analysis (IPA) suggested that TubA not only caused increased expression of p53 and pRB and decreased expression of CDK4/6 and cyclin D but also caused elevated expression of genes involved in the control of the DNA check point in G2/M stage oocytes. These results suggest that TubA may induce cell cycle arrest and apoptosis through the induction of changes in the expression of genes involved in signal transduction pathways associated with DNA damage and the cell cycle of immature oocytes in the ovary.

• Molecules and Cells
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• v.44 no.8
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• pp.591-601
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• 2021

Cilia are highly specialized organelles that extend from the cell membrane and function as cellular signaling hubs. Thus, cilia formation and the trafficking of signaling molecules into cilia are essential cellular processes. TULP3 and Tubby (TUB) are members of the tubby-like protein (TULP) family that regulate the ciliary trafficking of G-protein coupled receptors, but the functions of the remaining TULPs (i.e., TULP1 and TULP2) remain unclear. Herein, we explore whether these four structurally similar TULPs share a molecular function in ciliary protein trafficking. We found that TULP3 and TUB, but not TULP1 or TULP2, can rescue the defective cilia formation observed in TULP3-knockout (KO) hTERT RPE-1 cells. TULP3 and TUB also fully rescue the defective ciliary localization of ARL13B, INPP5E, and GPR161 in TULP3 KO RPE-1 cells, while TULP1 and TULP2 only mediate partial rescues. Furthermore, loss of TULP3 results in abnormal IFT140 localization, which can be fully rescued by TUB and partially rescued by TULP1 and TULP2. TUB's capacity for binding IFT-A is essential for its role in cilia formation and ciliary protein trafficking in RPE-1 cells, whereas its capacity for PIP₂ binding is required for proper cilia length and IFT140 localization. Finally, chimeric TULP1 containing the IFT-A binding domain of TULP3 fully rescues ciliary protein trafficking, but not cilia formation. Together, these two TULP domains play distinct roles in ciliary protein trafficking but are insufficient for cilia formation in RPE-1 cells. In addition, TULP1 and TULP2 play other unknown molecular roles that should be addressed in the future.

• Mycobiology
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• v.31 no.1
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• pp.42-45
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• 2003

For transformation of Pleurotus ostreatus, two novel vectors, pPhKM1 and pPhKM2, were constructed, using the regulatory sequences of the P. sajor-caju $\beta$-tubulin gene(TUB1) and the ble gene encoding phleomycin binding protein. pPhKM1 contains ble fused to the TUB1 promoter and the Schizophyllum commune GPD terminator. pPhKM2 contains ble fused to the promoter and terminator regions of P. sajor-caju TUB1. To confirm phleomycin-resistance activity, each vector was cotrans-formed with pTRura3-2 into the P. ostreatus homokaryotic $ura^-$ strain. The transforming DNA was stably integrated into the genomic DNA. Subsequently, phleomycin resistance was conferred on wild-type dikaryotic P. ostreatus by transformation with pPhKM1 or pPhKM2. This transformation system generated stable phleomycin-resistant transformants.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2012.05a
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• pp.169-171
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• 2012

Although work-efficiency of construction machinery is a critical factor for estimating its workable-quantity per unit time, the coefficient figure table presented in the Poom-Sam that is used for Construction Cost Estimation of public sectors in Korea is very subjective for practical usage. In order to suggest objective work-coefficient table for a Tub Grinder, domestic and overseas documentary records were investigated and on-going construction sites were also visited. The research found that the table can be revised by means of detailing down by several factors. The research will be the foundation for applying the rapid development of Construction Equipment and technology to the appropriate cost estimations and the ground work of related studies.

• Electrical & Electronic Materials
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• v.5 no.3
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• pp.320-327
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• 1992

Twin-tub CMOS 공정에 의해 제작된 서브마이크로미터 채널길이를 갖는 n채널 및 p채널 MOSFET의 특성을 고찰하였다. n채널 및 p채널 영역에서의 불순물 프로파일과 채널 이온주입 조건에 따른 문턱전압의 의존성 및 퍼텐셜 분포를 SUPREM-II와 MINIMOS 4.0을 사용하여 시뮬레이션하였다. 문턱전압 조정을 위한 counter-doped 보론 이온주입에 의해 p채널 MOSFET는 표면에서 대략 0.15.mu.m의 깊이에서 매몰채널이 형성되었다. 각 소자의 측정 결과, 3.3[V] 구동을 위한 충분한 여유를 갖는 양호한 드레인 포화 특성과 0.2[V]이하의 문턱전압 shift를 갖는 최소화된 짧은 채널 효과, 10[V]이상의 높은 펀치쓰루 전압과 브레이크다운 전압, 낮은 subthreshold 값을 얻었다.

• Microbiology and Biotechnology Letters
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• v.49 no.3
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• pp.458-465
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• 2021

Tubulysins are a group of bioactive secondary metabolites from myxobacteria exhibiting strong anticancer activity against various cancer cell lines. In this study, we describe the identification of putative tubulysin biosynthetic gene clusters (tubA~tubF) in the genome sequences of two tubulysin-producing myxobacterial strains, Archangium gephyra MEHO_002 and MEHO_004. The inactivation of the putative tubulysin biosynthetic genes resulted in a tubulysin-production defect. The DNA sequences of the A. gephyra MEHO_002 and MEHO_004 tubulysin biosynthetic genes were 97% identical, and the amino acid sequences of the encoded proteins shared a similarity of 97-100%. The nucleotide sequences of the tubulysin biosynthetic gene clusters in MEHO_002 and MEHO_004 were 86% identical to that in Cystobacter sp. SBCb004 known as a tubulysin-producing myxobacterium, and the organization of the clusters was identical except for the lack of a tubZ gene in the clusters in MEHO_002 and MEHO_004. The amino acid sequences of the proteins encoded by each gene were 88-97% similar to those encoded by SBCb004, and the domain compositions of the proteins were also identical.