• Journal of Korean Neurosurgical Society
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• 제30권4호
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• pp.417-424
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• 2001

Objective : Adrenal medullary chromaffin cells are known to release analgesic substances such as opioides and catecholamines. Transplantation of them is a novel method that challenges current approaches in treating chronic pain. The transplantation of xenogeneic chromaffin cells into the central nervous system(CNS) supply antinociception in animals. In this study, we investigated the analgesic effects of rat adrenal medullary chromaffin cells transplanted into the CNS of the mouse. To study the antinociceptive efficacy of transplanted chromaffin cells, the survival of rat adrenal medullary chromaffin cells transplanted into the CNS of mouse was determined. Methods : The adrenal medullary chromaffin cells isolated from rat were transplanted into the striatum of mouse. These cells were confirmed of the release of Met-enkephalin and Leu-enkephalin by HPLC, and immunoblots for tyrosine hydroxylase(TH). Two weeks after transplantation, we performed immunohistochemistry for TH to determine the survival of implanted cells and assessed pain sensitivity at the same time. Results : The isolated rat adrenal medullary chromaffin cells were positive for anti-TH antibody and released Met-enkephalin and Leu-enkephalin more than rat endothelial cells. Transplanted rat chromaffin cells were stained with anti-TH antibody in striatum of mouse after 2 weeks. Pain sensitivity was reduced on the chromaffin cell-transplanted mouse compared to endothelial cell-transplanted mouse by the hot plate test. Conclusion : These results suggest that the rat chromaffin cells were suitably transplanted into the CNS of mouse. This approach could be used as a therapy for reducing of chronic pain induced by cancer or neuronal injury.

• 농업생명과학연구
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• 제50권4호
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• pp.59-71
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• 2016

논에서 이앙시기에 따른 화본과와 사초과 잡초의 발생 양상을 확인하고 벼에 대한 피해 정도를 알아보기 위해서 벼 이앙기를 4월 30일부터 6월 19일까지 10일 간격으로 달리하여 이앙 한 후 5월 20일부터 잡초발생과 벼의 생육을 확인하였다. 잡초들의 발생본수는 화본과와 사초과 잡초 모두 1차와 2차 이앙시기에 이앙한 시험구에서 발생본수가 많았으며, 크게 1, 2차 이앙구와 3, 4, 5, 6차 이앙시기 두 개의 그룹으로 나누어지는 결과를 보였다. 개체 당 건물중이 비교적 큰 피는 7월까지는 조기 이앙한 경우 건물중이 큰 값을 보였으나 8월 이후에는 만기 이앙한 시험구에서 건물중이 더 큰 값을 보였다. 반면 사초과 잡초는 지속적으로 빨리 이앙한 경우가 늦게 이앙한 경우보다 건물중이 높은 값을 보였다. 논의 써레질 시기가 빨라짐에 따라 조기에 잡초가 발생하고 높은 잡초생장률을 보이며 잡초의 경합력이 커져 벼의 생육과 수량이 줄어들었다. 잡초생장율을 기준으로 볼 때 화본과는 사초과 잡초에 비해 출수기 이후에 미치는 영향이 큰 것으로 확인되었다.

• Journal of Periodontal and Implant Science
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• 제23권2호
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• pp.261-281
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• 1993

The author transplanted periodontally-diseased teeth which had been treated with citric acid into a clinically healthy extraction sockets and periodontally-affected extraction sockets, and compared with the healing processes within these tissues. Recipient sites were prepared by surgically removing a part of alveolar bone of premolars of adults dogs, placing elastic orthodontic ligatures for 8weeks, thereby inducing periodontal disease. The diseased roots were extracted and transplanted into healthy extraction sockets, and these were designated as control group 1. Diseased roots transplanted into diseased sockets were designated as control group 2. Diseased roots which had been root planed, treated with citric acid and transplanted into healthy sockets were designated as experimental group 1, while identically treated roots which had been transplanted into diseased sockets were designated as experimental group 2. Observations were made at weeks 2, 8 and 12, with following results. 1. At week 2, experimental group 2 showed some inflammatory cell infiltration in the connective tissue above the extraction sockets, while control groups showed less inflammatory or foreign body reactions throughout the experiment. 2. In both control groups, root surface resorption was observed throughout the experiment, while experimental groups showed a little resorption. 3. Control group 1 & 2 showed ankylosis by newly-formed bone ground the resorbed root surfaces, while experimental group 1 & 2 displayed collagen fibers which are not functionally-arranged, with random, loose arrangement or parallel orientation to root surfaces, and newly-formed bone outside of them. 4. In both control groups & experimental groups which had been transplanted into a clinically healthy extraction sockets & periodontally affected extraction sockets groups, histological differences were not significant. 5. Root resorption or ankylosis in control group 1 & 2 had increased quantitatively as experiment progressed. 6. New bone formation developed from the base and lateral wall of extraction sockets. In both control groups & experimental groups, root surfaces lying next to the upper portion of extraction sockets showed little alveolar bone formation and surrounded by connective tissue fiber at weeks 2 & 8, while at weeks 12, they did show alveolar bone formation. 7. At week 12, experimental group 2 showed numerous cells which appeared to be periodontal ligament cells, with functionally arranged connective tissue fibers between the roots and alveolar bone.

