• Title/Summary/Keyword: Tracer-test

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Development and Evaluation of a Competitive Enzyme Immunoassay for the Detection of Antibodies to Treponema pallidum (경쟁적 효소면역 측정법을 이용한 Treponema pallidum 항체 진단시약의 개발 및 평가)

  • 김병문;이정환;정문섭;김승철;이미용;이성희;김원배
    • Microbiology and Biotechnology Letters
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    • v.27 no.4
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    • pp.344-348
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    • 1999
  • A competitive enzyme-linked immunosorbent assay(ELISA) for the detection of antibodies to Treponema pallidum(T.pallidum) was developed and evaluated. T.apllidum lysate was immobilized on the surface of microplate wells and horseradish peroxidase labeled human anti-T.pallidum lysate was immobilized on the surface of microplate wells and horseradish peroxidase labeled human anti-T.pallidum was prepared and used as a tracer. The performance of the competitive ELISA was evaluated by using different specimens. The competitive ELISA showed a sensitivity of 100% in a performance panel consisting of serum and plasma with anti-T.pallidum reactivity ranging from negative to strong positive by FTA-ABS test system and 120 plasma samples positive by TPHA. The specificity of the competitive ELISA was 100% in 1,200 plasma samples collected from healthy seronegative blood donors. These results suggest that the competitive ELISA provides an excellent assay method for the detection of antibodies to T.pallidum, and may be particularly useful for serological blood screening of syphilis.

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Development of X-ray PIV System Using a Medical X-ray Tube (임상용 X-선관을 이용한 X-ray PIV시스템의 개발)

  • Yim, Dae-Hyun;Kim, Guk-Bae;Kim, Do-Il;Lee, Hyong-Koo;Lee, Sang-Joon
    • 유체기계공업학회:학술대회논문집
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    • 2006.08a
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    • pp.403-406
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    • 2006
  • A new medical X-ray PIV technique was developed using a conventional medical X-ray tube. To acquire images of micro-scale particles, the X-ray PIV system consists of an x-ray CCD camera with high spatial resolution, and a X-ray tube with small a focal spot. A new X-ray exposure control device was developed using a rotating disc shutter to make double pulses which are essential for PIV application. Synchronization methodology was also developed to apply the PIV technique to a conventional medical X-ray tube. In order to check the performance and usefulness of the developed X-ray PIV technique, it was applied to a glycerin flow in an opaque silicon tube. Tungsten particles which have high X-ray absorption coefficient were used as tracer particles. Through this preliminary test, the spatial resolution was found to be higher than ultrafast MRI techniques, and the temporal resolution was higher than conventional X-ray PIV techniques. By improving its performance further and developing more suitable tracers, this medical X-ray PIV technique will have strong potential in the fields of medical imaging or nondestructive inspection as well as diagnosis of practical thermo-fluid flows.

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Decontamination Performance Assessment for the Plasma Arc Vitrification pilot plant on the basis of Trial Burn Results(I) - Decontamination Characteristics for Hazardous Metal, Radioactive surrogate and Radioactive Tracer in Off-gas (시험연소결과에 근거한 플라즈바 아크방식 유리화 시험 설비의 제염성능 평가(I) - 배기가스중의 유해중금속, 방사성핵종 모의물질 및 방사성핵종 제염특성 -)

  • Chae, Gyung-Sun;Park, Youn-Hwan;Min, Byong-Yun;Chang, Jae-Ock;Park, Jun-Yong;Jeong, Weon-Ik;Moon, Byung-Sik
    • Journal of Radiation Protection and Research
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    • v.25 no.2
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    • pp.99-107
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    • 2000
  • Through the results of off-gas analysis at 3 sampling points in Plasma Arc Melting vitrification pilot plant, it was evaluated the partitioning of spiked materials in off-gas and the decontamination characteristic of off-gas treatment system. Spiked materials are hazard_us heavy metals(Pb, Cd, Hg), radioactive surrogate(Co, Cs) and radioactive materials($^{60}Co,\;^{137}Cs$). Through the Trial burn tests, Decontamination factor of spiked materials in off-gas treatment system is calculated.

