• Journal of Veterinary Clinics
• /
• v.15 no.2
• /
• pp.442-449
• /
• 1998

Freeze-dried cortical bones of the goat were transplanted to the experimental fibular defect of 10 dogs for valuating the possibility of xenogeneic bone implantation and the specificity of BM(Bone Morphogenetic Protein). The . freeze-dried cortical bone eliminated antigens and defatted with chloroform and methanol were freeze-dried at $-80{\circ}C$ for preservation of BMP and then sterilized with 50 gas and storaged in room temperature. Ten freeze-dried cortical implants of the goat were transplanted in experimentally defected regions of bilateral fibula of 5 dogs in clinically normal. The transplanted region had been radiographed for observing state of bone union and BALPOone Alkaline Phosphatase) in the serum of the host was measured for valuating activity of oteoblast per 2 week-interval after transplant procedures. New bone formation had been observed early in one of ten regions around implants about the same time as autoimplant regions. It was incorporated with its host bone during 4-12 weeks after transplantation. In another 2 cases of 2 dogs, new bone formation and absorption of implant had been observed from 4 weeks but they were not incorporated completely until 20 weeks. The rest of the freeze-dried bone implants, 7 cases of 4 dogs had not been observed new bone formation nor absorption of implants. The freeze-drying method for implants means to not influence bone incorporation. Although less of union percentages the union form of this experiment were similar to alloimplantation and it may mean to block immunity reaction that disturbs the bone induction by BMP. It demonsknted that the possibility of the xenogenous bone implantation is recognized by reason of the low specificity of BMP between goat and dog.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.25 no.3
• /
• pp.459-465
• /
• 2011

The nuclear factor of activated T cells (NFAT) protein induces transcriptions of cytokine genes including IL-2 for T-cell activation. Normally activation of NFAT is important to induce immune responses but excessive NFAT activation provokes immunopathological reactions such as autoimmunity, transplant rejection, and inflammation. Thus, for the treatment of autoimmune diseases drugs repressing the activation of NFAT have been searched. In this study, immnunosuppressive effects of Rosa chinensis Jacq. extracts identified as a potent NFAT inhibitor from a natural product library were examined. NFAT reporter assay, MTS assay, real time PCR, IL-2 ELISA, MLR, and FACS (Fluorescent Activated Cell Sorting) were used to measure inhibitory immunocyte activities of Rosa chinensis Jacq. The variety of natural products have been screened and some were found to show inhibitory activities against the NFAT transcription factor. Among them, extract of Rosa chinensis Jacq. showed an strong inhibitory effect on the activation of NFAT without affecting cell viability. Levels of IL-2 transcripts as well as IL-2 protein were decreased with treatment of Rosa chinensis Jacq. extract. In addition, immunosuppressive activity of Rosa chinensis Jacq. extract was exhibited in the mixed leukocytes reaction. The increasement of CD4+CD25+ (Treg) immunocyte was also detected in the analysis using FACS after applying Rosa chinensis Jacq. extract. Immunosuppressive effects of the Rosa chinensis Jacq. extracts were clearly demonstrated in the present study. In addition, Rosa chinensis Jacq. extract also positively affected regulatory T cell induction. Further investigations in particular on purification of single substance responsible for the immunosuppressive effects from the extract and analysis on possible actions of the extract in interfering cell signaling and cytokine production will be needed.

• The Journal of Korean Society of Virology
• /
• v.30 no.2
• /
• pp.125-130
• /
• 2000

Infection with human cytomegalovirus (HCMV) is of considerable clinical relevance after placental transmission and in immunosuppressed patients such as transplant recipients or patients with AIDS. The rapid detection method of HCMV has been required to overcome the time-consuming methods such as classical plaque assay or other immunological methods. This study was performed to establish the in situ ELISA, in which human lung fibroblasts infected with HCMV were fixed and used directly as antigen in 96 well culture plate. Expressed HCMV antigens were detected with HCMV-specific monoclonal antibodies. This method could detect HCMV dose-dependently upto $3{\times}10^2\;pfu/ml$. Antiviral activity of ganciclovir could be assayed within the known range of effective dose. This result showed that HCMV could be quantitated by in situ ELISA. The chemical, which was selected on the basis of component analysis in natural product, was tested to have the anti-HCMV activity by in situ ELISA, and three among five samples were found to have anti-HCMV activity with the dose-dependent manner. Conclusively in situ ELISA could be useful method for quantitation of HCMV and screening antiviral activity of samples to HCMV.

• Journal of Bio-Environment Control
• /
• v.10 no.1
• /
• pp.55-60
• /
• 2001

To investigate the effect of red light (R) and far-red light(FR) on controlling the growth of tomato transplants, height, length of 1st internode, and stem diameter of plant were measured every 12 days during 24 days of light treatment. At the end of experiment, fresh and dry weights of roots and shoots were measured. Generally, it was shown that the height of plant was suppressed by the treatment of FR. However, the effect of light-treatment time (10 or 20 min) on plant height was not significant. Stem diameter of the plant treated with R was greater than that of the plant treated with FR or the control. Dry weight ratio of shoot to root of the plant treated with R was smaller that of the plant treated with FR. R was more effective than the control, which was more effective than FR, in making the transplant compact.

