Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Study on Immunosuppressive Effects of Rosa Chinensis Jacq. Extract

월계화 추출물의 면역억제 효능 연구

  • Kim, Kyoung-Shin (Department of Physiology, Colleage of Oriental Medicine, Daejeon University) ;
  • Park, Jae-Won (Department of Physiology, Colleage of Oriental Medicine, Daejeon University) ;
  • Chae, Suhn-Kee (Department of Life Science and Technology, Paichai Univetsity) ;
  • Kang, Jung-Soo (Department of Physiology, Colleage of Oriental Medicine, Daejeon University) ;
  • Kim, Byoung-Soo (Department of Physiology, Colleage of Oriental Medicine, Daejeon University)
  • 김경신 (대전대학교 한의과대학 생리학교실) ;
  • 박재원 (대전대학교 한의과대학 생리학교실) ;
  • 채순기 (배재대학교 생명공학과) ;
  • 강정수 (대전대학교 한의과대학 생리학교실) ;
  • 김병수 (대전대학교 한의과대학 생리학교실)
  • Received : 2011.05.25
  • Accepted : 2011.06.15
  • Published : 2011.06.25
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Abstract

The nuclear factor of activated T cells (NFAT) protein induces transcriptions of cytokine genes including IL-2 for T-cell activation. Normally activation of NFAT is important to induce immune responses but excessive NFAT activation provokes immunopathological reactions such as autoimmunity, transplant rejection, and inflammation. Thus, for the treatment of autoimmune diseases drugs repressing the activation of NFAT have been searched. In this study, immnunosuppressive effects of Rosa chinensis Jacq. extracts identified as a potent NFAT inhibitor from a natural product library were examined. NFAT reporter assay, MTS assay, real time PCR, IL-2 ELISA, MLR, and FACS (Fluorescent Activated Cell Sorting) were used to measure inhibitory immunocyte activities of Rosa chinensis Jacq. The variety of natural products have been screened and some were found to show inhibitory activities against the NFAT transcription factor. Among them, extract of Rosa chinensis Jacq. showed an strong inhibitory effect on the activation of NFAT without affecting cell viability. Levels of IL-2 transcripts as well as IL-2 protein were decreased with treatment of Rosa chinensis Jacq. extract. In addition, immunosuppressive activity of Rosa chinensis Jacq. extract was exhibited in the mixed leukocytes reaction. The increasement of CD4+CD25+ (Treg) immunocyte was also detected in the analysis using FACS after applying Rosa chinensis Jacq. extract. Immunosuppressive effects of the Rosa chinensis Jacq. extracts were clearly demonstrated in the present study. In addition, Rosa chinensis Jacq. extract also positively affected regulatory T cell induction. Further investigations in particular on purification of single substance responsible for the immunosuppressive effects from the extract and analysis on possible actions of the extract in interfering cell signaling and cytokine production will be needed.

Keywords

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