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Identification and Characterization of Paraconiothyrium brasiliense from Garden Plant Pachysandra terminalis (가든식물 수호초(Pachysandra terminalis)로부터 Paraconiothyrium brasiliense의 분리 및 동정)

  • Choi, Min Ah;Park, Seung Jun;Ahn, Geum Ran;Kim, Seong Hwan
    • The Korean Journal of Mycology
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    • v.42 no.4
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    • pp.262-268
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    • 2014
  • A fungal isolate DUCC5000 from a garden plant Pachysandra terminalis was identified as Paraconiothyrium brasiliense based on the results of morphological and molecular studies. The fungus formed brown to black conidiomata of (0.2-0.7)-2(-3.5) mm singly or as a group on PDA. Conidia measured $2-5{\times}1.8-3{\mu}m$ in size, hyaline, ellipsoid to short-cylindrical, and rounded at both ends. The internal transcribed spacer (ITS) DNA of the isolate shared 100% nucleotide sequence homology with those of known P. brasiliense isolates. Phylogenetic tree inferred from the ITS sequence analysis showed that the DUCC5000 isolate formed a clade with known isolates of P. brasiliense. The fungal mycelia grew better on oatmeal agar than on MEA and PDA. On PDA media under various pH conditions, fungal mycelial growth was observed at pH 9. Colony morphology of the fungus tended to alter depending on the kinds of nutrient media and pH condition. On chromagenic media, the fungus demonstrated its ability to produce extracellular enzymes including amyalse, avicelase, ${\beta}$-glucosidase, protease, and xylanase. However, in pathogenicity testing, no disease symptoms were observed on the leaves of P. terminalis. This strain is the first report on P. terminalis in Korea.

Enhancement of Anticancer Activities of Ephedra sinica Stapf Extracts by Nano-encapsulation (마황 추출물의 나노 입자화를 통한 항암 활성 증진)

  • Jeong, Hyang-Suk;Kim, Seoung-Seop;Oh, Sung-Ho;Jeong, Myoung-Hoon;Choi, Woon-Yong;Seo, Yong-Chang;Na, Chun-Soo;Kwak, Hyeong-Geun;Lee, Hyeon-Yong
    • Korean Journal of Medicinal Crop Science
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    • v.18 no.3
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    • pp.143-150
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    • 2010
  • This study showed the increase of antitumor activities of water soluble E. sinica extract by nano-encapsulation process with lecithin. Five groups of lecithin only group (LO), lecithin nano-encapsulated E. sinica group (LE), E. sinica only group (EO), one negative control group (NCO) and positive control group (PCO) were set for several anticancer experiment and fed into Sarcoma-180 injected mice. The cytotoxicity of LE on the human normal kidney cell (HEK293) showed 14.8% lower than 19.2% of EO and 18.4% of LO. Growth of human liver carcinoma cell and human stomach carcinoma cell as representative of digestive system in vitro was inhibited up to about 85.1% and 87.3%, in adding 1.0 mg/$m{\ell}$ of LE, which values 15% higher than that from conventional EO. The survival rates of each mice group were 40%, 63%, 48%, 33% and 100%, respectively after 40 days of injecting Sarcoma-180. The increment of their body weights of the extract feeding groups was suppressed down to 10~15%, compared to the negative control. The nano-particles also reduced the hypertrophy of the internal organs such as spleen and liver down to 15~20%, compared to those as the other groups. Among them, LE effectively reduced the size of tumor form to 20%. From these results, in vitro and in vivo antitumor activities of E. sinica could be enhanced by using nano-encapsulation process with lecithin because of better permeation into the cancer cells by confocal observations.

A Study on the Natural Interspecific Hybrid between Rhodeus notatus and R. ocellatus (떡납줄갱이 Rhodeus notatus와 흰줄납줄개 R. ocellatus의 자연 종간잡종에 관한 연구)

