• Korean Journal of Adult Nursing
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• v.24 no.6
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• pp.580-587
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• 2012

Purpose: To develop and test the validity and reliability of the Korean version of outcome expectations-2 for exercise. Methods: The Korean version of outcome expectations for exercise-2 was developed through forward-backward translation techniques. Content, criterion, and construct validity using confirmatory factor analysis and an internal consistency reliability were conducted. Survey data were collected from 200 older adults living in a community. Results: The Korean version of outcome expectations for exercise-2 had factor loadings of the 13 items ranged from .20 to .76, and was validated by confirmatory factor analysis (CFI=.829, NFI=.754, RMSEA=.086). Also there was a reliable internal consistency with a Cronbach's ${\alpha}$ for the positive domain of outcome expectations for exercise scale-2 of .73. Negative domain, however, reported slightly low Cronbach's ${\alpha}$ of .63. Conclusion: The findings of this study demonstrated that the Korean version of outcome expectations for exercise-2 had satisfactory validity to measure expectations regarding exercise among older adults in Korea. Negative domain, however, should be retested to verify reliability for the further study.

• Korean Journal of Veterinary Research
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• v.52 no.2
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• pp.99-104
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• 2012

The present study was performed to evaluate the relationship between the neurotoxicity of acrylamide and the differential gene expression pattern in mice. Both locomotor test and rota-rod test showed that the group treated with higher than 30 mg/kg/day of acrylamide caused impaired motor activity in mice. Based on cDNA microarray analysis of mouse brain, myelin basic protein gene, kinesin family member 5B gene, and fibroblast growth factor (FGF) 1 and its receptor genes were down-regulated by acrylamide. The genes are known to be essential for neurofilament synthesis, axonal transport, and neuroprotection, respectively. Interestingly, both FGF 1 and its receptor genes were down-regulated. Genes involved in nucleic acid binding such as AU RNA binding protein/enoyl-coA hydratase, translation initiation factor (TIF) 2 alpha kinase 4, activating transcription factor 2, and U2AF 1 related sequence 1 genes were down-regulated. More interesting finding was that genes of both catalytic and regulatory subunit of protein phosphatases which are important for signal transduction pathways were down-regulated. Here, we propose that acrylamide induces neurotoxicity by regulation of genes associated with neurofilament synthesis, axonal transport, neuro-protection, and signal transduction pathways.

• Women's Health Nursing
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• v.22 no.3
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• pp.151-161
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• 2016

Purpose: The purpose of this study was to develop and test the validity and reliability of the Korean version of the Contraceptive Self-efficacy (K-CSE) scale. Methods: The K-CSE scale was developed through forward-backward translation methods. Construct and criterion validity and internal consistency reliability were calculated using SPSS/WIN 21.0. Data were collected from 257 women students from two universities in G city, South Korea. Results: The factor structure of the K-CSE scale showed the cumulative variance as 62.9% in the factor analysis. Factor loading of 13 items on four subscales ranged from .47 to .88. Factors were named as 'initiative of contraceptive use', 'sexual assertiveness', 'refusal of sexual intercourse', and 'autonomic sexual behavior'. Criterion validity compared to the Sexual Autonomy Measurement (SAM) showed significant correlation. Cronbach's ${\alpha}$ for the reliability of each subscale were .50~.82 and .76 for the total scale. Conclusion: The findings of this study demonstrate that the K-CSE scale is a satisfactory valid and reliable scale. Therefore, the CSE scale can be useful in measuring contraceptive self-efficacy among women university students in Korea.

• Journal of Korean Academy of Nursing
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• v.51 no.5
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• pp.525-536
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• 2021

Purpose: The aim of this study was to assess the Turkish adaptation of the COVID Stress Scale (CSS) on the basis of determining the stress caused by the coronavirus disease (COVID-19) pandemic, and to test its validity and reliability. Methods: The English CSS was translated into Turkish using forward and backward translation. Data were collected online from 360 participants. Construct validity was evaluated using confirmatory factor analysis, exploratory factor analysis, and content validity. Pearson product-moment correlation, Cronbach's alpha reliability coefficient, and test-retest methods were used to evaluate reliability. Results: The Turkish version of the CSS has 36 items consistent with the original scale and has five factors: COVID danger and contamination, socioeconomic consequences of COVID, COVID xenophobia, traumatic stress due to COVID, and compulsive checking for COVID. The construct validity of the Turkish version of the CSS was verified by the adjusted goodness of fit index > .85, and comparative fit index > .95. The content validity index of each item was 91%. The corrected item-total correlations of the scale ranged from .51 to .89. Internal consistency was reliable, with a Cronbach's α of .93. Conclusion: The Turkish version of the CSS is valid and reliable. It can be used as a measurement tool for the assessment of COVID-related stress.

