• Title/Summary/Keyword: Toxicity identification

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Identification and Molecular Characterization of Insecticidal cryl-type Genes from Bacillus thuringiensis 2385-1

  • Li, Ming-Shun;Park, Jae-Young;Roh, Jong-Yul;Shim, Hee-Jin;Boo, Kyung-Saeng;Je, Yeon-Ho
    • Proceedings of the Korean Society of Sericultural Science Conference
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    • 2003.10a
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    • pp.114-115
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    • 2003
  • A Bacillus thuringiensis isolate, Bt 2385-1, which showed toxicity to lepidopteran, was isolated from Korean soil sample and characterized. PCR-RFLP showed that this isolate contains two novel cryl-type crystal protein genes. In this study, we designed cryl-type specific primer set (ATG1-F and N400-R) to clone the toxic domain of the all cryl-type genes. The two novel rlyl-type toxin genes in addition to crylJal gene were cloned and sequenced. (omitted)

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Synthesis, Characterization and Identification of In Vitro and In Vivo DNA adducts of 1- and 2-Bromopropane

  • Moon, Yoon-Soo;Basnet, Arjun;Zhao, Long-Xuan;Kim, Eun-Kyung;Lim, Hyun-Tae;Chae, Whi-Gun;Jeong, Tae-Cheon;Lee, Eung-Seok
    • Proceedings of the PSK Conference
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    • 2003.04a
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    • pp.246.2-247
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    • 2003
  • It has been reported that 2-bromopropane might be a causative agent for reproductive toxicity and have immunotoxic effects. 1-Bromopropane known as an alternative to ozone depleting solvents, which has structural similarity to 2-bromopropane, has been reported to be neurotoxic to rats in long-term inhalation exposure. (omitted)

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Studies on the Pharmacological Actions of Cactus:Identification of Its anti-Inflammatory Effect

  • Park, Eun-Hee;Kahng, Ja-Hoon;Paek, Eun-Ah
    • Archives of Pharmacal Research
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    • v.21 no.1
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    • pp.30-34
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    • 1998
  • The ethanol extracts of Opuntia ficus-indica fructus (EEOF) and Opuntia ficus-indica stem (EEOS) were prepared and used to evaluate the pharmacological effects of cactus. Both the extracts inhibited the writhing syndrome induced by acetic acid, indicating that they contains analgesic effect. The oral administrations of EEOF and EEOS suppressed carrageenan-induced rat paw edema and also showed potent inhibition in the leukocyte migration of CMC-pouch model in rats. Moreover, the extracts suppressed the release of $\beta$-glucuronidase, a lysosomal enzyme in rat neutrophils. It was also noted that the extracts showed the protective effect on gastric mucosal layers. From the results it is suggested that the cactus extracts contain anti-inflammatory action having protective effect against gastric lesions.

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Identification of Novel Crystal Protein Gene from a Strain of Bacillus thuringiensis subsp. kenyae

  • Park, Jae-Young;Roh, Jong-Yul;Li, Ming-Shun;Shim, Hee-Jin;Boo, Kyung-Saeng;Je, Yeon-Ho
    • Proceedings of the Korean Society of Sericultural Science Conference
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    • 2003.04a
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    • pp.73-73
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    • 2003
  • Bacillus thuringiensis 2385-1, which showed toxicity to lepidopteran but not to dipteran was isolated from Korean soil sample and characterized. The H-serotype of 2385-1 was identical to that of serovar kenyae (H4a4c), and its crystal toxin was bipyramidal-shaped with a molecular weight of 130 kDa. However, the plasmid profile of 2385-1 was different from that of serovar kenyae. (omitted)

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Studies on the Toxic Substances in Korean Barley Infected with Gibberella Zeae(I) Identification of the Toxicity of the Infected Grain by Animal Tests (적미병 보리의 유독성분에 관한 연구 (제 I 보) 동물실험에 의한 유독성의 확인)

  • Kim, Tae-Bong;Hahn, Bo-Sup;Hahn, Sang-Heyun;Kee, Ryong-Suk
    • Journal of the Korean Chemical Society
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    • v.8 no.2
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    • pp.75-77
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    • 1964
  • It was confirmed that korean scabby barley during the summer of 1963 was infected with Gibberella zeae. Feeding experiments showed that the diseased grain was toxic and induced emesis in pigs. The concentrated extracts which contained the emetic principle were prepared by refluxing the grain with methanol or water for 5 hours and then concentrating the filtrates and the washing in vacuum. Animal tests proved that methanol extraction for the emetic principle was more effective than extraction with water.

