• Title/Summary/Keyword: Training Kit

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Performance comparison and evaluation of interferon-gamma assay kit for bovine tuberculosis diagnosis (소 결핵 진단을 위한 인터페론감마 검사 키트의 성능 비교 평가)

  • Hong, Leegon;Choi, Woojae;Ro, Younghye;Ahn, Sunmin;Kim, Eunkyung;Choe, Eunhee;Kim, Danil
    • Korean Journal of Veterinary Service
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    • v.43 no.4
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    • pp.201-209
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    • 2020
  • In Korea, bovine tuberculosis (bTB) is a representative zoonotic disease that causes considerable economic loss. In determining the positive bTB, the ELISA method for examining the amount of interferon-gamma (IFN-γ) is included in Korea's diagnostic standard method. Recently, commercially available BIONOTE TB-Feron ELISA Plus (TB-Feron Plus) that detects IFN-γ has been introduced. However, since the scientific basis for the performance is limited, we evaluated performance by comparing it with the results of another IFN-γ ELISA assay kit (BOVIGAM®) certified by Office International des Epizooties. In our research, 42 positive blood samples preliminarily tested with a tuberculin skin test and/or BOVIGAM® and 54 negative blood samples collected from three bTB free farms were subjected to IFN-γ assay using the TB-Feron Plus and the BOVIGAM®, respectively. The result shows that the sensitivity, specificity and accuracy were 81.0% (34/42), 100% (54/54), 91.7% (88/96) in TB-Feron Plus kit and 78.6% (33/42), 100% (54/54), 90.6% (87/96) in BOVIGAM® kit, respectively. Moreover, the overall accordance percentage of the two kits was 99.0% (95/96) and there was almost perfect agreement between two assays (Kappa=0.977, P<0.0001). Furthermore, additional studies confirmed that elevated lymphocyte numbers in blood did not interfere with the results of the TB-Feron Plus kit. And, delayed time from sampling to culture decreased the optical density (OD) value. Therefore, we concluded that the TB-Feron Plus kit was not inferior to BOVIGAM® in performance. High lymphocyte numbers in blood did not impact on TB-Feron Plus results, while delayed time before culture interfered with OD value.

Development of Rapid Immune-gold Strip Kit for On-Site Diagnosis of Tomato spotted wilt virus

  • Yoon, Ju-Yeon;Choi, Gug-Seoun;Cho, In-Sook;Choi, Seung-Kook
    • Research in Plant Disease
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    • v.20 no.1
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    • pp.15-20
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    • 2014
  • A rapid, user-friendly and simple immune-chromatographic dipstick kit named 'rapid immune-gold strip' (RIGS) kit was developed in a novel single strip format to detect on-site detection of Tomato spotted wilt virus (TSWV). Immunoglobulin G (IgG) from polyclonal antisera raised in rabbits against TSWV was purified through protein-A affinity chromatography and then the purified TSWV-IgG was conjugated to colloidal gold nano-particles which served as a test line on nitrocellulose membrane. Protein A that non-specifically binds to TSWV antibody was used as a control line on the same strip. The diagnosis process with the TSWV-RIGS involves simply grinding the suspect plant sample in a bag that contains the extraction buffer and inserting the strip the bag. Results can be seen in 2-5 minutes. The flow of the complexes of gold particles coated with TSWV-IgG and a crude sap from TSWV-infected pepper, tobacco and tomato plants resulted in intensive color formed on the test lines proportional to the concentrations of TSWV. The RIGS-TSWV kit did not show any cross-reactions against other tomato-infecting viruses unrelated to TSWV. These results indicate that the TSWV-RIGS kit is highly sensitive and is not required for laboratory training and experience prior to testing. The TSWV-RIGS kit is suitable for on-site detection of suspect TSWV-infected plants as well as for laboratory diagnosis.

A Study on Development of Educational CanSat based on Arduino for Creative Engineering Design and Practice Class (창의공학설계 및 실습 수업을 위한 아두이노 기반 교육용 캔위성 개발 연구)

  • Lee, Younggun;Lee, Sanghyun;Kim, Jongbum;Kim, Songhyon;Yoo, Seunghoon
    • Journal of Engineering Education Research
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    • v.24 no.5
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    • pp.38-45
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    • 2021
  • The CanSat was designed as an educational satellite simulation program that implements the overall system of the satellite such as the command processing unit, the communication unit, and the power unit in a structure of the size of a can. In particular, the training effect is very excellent because the trainee can learn a process similar to the actual satellite development process by designing, manufacturing, testing, and launching. Republic of Korea Air Force Academy has been using the CanSat production kit used by the domestic can satellite contest experience department for education, but since it was produced based on PCB, it was impossible to show creativity and operation was restricted even with small mistakes. In this paper, we analyze the existing CanSat kit and propose a new educational CanSat kit that can be used in creative engineering design and practice subjects that will be reorganized into a regular course from 2021, and a lesson plan. In conclusion, by using the proposed CanSat kit for lectures, it is possible to achieve educational purposes and effects, improve lecture satisfaction, and provide stable instruction.