  • Yun, Bong Han;Sung, Mu Sung;Kim, Yong Hwi;Bang, In-Chul
    • Korean Journal of Ichthyology
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    • v.33 no.3
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    • pp.157-166
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    • 2021
  • Three specimens, presumed to be natural hybrids between Rhodeus notatus and R. ocellatus, were collected from Onyangcheon Stream area, Jwabu-dong, Asan-si, Chungcheongnamdo, the Republic of Korea, and morphological and phylogenetic analyses were performed to clearly identify their parent species. The body color of the three natural hybrids was light greenish-brown on dorsal side, and the size of red area on the upper front of the dorsal fin and the outer margin of the anal fin generally showed intermediate characters between the parent species, R. notatus and R. ocellatus. Among the measurement and meristic characters, the ratio of prepectoral length and preanal length in the standard length, and the ratio of snout length, interorbital width, length of caudal peduncle and depth of caudal peduncle in the head length, and the number of longitudinal row scales were analyzed as the unique characters of natural hybrids. In the rag1 gene of nuclear DNA, the three natural hybrids were analyzed to be reflected all the single nucleotide polymorphism sites between R. notatus and R. ocellatus, and in the phylogenetic tree using the cytb gene of mitochondrial DNA, they formed the same genetic clade as R. notatus. Therefore, three specimens, presumed to be natural hybrids analyzed in this study were identified as interspecific hybrids between female R. notatus and male R. ocellatus.

Foraging behavior and pollination efficiency of honey bees (Apis mellifera L.) and stingless bees (Tetragonula laeviceps species complex) on mango (Mangifera indica L., cv. Nam Dokmai) in Northern Thailand

  • Chuttong, Bajaree;Panyaraksa, Lakkhika;Tiyayon, Chantaluk;Kumpoun, Wilawan;Chantrasri, Parinya;Lertlakkanawat, Phurichaya;Jung, Chuleui;Burgett, Michael
    • Journal of Ecology and Environment
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    • v.46 no.3
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    • pp.154-160
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    • 2022
  • Background: The mango is one of the essential fruit trees for the economy of Thailand. Mango pollination relies primarily on insects. Other external forces, such as wind, are less efficient since pollen is sticky and aggregating. There is only one report from Thailand on the use of bees as mango pollinators. The study of the behavior and pollination efficiency of honey bees (Apis mellifera) and stingless bees (Tetragonula laeviceps species complex) was conducted in Nam Dokmai mango plantings in Phrao and Mae Taeng districts, Chiang Mai province, between February and March 2019. Results: Our results reveal that the honey bees commenced foraging earlier than the stingless bee. The number of flowers visited within 1 minute by honey bees was higher than that visited by stingless bees. The average numbers of honey bees and stingless bees that flew out of the hive per minute from 7 a.m. and 6 p.m. in the Phrao district were 4.21 ± 1.62 and 9.88 ± 7.63 bees/min, respectively, i.e., higher than those observed in Mae Taeng, which were 3.46 ± 1.13 and 1.23 ± 1.20 bees/min, respectively. The numbers of fruits per tree were significantly higher in the honey bee and stingless bee treatments (T1 and T2) than in the open pollination treatment (T3). The number of fruits between T1 and T2 treatments was not different. In the pollinator exclusion treatment (T4), no fruit was produced. Fruit size factors were not significantly different among T1, T2, and T3 treatments. Conclusions: Our results showed that insect pollination is crucial for mango production, especially with the Nam Dokmai variety in Northern Thailand. As pollinator exclusion treatment showed no fruit set, and pollinator treatment significantly increased the fruit sets compared to open access plots, a managed pollinator program would benefit the mango growers for better productivity. Both the honey bee and the stingless bee were shown to be effective as pollinators.

Managing the Reverse Extrapolation Model of Radar Threats Based Upon an Incremental Machine Learning Technique (점진적 기계학습 기반의 레이더 위협체 역추정 모델 생성 및 갱신)

  • Kim, Chulpyo;Noh, Sanguk
    • The Journal of Korean Institute of Next Generation Computing
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    • v.13 no.4
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    • pp.29-39
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    • 2017
  • Various electronic warfare situations drive the need to develop an integrated electronic warfare simulator that can perform electronic warfare modeling and simulation on radar threats. In this paper, we analyze the components of a simulation system to reversely model the radar threats that emit electromagnetic signals based on the parameters of the electronic information, and propose a method to gradually maintain the reverse extrapolation model of RF threats. In the experiment, we will evaluate the effectiveness of the incremental model update and also assess the integration method of reverse extrapolation models. The individual model of RF threats are constructed by using decision tree, naive Bayesian classifier, artificial neural network, and clustering algorithms through Euclidean distance and cosine similarity measurement, respectively. Experimental results show that the accuracy of reverse extrapolation models improves, while the size of the threat sample increases. In addition, we use voting, weighted voting, and the Dempster-Shafer algorithm to integrate the results of the five different models of RF threats. As a result, the final decision of reverse extrapolation through the Dempster-Shafer algorithm shows the best performance in its accuracy.