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A Study on Flow Characteristics of Polluted Air in Rectangular Tunnel Models Using a PIV System

  • Koh, Young-Ha;Park, Sang-Kyoo;Yang, Hei-Cheon;Lee, Yong-Ho
    • Journal of Advanced Marine Engineering and Technology
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    • v.34 no.6
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    • pp.825-832
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    • 2010
  • The objective of this study is to investigate flow behaviors of polluted air in order to prevent the impact of disaster in a tunnel. This paper presents the experimental results qualitatively in terms of flow characteristics in two kinds of rectangular tunnel models in which each distance from the centerline above the inlet vent to the exhaust vent is 0 and 60 mm, respectively. The olive oil is used as the tracer particles. The flow is tested at the flow rate of $14.16{\times}10^{-4}\;m^3/s$ and the inlet vent velocity of 1.1 m/s with the kinematic viscosity of air. The aspect ratio of the model test section is 10. The average velocity vectors, streamlines, and vorticity distributions are measured and analyzed by the Flow Manager in a particle image velocimetry(PIV) system. The PIV technology gives three different velocity distributions according to observational points of view for understanding the polluted air flow characteristics. The maximum value of mean velocity generally occurs in the inlet and outlet vent regions in the tunnel models.

자유면대수층내 포화대와 비포화대에서의 수리분산특성 연구

  • 강동환;정상용;이민희;김병우;이승엽;손주형
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2003.04a
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    • pp.168-171
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    • 2003
  • 연속주입추적자시험이 실시된 현장사이트의 규모는 4.5m$\times$4.5m$\times$6.0m 정도이다. 시험 사이트내에서 실시된 추적자시험은 주입공(Pl)에서 Rhodamine WT 50ppm 용액을 1.8$\ell$/min의 주입율로 6일 동안 연속적으로 주입함과 동시에 관측공(P2, P3, Il, I2, I3)에서 일정한 시간간격으로 지하수를 채수하여 추적자농도를 산출하였다. 시험결과, 지하수면 하부를 포함하는 관측공(P2, P3)의 최대추적자농도는 초기주입농도의 10% 정도이며, 지하수면 상부에 위치한 지하수공(Il, I2, I3)들에서의 최대추적자농도는 초기농도의 75% 정도로서 추적자의 농도차이가 상대적으로 매우 크게 나타났다. 본 연구에서는 자유면대수층내에서 포화대를 포함한 관측공과 비포화대만을 포함한 관측공에서의 수리분산특성에 대해 비교.분석한 결과, 오염물이 연속적으로 토양에 유입되는 경우 비포화대 구간에서는 이류기작에 의한 농도희석이 거의 없으므로 오염물농도가 매우 클 것으로 판단된다.

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Modeling High Power Semiconductor Device Using Backpropagation Neural Network (역전파 신경망을 이용한 고전력 반도체 소자 모델링)

  • Kim, Byung-Whan;Kim, Sung-Mo;Lee, Dae-Woo;Roh, Tae-Moon;Kim, Jong-Dae
    • The Transactions of the Korean Institute of Electrical Engineers D
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    • v.52 no.5
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    • pp.290-294
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    • 2003
  • Using a backpropagation neural network (BPNN), a high power semiconductor device was empirically modeled. The device modeled is a n-LDMOSFET and its electrical characteristics were measured with a HP4156A and a Tektronix curve tracer 370A. The drain-source current $(I_{DS})$ was measured over the drain-source voltage $(V_{DS})$ ranging between 1 V to 200 V at each gate-source voltage $(V_{GS}).$ For each $V_{GS},$ the BPNN was trained with 100 training data, and the trained model was tested with another 100 test data not pertaining to the training data. The prediction accuracy of each $V_{GS}$ model was optimized as a function of training factors, including training tolerance, number of hidden neurons, initial weight distribution, and two gradients of activation functions. Predictions from optimized models were highly consistent with actual measurements.

Development of Mathematical Model for Both Solute Transport in Snow and Isotopic Evolution of Snowmelt (눈 속에서의 용질이동 및 융설의 동위원소변동에 관한 모델개발)

  • Lee, Jeonghoon
    • Journal of Soil and Groundwater Environment
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    • v.17 no.5
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    • pp.31-39
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    • 2012
  • Chemical and isotopic variations of snowmelt provide important clues for understanding snowmelt processes and the timing and contribution of snowmelt to catchment or watershed in spring. The newly developed model includes a hydraulic exchange between mobile and immobile water (${\omega}$), and isotopic exchanges between both mobile water and ice ($f_1$) and immobile water and ice ($f_2$). Since the new model is based on the mobile-immobile water conceptualization, which is widely used for describing chemical tracer transport in snow, it allows simultaneous calculations of chemical as well as isotopic variations in snowpack discharge. We compare the model results with a study of solute transport and isotopic evolution of snowmelt in snow, using artificial rain-on-snow experiments with conservative anion ($Br^-$). These observations are used to test the newly developed model and to better understand physical processes in a seasonal snowpack where our model simulates the chemical and isotopic variations.

