• Applied Microscopy
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• 제50권
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• pp.11.1-11.3
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• 2020

The human turbinate-derived mesenchymal stem cells (hTMSCs), which were DiI-labeled and transplanted into the subretinal space in degenerating mouse retina, were observed in retinal vertical sections processed for rhodopsin (a marker for rod photoreceptor) by confocal microscope with differential interference contrast (DIC) filters. The images clearly demonstrated that DiI-labeled hTMSCs have rhodopsin-immunoreactive appendages, indicating differentiation of transplanted hTMSC into rod photoreceptor. Conclusively, the finding suggests therapeutic potential of hTMSCs in retinal degeneration.

• 환경생물
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• 제41권1호
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• pp.18-30
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• 2023

우리나라는 산림이 64%에 이르기 때문에 개발사업에 의한 산림훼손이 불가피하다. 이에 대한 방안으로 환경영향평가 제도에서는 훼손되는 수목량의 10%를 재활용 및 이식하도록 하고 있다. 그러나 환경적 요건이 고려되지 않아 이식성공률이 저조하고 가이식장 운영이 잘 되지 않아 수목이 고사하는 등 문제가 지속적으로 발생하고 있다. 이러한 실태를 파악하기 위해서는 현장조사가 필수적이나 시간 및 공간적 한계가 존재한다. 본 연구에서는 원격탐사 기반의 식생지수를 적용하여 개발사업으로 인해 발생하는 이식수목 현황의 시계열적 변화를 탐지하고 원격탐사의 적용성 평가를 목적으로 한다. 이를 위해 위성영상을 분석하여 가이식장 면적을 구축하고 이식 전, 후 식생지수의 시계열 변화를 분석하여 식생 상태를 도출하였다. 연구 결과는 현장조사를 통한 이식수목의 고사율 및 활력도와 위성영상 기반으로 한 가이식 전 후의 식생지수 변화 분석의 결과가 유사한 경향성을 나타내었다. 이에 따라 가이식장에 수목 이식 후에는 가이식장 범위의 녹색 식물의 상대적 분포량과 활동성이 증가하고 시간이 지남에 따라 수목 고사 및 활력도 감소로 인해 낮아지는 것을 규명하였다. 본 연구를 통해 위성영상에 기반한 이식수목 평가 방법을 제시하였으나, 실제 평가에 적용하기 위해서는 보다 정량적인 방법론을 개발할 필요가 있을 것으로 사료된다. 본 연구는 원격탐사 기법인 위성영상과 식생지수를 활용하여 보다 전국에 분포한 이식수목의 변화를 탐지하여, 개발사업으로 인해 시행되는 환경영향평가 제도의 수목 이식이 제대로 수행되고 산림 파괴에 효과적인 저감 대책을 마련하는 데 기여할 수 있을 것으로 기대된다.

• 한국약용작물학회지
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• 제14권3호
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• pp.153-157
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• 2006

작물의 개화는 일장과 저온 뿐만 아니라 식물체의 크기에 의하여도 영향을 받는다. 본 연구는 바위솔의 주년재배에 대한 정보를 확보하고자 유묘를 대 (본엽 18매), 중 (본엽 13매), 소 (본엽 8매)의 3개군으로 분리하여 정식한 후 개화기에 소화의 개화를 억제하기 위하여 night-break를 처리하면서 재배된 바위솔의 생장과 개화에 미치는 영향을 조사하였던 바 그 결과를 요약하면 다음과 같다. 1. 초장, 화서장, 엽과 포엽수 및 경직경은 정식되는 유묘의 크기가 클수록 길고 많거나 굵어지는 경향을 보였다. 2. 엽과 포엽중, 소화중, 경중, 근중, 지상부중, 전체건물중은 정식되는 유묘의 크기가 클수록 많은 것으로 조사되었다. 3. 형성되는 소화수는 정식되는 유묘의 크기가 클수록 많았던 반면, 소화의 개화는 정식되는 유묘의 크기와 관계없이 전혀 일어나지 않았다. 4. 정식 유묘의 크기는 형태, 생장과 소화의 형성에 영향을 미친다고 할 수 있으나 소화의 개화에는 영향이 없기 때문에 바위솔의 주년재배는 정식 유묘의 크기에 영향을 거의 받지 않았다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.317-317
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• 2017

