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Some Enzymes of Tricarboxylic Acid Cycle and Metabolites of Carbohydrate Metabolism in Adult Isoparorchis hypselobagri(Digenea: Trematoda) During in vitro Starvation  

Bera, Bireshwar (Department of Zoology, University of Calcutta)
Manna, Buddhadeb (Department of Zoology, University of Calcutta)
Publication Information
International Journal of Industrial Entomology and Biomaterials / v.18, no.2, 2009 , pp. 91-95 More about this Journal
Abstract
The presence of considerable amount of enzymes of TCA cycle isocitrate dehydrogenase (ICDH-NADP+, EC1.1.1.42), $\alpha$-ketogluterate dehydrogenase ($\alpha$-KGD, EC1.2.4.2) and malate dehydrogenase (MDH, EC1.1.1.37) in fresh control and in vitro starved adult Isoparorchis hypselobagri establish the functional TCA cycle in this fluke. The major metabolic end products are pyruvate, lactate, oxaloacetate and malate. The ratio of oxaloacetate/malate assess that oxaloacetate is reduced to malate and in this fluke the reverse TCA cycle is active. The pyruvate/lactate ratio shows pyruvate is reduced to lactate and the fluke is homolactate farmenters.
Keywords
Adult Isoparorchis hypselobagri; In vitro starvation; TCA cycle enzymes; Metabolites; Pyruvate/lactate; Oxaloacetate/malate ratio;
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