• Title/Summary/Keyword: Transformation/Synthesis

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Transgenic Tobacco Plants Expressing the Bacterial Levansucrase Gene Show Enhanced Tolerance to Osmotic Stress

  • Park, Jeong-Mee;Kwon, Suk-Yoon;Song, Ki-Bang;Kwak, Ju-Won;Lee, Suk-Bae;Nam, Young-Woo;Shin, Jeong-Sheop;Park, Young-In;Rhee, Sang-Ki;Paek, Kyung-Hee
    • Journal of Microbiology and Biotechnology
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    • v.9 no.2
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    • pp.213-218
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    • 1999
  • Fructans are polyfructose molecules that function as nonstructural storage carbohydrates in several plants. In addition, it has been suggested that, due to their solubility, they can play an important role in helping plants survive periods of osmotic stress. In order to study the effect of levan synthesis on plant growth, the coding region of the levansucrase gene, which was isolated from Zymomonas mobilis, was introduced into tobacco plants using Agrobacterium tumefaciens-mediated transformation. The presence of the levansucrase gene in transgenic plants was verified by genomic DNA gel blot analysis. RNA gel blot and immunoblot analyses showed an accumulation of the corresponding transcript and protein product of the bacterial levansucrase gene in transgenic plants. Furthermore, a thin layer chromatography analysis revealed that fructans were synthesized and deposited in transgenic tobacco plants. When $T_1$ seeds were germinated and grown under polyethylene glycol-mediated drought stress or cold stress, the transgenic seedlings displayed a substantially higher level of growth than that of untransformed plants. These results suggest that fructans may playa significant role in the tolerance of plants under osmotic stress.

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Hydrothermal Synthesis of LaCO3OH and Ln3+-doped LaCO3OH Powders under Ambient Pressure and Their Transformation to La2O2CO3 and La2O3

  • Lee, Min-Ho;Jung, Woo-Sik
    • Bulletin of the Korean Chemical Society
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    • v.34 no.12
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    • pp.3609-3614
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    • 2013
  • Orthorhombic and hexagonal lanthanum(III) hydroxycarbonate ($LaCO_3OH$) and $Ln^{3+}$-doped $LaCO_3OH$ ($LaCO_3OH:Ln^{3+}$, where Ln = Ce, Eu, Tb, and Ho) powders were prepared by a hydrothermal reaction under ambient pressure and characterized by thermogravimetry, powder X-ray diffraction, infrared and luminescence spectroscopy, and field-emission scanning electron microscopy. The polymorph of $LaCO_3OH$ depended on the reaction temperature, inorganic salt additive, species of $Ln^{3+}$ dopant, and solvent. The calcination of orthorhombic $LaCO_3OH:Ln^{3+}$ (2 mol %) powers at $600^{\circ}C$ yielded a mixture of hexagonal and monoclinic $La_2O_2CO_3:Ln^{3+}$ powders. The relative quantity of the latter increased with decreasing ionic radius of the $Ln^{3+}$ dopant ion and increasing doping concentrations. On the other hand, the calcination of hexagonal $LaCO_3OH:Ln^{3+}$ (2 mol %) powders at $600^{\circ}C$ resulted in a pure hexagonal $La_2O_2CO_3:Ln^{3+}$ powder, regardless of the species of $Ln^{3+}$ ions (Ln = Ce, Eu, and Tb). The luminescence spectra of $LaCO_3OH:Ln^{3+}$ and $La_2O_2CO_3:Ln^{3+}$ were measured to examine the effect of their polymorph on the spectra.