• Journal of Life Science
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• v.7 no.4
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• pp.392-401
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• 1997

The poliovirus is a small, and non-enveloped virus. The RNA genome of poliovirus is continuous, linear, and has a single open reading frame. This polyprotein precursor is cleaved proteolytically to yield mature products. Most of the cleavages occur by viral protease. The mature proteins derived from the P1 polyprotein precursor are the structural components of the viral capsid. The initial cleavage by 2A protease is indirectly involved in the cleavage of a cellular protein p220, a subunit of the eukaryotic translation initiation factor 4F. This cleavage leads to the shut-off of cap-dependent host cell translation, and allows poliovirus to utilize the host cell machinery exclusively for translation its own RNA, which is initiated by internal ribosome entry via a cap-independent mechanism. The functional role of the 2B, 2C and 2BC proteins are not much known. 2B, 2C, 2BC and 3CD proteins are involved in the replication complex of virus induced vesicles. All newly synthesized viral RNAs are linked with VPg. VPg is a 22 amino acid polypeptide which is derived from 3AB. The 3C and 3CD are protease and process most of the cleavage sites of the polyprotein precursor. The 3C protein is also involved in inhibition of RNA polymerase II and III mediated transcription by converting host transcription factor to an inactive form. The 3D is the RNA dependent RNA polymerase. It is known that poliovirus replication follows the general pattern of positive strand RNA virus. Plus strand RNA is transcribed into complementary minus strand RNA that, in turn, is transcribed for the synthesis of plus strand RNA is transcribed into complementary minus strand RNA that, in turn, is transcribed for the synthesis of plus strand RNA strands. Poliovirus RNA synthesis occurs in a membranous environment but how the template RNA and proteins required for RNA replication assemble in the membrane is not much known. The RNA requirements for the encapsidation of the poliovirus genome (packaging signal) are totally unknown. The poliovirus infection cycle lasts approximately 6 hours.

• Journal of Korean Academy of Nursing
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• v.45 no.3
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• pp.412-419
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• 2015

Purpose: The aim of this study was to evaluate the validity and reliability of the Korean version of the Person-centered Care Assessment Tool (P-CAT). Methods: The English P-CAT was translated into Korean with forward and backward translation. Survey data were collected from 458 staff in 17 long-term care facilities in Korea. Construct validity and criterion related validity were evaluated. Cronbach's alpha was used to assess reliability. Results: The Korean version of P-CAT was shown to be valid homogeneously by factor, item and content analysis. Internal consistency reliability was satisfactory in which the values of factor 1, factor 2 and the total scale were .84, .77 and .86 respectively. Exploratory factor analysis supported the construct validity with a two-factor solution. Factor loadings of the 13 items ranged in .34~.80. Criterion validity to the Person-centered Climate Questionnaire-staff (PCQ-S) was .74 (p<.001). Conclusion: The Korean version of the P-CAT was found to be an applicable instrument with satisfactory reliability and validity for further use in measuring successful person-centered care in long-term care facilities for older persons.

• Journal of Korean Academy of Nursing Administration
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• v.21 no.5
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• pp.542-551
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• 2015

Purpose: This study was done to evaluate validity and reliability of the Korean version of the expanded nursing stress scale (ENSS). Methods: Forward-backward translation of ENSS from English to Korean was conducted. The translated instrument was pilot-tested and administrated to 285 nurses who worked in five general hospitals in Korea. Internal consistency reliability was assessed using Cronbach's ${\alpha}$ and composite reliability. Validity was evaluated through construct validity with confirmatory factor analysis, convergent and discriminant validity. Results: The Korean version of ENSS showed a reliable internal consistency with Cronbach's ${\alpha}$ .95 and composite reliability .99. Model fit indexes for 46 items were validated by confirmatory factor analysis(goodness of fit index .82, Tucker-Lewis index .92, root mean square error of approximation .04), indicating suitable construct validity. Factor loading of the 48 items (.47~.88) and average variance extracted out of nine factors (.52~.71.) indicated satisfactory convergent validity. Some values of square of the correlation coefficient between factors (.12~.89) were higher than average variance extracted values of each factor (.52~.71) so that it partly satisfied discriminant validity. Conclusion: Findings show that the Korean version ENSS has good reliability and suitable validity; therefore, can be used to assess and identify Korean nurses' job stress.

