• International Journal of Stem Cells
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• v.16 no.1
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• pp.93-107
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• 2023

Background and Objectives: Chronic periodontitis can lead to alveolar bone resorption and eventually tooth loss. Stem cells from exfoliated deciduous teeth (SHED) are appropriate bone regeneration seed cells. To track the survival, migration, and differentiation of the transplanted SHED, we used super paramagnetic iron oxide particles (SPIO) Molday ION Rhodamine-B (MIRB) to label and monitor the transplanted cells while repairing periodontal bone defects. Methods and Results: We determined an appropriate dose of MIRB for labeling SHED by examining the growth and osteogenic differentiation of labeled SHED. Finally, SHED was labeled with 25 ㎍ Fe/ml MIRB before being transplanted into rats. Magnetic resonance imaging was used to track SHED survival and migration in vivo due to a low-intensity signal artifact caused by MIRB. HE and immunohistochemical analyses revealed that both MIRB-labeled and unlabeled SHED could promote periodontal bone regeneration. The colocalization of hNUC and MIRB demonstrated that SHED transplanted into rats could survive in vivo. Furthermore, some MIRB-positive cells expressed the osteoblast and osteocyte markers OCN and DMP1, respectively. Enzyme-linked immunosorbent assay revealed that SHED could secrete protein factors, such as IGF-1, OCN, ALP, IL-4, VEGF, and bFGF, which promote bone regeneration. Immunofluorescence staining revealed that the transplanted SHED was surrounded by a large number of host-derived Runx2- and Col II-positive cells that played important roles in the bone healing process. Conclusions: SHED could promote periodontal bone regeneration in rats, and the survival of SHED could be tracked in vivo by labeling them with MIRB. SHED are likely to promote bone healing through both direct differentiation and paracrine mechanisms.

• Journal of Korean Neurosurgical Society
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• v.30 no.4
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• pp.417-424
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• 2001

Objective : Adrenal medullary chromaffin cells are known to release analgesic substances such as opioides and catecholamines. Transplantation of them is a novel method that challenges current approaches in treating chronic pain. The transplantation of xenogeneic chromaffin cells into the central nervous system(CNS) supply antinociception in animals. In this study, we investigated the analgesic effects of rat adrenal medullary chromaffin cells transplanted into the CNS of the mouse. To study the antinociceptive efficacy of transplanted chromaffin cells, the survival of rat adrenal medullary chromaffin cells transplanted into the CNS of mouse was determined. Methods : The adrenal medullary chromaffin cells isolated from rat were transplanted into the striatum of mouse. These cells were confirmed of the release of Met-enkephalin and Leu-enkephalin by HPLC, and immunoblots for tyrosine hydroxylase(TH). Two weeks after transplantation, we performed immunohistochemistry for TH to determine the survival of implanted cells and assessed pain sensitivity at the same time. Results : The isolated rat adrenal medullary chromaffin cells were positive for anti-TH antibody and released Met-enkephalin and Leu-enkephalin more than rat endothelial cells. Transplanted rat chromaffin cells were stained with anti-TH antibody in striatum of mouse after 2 weeks. Pain sensitivity was reduced on the chromaffin cell-transplanted mouse compared to endothelial cell-transplanted mouse by the hot plate test. Conclusion : These results suggest that the rat chromaffin cells were suitably transplanted into the CNS of mouse. This approach could be used as a therapy for reducing of chronic pain induced by cancer or neuronal injury.