A Study on the Synthesis of Co-ferrite by High-energy Ball Milling and Thermal Reduction Characteristics (고 에너지 볼 밀링을 통한 Co-ferrite 제조 및 열적 환원에 대한 연구)

  • Cho, M.S.;Kim, W.J.;Kim, C.H.;Kang, K.S.;Kim, Y.H.;Park, C.S.
    • Transactions of the Korean hydrogen and new energy society
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    • v.17 no.3
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    • pp.309-316
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    • 2006
  • Co-ferrite was synthesized by HEBM (High Energy Ball Milling) with a stoichiometric (Co/Fe=0.5/2.5) mixture of CoO and $Fe_2O_3$ powders. The effect of milling time on the phase transformation of the mixture was investigated by XRD. Mono-phase solid solution of Co-ferrite, which was milled for 4 h and then calcined at $900^{\circ}C$ in the Ar atmosphere, was confirmed by XRD analysis. The composition and thermal reduction behavior of Co-ferrite were analyzed by TGA and XRF. As a result, oxygen deficient Co-ferrite was synthesized by HEBM and the weight decrease of the Co-ferrite, which was oxidized at $600^{\circ}C$ for 10h by $H_2O$ vapor, was 2.41 wt% during thermal reduction at $1300^{\circ}C$.

Teaching Architectural Design in Post-War America - Gyorgy Kepes' Basic Course at MIT's Department of Architecture - (20세기 후반 MIT의 건축설계 교육과 기요르기 케피쉬의 기초디자인 프로그램의 특성과 변화에 관한 연구)

  • Pai, Hyung-Min
    • Journal of architectural history
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    • v.15 no.2
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    • pp.39-54
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    • 2006
  • Focusing on the emergence of the basic course in American schools of architecture, in particular Gyorgy Kepes' courses at MIT, this paper studies the transformation of architectural pedagogy during the years after World War II. Kepes centered his architectural pedagogy on the picture plane, which was to function as the primary media for applying the principles of Gestalt psychology, that is the identification of the whole and its parts and the reciprocity between the internal human organism and the outside world. Kepes hence introduced a set of unconventional visual practices that were not readily assimilated to architectural conventions. Paralleling the establishment of the basic course, MIT also formulated a functionalist and spatial pedagogy with its two initial design studios, courses 4.721 and 4.722. These studios shared the notion that architectural design evolved from the inside toward the outside, an idea that took hold not just in the pragmatic environment of MIT's studios but also in conservative academic programs as well as in popular magazines, picture books, and exhibitions for the consumer public. The architectural surface became inseparable from the objects of art, furniture, and design, all of which were to be the generators of space. Hence, during the 1950s, the architectural surface provided a specific locus of intersection between the visual fundamentals of the basic course and the working principles of architectural design. Kepes, however, had by this time become disillusioned with architecture's potential as the medium of unity. Though he maintained the Gestalt logic of identity, he expanded it toward the goal of grander synthesis of society and consciousness freeing himself from the constraints of disciplinary instruction. In the case of Kepes, the mediating role of the picture plane was foregone in a regressive turn toward a primal, innocent, and direct experience.

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Immobilization of Metal lons Using Low-Temperature Calcination Techniques of Spinel-ferrites

  • Yen, Fu-Su;Kao, Hsiao-Chiun;Chen, Wei-Chien
    • Proceedings of the IEEK Conference
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    • 2001.10a
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    • pp.106-110
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    • 2001
  • Formation of stoichiometric lithium-, nickel-, and zinc- ferrites by calcining organo-metallic precursors a temperature below 40$0^{\circ}C$ is examined using DTA/TG, and XRD techniques. It attempts to simulate th immobilization of metal ions in industrial liquid influents (waste) through the synthesis of stoichiometric spinel ferrites (SSF). Two steps of the SSF formation during thermal treatments are noted. The transformation of magnetite to ${\gamma}$ - Fe$_2$O$_3$and subsequent first formation of SSF were observed at temperatures ranging from 200 to 45$0^{\circ}C$. Th formation of cation-containing ${\gamma}$-Fe$_2$O$_3$and subsequent second formation of the ferrite occurred at temperature ranges of < 45$0^{\circ}C$ and 500 to $650^{\circ}C$, depending on the heating rate used. Then the temperature range of 200t 45$0^{\circ}C$ is critical to the performance of the technique, because a calcination at the range would lead to a complete formation of SSF, avoiding the occurrences of ${\gamma}$-Fe$_2$O$_3$and ion-containing ${\gamma}$-Fe$_2$O$_3$. If not, so $\alpha$-Fe$_2$O$_3$would occur. And annealing at temperature above $650^{\circ}C$ must be employed by which solid-state reactio of $\alpha$-Fe$_2$O$_3$with metal ions (possibly metal oxides) to form SSF can be conducted.