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Identification of Proteineous Biomarkers for Cadmium- and Ceramide- Induced Toxicity in Human Brain Cells through Display Proteomic Analysis

  • Oh, Mi-Jung;Chae, Kyu-Young;Park, Mi-Ja;Cho, Dong-Hawn;Kim, Dae-Kyong
    • Proceedings of the PSK Conference
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    • 2003.10b
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    • pp.104.2-104.2
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    • 2003
  • Cadmium is an environmental pollutant and exhibits nephrotoxicity, hepatotoxicity and immunotoxicity. Recently, cadmium was found to induce DNA fragmentation, a biochemical hallmark of apoptosis, in cultured renal cells, hepatocytes and neuroblastoma cell. Therefore, the various toxicities of cadmium are thought to be caused by the induction of apoptosis. Lipids-derived pro-apoptotic ceramide has emerged as an important intracellular signaling molecule that mediates diverse cellular effects, of which programmed cell death, or apoptosis, has attracted significant interest. (omitted)

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Mitophagy stimulation as a novel strategy for the treatment of mitochondrial diseases

  • Kang-Min Lee;Jeanho Yun
    • Journal of Genetic Medicine
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    • v.19 no.2
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    • pp.49-56
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    • 2022
  • Mitophagy, the selective degradation of damaged or surplus mitochondria using core autophagy machinery, plays an essential role in maintaining cellular mitochondrial function. Impaired mitophagy is closely linked to various human diseases, including neurodegenerative diseases, cardiovascular diseases, cancers and kidney disease. Defective mitophagy induces the accumulation of damaged mitochondria and thereby results in a decline in cellular survival and tissue function. Accordingly, enhancement of mitophagy has been proposed as a novel strategy for the treatment of human diseases closely linked to mitochondrial dysfunction. Recent studies showing that the stimulation of mitophagy has a therapeutic effect on several disease models highlight the possibility of disease treatment using mitophagy. The development of mitophagy inducers with toxicity and the identification of molecular mechanisms will enable the clinical application of mitophagy-based treatments.

The Comparative Study of Oriental Medicine in Korea, Japan and China (한국(韓國)과 일본(日本) 및 중국(中國)의 동양의학(東洋醫學)에 대한 비교연구(比較硏究))

  • Cho, Ki-Ho
    • The Journal of Korean Medicine
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    • v.19 no.1
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    • pp.271-298
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    • 1998
  • During these days of new understanding, western medicine has developed remarkably and a revaluation of traditional medicine has been achieved. This appears to have resulted from the sound criticisms of what western medicine has achieved up to now; excessive subdivisions of clinical medicine, severe toxicity of chemical drugs, lack of understanding about patients complaints which cannot be understood objectively, and etc. It is thought that the role of traditional medicine will be more important in the future than it is now. Someone said that the research methods of traditional medicine depends on the way of experimental science too much. That there was no consideration of a system for traditional medicine and the critic also went so far as to assert that in some cases the characteristics of eastern ideas is to permit irrationalism itself. In view of this thinking, the term traditional medicine seems to have been used somewhat too vaguely. However, traditional medicine is a medical treatment which has existed since before the appearance of modern medicine and it was formed from a traditional culture with a long history. One form of traditional medicine, oriental medicine based upon ancient Chinese medicine, was received in such countries as Korea, Japan, Thailand, Vietnam, Tibet, and Mongolia. Oriental medicine then developed in accordance with its own environment, race, national characteristics, and history. Although there are some simultaneous differences between them, three nations in Eastern Asia; Korea, Japan, and China, have especially similar features in their clinical prescriptions and medical literature. These three nations are trying to understand each others unique traditional medicines through numerous exchanges. Even though many differences in their ways of studying have developed over history exist, recent academic discussions have been made to explore new ways into oriental medicine. Therefore a comparative study of oriental medicine has gradually been thought to be more important. In Korea the formation of a new future-oriented paradigm for oriental medicine is being demanded. The purpose of the new paradigm is to create a new recognition of traditional culture which creates an understanding of oriental medicine to replace the diminished understanding of oriental medicine that was brought about by the self-denial of traditional culture in modem history and cultural collisions between oriental and occidental points of view. Therefore, to make a new paradigm for oriental medicine which is suitable for these days, and fortifies the merit of oriental medicine while compensating its defects, the author has compared the characteristics of oriental medicines in Korea, Japan, and China. The conclusions of this research are as follows: 1. The fundamental differences of the traditional medicines of these three nations are caused by the differences in the systems of Naekyung and Sanghannon. 2. The pattern-identification of illnesses is generally divided into two categories; the pattern identification of Zang-Fu and the pattern identification of prescription. 3. There are many differences in the definition of terms, such as Yin and Yang, Deficiency and Excess, and etc. 4. Chinese traditional medicine has some new concepts about pattern identification and epidemic febrile disease. 5. Japanese traditional medicine has some characteristics about pattern identification of the whole bodys condition and signs of abdominal palpation. 6. In terms of the effects of herbal drugs, Chinese traditional medicine attaches great importance to the experiential efficacy of the herb, and Japanese traditional medicine is taking a serious view of the effects of experimental medical actions.