• Journal of Korean Dental Hygiene Science
• /
• v.1 no.2
• /
• pp.61-71
• /
• 2018

This study set out to examine the characteristics and consciousness of biomedical ethics among dental hygiene and nursing students and provide basic data for the education of biomedical ethics in the department of dental hygiene. The subjects include 158 and 128 students in the dental hygiene and nursing departments, respectively, at a university in Gyeongnam. Collected data was analyzed with the SPSS 12.0 program. 1. The analysis results show that the dental hygiene and nursing students scored mean 2.72 and 2.65 points in biomedical ethics consciousness, respectively, with statistical significance(p<.000). 2. In the subareas of biomedical ethics, the two groups showed the highest and lowest level of consciousness in organ transplant and euthanasia, respectively. 3. The two groups had differences in characteristics related to biomedical ethics by the major such as ethical values(p<.05), experiences with biomedical ethics education(p<.01), time of biomedical ethics education(p<.001), sources of information and knowledge about biomedical ethics(p<.01), experiences with conflicts in biomedical ethics(p<.000), and willingness to participate in education(p<.05). 4. The nursing students recorded higher biomedical ethics consciousness than the dental hygiene students with no statistically significant differences between them. Biomedical ethics consciousness had positive correlations with ethical values(r=.122) and experiences with biomedical ethics education(r=.356). Based on these findings, the study proposed the development of educational content for biomedical ethics consciousness and research on its effectiveness to help dental hygiene students form desirable biomedical ethics consciousness.

• Journal of The Korea Institute of Healthcare Architecture
• /
• v.30 no.2
• /
• pp.15-22
• /
• 2024

Purpose: In general, cross-infection caused by bacteria occurs more in hospitals than in local communities. In most cases, infectious diseases spread through contact transmission (direct contact, indirect contact). This study tries to examine which places are most likely to detect infections bacteria and what materials should be used to effectively suppress the spread of infectious bacteria. Methods: Domestic and international literature have been reviewed to determine which bacteria are common and spread in which places. At the same time, antibacterial experiments for several finishing materials are performed to determine the survival period of bacteria for each material. The experiment is conducted mainly on antibiotic-resistant bacteria (MRSA, CRE, etc.) that have a high mortality rate and are very contagious. Results: MRSA has a high incidence in many hospital departments with surgery or immunocompromised patients, such as the elderly, organ transplant patients, and hemodialysis patients. There are experimental results that MRSA dies early in ceramics or silk wallpaper. CRE has a high incidence in hospital departments where there are many patients who are prone to bacteria entering the body directly, such as ventilator patients or critically ill patients with surgical wounds. There are experimental results that CRE dies early in silk wallpaper. In addition, bacteria die on the surface for a variety of reasons. Most MRSA and CRE develop in patients with impaired immunity or surgery, and rapidly die in copper or materials with antibacterial properties. Implications: If finishing materials surface with the ability to kill specific bacteria is used in the place where a large number of specific bacteria are detected, the spread of infectious diseases can be effectively controlled.

• Annals of Hepato-Biliary-Pancreatic Surgery
• /
• v.27 no.1
• /
• pp.6-19
• /
• 2023

A systematic review was conducted in compliance with PRISMA statement standards to identify all studies reporting outcomes of laparoscopic resection of benign or malignant lesions located in caudate lobe of liver. Pooled outcome data were calculated using random-effects models. A total of 196 patients from 12 studies were included. Mean operative time, volume of intraoperative blood loss, and length of hospital stay were 225 minutes (95% confidence interval [CI], 181-269 minutes), 134 mL (95% CI, 85-184 mL), and 7 days (95% CI, 5-9 days), respectively. The pooled risk of need for intraoperative transfusion was 2% (95% CI, 0%-5%). It was 3% (95% CI, 1%-6%) for conversion to open surgery, 6% (95% CI, 0%-19%) for need for intra-abdominal drain, 1% (95% CI, 0%-3%) for postoperative mortality, 2% (95% CI, 0%-4%) for biliary leakage, 2% (95% CI, 0%-4%) for intra-abdominal abscess, 1% (95% CI, 0%-4%) for biliary stenosis, 1% (95% CI, 0%-3%) for postoperative bleeding, 1% (95% CI, 0%-4%) for pancreatic fistula, 2% (95% CI, 1%-5%) for pulmonary complications, 1% (95% CI, 0%-4%) for paralytic ileus, and 1% (95% CI, 0%-4%) for need for reoperation. Although the available evidence is limited, the findings of the current study might be utilized for hypothesis synthesis in future studies. They can be used to inform surgeons and patients about estimated risks of perioperative complications until a higher level of evidence is available.