• 한국작물학회지
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• 제57권3호
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• pp.233-237
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• 2012

강원도 조생종 쌀의 품질을 높이는 재배기술 개발을 목표로 강원도농업기술원 벼 시험포장에서 2년간 연구한 결과는 다음과 같았다. 1. 2010년 운광벼 쌀 수량은 5월 10일 이앙시 높았고, 이앙기가 늦어질수록 감소되었지만, 백미 완전미율은 이앙기가 늦어질수록 높아져서 완전미수량은 이앙기간에 차이가 적었다. 2. 2011년 운광벼는 6월 10일 이앙시 등숙율과 천립중 증가로 인하여 수량이 증가되어 재식거리와 재식본수 효과가 뚜렷이 나타나지 않았으나 $30{\times}13cm$-5본 이앙구에서 가장 많은 경향을 보였다. 3. 오래벼는 6월 10일 관행 재식거리와 본수로 이앙시 수량이 적어졌으나 $30{\times}13cm$-5본 이앙구에서는 $m^2$당 이삭수와 입수가 증가되어 관행재배법보다 5% 증수되었다. 4. 운광벼, 오래벼 모두 6월 10일에 이앙구에서 5월 25일 이앙구보다 등숙온도가 낮아져서 완전미율과 도요식 미치가 증가되는 등 품질이 크게 개선된 것으로 나타났다. 5. 본 연구결과를 응용하여 평야지 이외의 지역에서도 등숙온도가 $22^{\circ}C$에 가깝도록 이앙기를 늦추면서 재식거리를 좁히고 주당본수를 늘린다면 조생종 쌀 수량을 떨어뜨리지 않으면서 품질을 향상시킬 수 있을 것으로 기대된다.

• 대한한방부인과학회지
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• 제19권2호
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• pp.141-161
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• 2006

Purpose : This study was performed to investigate the effects of Sobokchukeo- Tang(SCT) on the experimentally-induced endometriosis in rats. Materials and Methods : Endometriosis was induced via the surgical autotransplantation technique in rats. A laparotomy was performed and a $4\;{\times}\;4\;mm$ of the right uterine horn was resected incised and sutured to the peritoneum. And the animals divided into control(n=8) and SCT-treated group(n=8). SCT(1,000 mg/head) was administered orally for 15 days after operation. The weights(body, left uterus, and ovaries) and concentrations of cytokines(MCP-1,$TNF-{\alpha}$, $IL-l{\beta}$) were measured. Histopathology, immunohistochemistry for COX-2, and histochemistry for mast cells of the transplanted uterine tissues were performed. Results : - The $volume(mm^3)$ of transplanted uterine tissues of SCT-treated group$(92.88{\pm}41.89)$ was significantly(p<<0.01) decreased than the control group $(404.50{\pm}317.68)$. The concentration(pg/ml) of MCP-1 in ascites of SCT-treated group$(5,256{\pm}1,209)$ was significantly(p<<0.001) decreased than the control group$(8,632{\pm}1,245)$. - The concentration(pg/ml) of $TNF-{\alpha}$ in ascites of SCT-treated group$(521.8{\pm}306.1)$ was significantly(p<<0.01) decreased than the control group$(1,245.2{\pm}362.2)$. - The percentage of COX-2 positive epithelial layer in transplanted uterine tissues of SCT-treated group$(25.0{\pm}7.3)$ was significantly(p<<0.001) decreased than the control group$(50.2{\pm}8.2)$. - The number of mast cells in the stroma of transplanted uterine tissues of SCT-treated group$(16.5{\pm}6.8)$ was significantly(p<<0.05) decreased than the control group$(26.0{\pm}7.7)$. - The number of mast cells in the periphery of transplanted uterine tissues of control group$(71.3{\pm}18.5)$ was significantly(p<<0.01) decreased than the control group$(109.3{\pm}30.2)$. - Proliferation of epithelia, infiltration of inflammatory cells, and microanglogenesis in transplanted uterine tissues of treated group were weakly observed than the control group. Conclusion : From the above results, Sobokchukeo-Tang(SCT) has an inhibitory effect on the development of transplanted uterine tissue in rats and it is related to the decreased concentration of MCP-1 and $TNF-{\alpha}$, and decreased expression of COX-2, and decreased infiltration of mast cells by administration of Sobokchukeo-Tang.