Usability of CPR Training System based on Extended Reality (확장현실 기반의 심폐소생술 교육 시스템의 사용성 평가)

  • Lee, Youngho;Kim, Sun Kyung;Choi, Jongmyung;Park, Gun Woo;Go, Younghye
    • Journal of Internet of Things and Convergence
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    • v.8 no.6
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    • pp.115-122
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    • 2022
  • Recently, the importance of CPR training for the layperson has been emphasized to improve the survival rate of out-of-hospital cardiac arrest patients. An accurate and realistic training strategy is required for the CPR training effect for laypersons. In this study, we develop an extended reality (XR) based CPR training system and evaluate its usability. The XR based CPR training system consisted of three applications. First, a 3D heart anatomy image registered to the manikin is transmitted to the smart glasses to guide the chest compression point. The second application provides visual and auditory information about the CPR process through smart glasses. At the same time, the smartwatch sends a vibration notification to guide the compression rate. The 'Add-on-kit' is a device that detects the depth and speed of chest compression via sensors installed on the manikin and sends immediate feedback to the smartphone. One hundred laypersons who participated in this study agreed that the XR based CPR training system has realism and effectiveness. XR based registration technology will contribute to improving the efficiency of CPR training by enhancing realism, immersion, and self-directed learning.

A Study on the Korean Syllable As Recognition Unit (인식 단위로서의 한국어 음절에 대한 연구)

  • Kim, Yu-Jin;Kim, Hoi-Rin;Chung, Jae-Ho
    • The Journal of the Acoustical Society of Korea
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    • v.16 no.3
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    • pp.64-72
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    • 1997
  • In this paper, study and experiments are performed for finding recognition unit fit which can be used in large vocabulary recognition system. Specifically, a phoneme that is currently used as recognition unit and a syllable in which Korean is well characterized are selected. From comparisons of recognition experiments, the study is performed whether a syllable can be considered as recognition unit of Korean recognition system. For report of an objective result of the comparison experiment, we collected speech data of a male speaker and processed them by hand-segmentation for phoneme boundary and labeling to construct speech database. And for training and recognition based on HMM, we used HTK (HMM Tool Kit) 2.0 of commercial tool from Entropic Co. to experiment in same condition. We applied two HMM model topologies, 3 emitting state of 5 state and 6 emitting state of 8 state, in Continuous HMM on training of each recognition unit. We also used 3 sets of PBW (Phonetically Balanced Words) and 1 set of POW(Phonetically Optimized Words) for training and another 1 set of PBW for recognition, that is "Speaker Dependent Medium Vocabulary Size Recognition." Experiments result reports that recognition rate is 95.65% in phoneme unit, 94.41% in syllable unit and decoding time of recognition in syllable unit is faster by 25% than in phoneme.

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The Effects of a Coordinative Locomotor Training Program on the Functional Movement Screen Scores of Badminton Players (CLT 프로그램이 배드민턴 선수의 Functional Movement Screen 점수에 미치는 효과)

  • Kim, Tae-Yoon;Kim, Seok-Hwan
    • PNF and Movement
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    • v.14 no.1
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    • pp.23-32
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    • 2016
  • Purpose: The purpose of this study was to determine the effects of a coordinative locomotor training program on the functional movement screen (FMS) scores of badminton players. Methods: The participants consisted of 31 badminton players who were randomly assigned to either an experimental group (n=15) or a control group (n=16), and engaged in exercise five times per week for six weeks. The experimental group engaged in coordinative locomotor training and the control group engaged in general exercise. An FMS kit (USA) was used to measure the following: FMS score, deep squat, hurdle step, in-line lunge, shoulder mobility, active straight leg raise, trunk stability push up, and rotary stability. Results: The FMS score, deep squat, hurdle step, in-line lunge, active straight leg raise, and trunk stability push up showed significant improvement in the experimental group (p<0.05). Conclusion: The coordinative locomotor training program was able to produce confirmation that functional movement screen scores change in the case of effective exercise interventions in badminton players.

