• Title/Summary/Keyword: Trematoda

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Some Enzymes of Tricarboxylic Acid Cycle and Metabolites of Carbohydrate Metabolism in Adult Isoparorchis hypselobagri(Digenea: Trematoda) During in vitro Starvation

  • Bera, Bireshwar;Manna, Buddhadeb
    • International Journal of Industrial Entomology and Biomaterials
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    • v.18 no.2
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    • pp.91-95
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    • 2009
  • The presence of considerable amount of enzymes of TCA cycle isocitrate dehydrogenase (ICDH-NADP+, EC1.1.1.42), $\alpha$-ketogluterate dehydrogenase ($\alpha$-KGD, EC1.2.4.2) and malate dehydrogenase (MDH, EC1.1.1.37) in fresh control and in vitro starved adult Isoparorchis hypselobagri establish the functional TCA cycle in this fluke. The major metabolic end products are pyruvate, lactate, oxaloacetate and malate. The ratio of oxaloacetate/malate assess that oxaloacetate is reduced to malate and in this fluke the reverse TCA cycle is active. The pyruvate/lactate ratio shows pyruvate is reduced to lactate and the fluke is homolactate farmenters.

Biomphalaria glabrata (Pulmonata: Planorbidae): A Potential Second Molluscan Intermediate Host of A Human Intestinal Fluke, Echinostoma cinetorchis (Trematoda: Echinostomatidae)

  • Chung, Pyung-Rim;Younghun Jung;Joo, Chong-Yoon
    • The Korean Journal of Malacology
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    • v.17 no.1
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    • pp.57-61
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    • 2001
  • The present study examines the potential involvement of Biomphalaria glabrata, a known molluscan intermediate host of Schistosoma mansoni, in the life cycle of Echinostoma cinetorchis, one of the echinostomes that are ubiquitous parasites of vertebrates and are of importance in human and veterinary medicine and wildlife diseases. Echinostomes can be maintained easily and inexpensively in the laboratory and provide good models for biological research ranging from the molecular to the organismal. In the present study, no echinostome cercariae were released from the B. glabrata experimentally infected with E. cinetorchis miracidia, whereas all the Biomphalaria snails infected with E. cinetorchis cercariae were found to be infected with the metacercariae. This is the first report that B. glabrata can experimentally serve as the second intermediate host of E. cinetorchis, and that it might be employed as one of the target molluscs for establishing a biological research model with E. cinetorchis in the laboratory.

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Molecular phylogenie location of the Plagiorchis muris(Digenea, Plagiorchiidae) based on sequences of partial 28S D1 rDNA and mitochondrial cytochrome C oxidase subunit I

  • Lee, Soo-Ung;Huh, Sun;Sohn, Woon-Mok
    • Parasites, Hosts and Diseases
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    • v.42 no.2
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    • pp.71-75
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    • 2004
  • To determine the molecular phylogenie location of Plagiorchis muris, 28S D1 ribosomal DNA (rDNA) and mitochondrial cytochrome C oxidase subunit I (mtCOI) were sequenced and compared with other trematodes in the family Plagiorchiidae. The 28S D1 tree of P. muris was found to be closely related to those of P. elegans and other Plagiorchis species. And, the mtCOI tree also showed that P. muris is in a separate clade with genus Glypthelmins. These results support a phylogenie relationship between members of the Plagiorchiidae, as suggested by morphologic features.

In vitro excystation of metacercarial cysts of Echinostoma trivolvis from Rana species tadpoles

  • Fried, Bernard;Bradford, J.-David
    • Parasites, Hosts and Diseases
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    • v.35 no.2
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    • pp.75-78
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    • 1997
  • In uitro excystation studies were done on the metacercarial cysts of Echinostomn triuolvis obtained from the kidneys of naturally infected Rnna species tadpoles. Cysts were excysted in an alkaline trypsin-bile salts medium and the percentage of excystation was compared with that from previous studies done on cysts obtained from the kidneys of snails. The percentage of excystation of E. triuoluis metacercariae from tadpole kidneys was similar to that reported for previous studies on cysts obtained from experimentally infected gastropod hosts. The possible role of tadpoles as an agent for the transmission of Echinostomn and echinostomiasis to humans is discussed.

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Corbicula fluminea (Bivalvia: Corbiculidae): a possible second molluscan intermediate host of Echinostoma cinetorchis (Trematoda: Echinostomatidae) in Korea (한국산 "재첩"이 "이전고환극구흡충" 의 제 2 패류중간숙주가 될 수 있는가)

  • Chung, Pyung-Rim;Soh, Chin-Thack;Younghun Jung;Hwang, Myung-Gi
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2000.05a
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    • pp.502-502
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    • 2000
  • Corbicula fluminea Muller 1884 is the commonest freshwater bivalve in Asian countri including Korea. This clam is easily found in the freshwater systems, and edible as a food source in Korea. This study was aimed to confirm natural infection of C. fluminea collected from various localities with Echinostoma cinetorchis, an important hum intestinal fluke in Korea. (omitted)

