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Phylogenic Analysis of Alternaria brassicicola Producing Bioactive Metabolites  

Jung, Dong-Sun (Department of Food and Microbial Technology)
Na, Yeo-Jung (Department of Food and Microbial Technology)
Ryu, Ki-Hyun (Department of Horticultural Science, Seoul Women's University)
Publication Information
Journal of Microbiology / v.40, no.4, 2002 , pp. 289-294 More about this Journal
Abstract
The fungal strain SW-3 having antimicrobial activity was isolated from soil of crucified plants in Pocheon, Kyungki-Do, Korea. Strain SW-3 was identified as Alternaria brassicicola by its morphological characteristics, and confirmed by the analysis of the 18S gene and ITS regions of rDNA. The fungus showed a similarity of 99% with Alternaria brassicicola in the 18S rDNA sequence analysis. A. brassicicola has been reported to produce an antitumor compound, called depudecin. We found that strain SW-3 produced antimicrobial metabolites, in addition to depudecin, during sporulation under different growth conditions. The metabolite of the isolated fungus was found to have strong antifungal activity against Microsporium canis and Trichophyton rubrum, and antibacterial activity against Staphylococcus aureus and Pseudomonas aerogenes. The amount and kind of metabolites produced by the isolate were affected by growth conditions such as nutrients and growth periods.
Keywords
rDNA; A. brassicicola; fungal metabolites; antimicrobial activity;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 1  (Related Records In Web of Science)
Times Cited By SCOPUS : 2
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1 Depudecin: a nol compound inducing the flat phenotype of NIH3T3 cells doubly transformed by ras-and src-onco-gene, produced by Alternaria brassicicola. /
[ Matsumoto,M.;S.Matsutani;K.Sugita;H.Yoshida;F.Hayashi;Y.Terui;H.Nakai;N.Uotani;Y.Kawamura;K.Matsumoto. ] / J. Antibiotics
2 DNASTAR’s Lasergene sequence analysis software. /
[ Burland,T.G. ] / Methods Mol.Biol.
3 RNA/DNA miniprep from a single sample of orchid tissue. /
[ Knapp,J.E.;J.M.Chandlee. ] / BioTechniques
4 Identification and toxicity of Alternaria brassicicola, the causal agent of dark leaf spot disease of Brassica species grown in Thailand. /
[ Pattanamahakul,P.;R.N.Strange. ] / Plant Pathol.   ScienceOn
5 /
[ Sambrook,J.;E.F.Fritsch;T.Maniatis. ] / Molecular cloning: a laboratory manual.(2nd Ed.)
6 Taxonomic studies on Alternaria in Korea. /
[ Cho,H.S.;B.R.Kim;S.H.Yu. ] / Mycobiol
7 Alternarai themes and variation. /
[ Simmons,E.G. ] / Mycotaxon
8 Isolation and purification of an antitumor metabolite from Alternaria brassicicola SW-3,the cause of Brassica black leaf spot disease. /
[ Na,Y.J.;B.S.Lee;S.K.Namgoong;D.S.Jung. ] / Kor.J. Microbiol.Biotechnol.   과학기술학회마을
9 Alternaria toxins and their importance in food. /
[ King,A.D.Jr.;J.E.Shade. ] / J. Food Prot.
10 Phylogeny of Alternaria fungi kniwn to produce host-specific toxins on the basis of variation in internal transcribed spacers of ribosomal DNA. /
[ Kusaba,M.;T.Tsuge. ] / Curr,Genet.   ScienceOn
11 Species of Alternaria on Brassicas. /
[ Wiltshire.S.P. ] / CMI.Mycol.Papers
12 /
[ Omura,S. ] / The search for bioactive compounds from micro-organisms.
13 The impact of molecular characters on systematics of filamentous ascomycetes. /
[ Samuels,G.J.;K.A.Seifert. ] / Ann. Rev.Phytopathol.   ScienceOn
14 Fungal molecular systematics. /
[ Bruns,T.D.;T.J.White;J.W.Taylor. ] / Ann.Rev. Ecol.Syst.   ScienceOn
15 A hemorrhagic fator(Apicidin) produced by toxic Fusarium isolates from soybean seeds. /
[ Park,J.S.;K.R.Lee'J.C.Kim;S.H.Lim;J.A.Seo;Y.W.Lee. ] / Appl. Environ.Microb.
16 Antiplasmodial compounds from the wood-decayed fungus Xylaria sp. BCC 1067. /
[ Isaka,M.;A.Jaturapat;W.Kladwang;J.Punya;Y.Lertwerawat;M.Tanticharoen;Y.Thebtaranonth. ] / Plants Med,   ScienceOn
17 An antibiotic complex from Alternaria brassicicola. /
[ Ciegler,A.;L.Lindenfelser. ] / Experientia
18 Variation in the ITS and IGS regions of ribosomal DNA among the biological species of European Armillaria. /
[ Chillali.M.;H.Ladder-Ighili;J.J.Guillaumin;C.Mohammed;B.Lung Escarmant;B.Botton. ] / Mycol.Res.   ScienceOn
19 Cytochalasin E, an epoxide containing Aspergillus-derived fungal metabolite, inhibits angiogenesis and tumor growth. /
[ Udagawa,T.;J.Yuan;D.Panigraphy;Y.H.Chang;J.Shah;R.J.D’Amato. ] / J.Pharmacol Exp.Ther.
20 Components from the phytotoxic extact of Alternaria brassicicola, a black spot pathogen of canola. /
[ MacKinnon,S.L.;P.Keifer;A.A.William ] / Phytochem.   ScienceOn