• 대한수의학회지
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• 제36권4호
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• pp.941-948
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• 1996

The accuracy of rectal palpation and ultrasonography for predicting the presence of a functional corpus luteum in subestrous dairy cows was investigated, using the result of a radioimmunoassay for progesterone in plasma. Luteal status (high or low progesterone concentrations) was diagnosed in 820 cows, using rectal palpation and B-mode transrectal ultrasonography, and the results of rectal palpation and ultrasonography were compared in $2{\times}2$ contingency table with plasma progesterone concentrations. A $2{\times}2$ contingency table analysis allowed the calculation of sensitivity, specificity and predictive values for rectal palpation and ultrasonography. The sensitivity, specificity, predictive value of a positive test and predictive value of a negative test were 81.9%, 67.5%, 79.0% and 71.4% for rectal palpation, and 96.3%, 88.8%, 94.5% and 92.4% for ultrasonography, respectively. The percentages of observed agreement and expected agreement between rectal palpation and ultrasonography were 71.8% and 57.1%, respectively. An evaluation of agreement between rectal palpation and ultrasonography, the value of Kappa was 0.34. It was concluded that a ultrasonography was more sensitive and specific than rectal palpation in predicting the presence of a functional corpus luteum. Therefore, ultrasonographic examination is a reliable method for assessing the functional status of ovarian structures in subestrous dairy cows.

• Animal Bioscience
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• 제34권2호
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• pp.198-204
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• 2021

Objective: The aim of this study was to ascertain the effects of adding green tea extract (GTE) to skim milk-egg yolk (SM-EY) extender on both the quality of post-thawed bull semen and the pregnancy rates of the recipient cows. Methods: Twelve ejaculates from four Simmental bulls, aged 3 to 5 years and weighing 900 to 950 kg, were diluted SM-EY extender, added with 0, 0.05, 0.1, and 0.15 mg GTE/100 mL extender and then frozen. After four weeks storage in liquid nitrogen, the sperm were thawed and evaluated for viability, motility, intact plasma membrane (IPM), and DNA fragmentation. Meanwhile, the estrus cycles of 48 recipient cows were synchronized by intramuscular administration of a single injection of 5 mg prostaglandin F2α. Estrus cows were divided into four equal groups and inseminated artificially 18 to 20 h after the onset of estrus by using semen from each extender group. Pregnancy was diagnosed by measuring serum progesterone levels at 21 days, followed by transrectal palpation 90 days after insemination. Results: The findings revealed that adding 0.1 mg of GTE/100 mL extender produced the highest percentages of sperm viability (70.67%±1.75%), motility (69.17%±1.47%), and IPM (69.23%±1.21%) and the lowest percentage of DNA fragmentation (3.00%±0.50%). The pregnancy diagnosis revealed that all cows (36/36) inseminated using frozen semen in GTE addition extender were pregnant (pregnancy rate 100%), whereas the pregnancy rate of the control group was 83.33% (10/12). Conclusion: It may be concluded that 0.1 mg GTE/100 mL extender yields the best quality of spermatozoa and that all variants doses of GTE in extender produce a higher pregnancy rate among recipient cows.

• 한국임상수의학회지
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• 제15권2호
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• pp.307-318
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• 1998

Accuracy of rectal palpation and ultrasonography for differential diagnosis of subestrous dairy trows were investigatedl using the result of pIRsma progesterone assay. The ovaries were examined 2 times of 10 days interval in 520 posearom and postinsemination subestroHs dairy cows, using rectal palpation and B-mode transrectal ultrasonography. The results of rectal palpation, ultrasonographic examination and measurement of plasma progesterone profiles in 520 subestrous dairy cows were silent brat or error of estrus detection 303 (58.3%), persistent corpus luteum 59 (11.3%), follicular cyst 37 (7.1%), luteal cyst 16 (3.1%), inactive ovary 9 (1.7%), granulosa tumor 1 (0.2%), hydmsalphinx 1 (0.2%), endomehris 81 (15.6%), pyometra 12 (2.3%) and mummified fetus 1 (0.2%), respectively. Accuncy of rectal palpation and ultrasonography for diagiosing ovarian disordeir based on plasma progesterone profiles were silent heat or error of estrus detection 80.5% and 96.7%$\boxUl$ persistent corpus luteum 57.6% and 94.9%, follicular cyst 62.5% and 91.9%1 luteal cyst 62.5% and 87.5%, maclive ovary 55.6% and 88.9% and granulosa cell tumor 100% and 100%, respectively. Acnuucy of rectal palpation for diagnosing uterine disorders based on ultrasonography was pyometra 75.0%1 endometritis 51.9% and mummified fetus 100%, respectively. Cbaracteristic ultrasonographic appearances of ovaries in subestrous dairy cows were as follows; Silent heat or error of estrus detection: anechoic follicle or hypoechoic corpus luteum than ovarian stroma was alternately present on Day 0 (first examination) and Day 10. Follicular cyst: uniformly nonechogenic ovarian structure $\geq $ 25 mm in diameter with a wall < 3 mm was present in ipsilateral on Day 0 and Day 10. Luteal cyst: luteal cyst was similar to follicular cysts but thickness of cystic wall was $\geq $ 3 mm. Inactive ovary : structures within ovaries was not present on Day 0 Bnd Day 10. Characteristic uthssonograpsc appearances of uterus in subestrous dairy cows were as follows; Endometritis: characterized by uterine lumen containing fluid in which 'snowy'echogenic particles art suspended. Pyometra: ultrasonographic appearance of pyometra was diffuse echogenic particles distributed in fluid within the distended uterus, and a thickened uterine wall. These results indicated that ultrasonography was practical far diagnosing reproductive disorders. To diagnosing ovarian disorders, ultrasonography should be carried out 2 times of 10 days interval and rndometritis should be differentiated with uterus of luteal phase in normal cycling cows.

