• Journal of Microbiology
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• 제40권4호
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• pp.289-294
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• 2002

The fungal strain SW-3 having antimicrobial activity was isolated from soil of crucified plants in Pocheon, Kyungki-Do, Korea. Strain SW-3 was identified as Alternaria brassicicola by its morphological characteristics, and confirmed by the analysis of the 18S gene and ITS regions of rDNA. The fungus showed a similarity of 99% with Alternaria brassicicola in the 18S rDNA sequence analysis. A. brassicicola has been reported to produce an antitumor compound, called depudecin. We found that strain SW-3 produced antimicrobial metabolites, in addition to depudecin, during sporulation under different growth conditions. The metabolite of the isolated fungus was found to have strong antifungal activity against Microsporium canis and Trichophyton rubrum, and antibacterial activity against Staphylococcus aureus and Pseudomonas aerogenes. The amount and kind of metabolites produced by the isolate were affected by growth conditions such as nutrients and growth periods.

• 대한임상검사과학회지
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• 제42권2호
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• pp.81-85
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• 2010

The clinical features of 178 patients with tinea corporis from January to December 2008 in Catholic Skin Clinics were evaluated. KOH examination and culture from the patients lesions were performed. And cultured dermatophytes were identified by colony morphology and microscopic findings. The ratio of male to female with the infection was about 1.3:1 and showed more prevalence in male. The unexposed area (84.5%) was more frequently affected than exposed (15.5%) and the most common site was buttock. Coexisting dermatophytoses in patients with tinea corporis were noted in 27 cases and the most commons were tinea pedis. The isolated dermatophytes were composed of Trichophyton rubrum (91.7%), Microsporum canis (2.6%), T. verrucosum (2.6%), T. mentagrophytes (1.7%), and T. tonsurans (1.7%).

• 생약학회지
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• 제25권4호통권99호
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• pp.319-327
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• 1994

A new diterpene, agastanol[1] with dehydroagastol[2] was isolated from th root of Agastache rugosa, and their structures were elucidated by chemical and instrumental analysis. Agastanol[1], its derivatives, agastanone[3] and methylagastanol[5], and dehydroagastol[2] showed cytotoxic activites against in vitro human cancer cell lines. Agastanol[1] showed weak antifungal activity against Trichophyton rubrum.

• 미생물학회지
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• 제29권6호
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• pp.353-360
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• 1991

5-substituted uridine(I,Br,Cl), 5'-amino-5'-deoxyuridine conjugates of amino acid, peptide and penicillin G, 5'-monophosphate uridine derivatives and 5'-monophosphate-fatty acid detrivatives were chemically synthesized. Their biological activities were determined as MIC and IC/sub 50/ unit against various pathogenic microorganisms in vitro. 5'-amino-5'-deoxyuridine-cyclo(Phe-Asp)(23), 5-iodo-5'-amino-deoxyuridine-penicillin G(26) were the most efficient; their IC/sub 50/ against L5178Y murine lymphoma cell was 6.5 h/ml, MIC against S. aureus (+) and E. coli (-) was 6.25 g/ml. MIC of 5-bromo-2', 3'-O-isopropylideneuridine(6) against Trichophyton rubrum was 0.2 g/ml. And 5'-monophosphate derivatives are more active than simple uridine derivatives, suggesting other modified nucleoside 5'-phosphate may be worthwhile examing further as a new prodrugs.

• Mycobiology
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• 제37권2호
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• pp.82-88
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• 2009

The mycoflora on the hair in 25 samples of each of goats and sheep collected from Libya was analyzed using two isolation methods at $25^{\circ}C$. Seventy species and 3 varieties belonging to 31 genera were collected from the two substrates. The hairs of sheep were polluted with fungi than goat, contained high total counts and number of genera and species. Two species of true dermatophytes were isolated namely Trichophyton rubrum and T. terrestre. Several keratiophilic species were isolated of which Chrysosporium indicum, C. keratinophilum and C. tropicum were the most prevalent. The commonest saprophytes in order of frequency were members of the genera Aspergillus, Penicillium, Emericella, Alternaria and Cochliobolus.

• 한국미생물·생명공학회지
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• 제1권1호
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• pp.43-50
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• 1973

34종의 Hexachlorophene유도체가 합성되었으며 그 각각의 화합물에 대하여 항균성시험이 행해졌다. 검정균은 세종류의 세균과 무좀균(백성균)을 포함한 5종류의 진균류이다. 시험결과는 1. 2,2'-Methylene bis [$\alpha$-(3,4,6-trichlorophenoxy)-$\beta$-(N,N-diethylamino) propionic acid] 및 2,2'-methylene bis [$\alpha$-3,4,6-trichlorophenoxy)-$\beta$-(N,N-dimethylamino) propionic acid] 는 Sta. aureus와 B.subtilis에 대해 강한 항균작용을 나타냈다. 2. 2,2'-methylene bis [$\alpha$-(3,4,6-trichlorophenoxy)-$\beta$-(m-hydroxy-p-carbozyphenylarnino ) propionic acid]는 특히 진균류의 생장저해작용이 강하며 Trichophyton rubrum 및 Microsporum gypseum에는 2 $\mu\textrm{g}$/$m\ell$의 농도에서 항균성을 가지며, Epidermophyton floccosum Aspergillus niger 및 Aspergillus oryzae에 대해서는 1 $\mu\textrm{g}$/$m\ell$의 농도에서 항균성을 나타냄으로서 Hexachlorophene 항균성의 10배 내지 50배의 항균력을 가진다. 3. 34종류의 합성물질중 원 Hexachlorophene에 비해 5종류의 진균류에 대해 부분적으로나마 강한 항균력을 나타내는 물질은 23종류였고 3종류의 세균에 대해선 13종류였다.