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Synthesis of Mullite Powder from Alkoxides and the Properties of the Mullite-Zircocnia Composites (알콕사이드로부터 Mullite 분말의 합성 및 Mullite-Zirconia 복합체의 특성)

  • 함종근;이홍림
    • Journal of the Korean Ceramic Society
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    • v.27 no.2
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    • pp.201-210
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    • 1990
  • The mullite-15v/o ZrO2 composites were prepared by dispersing ZrO2-3m/o Y2O3 powders into the mullite matrix in order to improve the mechanical properties of the mullite. The densification and retention of t-ZrO2 in the matrix of synthetic mullite were also investigated. From IR spectroscopic analysis, the obtained amorphous SiO2-Al2O3 powder was observed to have Si-O-Al chemical bond in its structure which might result in the homogeneous mullite composition. The lattice parameter of the mullite powder calcined above 130$0^{\circ}C$ (a0=7.5468$\AA$) is nearly close to the value of stoichiometric mullite (71.8wt% Al2O3, a0=7.5456$\AA$). The sintering behavior, microstructure, flexural strength and fracture toughness of the mullite and mullite-15v/o ZrO2 composites have been studied. The mullite-15v/o ZrO2(+3m/o Y2O3) ceramics with relative densities of 96% were obtained when sintered at 1$600^{\circ}C$. The flexural strength and fractrue toughness of the composites sintered at 1$600^{\circ}C$(calcination temperature of mullite powders ; 125$0^{\circ}C$) had maximum values of 307MPa and 2.50MPa.m1/2, respectively. The fracture toughness improvement in the mullite-ZrO2 cmoposite is assumed to be resulted from the combined effect of the stress-induced phase transformation of tetragonal ZrO2 and the crack deflection due to microcracking by the monoclinic ZrO2 formation.

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Analysis of Acoustic Propagation using Spectral Parabolic Equation Method (스펙트럴 포물선 방정식 법을 이용한 수중음파 전달해석)

  • Kim, Kook-Hyun;Seong, Woo-Jae
    • The Journal of the Acoustical Society of Korea
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    • v.15 no.2
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    • pp.72-78
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    • 1996
  • This thesis deals with a method to solve a two-and-one-half-dimensional ($2\frac12$ D) problem, which means that the ocean environment is two-dimensional whereas the source is fully three-dimensionally propagating, including three-dimensional refraction phenomena and three-dimensional back-scattering, using two-dimensional two-way parabolic equation method combined with Fourier synthesis. Two dimensional two-way parabolic equation method uses Galerkin's method for depth and Crank-Nicolson method and alternating direction for range and provides a solution available to range-dependent problem with wave-field back-scattered from discontinuous interface. Since wavenumber, k, is the function of depth and vertical or horizontal range, we can reduce a dimension of three-dimensional Helmholtz equation by Fourier transforming in the range direction. Thus transformed two-dimensional Helmholtz equation is solved through two-way parabolic equation method. Finally, we can have the $2\frac12$ D solution by inverse Fourier transformation of the spectral solution gained from in the last step. Numerical simulation has been carried out for a canonical ocean environment with stair-step bottom in order to test its accuracy using the present analysis. With this spectral parabolic equation method, we have examined three-dimensional acoustic propagation properties in a specified site in the Korean Straits.