• Journal of agriculture & life science
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• v.50 no.4
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• pp.59-71
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• 2016

The occurrence and growth of grass and sedge weed species were investigated in the transplanted rice fields that have different transplanting dates from Apr. 30 to Jun 19 with 10-day interval. The growth and yield of rice plant grown without weed control examined in each plot that has different transplanting dates. Both grass and sedge weeds showed greater plant numbers in the early transplanted plots(Apr. 30 and May 10) than late transplanted plots. Based on the occurring number and dry weight of weeds, the experimental plots were classified into two groups, early group and late group. Weed occurrence and growth were not dramatically different within a group even the occurrence was enhanced as delayed transplanting. Echinochloa spp. that was the most problematic weed showed higher dry weight in early-transplanted field until July, however, the greater dry weight was observed in the late-transplanted plots after August. Sedge weeds including Eleocharis kuroguwai and Scirpus juincoides showed persistently higher value of dry weight in early-transplanted plots than late plots over the experimental period. As the puddling was conducted earlier, emergences of grass and sedge weeds were occurred early and weed growth rate became greater. Therefore, growth and yield of rice that transplanted early decreased more strongly due to the strong suppression by grass and sedge weeds showing the enhanced weed growth rates in early-transplanted plots. Based on the weed growth rate, the adverse effects of grass weeds was maintained for longer period than sedge weeds that showed higher growth rates before heading date of rice plant.

• Journal of Periodontal and Implant Science
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• v.23 no.2
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• pp.261-281
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• 1993

The author transplanted periodontally-diseased teeth which had been treated with citric acid into a clinically healthy extraction sockets and periodontally-affected extraction sockets, and compared with the healing processes within these tissues. Recipient sites were prepared by surgically removing a part of alveolar bone of premolars of adults dogs, placing elastic orthodontic ligatures for 8weeks, thereby inducing periodontal disease. The diseased roots were extracted and transplanted into healthy extraction sockets, and these were designated as control group 1. Diseased roots transplanted into diseased sockets were designated as control group 2. Diseased roots which had been root planed, treated with citric acid and transplanted into healthy sockets were designated as experimental group 1, while identically treated roots which had been transplanted into diseased sockets were designated as experimental group 2. Observations were made at weeks 2, 8 and 12, with following results. 1. At week 2, experimental group 2 showed some inflammatory cell infiltration in the connective tissue above the extraction sockets, while control groups showed less inflammatory or foreign body reactions throughout the experiment. 2. In both control groups, root surface resorption was observed throughout the experiment, while experimental groups showed a little resorption. 3. Control group 1 & 2 showed ankylosis by newly-formed bone ground the resorbed root surfaces, while experimental group 1 & 2 displayed collagen fibers which are not functionally-arranged, with random, loose arrangement or parallel orientation to root surfaces, and newly-formed bone outside of them. 4. In both control groups & experimental groups which had been transplanted into a clinically healthy extraction sockets & periodontally affected extraction sockets groups, histological differences were not significant. 5. Root resorption or ankylosis in control group 1 & 2 had increased quantitatively as experiment progressed. 6. New bone formation developed from the base and lateral wall of extraction sockets. In both control groups & experimental groups, root surfaces lying next to the upper portion of extraction sockets showed little alveolar bone formation and surrounded by connective tissue fiber at weeks 2 & 8, while at weeks 12, they did show alveolar bone formation. 7. At week 12, experimental group 2 showed numerous cells which appeared to be periodontal ligament cells, with functionally arranged connective tissue fibers between the roots and alveolar bone.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.57 no.3
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• pp.233-237
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• 2012

The yield of Unkwangbyeo transplanted on May 10 was 596kg/10a in 2010. The rice yield decreased with delaying transplanting date. The head rice rate was 91.9% when transplanted in June 10, and the rate decreased as the transplanting got earlier. In 2011, the yield increased by 5~8% when transplanted on June 10 because of the increase of Ripened grain ratio and 1,000 grain weight. The yield, when transplanted in the distance of $30{\times}13cm$ with 5 plants, was higher than the other treatments. The yield of Oraebyeo, transplanted on June 10, decreased by 6% compared with transplanting on May 25, while the yield, transplanted in the distance of $30{\times}13cm$ with 5 plants, increased by 5% because of the increase in the number/$m^2$ of panicle and grain. The head rice rates of the two varieties increased by 5~7% and the Toyo palatability appeared to get improved when transplanted on June 10 possibly because of the effect of low ripening temperature by 0.9~$1.6^{\circ}C$ with delaying heading date from 9 to 12 days.