Functional Neuroimaging in Epilepsy: FDG-PET and SPECT (간질에서의 기능적 뇌영상:양전자방출단층촬영과 단일광전자방출 단층촬영)

  • Lee, Sang-Kun;Lee, Dong-Soo
    • The Korean Journal of Nuclear Medicine
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    • v.37 no.1
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    • pp.24-33
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    • 2003
  • Finding epileptogenic zone is the most important step for the successful epilepsy surgery. F-18 fluorodeoxyglucose positron emission tomography (FDG-PET) and single photon emission computed tomography (SPECT) can be used in the localization of epileptogenic foci. In medial temporal lobe epilepsy, the diagnostic sensitivity of FDG-PET and ictal SPECT is excellent. However, detection of hippocampal sclerosis by MRI is so certain that use of FDG-PET and ictal SPECT in medial temporal lobe epilepsy is limited for some occasions. In neocortical epilepsy, the sensitivities of FDG-PET or ictal SPECT are fair. However, FDG-PET and ictal SPECT can have a crucial role in the localization of epileptogenic foci for non-lesional neocortical epilepsy. Interpretation of FDG-PET has been recently advanced by voxel-based analysis and automatic volume of interest analysis based on a population template. Both analytical methods can aid the objective diagnosis of epileptogenic foci. Ictal SPECT was analyzed using subtraction methods and voxel-based analysis. Rapidity of injection of tracers, ictal EEG findings during injection of tracer, and repeated ictal SPECT were important technical issues of ictal SPECT. SPECT can also be used in the evaluation of validity of Wada test.

MODELING OF A BUOYANCY-DRIVEN FLOW EXPERIMENT IN PRESSURIZED WATER REACTORS USING CFD-METHODS

  • Hohne, Thomas;Kliem, Soren
    • Nuclear Engineering and Technology
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    • v.39 no.4
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    • pp.327-336
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    • 2007
  • The influence of density differences on the mixing of the primary loop inventory and the Emergency Core Cooling (ECC) water in the downcomer of a Pressurised Water Reactor (PWR) was analyzed at the ROssendorf COolant Mixing (ROCOM) test facility. ROCOM is a 1:5 scaled model of a German PWR, and has been designed for coolant mixing studies. It is equipped with advanced instrumentation, which delivers high-resolution information for temperature or boron concentration fields. This paper presents a ROCOM experiment in which water with higher density was injected into a cold leg of the reactor model. Wire-mesh sensors measuring the tracer concentration were installed in the cold leg and upper and lower part of the downcomer. The experiment was run with 5% of the design flow rate in one loop and 10% density difference between the ECC and loop water especially for the validation of the Computational Fluid Dynamics (CFD) software ANSYS CFX. A mesh with two million control volumes was used for the calculations. The effects of turbulence on the mean flow were modelled with a Reynolds stress turbulence model. The results of the experiment and of the numerical calculations show that mixing is dominated by buoyancy effects: At higher mass flow rates (close to nominal conditions) the injected slug propagates in the circumferential direction around the core barrel. Buoyancy effects reduce this circumferential propagation. Therefore, density effects play an important role during natural convection with ECC injection in PWRs. ANSYS CFX was able to predict the observed flow patterns and mixing phenomena quite well.

Dynamic Characteristics of Thermal Stratification Build-up by Unsteady Natural Convection (비정상 자연대류에 의한 온도성층화의 동특성에 관한 연구)

  • Kang, B.S.;Lee, J.S.;Lee, T.S.;Ro, S.T.
    • The Magazine of the Society of Air-Conditioning and Refrigerating Engineers of Korea
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    • v.17 no.4
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    • pp.382-394
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    • 1988
  • Dynamic characteristics of thermally-forced stratification process in a square enclosure with a linear temperature profile at the side walls have been investigated through flow visualization experiment and numerical analysis. The experiment was performed on air with the Rayleigh numbers of order $10^5$. A particle tracer method is used for the flow visualization and to obtain a sudden linear temperature profile at the side walls copper blocks which already have a linear temperature profile are come into contact with the thin copper plates of the test section. Immediately a meridional circulation is developed and heat transfer takes place from the wall to the interior region by circulation of fluid and finally a thermal stratification is achieved. In the numerical study, QUICK scheme for convective terms, SIMPLE algorithm for pressure correction, and the implicit method for the time marching are adopted for the integration of conservation equations. Comparison of flow visualization and numerical results shows that the developing flow patterns are very similar in dynamic nature even though there is a time lag due to the inevitable time delay in setting up a linear temperature profile. For high Rayleigh numbers, the oscillatory motion is likely to take place and stratified region is extended. However, initial temperature adjustment process is much slower than that for low Rayleigh numbers.

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