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Synthesis and Characteristics of CU/CUO Nanopowders by Pulsed Wire Evaporativn(PWE) Method (전기폭발법에 의한 CU/CUO 나노분말의 제조 및 분말특성)

  • Maeng, D.Y.;Rhee, C.K.;Lee, N.H.;Park, J.H.;Kim, W.W.;Lee, E.G.
    • Korean Journal of Materials Research
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    • v.12 no.12
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    • pp.941-946
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    • 2002
  • Both Cu and Cu-oxide nanopowders have great potential as conductive paste, solid lubricant, effective catalysts and super conducting materials because of their unique properties compared with those of commercial micro-sized ones. In this study, Cu and Cu-oxide nanopowders were prepared by Pulsed Wire Evaporation (PWE) method which has been very useful for producing nanometer-sized metal, alloy and ceramic powders. In this process, the metal wire is explosively converted into ultrafine particles under high electric pulse current (between $10^4$ and $10^{ 6}$ $A/mm^2$) within a micro second time. To prevent full oxidations of Cu powder, the surface of powder has been slightly passivated with thin CuO layer. X-ray diffraction analysis has shown that pure Cu nanopowders were obtained at $N_2$ atmosphere. As the oxygen partial pressure increased in $N_2$ atmosphere, the gradual phase transformation occurred from Cu to $Cu_2$O and finally CuO nanopowders. The spherical Cu nanopowders had a uniform size distribution of about 100nm in diameter. The Cu-oxide nanopowders were less than 70nm with sphere-like shape and their mean particle size was 54nm. Smaller size of Cu-oxide nanopowders compared with that of the Cu nanopowders results from the secondary explosion of Cu nanopowders at oxygen atmosphere. Thin passivated oxygen layer on the Cu surface has been proved by XPS and HRPD.

The Lived Experience of Suffering of Family with Cancer Patients: Parse's Human Becoming Research Method (암환자 가족의 고통체험 - Parse의 인간되어감 연구방법 -)

  • Choi, Ye-Sook
    • Journal of Hospice and Palliative Care
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    • v.19 no.2
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    • pp.127-135
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    • 2016
  • Purpose: The purpose of this study was to discover the structure of the lived experience of suffering of families with cancer patients to develop a theoretical foundation that can be used to reinforce nursing practice for cancer patients and their families. Methods: A qualitative study was performed using Parse's research method. Participants were four families with cancer patients. From February 2009 through April 2010, data were collected via dialogical-engagement between participants and the researcher and analyzed through the extraction-synthesis and heuristic interpretation processes. Results: The structure was identified as follows. The families' lived experience of suffering was a process through which they experienced a psychological shock of cancer diagnosis and difficulties associated with reshuffled roles among family members, and made efforts to care for the patients. Conclusion: Amidst sadness, pain, anxiety, guilt, fear and agony, the families focused on the human-health-universe aspect and found meanings of their experiences as love, triumphant, responsibility and hope. As such, the study results suggest that the suffering of families with cancer patients is a human becoming process of positive transformation.

The Facile Synthesis of Pyrrolidinobenzenes from Adipaldehyde and Phenylenediamine using$HFe(CO)_4^-$ (사카르보닐철산염, $HFe(CO)_4^-$을 이용한 아디파알데히드와 페닐렌디아민으로부터 과수소아제피노벤젠의 편리한 합성)

  • Sang Chul Shim;Byung Won Woo;Chil Hoon Doh;Dong Yup Lee;Young Zoo Youn
    • Journal of the Korean Chemical Society
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    • v.36 no.2
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    • pp.301-304
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    • 1992
  • Ethanolic tetracarbonylhydridoferrate, $HFe(CO)_4^-$, combined with adipaldehyde is very efficient for the selective transformation of an amino group into a perhydroazepine ring. Phenylenediamines react with adipaldehyde in the presence of $HFe(CO)_4^-$at room temperature under the atmospheric pressure of carbon monoxide to give the corresponding perhydroazepines in moderate yields. In these reactions, the molar ratio of 1.0 : 1.0 : 1.0 of the ferrate-adipaldehyde-phenylenediamine system gave one perhydroazepine ring, and the case of 1.0 : 2.0 : 1.0 gave two perhydroazepine rings, selectively.

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