Real-Time Terrain Visualization with Hierarchical Structure (실시간 시각화를 위한 계층 구조 구축 기법 개발)

  • Park, Chan Su;Suh, Yong Cheol
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.29 no.2D
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    • pp.311-318
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    • 2009
  • Interactive terrain visualization is an important research area with applications in GIS, games, virtual reality, scientific visualization and flight simulators, besides having military use. This is a complex and challenging problem considering that some applications require precise visualizations of huge data sets at real-time rates. In general, the size of data sets makes rendering at real-time difficult since the terrain data cannot fit entirely in memory. In this paper, we suggest the effective Real-time LOD(level-of-detail) algorithm for displaying the huge terrain data and processing mass geometry. We used a hierarchy structure with $4{\times}4$ and $2{\times}2$ tiles for real-time rendering of mass volume DEM which acquired from Digital map, LiDAR, DTM and DSM. Moreover, texture mapping is performed to visualize realistically while displaying height data of normalized Giga Byte level with user oriented terrain information and creating hill shade map using height data to hierarchy tile structure of file type. Large volume of terrain data was transformed to LOD data for real time visualization. This paper show the new LOD algorithm for seamless visualization, high quality, minimize the data loss and maximize the frame speed.

Application of Fire Risk and Fire Risk Rating Assessment for Four Species of Wood According to Chung's Equation-XII (Chung's Equation-XII에 의한 목재 4종의 화재위험성 및 화재위험성 등급 평가 적용)

  • Yeong-Jin Chung;Eui Jin
    • Applied Chemistry for Engineering
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    • v.35 no.1
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    • pp.29-36
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    • 2024
  • The fire risk and fire safety of four types of wood were comprehensively evaluated according to Chung's equation-XII. White ash, willow, fraxinus mandshurica, and sagent cherry trees were selected as test specimens. A cone calorimetery (ISO 5660-1) was used to examine the combustion characteristics of the test piece, and finally, the fire risk rating (FRR) was predicted using the fire risk index-XII (FRI-XII). The predicted fire performance index-X (FPI-X) and fire growth index-X (FGI-X) ranged from 469.03 to 1109.73 s2/kW and 0.0009 to 0.0280 kW/s2, respectively. Additionally, the fire performance index-XI (FPI-XI) and fire growth index-XI (FGI-XI) ranged from 0.41 to 0.97 and 1.11 to 3.11, respectively. The fire risk index-XII (FRI-XII), representing a fire risk rating, showed that the fire risk of frasxinus mandsurica tree (FM) was very high at 7.60 (fire risk rating: D). And it was compared with Chung's equation-IX, fire risk index-IX (FRI-IX). The fire risk ratings according to FRI-IX and FRI-XII were generally high for willow and frasxinus mandsurica trees. Additionally, the results of FRI-XII and FRI-IX had a similar relationship, and the size of each fire safety rating closely matched each other.

Vegetation structure and environmental factors in Paksil-nup Wetland, Hapcheon (박실늪의 식생 구조와 환경 요인)

  • Kim, Cheol-Soo;Lee, Pal-Hong;Son, Sung-Gon;Oh, Kyung-Hwan
    • Journal of Wetlands Research
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    • v.2 no.1
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    • pp.31-40
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    • 2000
  • The flora, vegetation structure and physico-chemical characteristics of surface sediment were investigated in Paksil-nup wetland of Hapcheon-gun, Gyeongsangnam-do, Korea in 1990~1998. Ranges of the surface sediment characteristics such as pH, conductivity, organic matter, total nitrogen, available phosphorus, exchangeable K, exchangeable Ca, exchangeable Mg, and exchangeable Na were 4.36~4.34, $19.0{\sim}1260.0\;{\mu}mho\;cm^{-1}$, 0.01~6.35%, 0.001~0.14%, 0.01~0.31 mg/l00g, 1.01~13.98 ppm, 16.75~143.80 ppm, 0.93~14.85 ppm and 0.21~3.86 ppm, respectively. Percentages of the particle size such as sand, silt, and clay were 13.0~93.3%, 5.4~71.7%, and 0.5~37.5%, respectively. The flora of the study area was composed of 72 families, 182 genera, 223 species, 36 varieties and 2 form or total 261 kinds, and those were 45 kinds of vascular hydrophytes and 216 kinds of vascular hygrophytes. The life form of vascular hydrophytes was classified as 27 kinds (60%) of emergent plants, 8 kinds (18%) of submerged plants, 6 kinds (13%) of free-floating plants and 4 kinds (9%) of floating-leaved plants, respectively. The importance value of Salix nipponica was highest as 123.78 in the shrub and tree layers, and that of Trapa japonica was highest as 16.69 in the herb layer. The vegetation type was divided into two groups according to the association analysis. The vegetation of the littoral zone was classified into 7 associations according to the cluster analysis based on the coverage data. These results showed significant differences with those of stand ordination by correspondence analysis based on the species composition and by PCA based on the sediment properties. Factors affecting the distribution of the vascular hydrophytes and hygrophytes were the gradient of particle size, altitude, and water depth.