• 한국임상수의학회지
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• 제38권4호
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• pp.199-203
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• 2021

A 4-year-old Thoroughbred mare was brought to the Korea Racing Authority Equine Hospital with heightened sensitivity to touch of the hindquarter, reluctance to step forward, frequent urination even during training, clitoral winking, tail swinging, tail lifting, and training difficulties due to being in constant heat. The trainer claimed that she was in heat and exhibited reduced performance. Physical and lameness examinations revealed no abnormality. Rectal palpation and transrectal ultrasonography revealed normal reproductive organs and estrus. As she exhibited typical estrous behavior without any other physical conditions, the mare was considered to express undesirable estrous behavior. An intrauterine glass ball was inserted into the mare on ovulation day. However, the estrous behaviors were reduced unsatisfactorily. Additionally, 1 mL of peanut oil was administered on the 10th day after the intrauterine device insertion. The mare returned to training and expressed no undesirable estrous behavior over 30 days after the peanut oil treatment. However, further follow-ups were not conduct as the mare was retired to a farm after a limb injury occurred during training. This is the first report of undesirable estrous behavior in a Thoroughbred mare under the rules of racing in Korea. It is recommended that equine clinicians in Korea consider peanut oil as a therapeutic agent to control mares' estrous behavior-related problems.

• Asian-Australasian Journal of Animal Sciences
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• 제11권5호
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• pp.486-490
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• 1998

The present study examined the follicular populations prior to and during superovulation and investigated their relationship with superovulatory response in crossbred cattle. Eleven animals were administered i.m. 8 doses of Folltropin of 2.5 ml each (1.75 mg/ml) spread over 4 days beginning on Day 10 of oestrous cycle, and 30 and 20 mg Lutalyse was given alongwith the 5th and 6th injections of Folltropin, respectively, to induce luteolysis. The animals were artificially inseminated 48, 60 and 72 h after the first Lutalyse injection. The number of corpora lutea (CL) was recorded by palpation per rectum and embryos were recovered non-surgically on Day 6 (Day 0 day of superoestrus). The ovarian follicular population was examined by transrectal Ultrasonography 15 h prior to and 52 h after the first FSH injection, and then on the day of superoestrus and the day of flushing. The follicles were classfied on the basis of diameter as small (3-5 mm), medium (6-9 mm) and large (${\geq}10mm$). The total number of follicles increased significantly (p < 0.01) from $2.45{\pm}0.35$, 15 h prior to the first FSH injection to $8.09{\pm}1.12$, 52 h after the first FSH injection and then further to $13.27{\pm}1.89 $ on the day of superoestrus. A positive correlation was observed between the number of small follicles 15 h prior to the first FSH injection (r = 0.60, p < 0.05), the number of large follicles 52 h after the first FSH injection (r=0.59, p < 0.05) and the number of CL. The follicular population prior to and during superovulation was, however, not significantly different between high (> 6 CL) and low responders (${\leq}6CL$). The present study suggests that the follicular populations undergo dynamic changes during superovulation and that follicular populations prior to superovulation have a limited application as an indicator of the superovulatory response.