• 한국약용작물학회지
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• 제28권1호
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• pp.38-46
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• 2020

Background: Although Sancho (Zanthoxylum schinifolium Siebold & Zucc) oil has traditionally been used for its antibiotics properties, there is currently a lack of scientific evidence regarding its biological activities. In this study, we investigated the antimicrobial and antioxidant activities of Sancho oil against food-hazardous microorganisms, phytopathogens, and dermatophytes. Methods and Results: We investiated the antimicrobial activity of Sancho oil against 11 food-hazardous microorganisms, nine phytopathogens, and six dermatophytes. The Sancho oil was found to show the strongest antibacterial activity against Shigella flexneri and Listeria spp. Sancho oil also showed high antifungal activity against plant pathogens, particularly Fusarium oxysporum, and showed antimicrobial activity against dermatophytes such as Trichophyton rubrum, Microsporum canis and Candida albicans. The antioxidant activity of Sancho oil was measured using the DPPH method, and was found to be stronger than that of unrefined oil. Moreover, this activity increased with increasing oil concentration. Conclusions: We found that Sancho oil showed differing antimicrobial activities against food-hazardous microorganisms, dermatophytes, and plant pathogens. The antimicrobial activity spectrum of Sancho oil was not broad and varied among microbial strains. On the basis of our findings, we consider that Sancho oil could be used an antibacterial material for food-borne S. flexneri and Listeria spp., a biopesticide for Fusarium spp., and a treatment for dermatophytes such as T. rubrum.

• Journal of the Korean Wood Science and Technology
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• 제38권3호
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• pp.242-250
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• 2010

본 연구는 삼나무의 건조 중 발생하는 Volatile Organic Compounds (VOCs) 중 terpenoids 성분을 분석하기 위해 thermal extractor를 이용하였다. 기존의 건조 조건을 고려하여 $27^{\circ}C$,$60^{\circ}C$, $80^{\circ}C$, $100^{\circ}C$, $120^{\circ}C$에서 실험을 실시한 결과 온도 조건이 높아질수록 VOCs와 terpenoids의 방산량이 증가하였다. 방산된 VOCs 중 terpenoids 화합물이 차지하는 비율은 각각의 온도에 대해 87.5%, 81.6%, 83.6%, 90.1%, 97.3%로 특히$100^{\circ}C$와 $120^{\circ}C$에서는 90% 이상의 높은 비율을 차지하였다. 상대적으로 가장 높은 비율을 차지하고 있는 성분은 ${\delta}$-cadinene이었으며 이 외에도 총 32종류의 다양한 terpenoids 성분이 검출되었다. 특히 방산된 성분들은 모두 탄소 15개로 이루어진 sesquiterpene 류에 속하는 성분으로서 삼나무를 이루는 주요 성분임을 확인하였다. 방산 된 sesquiterpenes 화합물의 이용을 모색하고자 피부사상균에 대한 항진균 활성을 한천희석법을 이용하여 평가 해 보았다. 그 결과 공시 균주 Trichophyton rubrum, Trichophyton mentagrophytes, Microsporum gypseum 에 대해 모두 활성을 나타냈다. 시료의 농도가 5000 ppm일 때에는 3 균주에 대해 모두 100%의 활성을 나타냈으며 1000 ppm일 때에는 95.2%, 98.7%, 97.3%의 활성을, 100 ppm일 때에는 90.5%, 43.6%, 85.9%의 활성을 각각 보였다.

• 대한임상검사과학회지
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• 제37권2호
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• pp.118-122
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• 2005

An epidemiological study was performed on zoophilic dermatophytes. The number of patients with zoophilic dermatophytes diagnosed on clinical findings and culture at the Catholic Dermatological Clinic in Daegu City were 1,339 for 10 years from January 1995 to December 2004. Species of zoophilic dermatophytes isolated from 47,925 patients with dermatophytes were as follows; Trichophyton(T.) rubrum was isolated from 43,702 (91.2%), T. interdigitale 2,660 (5.6%), Epidermophyton(E.) floccosum 43 (0.1%), T. tonsurans 131 (0.3%), T. mentagrophytes 625 (1.3%), T. verrucosum 56 (0.2%), Microsporum(M.) canis 658 (1.4%), and M. gypseum 50 (0.1%). Zoophilic dermatophytes infections were chiefly Tinea facial in the cases of T. mentagrphytes and Tinea corporis in the cases of T. verrucosum, but Tinea capitis in the cases of M. canis. Number of patients with zoophilic dermatophytes by site and age were as follows; Most patients with infected scalps were young people under 15 years old. Infection of the trunk area (neck,chest,back) and extremities (arms, legs, hands, feet) were relatively high in those patients over 15 years old. M. canis were most frequently isolated in autumn and winter, and T. mentagrophyts and T. verrucosum were mostly in spring and winter.

• 대한의생명과학회지
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• 제16권3호
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• pp.187-192
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• 2010

The aim of this study was to compare the results of morphological phenotypic examination and molecular biological method, randomly amplified polymorphic DNA (RAPD) using clinical isolates from Korean patients infected with dermatophytes. A total of 44 clinical isolates have been collected by Korean Collection Medical Fungi (KCMF) and those were subjected for the RAPD analysis. The results showed that the fungal strains that have RG1 band patterns were identified as Trichophyton rubrum by the neighbour-joining analysis. Additionally, other fungal strains that have RG3 band patterns were identified as Epidermophyton floccosum and RG5 band patterns were identified as Micorsporum gypseum. Even though molecular biological method such as RAPD are not necessary, it might be useful when some strains have similar morphological characteristics or when same species has phenotypic variations. In this study, therefore, we targeted internal transcribed spacer (ITS) regions of fungal rDNA, performed RAPD and then compared with morphological phenotypic examination.