We performed this experiment to select high quality rice adapted to wheat-rice double cropping system. We sowed barley and wheat seed in November 2, 2015. After harvesting barley and wheat, we transplanted high quality rice cultivars: 'Unkwang', 'Hopun', 'Haepum', 'Hyunoum' in June 17 and 24. We used 'sindongjin' rice as a control. As a result, 'Hyunpum' had a highest head rice yield regardless of transplanting date. Head rice yield of 'Hyunpum' was 488.1kg/10a when transplanted in June 17, and 453.6kg/10a when transplanted in June 24. The reason for this highest head rice yield was not percentage of head rice but milled rice yield. Milled rice yield of 'Hyunpum' was 526.5kg/10a when transplanted in June 17, and 490.0kg/10a when transplanted in June 24. Percentage of head rice had little differences between rice cultivars. Among yield components, only number of panicle per $m^2$ had an effect on the differences of yield between rice cultivars. The other yield components didn't have an effect on the differences of yield between rice cultivars. Compared to June 17, only 'Unkwang' had higher head rice percentage and head rice yield in June 24. Head rice percentage of 'Unkwang' changed from 67.3% to 85.0% and head rice yield changed from 324kg/10a to 393.8kg/10a when transplanting date delayed from June 17 to June 24. When transplanting date was delayed, heading date of 'Unkwang' was more delayed than other rice cultivars. By delay of heading date, mean temperature for 40days changed from $25.0^{\circ}C$ to $22.9^{\circ}C$ which improved temperature condition during grain filling stage. This improved head rice percentage and head rice yield of 'Unkwang' transplanted in June 24. If transplanting date is more delayed than June 24, 'Unkwang' could have higher head rice percentage and head rice yield. Therefore, if you transplant rice before June 24 in rice-wheat double cropping system, 'Hyunpum' is recommended as adequate rice cultivar. But if you transplant rice after June 24, further research is needed to find out adequate rice cultivar.

• ALGAE
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• 제20권4호
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• pp.379-388
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• 2005

Seagrass bed is an important component in coastal and estuarine ecosystems, providing food and shelter to a wide variety of fauna. Recently, seagrass coverage has declined significantly due to anthropogenic influences such as reclamation, dredging, and eutrophication and consequently, necessity of seagrass habitat restoration is rising. Transplantation experiments with Zostera marina using TERFS, staple method, and shell method have been conducted at Dadae Bay, Kosung Bay and Jindong Bay on the south coast of Korea to select an optimal transplanting method for restoration of Z. marina habitat. Three experimental sites located at the vicinity of natural Z. marina beds with an average water depth of about 4m. Z. marina plants, which were collected from donor bed in Koje Bay were also transplanted at 7 different time from October 2003 to July 2004 to find appropriate transplanting time. Density of Z. marina was monitored monthly at both transplanted areas and natural beds. Transplantation using the staple method showed the highest survival rate of transplant. Shell method was also an effective transplanting method at muddy areas in Kosung Bay and Jindong Bay, but not suitable at sandy areas in Dadae Bay. These results suggest that sediment composition of transplanting areas should be considered for the selection of the optimal transplanting method. Z. marina transplanted during fall usually showed the highest survival rate, while most Z. marina plants transplanted in summer died due to high lethal temperature during this period.

• 유기물자원화
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• 제8권2호
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• pp.85-92
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• 2000

자연간석지 토양을 인공간석지에 인공간석지 토양을 자연간석지에 이식하고, 이식토양의 물리화학적 및 생물학적 특성의 변화를 추적하였다. 또한 실트함량과 유기물함량이 미생물현존량에 미치는 영향에 관해서 실내실험을 통하여 검토하였다. 자연간석지 토양을 인공간석지에 인공간석지 토양을 자연간석지에 이식한 결과, 실트함량, 유기물함량, 미생물현존량 및 토양중의 산화환원전위층은 시간의 경과와 함께 이식한 간석지의 토양구조와 동일하게 변이해 가는 것을 알았다. 또한 이식 토양중의 실트함량, 미생물현존량 및 유기물함량과는 높은 상관관계를 나타내었다. 실트함량 및 유기물함량의 증감과 함께 미생물현존량이 비례해서 증감하는 것을 알았다. 또한 실내실험 결과에서도 실트함량이나 유기물함량의 증가와 함께 미생물현존량이 증가하였다. 또한 그 원인은 실트함량이 증가함에 따른 결과라는 것을 알 수 있었다.

• Journal of Korean Neurosurgical Society
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• 제42권2호
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• pp.118-124
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• 2007

Objective : Adipose tissue is derived from the embryonic mesoderm and contains a heterogenous stromal cell population. Authors have tried to verify the characteristics of stem cell of adipose derived stromal cells (ADSCs) and to investigate immunohistochemical findings after transplantation of ADSC into rat brain to evaluate survival, migration and differentiation of transplanted stromal cells. Methods : First ADSCs were isolated from human adipose tissue and induced adipose, osseous and neuronal differentiation under appropriate culture condition in vitro and examined phenotypes profile of human ADSCs in undifferentiated states using flow cytometry and immunohistochemical study. Human ADSCs were transplanted into the healthy rat brain to investigate survival, migration and differentiation after 4 weeks. Results : From human adipose tissue, adipose stem cells were harvested and subcultured for several times. The cultured ADSCs were differentiated into adipocytes, osteoctye and neuron-like cell under conditioned media. Flow cytometric analysis of undifferentiated ADSCs revealed that ADSCs were positive for CD29, CD44 and negative for CD34, CD45, CD117 and HLA-DR. Transplanted human ADSCs were found mainly in cortex adjacent to injection site and migrated from injection site at a distance of at least 1 mm along the cortex and corpus callosum. A few transplanted cells have differentiated into neuron and astrocyte. Conclusion : ADSCs were differentiated into multilineage cell lines through transdifferentiation. ADSCs were survived and migrated in xenograft without immunosuppression. Based on this data, ADSCs may be potential source of stem cells for many human disease including neurologic disorder.