• 말소리와 음성과학
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• 제4권3호
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• pp.59-73
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• 2012

This study attempts to investigate the contribution of prosody to the foreign accent in Chinese speakers' English production by examining the synthesized speech of crossing native and non-native talkers' prosody and segments. For the stimuli of the foreign accent ratings, we transplanted gender-matched native speakers' prosody onto non-native talkers' segments and vice versa, utilizing the TD-PSOLA algorithm. Eight English native listeners participated in judging foreign accent and comprehensibility of the transplanted stimuli. Results showed that the synthesized stimuli were perceived as stronger foreign accent regardless of speakers' proficiency when English speakers' prosody was crossed with Chinese speakers' segments. This suggests that segments contribute more than prosody to native listeners' evaluation of foreign accent. When transplanted with English speakers' segments, Chinese speakers' prosody showed a difference in duration rather than pitch between high and low proficiency such that stronger foreign accent was detected when low proficient Chinese speakers' duration was crossed with English speakers' segments. This indicated that prosody, more specifically duration, plays a role though the prosodic role is not overall as significant as segments. According to the post acoustic analysis, the temporal features contributing to making the duration parameter prominent as opposed to pitch were found out to be speaking rate, pause duration and pause frequency. Finally, foreign accent and comprehensibility showed no significant correlation such that native listeners had no difficulty listening to highly foreign accented speech.

• 한방안이비인후피부과학회지
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• 제19권1호
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• pp.55-64
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• 2006

Objective : The purpose of this study was to investigate effect of Gungguitakli-San(GTS) on the anti-tumor, immunocytes. Methods : This study estimated the proliferation of L1210 and S-180 cell lines, mouse splenocytes and thymocytes in vitro, and estimated the proliferation of L1210 cell, S-180 cell, thymocytes and splenocytes and body weight in S-180 cells-transplanted mice. The cytotoxicity and proliferation of cells were tested using a colorimetric tetrazoliun assay(M1T assay). Results : The results of this study were obtained as follow ; 1. GTS was significantly increased in the proliferation of thymocytes and splenocytes In vitro. 2. GTS was significantly showed cytotoxicity on the L1210 cell lines and 8-180 cell lines in vitro. 3. GTS was significantly showed cytotoxicity on the L1210 cell lines in vivo. 4. GTS was significantly increased in the weight of mice and decreased weight of sarcoma, in S-180 cells transplanted mice. 5. GTS was significantly increased in the period of survive, in S-180 cells transplanted mice. Conclusions : The author thought that GTS had action of anti-cancer by becoming immunocytes activity and by cytotoxicity of cancer cells.

• 동의생리병리학회지
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• 제16권2호
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• pp.311-315
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• 2002

Cellular death by apoptosis is an active process, depending on gene transcription and protein synthesis. It was reported that nitric oxide can induce apoptosis in several cancer cell-lines. We studied effects of Phytolacca esculentum van Houtt (Phytolaccaceae) Radix water extract (PRE) on the proliferation of transplanted-L1210 cells in mice. When PRE (500 mg/kg) was administered orally once a day for 7 days after transplantation of L1210 cells to mice, DNA fragmentation of transplanted-L1210 cells was induced and mitochondrial transmembrane potential of those cells was reduced. Additionally, DNA fragmentation of L1210 cells was induced by the treatment of PRE in vitro. Also, DNA fragmentation of L1210 cells was enhanced by co-culture with the peritoneal macrophages obtained from PRE-administered mice and was partly inhibited by L-NMMA in vitro. PRE enhanced the production of nitric oxide and tumor necrosis factor-α from peritoneal rnacrophages. These results suggest that PRE induces apoptosis of transplanted-L1210 cells via directive action on L1210 cells and stimulation of nitric oxide and tumor neaosis factor-α from macrophages.

• 한국해양학회지
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• 제15권1호
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• pp.8-16
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• 1980

참굴, Crassostrea gigas(Thunberg)은 한국연안에 널리 분포되어 있는 종으로서 옛 부터 남, 서해안에서 송지식 투석식등으로 양식 해 왔고 현재에는 대형뗏목 및 연승을 이용한 굴양식이 집약적으로 기업화되고 있다. 굴에 관해서는 분류, 생리, 생태 및 양식시험등 각 분야에 걸쳐 많은 연구결과들이 있다.

• 생약학회지
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• 제29권2호
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• pp.110-119
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• 1998

Sa-Mul-Tang(SMT) consist of Rehmanniae Radix Preparata, Paeoniae Radix Alba, Cnidii Rhizoma and Angelicae Gigantis Radix. In L1210 cells-transplanted BALB/c mice, T-lymphocyte apoptosis, $CD8^+T_C$ cells population in thymocyte and nitric oxide production in macrophage were enhanced, but phagocytic activity was decreased. SMT suppressed T-lymphocyte apoptosis and enhanced CD^4+T_H$ cells population, but did not affect nitric oxide production and phagocytic activity in L1210 cells-transplanted mice. In antitumor drugs-injected mice, T-lymphocyte apoptosis was enhanced, but $CD4^+T_H/CD8^+T_C$, cells population and T-lymphocyte proliferation were decreased. SMT suppressed T-lymphocyte apoptosis, and enhanced $CD8^+T_C$ cells population, T-lymphocyte proliferation and phagocytic activity in vincristine-injected mice. These results suggest that SMT enhances T cell-mediated immunity in L1210 cell-transplanted mice, and enhances T cell-mediated immunity and phagocytic activity in vincristine-injected mice.