Performance Evaluation and Development of Virtual Reality Bike Simulator (가상현실 바이크 시뮬레이터의 개발과 성능평가)

  • Kim, Jong-Yun;Song, Chul-Gyu;Kim, Nam-Gyun
    • The Transactions of the Korean Institute of Electrical Engineers D
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    • v.51 no.3
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    • pp.112-121
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    • 2002
  • This paper describes a new bike system for the postural balance rehabilitation training. Virtual environment and three dimensional graphic model is designed with CAD tools such as 3D Studio Max and World Up. For the real time bike simulation, the optimized WorldToolKit graphic library is embedded with the dynamic geometry generation method, multi-thread method, and portal generation method. In this experiment, 20 normal adults were tested to investigate the influencing factors of balancing posture. We evaluated the system by measuring the parameters such as path deviation, driving velocity, COP(center for pressure), and average weight shift. Also, we investigated the usefulness of visual feedback information by weight shift. The results showed that continuous visual feedback by weight shift was more effective than no visual feedback in the postural balance control It is concluded this system might be applied to clinical use as a new postural balance training system.

Face-Mask Detection with Micro processor (마이크로프로세서 기반의 얼굴 마스크 감지)

  • Lim, Hyunkeun;Ryoo, Sooyoung;Jung, Hoekyung
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.25 no.3
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    • pp.490-493
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    • 2021
  • This paper proposes an embedded system that detects mask and face recognition based on a microprocessor instead of Nvidia Jetson Board what is popular development kit. We use a class of efficient models called Mobilenets for mobile and embedded vision applications. MobileNets are based on a streamlined architechture that uses depthwise separable convolutions to build light weight deep neural networks. The device used a Maix development board with CNN hardware acceleration function, and the training model used MobileNet_V2 based SSD(Single Shot Multibox Detector) optimized for mobile devices. To make training model, 7553 face data from Kaggle are used. As a result of test dataset, the AUC (Area Under The Curve) value is as high as 0.98.

Development of Weight Shifting Training System using Biofeedback for Post-stroke Hemiplegic Patients with Step Length Asymmetry (보폭 비대칭을 보이는 뇌졸중 후 편마비 환자를 위한 체중이동 훈련 시스템 개발)

  • Kim, Seeun;Kim, Deog Young;Kim, Jung Hoon;Choi, Jong Hyun;Joo, So Young;Kang, Na Kyung;Baek, Yoon Su
    • Journal of the Korean Society for Precision Engineering
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    • v.30 no.4
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    • pp.450-458
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    • 2013
  • The aim of this study was to develop and verify gait training system for post-stroke hemiplegia patients with step length asymmetry. Most post-stroke hemiplegic patients show gait asymmetry and weight shifting training has been suggested as a useful method for improving the walking ability. However, verbal cue by physical therapist may be not effective. Therefore, our weight shift training system was designed to give a feedback to patients through precise plantar pressure and center of pressure (COP) measurement. This weight shifting biofeedback training system is composed of F-Scan plantar pressure measurement system and software development kit (SDK) for Windows operating system. Two post-stroke patients with step length asymmetry were enrolled in this study. After training for six weeks, the weight shift score and step length ratio of two all patients were improved and approached to them of non-disabled. This system developed in this study may improve the step length asymmetry, and therefore this system is also expected to improve a walking ability in hemiplegic patients.

Use of Oral Antidiabetic Drugs (Metformin and Pioglitazone) in Diabetic Patients with Breast Cancer: How Does It Effect on Serum Hif-1 Alpha and 8Ohdg Levels?

  • Ece, Harman;Cigdem, Erten;Yuksel, Kucukzeybek;Ahmet, Dirican;Hakan, Er;Oktay, Tarhan Mustafa
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.10
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    • pp.5143-5148
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    • 2012
  • Objective: The aim was to investigate indicators related to DNA damage and cancer pathogenesis in Type II diabetes cases with breast cancer. It was planned to evaluate the relationship between these markers with oral antidiabetic drugs. Research Design and Methods: Fourty patients and 10 healthy individuals were included in the study. HIF-$1{\alpha}$ and 8-OHdG are examined in blood samples taken from these individuals with an ELISA Kit. Statistical analysis of data was performed with 95% confidence using Windows package program SPSS 15.0. Results: HIF-$1{\alpha}$ parameters were found to be meaningfully higher in the patient group than the controls in both pretreatment and posttreatment periods (p<0.05). No significant differences in terms of 8-OHdG between patients and controls. However, posttreatment serum HIF-$1{\alpha}$ ve 8-OHdG levels was found lower than pretreatment levels in patients receiving metformin, but not with pioglitazone. Conversely, serum 8-OHdG levels decreased significantly in these patients. When patients were evaluated according to the treatment groups (pioglitazone vs. metfformin) no significant differences in terms of serum HIF-$1{\alpha}$ and 8-OHdG levels between treatment groups. Conclusions: HIF-$1{\alpha}$ levels decreased significantly in the patient group receiving metformin. However, there was no significant difference in terms of HIF-$1{\alpha}$ levels in the patients receiving pioglitazone.