• Journal of Korean Neurosurgical Society
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• 제38권2호
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• pp.121-125
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• 2005

Objective : Many recent reports have shown that the mature mammalian brain harbors multipotent stem cells, rendering the brain capable of generating new neurons and glia throughout life. Harvested stem cells from an adult rat are transplanted in order to evaluate the cell survival and differentiation. Methods : Using a percoll gradient with a high speed centrifugation method, we isolate neural stem/progenitor cells were isolated from the subventricular zone[SVZ] of a syngeneic adult Fisher 344 rats brain. For 14days expansion, the cultured cells comprised of a heterogeneous population with the majority of cells expressing nestin and/or GFAP. After expanding the SVZ cells in the presence of basic fibroblast growth factor-2, and transplanting then into the hippocampus of normal rats, the survival and differentiation of those cells were examined. For transplantation, the cultured cells were labeled with BrdU two days prior to use. In order to test their survival, the cells were transplanted into the dorsal hippocampus of normal adult Fisher 344 rats. Results : The preliminary data showed that at 7days after transplantation, BrdU+ transplanted cells were observed around the injection deposition sites. Immuno-fluorescent microscopy revealed that the cells co-expressed BrdU+ and neuronal marker ${\beta}$-tubulin III. Conclusion : The data demonstrate that the in vitro expanded SVZ cells can survive in a heterotypic environment and develop a neuronal phenotype in the neurogenic region. However more research will be needed to examine the longer survival time points and quantifying the differentiation in the transplanted cells in an injured brain environment.

• Clinical and Experimental Reproductive Medicine
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• 제26권2호
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• pp.219-223
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• 1999

Ovarian development of the vitrified neonatal ovaries after orthotopical transplantation into the ovariectomized adult recipient mouse were observed. Ovaries were collected from the neonatal females on day of birth and grouped for fresh, vitrification for 1-minute, and 3-minute. Vitrified and thawed neonatal ovaries were orthotopically transplanted into ovarian bursa of the adult mice from which endogenous ovaries have removed just prior to the transplantation (1 minute: n=25; 3 minutes n=23). Fresh ovarian tissue transplanted (n=25) mice were included as control groups. Returning of the estrus cycles and the survival and development of the transplanted ovaries were evaluated. Intact ovaries from neonatal, and four weeks old mice were used for comparison of the ovarian development as in vivo-developed control. From 2 weeks after transplantation, 64%, 36%, and 75% of the transplanted mice showed return of the estrus cycles in fresh, 1-minute, and 3-minute groups, respectively. Four weeks after transplantation, all mice were sacrificed and ovarian tissues were recovered for histological analysis. 57.1%, 33.3%, and 64.7% mice in fresh, 1-minute, and 3-minute groups, respectively, had survived ovaries with follicles at various stages of growth from primordial to preovulatory follicles. Corpus lutea were also observed. Results of the present study suggest that 1) normal folliculogenesis has initiated in vivo after vitrification, and 2) the vitrification may be used as a preservation method for ovarian tissues for establishment of ovarian tissue bank.

• 대한한방부인과학회지
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• 제19권1호
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• pp.125-138
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• 2006

Purpose : This study was to investigate the effects of Dohongsamul-tang extract on the rat with an surgically induced endometriosis. Methods : Four weeks after the operation for inducing the endometriosis to a matured female rat, the normal hyperplasia of the transplanted endometrium was identified in anatomico-histological aspects. By dividing each of the control group and Dohongsamul-tang administered experimental group into 8 rats, Dohongsamul-tang concentrates were administered orally to the experimental group with 1g/1ml/200g everyday for 40 days and then images were taken from the macroscopic tissues which were transplanted to the mesenterium, and the concentrations of progesterone, estradiol, tumor necrosis $factor-{\alpha}(TNF-{\alpha})$, and interleukin(IL)(-2, -4, -6, and -10) in the serum were measured. Results : Transplanted endometrium tissues showed the histological findings in accordance with the normal endometrium tissues and from the macroscopic findings the size of transplanted endometrium tissues showed a definite decrease. Compared to the control group, the experimental group showed significant decreases in the values of $TNF-{\alpha}$,IL-2, and IL-4 and significant increases in the values of IL-10. However, there were no significant differences in progesterone, estradiol and IL-6. Conclusion : From the above results, Dohongsamul-tang showed the strengthening of immunological function and anti-inflammatory effect. Therefore it is considered that Dohongsamul-tang will be very effective on the treatment of inflammation developed endometriosis, especially to treat the endometriosis without affecting the ovary functions.