• 한국임상수의학회지
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• 제26권5호
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• pp.486-489
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• 2009

An 8-year-old Thoroughbred mare showing anestrus for longer than 6 months with left ovarian mass was referred to Veterinary Medical Teaching Hospital of Seoul National University. For 6 months, the mare had received 2 times hormonal therapies with $PGF_2{\alpha}$ and progesterone. The mare had not responded to these therapies and not shown estrous behavior. On rectal palpation, the left ovary was enlarged and contralateral ovary was small and inactive. Transrectal ultrasonographic examination of the left ovary revealed a mass composed of multiple, irregularly shaped follicles. Granulosa cell tumor (GCT) was suspected according to her history. Ovariectomy was performed through the left flank laparotomy with a standing position in a simple chute located outside of horse farm. The mass was diagnosed as GCT on histopathology. The mare was recovered without any problem and became pregnant next spring.

• 한국임상수의학회지
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• 제26권3호
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• pp.282-285
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• 2009

10살의 페인트종의 말이 수컷과 비슷하게 사람과 다른 말들에게 공격적인 양상을 점차적으로 보였다. 직장검사와 초음파 검사를 통하여 오른쪽 난소의 이상을 발견하였다. 혈액치와 혈액화학치는 정상이었으나, 테스토스테론(46.00 pg/mL), 인히빈(0.856 ng/mL,), 프로게스테론(1.2 ng/mL)은 정상수치보다 증가하였다. 비대해진 난소는 diagonal paramedian approach법으로 제거하였다. 이 방법은 수술부위를 적게 절개할 수 있으며, 출혈을 최소화할 수 있다. 수술 후 합병증 없이 행동적 이상을 보이지 않았으며, 호르몬 수치도 정상 수치를 보였다. 제거한 난소는 조직검사 결과 난소 섬유종으로 진단되었다.

• 현장농수산연구지
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• 제20권2호
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• pp.117-123
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• 2018

본 연구의 목적은 성숙한 당나귀의 큰잘록 창자 폐색 증례를 진단하여 보고하고자 한다. 당나귀(Equus africanus asinus; 암, 6세) 1마리가 제주경마공원 동물병원에 전날부터 시작된 식욕부진 증상으로 내원하였다. 장음소실, 탈수, 심한 복강 확장 등의 증상을 확인하였으나 호흡수 및 심박수는 정상이었고, 복통 증상은 없었다. 직장검사에서 폐색된 부분이 잘록 창자임을 확인하였다. 위세척, 수액요법 및 비스테로이드 계열의 진통제를 투여하였으나 다음날 새벽 갑자기 몸을 떨며 기립곤란과 같이 신체상태가 악화되기 시작한지 2시간 만에 폐사하였다. 부검에서 특히 왼배쪽 잘록 창자와 같은 큰잘록 창자에서 다량의 식괴를 발견하였고, 막창자와 곧창자는 정상이었다. 당나귀는 내원하기 11일 전에 새로 입사해서 사양 관리체계가 바뀌었다. 사료변경, 수분섭취 감소, 운동부족 등이 질병을 유발하였을 것으로 생각된다. 그러나 당나귀의 정확한 급사원인은 더 연구해야 할 과제로 남는다.

• 한국수정란이식학회지
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• 제33권3호
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• pp.149-157
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• 2018

The objective of this study was to evaluate the effect of treating dairy cattle with exogenous human chorionic gonadotrophin (hCG), five (5) days post artificial insemination (AI) on serum progesterone (P4) concentration and pregnancy rate. In this experiment, five days after AI, cows were assigned randomly to two groups namely: a) treated group (67) which were administrered with 1500 IU hCG (Chorulon) and b) control group (61), which received no treatment. On day 5, 10, 15 and 20 after the artificial insemination, blood samples from a total of 8 cows (4 from each group) were collected and were analyzed for serum P4 concentration. Cows were detected for estrus according to standing heat by visual observation. Cows that were detected still in estrus after days 18-24 were re-inseminated and recorded as not pregnant (open). Pregnancy diagnosis was conducted by ultrasonographic examination and transrectal palpation of the uterus on approximately 60 days in cows that observed to be not in estrus. The conception rate in hCG treated and control groups were 52.5 and 36.1%, respectively. The results proved that there were no significant differences in conception rate between two groups (p=0.0568). However, pregnancy rates were reduced by hCG treatment. Average serum P4 concentrations did not differ between Hcg-treated and control groups on day 5 (0.377 versus 0.375 ng/ml). On day 20 serum P4 concentrations were greater in the treated group compared with the control group (3.085 versus 2.010 ng/ml). The treatment with hCG seemed to increase P4 level compared with the control. In conclusion, the results of this study showed that 1500 IU of hCG administered on 5 day post AI increased conception rate in dairy cows. This was supported by the results on serum P4 concentration which was greater in hCG treated group.