Applied Microscopy

  • Volume 50
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  • Pages.11.1-11.3
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  • 2020
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  • 2287-5123(pISSN)
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  • 2287-4445(eISSN)
Korean Society of Microscopy (한국현미경학회)
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Light microscopic evidence of in vivo differentiation from the transplanted inferior turbinate-derived stem cell into the rod photoreceptor in degenerating retina of the mouse

  • Yong Soo Park (Department of Anatomy, College of Medicine, The Catholic University of Korea) ;
  • Yeonji Kim (Department of Anatomy, College of Medicine, The Catholic University of Korea) ;
  • Sung Won Kim (Department of Otolaryngology-Head and Neck Surgery, Seoul St Mary's Hospital, College of Medicine, The Catholic University of Korea) ;
  • In-Beom Kim (Department of Anatomy, College of Medicine, The Catholic University of Korea)
  • Received : 2020.04.19
  • Accepted : 2020.05.20
  • Published : 2020.12.31
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Abstract

The human turbinate-derived mesenchymal stem cells (hTMSCs), which were DiI-labeled and transplanted into the subretinal space in degenerating mouse retina, were observed in retinal vertical sections processed for rhodopsin (a marker for rod photoreceptor) by confocal microscope with differential interference contrast (DIC) filters. The images clearly demonstrated that DiI-labeled hTMSCs have rhodopsin-immunoreactive appendages, indicating differentiation of transplanted hTMSC into rod photoreceptor. Conclusively, the finding suggests therapeutic potential of hTMSCs in retinal degeneration.

Keywords

Acknowledgement

This work was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI15C3076) and a grant of the Dios Pharma R&D 2019 Project.

References

  1. H.M. Blau, G.Q. Daley, Stem cells in the treatment of disease. N. Engl. J. Med. 380, 1748-1760 (2019)
  2. S.H. Hwang, S.Y. Kim, S.H. Park, M.Y. Choi, H.W. Kang, Y.J. Seol, J.H. Park, D.W. Cho, O.K. Hong, J.G. Rha, S.W. Kim, Human inferior turbinate: An alternative tissue source of multipotent mesenchymal stromal cells. Otolaryngol. Head Neck Surg. 147, 568-574 (2012)
  3. S.H. Hwang, S.H. Park, J. Choi, D.C. Lee, J.H. Oh, S.W. Kim, J.B. Kim, Characteristics of mesenchymal stem cells originating from the bilateral inferior turbinate in humans with nasal septal deviation. PLoS One 9(6), e100219 (2014)
  4. H. Lim, S.H. Park, S.W. Kim, K.O. Cho, Therapeutic potential of human turbinatedderived mesenchymal stem cells in experimental acute ischemic stroke. Int. Neurourol. J. 22(Suppl 3), S131-S138 (2018)
  5. R.A. Pearson, Advances in repairing the degenerate retina by rod photoreceptor transplantation. Biotechnol. Adv. 32, 485-491 (2014)
  6. A. Rattner, J. Nathans, Macular degeneration: Recent advances and therapeutic opportunities. Nat. Rev. Neurosci. 7, 860-872 (2006)