• Korean Journal of Culture and Social Issue
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• v.29 no.1
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• pp.25-51
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• 2023

The purpose of the study was to validate the Korean version of the Heterophobia Scale for Gay Male (HGM). Heterophobia refers to the rejection, fear, and avoidance that sexual minorities feel against of heterosexuals. The three subscales of HGM assesses the unease/avoidance, disconnectedness, and expected rejection fromwith heterosexual male. To validate the Korean HGM, item translation, item analysis, and exploratory factor analysis were conducted. From the Using exploratory factor analysis of the Korean version of the HGM(N=213), per 13 items, a 3-factor model consisting of 13 items wereas determined. The Cconfirmatory factor analysis supported the goodness of fit of the 3-factor structure of the K-HGM (N=153) and showed adequate model fit, K-HGM showed adequate the internal consistency, convergent validity, discriminant validity and Criterion validity was good with other related scales (K-SOMS, K-MISS-G, SDS-9, and RS). In conclusion, the K-HGM is a valid measure of heterophobia for gay male within the Korean population. Based on the findings, implications, practical use, limitations, and suggestions for future study are discussed.

• Journal of Life Science
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• v.25 no.10
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• pp.1184-1195
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• 2015

The zebrafish is an emerging vertebrate model organism in reproductive biology. The oocyte maturation of zebrafish is triggered by maturation inducing hormone (MIH, 17α,20β-Dihydroxy-4-pregnen-3-one). In almost all animals, the oocyte maturation is governed by activation of pre-MPF which consists of cyclinB and inactive Cdk1. In the oocyte of Xenopus and mice, the activity of Cdk1 is regulated in two ways, one is the interaction with cyclinB and the other is phosphorylation/dephosphorylation of T14/Y15 residues on the Cdk1 by Wee1 and Cdc25. Unlike Xenopus and mice that have a sufficient amount of pre-MPF, pre-MPF is absent in GV oocyte of most teleost including zebrafish. Therefore, the activation of MPF during zebrafish oocyte maturation might totally depend on de novo synthesis of cyclinB proteins. It is reported that the translation of maternal mRNA is regulated by combination of several RNA binding proteins such as CPEB, Dazl, Pum1/Pum2, and insulin-like growth factor2 mRNA-binding protein 3 in the zebrafish oocytes. However, the definitive mechanism of these proteins to regulate the translation of stored maternal mRNAs remains to be elucidated. Therefore, the investigation of the maturation process of the zebrafish oocyte will provide new information that can help identify the role of translational control in early vertebrate oocyte maturation.

• Korean Journal of Plant Tissue Culture
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• v.28 no.5
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• pp.283-287
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• 2001

Differential display based on PCR was employed to identify genes expressed during tuber-developing stage of potato (Solanum tuberosum L. cv. Irish Cobbler). An eukaryotic initiation factor 5A (eIF-5A) clone isolated from a cDNA library constructed with developing micro-tuber using a probe of PCR fragment. We isolated three positive clones and ore of them contained open reading frame. This clone revealed high sequence similarity to tomato eIF 5A cDNA. At the DNA level, there is 94.8% identity with the tomato eIF-5A4, whereas at the protein level there is a high identity with 97.5%. The potato eIF 5A clone is 716 bp in length and contains a single open reading frame from 57 to 539 bp, a 56 bp 5'-untranslated region and a 177 bp 3'-untranslated region. The deduced protein composed of 160 amino acid residues, with a predicted molecular mass of 17.4 kD and an estimated pl of 5.5. The sequence of 12 (STSKTGKHGHAK) amino acids among eIF-5A proteins is perfectly conserved from yeast to human. That sequence in potato eIF-5A protein is also conserved at position 46 to 57 amino acid. This region embeds the post-translational modification site of the lysine residue (at the seventh K) to hypusine that is crucial to eIF-5A activity. The northern blot analysis of eIF5A has shown abundant expression, mainly in flower organs (stamen, ovary, petal, sepal), fruit and stolen.