• The Korean Journal of Physiology and Pharmacology
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• v.22 no.3
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• pp.343-348
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• 2018

Recent human genetic studies have shown that $G{\beta}5$ is related to various clinical symptoms, such as sinus bradycardia, cognitive disability, and attention deficit hyperactivity disorder. Although the calcium signaling cascade is closely associated with a heterotrimeric G-protein, the function of $G{\beta}5$ in calcium signaling and its relevance to clinical symptoms remain unknown. In this study, we investigated the in vitro changes of store-operated calcium entry (SOCE) with exogenous expression of $G{\beta}5$. The cells expressing $G{\beta}5$ had enhanced SOCE after depletion of calcium ion inside the endoplasmic reticulum. $G{\beta}5$ also augmented Stim1- and Orai1-dependent SOCE. An ORAI1 loss-of-function mutant did not show inhibition of $G{\beta}5$-induced SOCE, and a STIM1-ERM truncation mutant showed no enhancement of SOCE. These results suggested a novel role of GNB5 and Stim1, and provided insight into the regulatory mechanism of SOCE.

• IMMUNE NETWORK
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• v.2 no.2
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• pp.72-78
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• 2002

Background: The precise mechanism by which CTLA-4 regulates T cell immune responses is still not fully understood. Previously we proposed that CTLA-4 could downregulate T cell function by modulating a signaling cascade initiated from the T cell receptor complex. The evidence for this notion comes from our findings that CTLA-4 associated with the T cell receptor zeta (TCR zeta) chain, and hence regulated TCR zeta phosphorylation by co-associated SHP-2 tyrosine phosphatase (1). In this report, we investigated whether any other signaling molecules could be involved in the CTLA-4 signaling pathway. Methods: We have taken biochemical approaches, such as immunoprecipitation followed by autoradiography or immunoblotting, to identify the molecules associated with CTLA-4. To perform these assays, we used activated primary T cells and ectopically transfected 293 cells. Various truncation mutants of CTLA-4 were used to map the interaction site on CTLA-4. Results: We found that in addition to TCR zeta and SHP-2, a recently cloned small adaptor molecule, SAP (SLAM-associated protein), was also able to associate with CTLA-4. We identified the domain of SAP association in CTLA-4 being a motif involving GVYVKM. This motif has been previously found to bind SHP-2 through its phosphorylated tyrosine interaction with SH-2 domain of SHP-2. Indeed, co-expression of SAP and SHP-2 reduced their binding to CTLA-4 significantly, suggesting that SAP and SHP-2 compete for the common binding site, GVYVKM. Thus, by blocking SHP-2 recruitment SAP could function as a negative regulator of CTLA-4. Conclusion: Taken together, our data suggest the existence of complicate signaling cascade in regulating CTLA-4 function, and further provide evidence that SAP can act either as a positive or negative regulator depending on the nature of the associating receptors.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2009.10a
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• pp.258-263
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• 2009

This paper describes an analysis of the stability and performance of a large-capacity flywheel energy storage system (FESS) supported by active magnetic bearings. We designed and manufactured the system that can store up to 5kWh of usable energy at the maximum speed of 18,000 rpm. In order to analyze the stability of the systems accurately, we derived a rigid body rotor model, flexible rotor model using finite-element method, and a reduced-order model using modal truncation. The rotor model is combined with those of active magnetic bearings, amplifiers, and position sensors, resulting in a system simulation model. This simulation model is validated against experimental measurements. The stability of the system is checked from the pole locations of the closed-loop transfer functions. We also investigated the sensitivity function to quantify the robustness of the systems to the disturbances such as mass imbalance and sensor noises.

• Transactions of the Korean Society of Mechanical Engineers
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• v.12 no.2
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• pp.338-348
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• 1988

This study presents an analysis of periodic heat diffusion in an annular fin with uniform thickness. When the temperature of the fin base is changed in the form of a sinusoidal function, the exact temperature solution can be obtained by Laplace transformation in terms of the dimensionless parameters in the infinite series. Local heat flux and average heat flux, local fin efficiency and average fin efficiency were obtained. Particularly, the table of eigenvalues that are the indispensable condition in solving the heat transfer problem of annular fin in a transient state with convection phenomena at the fin edge is provided. The tables of heat fluxes and average heat fluxes, fin efficiencies and average fin efficiencies are also provided from the computed results. Also, substituting the variations of dimensionless parameters into the these exact solutions, the characteristics of these response are investigated.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.11 no.3
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• pp.149-155
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• 1999

A treatment of wave breaking is included in the extended Boussinesq equations of Nwogu. A spatially distributed source function and sponge layers are used to reduce the reflected waves in the computa¬tional domain. The model uses fourth-order Adams predictor-corrector method to advance in time, and discretizes first-order spatial derivatives to fourth-order accuracy, and thus reducing all truncation errors to a level smaller than the dispersive terms. The generated wave fields are found to be good and the corresponding wave heights are very close to their target values. For the tests of wave breaking, although agreement is considered to be reasonable, wave heights in the inner surf zone are over-predicted. This indicates the breaking parameters in the model should be adjusted.