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Monitoring and Environmental Risk Assessment of Genetically Modified Microalgae (유전자변형 미세조류의 생태 유출 모니터링 및 위해성평가 연구)

  • Cho, Kichul;Jeon, Hancheol;Hwang, Hyun-Ju;Hong, Ji Won;Lee, Dae-Sung;Han, Jong Won
    • Journal of Marine Bioscience and Biotechnology
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    • v.11 no.2
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    • pp.52-61
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    • 2019
  • Over the past few decades, microalgae-based biotechnology conjugated with innovative CRISPR/Cas9-mediated genetic engineering has been attracted much attention for the cost-effective and eco-friendly value-added compounds production. However, the discharge of reproducible living modified organism (LMO) into environmental condition potentially causes serious problem in aquatic environment, and thus it is essential to assess potential environmental risk for human health. Accordingly, in this study, we monitored discharged genetically modified microalgae (GMM) near the research complex which is located in Daejeon, South Korea. After testing samples obtained from 6 points of near streams, several green-colored microalgal colonies were detected under hygromicin-containing agar plate. By identification of selection marker genes, the GMM was not detected from all the samples. For the lab-scale environmental risk assessment of GMM, acute toxicity test using rotifer Brachionus calcyflorus was performed by feeding GMM. After feeding, there was no significant difference in mortality between WT and transformant Chlamydomonas reinhardtii. According to further analysis of horizontal transfer of green fluorescence protein (GFP)-coding gene after 24 h of incubation in synthetic freshwater, we concluded that the GFP-expressed gene not transferred into predator. However, further risk assessments and construction of standard methods including prolonged toxicity test are required for the accurate ecological risk assessment.

Preliminary Risk Assessment of Several Major Pharmaceutical Products In Aquatic Ecosystem

  • Park, Su-Jung;Oh, So-Rin;Jung, Jin-Yong;Kim, Young-Hee;Kim, Pan-Gyi;Choi, Kyung-Ho
    • Proceedings of the Korean Environmental Health Society Conference
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    • 2005.06a
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    • pp.345-350
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    • 2005
  • Acute toxicities of five pharmaceutical products were evaluated with aquatic microbes, invertebrates, and fish. The test pharmaceuticals, i.e., cimetidine, carbamazepine, diltiazem, acetaminophene, and metformin have been often detected in aquatic environment, but theire cological hazard on receptors of various trophic levels has seldom been evaluated. In the present study, we conducted acute toxicity assays with a marine bacterium, Vibrio fischeri, an invertebrate, Daphnia magna, and a fish, Japanese medake (Oryzias latipes). In general, D. magna, showed the most sensitive response to the test chemicals. Diltiazem exhibited the lowest EC50 value after 96 hr of exposure at 7.6 mg/L, followed by cimetidine >acetaminophen > metformin = carbamazepine in an order of decreasing susceptibility. With the fish, diltiazem and carbamazepine showed the 96 hr EC50 values at 14.1${\sim}$35.4 mg/L while acetaminophen, cimetidine, and metformin did not cause 50% mortality at 100 mg/L. Similar pattern was noted with the Microtox Assay, with which the median effective concentrations for acetaminophen, cimetidine, and metformin were found at the range between 301.8 and 755.4 mg/L. Carbamazepine and diltiazem exposure to the microbes resulted in EC50 values around 50 mg/L. Predicted no effect concentrations (PECs) of these pharmaceuticals derived from the EC5O values obtained from this study, and predicted environmental concentrations (PECs) obtained from available literatures were utilized to estimate ecological risks of the test compounds. No test pharmaceuticals resulted in risk quotients (PEC/PNEC) greater than 1, which suggests no serious potential ecological concerns. It should be noted however that further studies including the refinement of PEC derivation, identification and toxicity assessment of the metabolites and/or their interactions with other stressors may be warranted to better understand the environmental consequences of the residual pharmaceutical discharge to the waterway.

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