• The Journal of Korean Obstetrics and Gynecology
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• v.19 no.2
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• pp.141-161
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• 2006

Purpose : This study was performed to investigate the effects of Sobokchukeo- Tang(SCT) on the experimentally-induced endometriosis in rats. Materials and Methods : Endometriosis was induced via the surgical autotransplantation technique in rats. A laparotomy was performed and a $4\;{\times}\;4\;mm$ of the right uterine horn was resected incised and sutured to the peritoneum. And the animals divided into control(n=8) and SCT-treated group(n=8). SCT(1,000 mg/head) was administered orally for 15 days after operation. The weights(body, left uterus, and ovaries) and concentrations of cytokines(MCP-1,$TNF-{\alpha}$, $IL-l{\beta}$) were measured. Histopathology, immunohistochemistry for COX-2, and histochemistry for mast cells of the transplanted uterine tissues were performed. Results : - The $volume(mm^3)$ of transplanted uterine tissues of SCT-treated group$(92.88{\pm}41.89)$ was significantly(p<<0.01) decreased than the control group $(404.50{\pm}317.68)$. The concentration(pg/ml) of MCP-1 in ascites of SCT-treated group$(5,256{\pm}1,209)$ was significantly(p<<0.001) decreased than the control group$(8,632{\pm}1,245)$. - The concentration(pg/ml) of $TNF-{\alpha}$ in ascites of SCT-treated group$(521.8{\pm}306.1)$ was significantly(p<<0.01) decreased than the control group$(1,245.2{\pm}362.2)$. - The percentage of COX-2 positive epithelial layer in transplanted uterine tissues of SCT-treated group$(25.0{\pm}7.3)$ was significantly(p<<0.001) decreased than the control group$(50.2{\pm}8.2)$. - The number of mast cells in the stroma of transplanted uterine tissues of SCT-treated group$(16.5{\pm}6.8)$ was significantly(p<<0.05) decreased than the control group$(26.0{\pm}7.7)$. - The number of mast cells in the periphery of transplanted uterine tissues of control group$(71.3{\pm}18.5)$ was significantly(p<<0.01) decreased than the control group$(109.3{\pm}30.2)$. - Proliferation of epithelia, infiltration of inflammatory cells, and microanglogenesis in transplanted uterine tissues of treated group were weakly observed than the control group. Conclusion : From the above results, Sobokchukeo-Tang(SCT) has an inhibitory effect on the development of transplanted uterine tissue in rats and it is related to the decreased concentration of MCP-1 and $TNF-{\alpha}$, and decreased expression of COX-2, and decreased infiltration of mast cells by administration of Sobokchukeo-Tang.

• Phonetics and Speech Sciences
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• v.4 no.3
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• pp.59-73
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• 2012

This study attempts to investigate the contribution of prosody to the foreign accent in Chinese speakers' English production by examining the synthesized speech of crossing native and non-native talkers' prosody and segments. For the stimuli of the foreign accent ratings, we transplanted gender-matched native speakers' prosody onto non-native talkers' segments and vice versa, utilizing the TD-PSOLA algorithm. Eight English native listeners participated in judging foreign accent and comprehensibility of the transplanted stimuli. Results showed that the synthesized stimuli were perceived as stronger foreign accent regardless of speakers' proficiency when English speakers' prosody was crossed with Chinese speakers' segments. This suggests that segments contribute more than prosody to native listeners' evaluation of foreign accent. When transplanted with English speakers' segments, Chinese speakers' prosody showed a difference in duration rather than pitch between high and low proficiency such that stronger foreign accent was detected when low proficient Chinese speakers' duration was crossed with English speakers' segments. This indicated that prosody, more specifically duration, plays a role though the prosodic role is not overall as significant as segments. According to the post acoustic analysis, the temporal features contributing to making the duration parameter prominent as opposed to pitch were found out to be speaking rate, pause duration and pause frequency. Finally, foreign accent and comprehensibility showed no significant correlation such that native listeners had no difficulty listening to highly foreign accented speech.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.19 no.1
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• pp.55-64
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• 2006

