The Journal of Korean Society of Virology (대한바이러스학회지)

The Korean Society of Virology (대한바이러스학회)
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Establishment of Measurement of Human Cytomegalovirus with in situ ELISA

in situ ELISA를 이용한 사람세포거대바이러스의 측정법 개발

  • Hwang, Eung-Soo (Department of Microbiology, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center) ;
  • Kim, Jin-Hee (Department of Microbiology, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center) ;
  • Park, Chung-Gyu (Department of Microbiology, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center) ;
  • Cha, Chang-Yong (Department of Microbiology, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center)
  • 황응수 (서울대학교 의과대학 미생물학교실 및 서울대학교 의학연구원 감염병연구소) ;
  • 김진희 (서울대학교 의과대학 미생물학교실 및 서울대학교 의학연구원 감염병연구소) ;
  • 박정규 (서울대학교 의과대학 미생물학교실 및 서울대학교 의학연구원 감염병연구소) ;
  • 차창룡 (서울대학교 의과대학 미생물학교실 및 서울대학교 의학연구원 감염병연구소)
  • Published : 2000.06.30
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Abstract

Infection with human cytomegalovirus (HCMV) is of considerable clinical relevance after placental transmission and in immunosuppressed patients such as transplant recipients or patients with AIDS. The rapid detection method of HCMV has been required to overcome the time-consuming methods such as classical plaque assay or other immunological methods. This study was performed to establish the in situ ELISA, in which human lung fibroblasts infected with HCMV were fixed and used directly as antigen in 96 well culture plate. Expressed HCMV antigens were detected with HCMV-specific monoclonal antibodies. This method could detect HCMV dose-dependently upto $3{\times}10^2\;pfu/ml$. Antiviral activity of ganciclovir could be assayed within the known range of effective dose. This result showed that HCMV could be quantitated by in situ ELISA. The chemical, which was selected on the basis of component analysis in natural product, was tested to have the anti-HCMV activity by in situ ELISA, and three among five samples were found to have anti-HCMV activity with the dose-dependent manner. Conclusively in situ ELISA could be useful method for quantitation of HCMV and screening antiviral activity of samples to HCMV.

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