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Benedenia derzhavini (Trematoda : Monogenea) from Cultured Korean Rockfish, Sebastes schlegeli, in Korea (양식 조피볼락에서의 Benededia derzhavini 기생에 관한 국내 보고)

  • Kim, Ki-Hong;Kwon, Se-Ryun
    • Journal of fish pathology
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    • v.11 no.1
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    • pp.83-86
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    • 1998
  • The benedeniine monogenean Benedenia derzhabini (Layman, 1930) Meserve, 1938 from cultured Korean rockfish, Sebastes schlegeli, is described and reported for the first time in Korea. The parasite was recovered from the gills and inner wall of operculum. B. derzhavini is distinguished from B. seriolae and B. sebastodis by the relative shape and length between the accessory sclerites and the hamuli.

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Trematodes of the family Opisthorchiidae: a minireview

  • King, Sandie;Scholz, Tomas
    • Parasites, Hosts and Diseases
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    • v.39 no.3
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    • pp.209-221
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    • 2001
  • Examination of the original descriptions of genera placed within the family Oposthorchiidae has revealed that only 33 of the original 43 genera are valid members of this family Further study of these descriptions should also reveal that many of the subfamilies are also invalid. With reference to the original descriptions of these genera, and subsequent literature, a brief survey of the family has been compiled. Information on the spectrum of definitive hosts that these helminths parasitize is provided, as well as information on the life cycles, geographic distribution, and socioeconomic impacts of the family. More in-depth information is given on those species that are of particular medical importance; namely, Clonorchis sinensis, Opisthorchis viverrini and O. felineus. The final aims of this review are to provide information on the entire genera of the family Opisthorchiidae, which will aid understanding of the phylogenetic relationships not only within the family, but also within the Class Trematoda.

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Austropeplea ollula (Pulmonata: Lymnaeidae): A new molluscan intermediate host of a human intestinal fluke, Echinostoma cinetorchis (Trematoda: Echinostomatidae) in Korea

  • Chung, Pyung-Rim;Jung, Young-Hun;Park, Yun-Kyu;Hwnag, Myung-Ki
    • Parasites, Hosts and Diseases
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    • v.39 no.3
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    • pp.247-253
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    • 2001
  • Three freshwater snail species of the family Lymnaeidae have been reported from Korea, Radix auricularia coreana, Austropeplea ollula and Fossaria truncatula. Out of 3 lymnaeid snail species, A. ollula was naturally infected with the Echinostoma cinetorchis cercariae (infection rate : 0.7%). In the experiments with the laboratory-bred snails, F. truncatula as well as A. ollula was also susceptible to the E. cinetorchis miracidia with infection rates of 25% and 40%, respectively. All of three lymnaeid snail species exposed to the E. cinetorchis cercariae were infected with the E. cinetorchis metacercariae. It is evident that A. ollula acts as the first molluscan intermediate host of E. cinetorchis in Korea, and F. truncatula may be a possible candidate for the first intermediate host of this intestinal fluke. Also, three lymnaeid snail species targeted were experimentally infected with E. cinetorchis metacercariae.

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The first human case of Clinostomum complanatum (Trematoda: Clinostomidae) infection in Korea (새인두흡충(Clinostomum complanatum)에 의한 인두염 제1례)

  • 정동일;문주환
    • Parasites, Hosts and Diseases
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    • v.33 no.3
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    • pp.219-224
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    • 1995
  • The authors present the first human case of Clinostomum pharyngitis in Taegu, Korea. The patient was a 56-year old male who visited an otolarlngology clinic due to foreign body sensation and pain of the pharyngeal region for 3-4 days. He used to eat raw fresh-water fish. Otolaryngological examinations revealed a living worm adhered to the right posterior pharyngeal wall. The worm removed was identified as C. complonotum after morphological observations. It is likely that more attention should be paid to eating raw fresh-water fish in Korea siC regards to Clinostomunl pharyngitis.

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Chromosomes of the liver fluke, Clonorchis sinensis

  • Park, Gab-Man;Im, Kyung-Il;Huh, Sun;Yong, Tai-Soon
    • Parasites, Hosts and Diseases
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    • v.38 no.3
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    • pp.201-206
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    • 2000
  • A karyological study was carried out in order to compared the chromosome numbers, chromosome morphologies and karyotypes of the oriental liver fluke, Clonorchis sinensis (Trematoda: Opisthorchiidae), collected from Korea and China. Chromosome preparations were made by means of air-drying method. The chromosome number was 2n=56 in both Korean and Chinese flukes, and chromosomes were divided into two groups based on this size; consisting of 8 pairs of large and 20 pairs of small chromosomes. However, the karyotypes showed some differences between Korean and Chinese flukes. The karyotype of liver flukes from Korea consisted of three metacentric pairs, one meta-/submetacentric pair, 16 submetacentric pairs and eight subtelocentric pairs of chromosomes. On the other hand, liver flukes from China consisted of two metacentric pairs, two meta-/submetacentric pairs, 16 submetacentric pairs and eight subtelocentric pairs of chromosomes.

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