Objective : The purpose of this study was to investigate effect of Gungguitakli-San(GTS) on the anti-tumor, immunocytes. Methods : This study estimated the proliferation of L1210 and S-180 cell lines, mouse splenocytes and thymocytes in vitro, and estimated the proliferation of L1210 cell, S-180 cell, thymocytes and splenocytes and body weight in S-180 cells-transplanted mice. The cytotoxicity and proliferation of cells were tested using a colorimetric tetrazoliun assay(M1T assay). Results : The results of this study were obtained as follow ; 1. GTS was significantly increased in the proliferation of thymocytes and splenocytes In vitro. 2. GTS was significantly showed cytotoxicity on the L1210 cell lines and 8-180 cell lines in vitro. 3. GTS was significantly showed cytotoxicity on the L1210 cell lines in vivo. 4. GTS was significantly increased in the weight of mice and decreased weight of sarcoma, in S-180 cells transplanted mice. 5. GTS was significantly increased in the period of survive, in S-180 cells transplanted mice. Conclusions : The author thought that GTS had action of anti-cancer by becoming immunocytes activity and by cytotoxicity of cancer cells.

• Journal of Physiology & Pathology in Korean Medicine
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• v.16 no.2
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• pp.311-315
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• 2002

Cellular death by apoptosis is an active process, depending on gene transcription and protein synthesis. It was reported that nitric oxide can induce apoptosis in several cancer cell-lines. We studied effects of Phytolacca esculentum van Houtt (Phytolaccaceae) Radix water extract (PRE) on the proliferation of transplanted-L1210 cells in mice. When PRE (500 mg/kg) was administered orally once a day for 7 days after transplantation of L1210 cells to mice, DNA fragmentation of transplanted-L1210 cells was induced and mitochondrial transmembrane potential of those cells was reduced. Additionally, DNA fragmentation of L1210 cells was induced by the treatment of PRE in vitro. Also, DNA fragmentation of L1210 cells was enhanced by co-culture with the peritoneal macrophages obtained from PRE-administered mice and was partly inhibited by L-NMMA in vitro. PRE enhanced the production of nitric oxide and tumor necrosis factor-α from peritoneal rnacrophages. These results suggest that PRE induces apoptosis of transplanted-L1210 cells via directive action on L1210 cells and stimulation of nitric oxide and tumor neaosis factor-α from macrophages.

• 한국해양학회지
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• v.15 no.1
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• pp.8-16
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• 1980

The seed of Japaness oyster, Crassostrea gigas(Thunberg), cultivated along the southerncoast of Korea have been transplanted and cultivated in both the Seonyu island and the Eocheong island of the western coast of Korea. The present study was carried out to investigate the growth of transplanted oysters, environmental factors influencing their fatness, and differences of two areas between the Seonyu island and the Eocheong island during the period from April to November, 1978. The results obtained were as follows: 1. Maximum height of the shell was 69.2mm (58.4mm on the average) in the island of Seonyu and 64.8mm(53.2mm) in the island of Eocheong, respectively. 2. The fatness of the oysters measured in the island of Seonyu (5.2g/month) was greater than in the island of Eocheong (2.5g/month) 3. The shell height was increased with high temperature while, the shell fatness with low temperature. 4. The growth rate of the transplanted seed oysters was twice as much as that of native ones in the western coast of Korea. 5. Environmental conditions of Seonyu island were more favorable in cultivating oysters than those of the Eocheong island.