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Influences of Environmental Gradients on the Patterns of Vegetation Structure and Tree Age Distribution in the East Side of Cascade Range, Washington, USA (워싱턴주(州) 케스케이드산맥(山脈) 동(東)쪽 산림(山林)에서 환경구배(環境勾配)가 식생구조(植生構造)와 연령분포(年齡分布)에 미치는 영향(影響))

  • Woo, Su Young;Lee, Kyung Joon;Lee, Sang Don
    • Journal of Korean Society of Forest Science
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    • v.85 no.1
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    • pp.107-119
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    • 1996
  • To understand vegetation changes along environmental gradients in the natural forests in the east side of the Cascade Range in Washington state, USA, line transects were used to sample six different forest environments in the Wenatchee National Forest in the north-facing and south-facing sites at 975, 1280 and 1700m elevation. Data were analyzed using ordination by detranded correspondence analysis. Pseudotsuga menziesii was found as one of the dominant species on all the six sites regardless of elevation or aspect, while Pinus ponderosa was dominant on south slopes only. Abies grandis and A. lasiocarpa were dominant species on north slopes at elevations of 1280 and 1700m, respectively. Moisture, as it related to aspect, was identified as one of the most important environmental gradients for explaining the variation of vegetation types. On north-facing slopes, compared to south-facing slopes, where moisture was not as limiting and canopies could grow denser, probably, elevation or competitive interaction was more important. Species diversity tended to decrease with increasing environmental severity, with south slopes having less diversity than north slopes due to extended water stress and harsher temperature extremes on south slopes. The age structure on north-facing and south-facing slopes was different. Light intensity, moisture and climate were different between these two slopes. Large scale disturbances(e.g., big fire or insects) were major causes in changing age structure. Younger trees showed a closer relationship between size and age than adult trees. DBH values of shade intolerant species in south-facing slope were bigger than those of north-facing slope, which suggested that aspect of stands be the most important factor for age and size.

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Responses of Native Trees Species in Korea under Elevated Carbon Dioxide Condition - Open Top Chamber Experiment (상부 개방형 온실을 이용한 대기 중 이산화탄소 농도 증가가 우리나라 자생 수종에 미치는 형태적, 생리적 영향)

  • Ryu, Daun;Bae, Jinho;Park, Juhan;Cho, Sungsik;Moon, Minkyu;Oh, Chang-Young;Kim, Hyun Seok
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.16 no.3
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    • pp.199-212
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    • 2014
  • The physiological responses of three common temperate species, Pinus densiflora, Fraxinus rhynchophylla, Sorbus alnifolia to elevated $CO_2$ was investigated using open top chambers with different $CO_2$ concentrations. Morphological (stomatal size, density and area) and physiological characteristics (maximum rates of photosynthesis, carboxylation and electron transport) were compared among trees grown under ambient, ambient ${\times}1.4$ (~550 ppm) and ambient ${\times}1.8$ (~700 ppm) $CO_2$ concentrations for last four years. Morphological responses were different among species. F. rhynchophyllar increased their stomatal size and S. alnifolia had higher stomatal density under elevated $CO_2$ than ambient. Stomatal area decreased in P. densiflora, whereas it increased in S. alnifolia. However, the maximum photosynthesis rate increased in all species up to 43.5% by S. alnifolia under elevated $CO_2$ and the enhancement increased with time. Even with four years of exposure to elevated $CO_2$, there was no sign of acclimation in the maximum carboxylation rate and the maximum electron transport rates in all species. Especially, S. alnifolia even showed the temporary increase of photosynthetic capacities in spring, when leaf nitrogen concentration was high with new leaf development. There was no significant differences in diameter growth rate in any species due to high variation in their tree sizes, however accumulated diameter and biomass for four years showed significantly increment in all species under elevated $CO_2$. For example, S. alnifolia showed 59% increase in diameter at the ambient ${\times}1.8$ (~700 ppm) compared to ambient.