Pain-reducing Effect by Transplants of Isolated Xenogeneic Chromaffin Cells in Mouse

추출된 이종 크롬 친화성 세포의 이식에 의한 마우스에서의 통증 완화 효과

  • Han, Young-Min (Department of Neurosurgery, College of Medicine, Hallym University) ;
  • Lee, Jong-Phil (Department of Neurosurgery, College of Medicine, Hallym University) ;
  • Hwang, Hyung-Sik (Department of Neurosurgery, College of Medicine, Hallym University) ;
  • Song, Joon-Ho (Department of Neurosurgery, College of Medicine, Hallym University) ;
  • Park, Sang-Koo (Institute of Environment & Life Science, Hallym University) ;
  • Park, Suk-Ju (Institute of Environment & Life Science, Hallym University) ;
  • Jin, Jae-Kwang (Institute of Environment & Life Science, Hallym University) ;
  • Choi, Eun-Kyoung (Department of Neurology, Massachusetts General Hospital, Havard Medical School) ;
  • Kim, Yong-Sun (Institute of Environment & Life Science, Hallym University) ;
  • Ahn, Myung-Soo (Department of Neurosurgery, College of Medicine, Hallym University)
  • 한영민 (한림대학교 의과대학 신경외과학교실) ;
  • 이종필 (한림대학교 의과대학 신경외과학교실) ;
  • 황형식 (한림대학교 의과대학 신경외과학교실) ;
  • 송준호 (한림대학교 의과대학 신경외과학교실) ;
  • 박상구 (한림대학교 환경생명과학연구소) ;
  • 박석주 (한림대학교 환경생명과학연구소) ;
  • 진재광 (한림대학교 환경생명과학연구소) ;
  • 최은경 (하버드대학교 의과대학 신경과학교실) ;
  • 김용선 (한림대학교 환경생명과학연구소) ;
  • 안명수 (한림대학교 의과대학 신경외과학교실)
  • Received : 2000.05.29
  • Accepted : 2000.09.01
  • Published : 2001.04.28

Abstract

Objective : Adrenal medullary chromaffin cells are known to release analgesic substances such as opioides and catecholamines. Transplantation of them is a novel method that challenges current approaches in treating chronic pain. The transplantation of xenogeneic chromaffin cells into the central nervous system(CNS) supply antinociception in animals. In this study, we investigated the analgesic effects of rat adrenal medullary chromaffin cells transplanted into the CNS of the mouse. To study the antinociceptive efficacy of transplanted chromaffin cells, the survival of rat adrenal medullary chromaffin cells transplanted into the CNS of mouse was determined. Methods : The adrenal medullary chromaffin cells isolated from rat were transplanted into the striatum of mouse. These cells were confirmed of the release of Met-enkephalin and Leu-enkephalin by HPLC, and immunoblots for tyrosine hydroxylase(TH). Two weeks after transplantation, we performed immunohistochemistry for TH to determine the survival of implanted cells and assessed pain sensitivity at the same time. Results : The isolated rat adrenal medullary chromaffin cells were positive for anti-TH antibody and released Met-enkephalin and Leu-enkephalin more than rat endothelial cells. Transplanted rat chromaffin cells were stained with anti-TH antibody in striatum of mouse after 2 weeks. Pain sensitivity was reduced on the chromaffin cell-transplanted mouse compared to endothelial cell-transplanted mouse by the hot plate test. Conclusion : These results suggest that the rat chromaffin cells were suitably transplanted into the CNS of mouse. This approach could be used as a therapy for reducing of chronic pain induced by cancer or neuronal injury.

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