• Clinical and Experimental Pediatrics
• /
• v.51 no.1
• /
• pp.67-72
• /
• 2008

Purpose : In this study, we retrospectively analyzed the clinical outcomes of patients who underwent allogeneic hematopoietic stem cell transplantation (HSCT) grafted from HLA-matched parents. Methods : Seven children with acute leukemia (4 acute lymphoblastic leukemia, 3 acute myeloid leukemia) in first complete remission received allogeneic HSCT from their respective parents at the St. Marys Hospital between April, 1999 and October, 2005. The median age of patients at transplantation was 5 years (range, 1-11 years; 2 male, 5 female) and the median age of donors was 35 years (range, 30-41 years; 5 male, 2 female). We investigated the clinical outcomes such as engraftment, acute and chronic graft-versus-host disease (GVHD), transplant-related morbidity and mortality, relapse and survival. Results : Median time from transplantation to last follow-up was 69.5 months (range, 18.8-96.5 months). All patients were successfully engrafted, with a median time of 11 days (range, 10-16 days) and 26 days (range, 13-39 days) for neutrophil and platelet recovery, respectively. Grade II acute GVHD occurred in 3, and grade III acute GVHD in 1 of 7 recipients. Extensive chronic GVHD developed in 2, and limited chronic GVHD in 1 of 7 recipients. Death from transplant-related complications occurred in 1, and relapse occurred in 1 of 7 recipients. Estimated 5-year overall survival was $83{\pm}15%$. Conclusion : The clinical outcomes of recipients who underwent HSCT from HLA-matched parents were comparable to those of patients who received HSCT grafted from HLA-matched sibling donors in childhood leukemia. HLA typing of parents, as well as siblings will increase the likelihood of finding an HLA-matched family donor for patients who need HSCT.

• Horticultural Science & Technology
• /
• v.28 no.5
• /
• pp.768-776
• /
• 2010

This study was conducted to predict number and fresh weight of leaves, and total leaf area of a single-stemmed rose 'Vital' based on the accumulated thermal units, and to develop a model of shoot development for the prediction of the time when the flowering shoot reaches a phenological stage in a plant factory system. The base temperature ($T_b$), optimum temperature ($T_{opt}$), and maximum temperature ($T_{max}$) were estimated by regressing the rate of shoot development against the temperature gradient. The rate of shoot development ($R$, $d^{-1}$) for the phase from cutting to bud break (CT-BB) was best described by a linear model $R_b$ ($d^{-1}$) = -0.0089 + $0.0016{\cdot}temp$. The rate of shoot development for the phase from bud break to harvest (BB-HV) was fitted to the parabolic model $R_h$ ($d^{-1}$) = $-0.0001{\cdot}temp^2$ + $0.0054{\cdot}temp$ - 0.0484. The $T_b$, $T_{opt}$, and $T_{max}$ values were 5.56, 27.0, and $42.7^{\circ}C$, respectively. The $T_b$ value was used in the thermal unit computations for the shoot development. Number of leaves, leaf area (LA), and leaf fresh weight showed sigmoidal curves regardless of the cut time. The shoot development and leaf area model was described as a sigmoidal function using thermal units. Leaf area was described as LA = 578.7 $[1+(thermal units/956.1)^{-8.54}]^{-1}$. Estimated and observed shoot length and leaf fresh weight showed a reasonably good fit with 1.060 ($R^2=0.976^{***}$) and 1.043 ($R^2=0.955^{***}$), respectively. The average thermal units required from cutting to transplant and from transplant to harvest stages were $426{\pm}42^{\circ}C{\cdot}d$ and $783{\pm}24^{\circ}C{\cdot}d$, respectively.

• Korean Journal of Plant Resources
• /
• v.9 no.2
• /
• pp.157-163
• /
• 1996

This study was carried out to control the decrease of yield and the deterioration of quality of Angelica gigas roots by bolting. This phenomenon resulted from lignificartion and dwarfishness of roots because of bolting formed for inflorescence and fruition. Seedlings were grown for 60,90 and 120 days in nursery of the green house, and transplanted in main field to compare with traditional seedling that grew for 1 year. Growth of the petiole length compare with length and leaf width of aerial parts in nursery showed a remarkably progress, and root characters as root length, root diameter and root weight grew more vigorously in the order of nursery periods. Bolting was not entirely formed in plots transplanted the seedlings which grown for short-time; 60, 90 and 120days, but showed the high bolting rate, 64.3%,in traditional seedling plots. Yield was higher in plots that transplanted short-time growing seedlings than that transplanted traditional seedlings, and among 3 kinds of nursery periods yields were high in the order of nursery periods. For the increase of yield and decrease of bolting rate, it was needed to transplant small seedlings of 3-4g/plant which grew in nursery for 90-120 days.