• Journal of applied mathematics & informatics
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• v.15 no.1_2
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• pp.29-51
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• 2004

In this paper, we propose a new mixed finite element method, called the characteristics-mixed method, for approximating the solution to Burgers' equation. This method is based upon a space-time variational form of Burgers' equation. The hyperbolic part of the equation is approximated along the characteristics in time and the diffusion part is approximated by a mixed finite element method of lowest order. The scheme is locally conservative since fluid is transported along the approximate characteristics on the discrete level and the test function can be piecewise constant. Our analysis show the new method approximate the scalar unknown and the vector flux optimally and simultaneously. We also show this scheme has much smaller time-truncation errors than those of standard methods. Numerical example is presented to show that the new scheme is easily implemented, shocks and boundary layers are handled with almost no oscillations. One of the contributions of the paper is to show how the optimal error estimates in $L^2(\Omega)$ are obtained which are much more difficult than in the standard finite element methods. These results seem to be new in the literature of finite element methods.

• Journal of Korean Library and Information Science Society
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• v.35 no.1
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• pp.303-317
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• 2004

The purpose of this study was to develop a framework for predicting searching performance through an understanding of how cognitive ability relates to searching process and outcome. Specifically, this study examined the relationship between spatial visualization, logical reasoning, integrative reasoning, and information searching process and outcome. Information searching process was assessed by seven search process indicators: (1) search command selection: (2) combination of search commands; (3) application of Boolean logic: (4) application of truncation; (5) use of limit search function; (6) number of search statements; and (7) number of search errors made. Searching outcome was assessed by the number of correct answers to search questions. Subjects first took three standardized cognitive tests that measured cognitive abilities, and performed online catalog searching in response to seven information search questions. The searches were logged using Lotus ScreenCam, and reviewed for the analysis. Factor analysis was used to find underlying structures of the seven search process variables. Multiple regression analysis was applied to examine the predictive power of three cognitive variables on three extracted factors, and search outcome. Results of the data analysis showed that individual differences in logical reasoning could predict information searching process and outcome.

• Journal of the Optical Society of Korea
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• v.20 no.3
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• pp.341-357
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• 2016

A novel asymmetric multiple-image encryption method using an octonion Fresnel transform (OFST) and a two-dimensional Sine Logistic modulation map (2D-SLMM) is presented. First, a new multiple-image information processing tool termed the octonion Fresneltransform is proposed, and then an efficient method to calculate the OFST of an octonion matrix is developed. Subsequently this tool is applied to process multiple plaintext images, which are represented by octonion algebra, holistically in a vector manner. The complex amplitude, formed from the components of the OFST-transformed original images and modulated by a random phase mask (RPM), is used to derive the ciphertext image by employing an amplitude- and phase-truncation approach in the Fresnel domain. To avoid sending whole RPMs to the receiver side for decryption, a random phase mask generation method based on SLMM, in which only the initial parameters of the chaotic function are needed to generate the RPMs, is designed. To enhance security, the ciphertext and two decryption keys produced in the encryption procedure are permuted by the proposed SLMM-based scrambling method. Numerical simulations have been carried out to demonstrate the proposed scheme's validity, high security, and high resistance to various attacks.

• 제어로봇시스템학회:학술대회논문집
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• 1998.10a
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• pp.285-288
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• 1998

A least-squares identification method is studied that estimates a finite number of coefficients in the series expansion of a transfer function, where the expansion is in terms of recently introduced generalized basis functions, We will expand and generalize the orthogonal functions as basis functions for dynamical system representations. To this end, use is made of balanced realizations as inner transfer functions. The orthogonal functions can be considered as generalizations of, for example, the pulse functions, Laguerre functions, and Kautz functions, and give rise to an alternative series expansion of rational transfer functions. We show that the Laplace transform of the expansion for some sets$\Psi_{\kappa}(Z)$ is equivalent to a series expansion . Techniques based on this result are presented for obtaining the coefficients $c_{n}$ as those of a series. One of their important properties is that, if chosen properly, they can substantially increase the speed of convergence of the series expansion. This leads to accurate approximate models with only a few coefficients to be estimated. The set of Kautz functions is discussed in detail and, using the power-series equivalence, the truncation error is obtained.

• Journal of Digital Convergence
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• v.13 no.1
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• pp.283-288
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• 2015

A disposable password is necessary to avoid any danger by the use of a static password and reinforce the user's authentication. In order to prevent personal information from being exposed, OTP generation algorithm is regarded as important. The OTP generation algorithm we suggest in this thesis generates 256-bit-size OTP Data by using Seed value and Time value. This value that the generated OTP Data are arranged with a matrix and a 32-bit-value is extracted on an irregular basis becomes the final value. We can find out that the more OTP generation frequency we have, the lower probability of clash tolerance we get in our suggested algorithm, compared to the previous algorithm.