• Korean Journal of Agricultural Science
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• v.33 no.1
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• pp.25-34
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• 2006

Two experiments were conducted to investigate the effects of molasses supplementation to silage materials in ensiling Galgeuntang (herbal medicine) meal on the quality of silage, and its palatability in Korean native goats. In experiment I, molasses was added at the levels of 0, 0.5, and 1.0% to Galgeuntang meal with three replicates per treatment. In experiment II, three 1.5-yr-old Korean native goats were used to measure palatability. In experiment I, lactic acid contents in molasses treatments were significantly(p<0.05) higher, and pH and butyric acid contents were lower than those of non-molasses treatment. In addition, molasses treatment increased total microbial cell counts in MRS medium for lactobacillus, but decreased total microbial cell counts in PDA medium for fungi. Molasses supplementation to silage materials increased in vitro dry matter disappearance. Molasses supplementation tended to increase silage intake in Korean native goat (experiment II), but the difference was not significant(p>0.05). It is concluded that molasses supplementation to silage materials in ensiling herbal medicine meal could improve its preservability and palatability, the higher the better.

• Journal of the Korean Society of Food Science and Nutrition
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• v.42 no.1
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• pp.148-152
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• 2013

An ultraviolet (UV) sterilization system was developed to decrease the number of microorganisms in filtered Acer mono sap (FAS). The Acer mono sap (AS) was passed through 18 strips of PTA fluoroplastic tubing with 30 UV lamps (total 1,170 W). During passage, the AS was effectively exposed to the UV rays. The total bacteria, coliform group and fungi were sterilized at a flow rate ranging from 852 to 1,358 mL/min and a UV power higher than 156 W. Although the sensory score of the UV-sterilized AS was significantly lower than that of the untreated AS, the sensory score was better under the condition of 390 W and 852~1,358 mL/min than under the other conditions examined. ANOVA did not reveal a significant difference in pH, total acidity, sugar content and color characteristic under all conditions tested (p<0.05). Considering the overall attributes of the AS quality, the optimum sterilization condition was determined to be 390 W and 852~1,358 mL/min.

• Journal of Preventive Medicine and Public Health
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• v.21 no.1 s.23
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• pp.31-46
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• 1988

In order to designate a present status necessary for establishment of preventive measures and guidelines of health education against hepatitis B in the course of secondary school education, knowledge and practice toward hepatitis B virus infection was surveyed by a questionnare method on total of 4,855 college entrants in the academic year of 1987 and analyzed the data collected using IBM PC(Trigem 88-II) with SAS package program. About two per cent of college entrants had past history of HBV infections not showing any difference between both sexes and geographical regions. About one third(33.7%) of total students had tested hepatitis B surface antigen(HBsAg), only 4% had tested hepatitis B surface antibody(HBsAb) and vaccination rate amounted to 24.6%, one fourth of total subjects. Both serological tests and vaccination were most commonly performed during adolescence, showing higher rates in female students than in male students. The rates also seemed to be higher in those from urban cities than those from rural cities. Students who had acquired correct knowledge that hepatitis B was infected by virus were amounted to 78.5% of college entrants, and remaining 21.5% had misunderstood that rickettsia, bacteria, fungi or parasites were causal agents. Female students were better aware of the causal agents than male students but there was no difference between places of growth. As for mode of transmission of HBV, 51.5% of male students and 47.7% of female students had correct knowledge. A very few student had known that fact that HBV was transmitted by body fluids such as tear(6.9%), nasal discharge(10.1%) and semen or vaginal secretion(19.2%) and majority(75%) of students had misunderstood that hepatitis B virus would be transmitted per os through food ingestion. Approximately one half(48.9%) of college entrants had knew correctly whom to be vaccinated. Approximately one half of the students knew that hepatr;ma(57.8%) and liver cirrhosis(57.4%) might complicate with hepatitis B virus infection, whereas 12.0% of the students responded that bronchitis was one of the complications of hepatitis B infection. In summary of the above results, we highly recommend that health education program for eradication of hepatitis B virus infection should be introduced in curricula of secondary school education in this country.

• The Korean Journal of Mycology
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• v.16 no.1
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• pp.26-32
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• 1988

A total of 1,192 patients, who complained a continued chronic cough, suptum or occasional hemoptysis, in spite of successful completion of antituberculous chemotherapy or had some suspected fungal infection, were included. Serum specimens were collected from all the patients studied and sputum or other specimens collected and cultured from the most of the patients. 405(34.0%) cases of the total patients studied showed a positive precipitin reaction to the one or more of the fungal antigens on immunodiffusion tests and 303 cases of them were found to have been infected with Aspergilli, of which Aspergillus fumigatus was involved in 287 cases, followed by Aspergillus flavus(1.7%), Aspergillus nidulans(0.3%), Aspergillus niger(0.3%) and Aspergillus nidulans var. latus(0.1%). pricipitin antibodies were produced to Candida al­bicans(8.1%) and Pseudallerscheria boydii(0.8%). In the chest radiographs of 186 precipitin positive patients, distinct fungus ball shadows were seen in 47 cases and 45 cases of them were formed by A. fumigatus. The isolates from sputum specimens of 724 patients were aspergilli which were consisted of the 46.4% of the total fungal isolates. Identification of 137 yeast like fungi from the sputum specimens of 413 patients revealed that C. albicans(64.2%) was a commonest yeast flora.

• Journal of Environmental Health Sciences
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• v.39 no.5
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• pp.456-464
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• 2013

Objectives: The aim of this study was to investigate the microbial contamination levels in elevators in apartment buildings and to provide information on such microbial contamination. Methods: A total of 144 samples, including from the exterior buttons, interior buttons, elevator handrails, walls, ventilators and airborne bacteria were collected in the morning and afternoon from July to August 2013 for six different elevators. The samples were used to detect sanitary indicator bacteria (total bacteria, coliform, and Escherichia coli), pathogenic bacteria (E. coli O157, L. monocytogenes, Salmonella spp., B. cereus, S. aureus) and fungi. Results: Contamination levels of total bacteria were 0.3-3.8 and 0.0-2.4 log CFU/100 $cm^2$ in the morning and afternoon, respectively. In the case of coliform bacteria, the levels were 0.0-3.7 log CFU/100 $cm^2$ in the morning and 0.0-0.3 log CFU/100 $cm^2$ in the afternoon. However, E. coli was not detected among all samples. Bacillus cereus, pathogenic bacteria, was only detected in 13 (11%) among 144 samples. E. coli O157, L. monocytogenes, Salmonella spp. and S. aureus were not detected among all samples. Comparing the samples collected in the morning and afternoon, we could confirm that the samples in the afternoon were cleaner. Conclusions: This study indicates that the samples in the afternoon were cleaner because these samples were collected following routine cleaning. Also, the levels of contamination in the elevators were low and the sanitary conditions were comparatively well-managed. Therefore it is deemed necessary for elevators be cleaned regularly to provide good conditions for people using elevators.

• Journal of the Korea Organic Resources Recycling Association
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• v.24 no.4
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• pp.85-94
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• 2016

The purpose of this study was to characterize the coffee ground and its possibility to develop the antifungal activity. pH, EC, and Zn contents of the coffee ground from coffee shops were higher than those of commercial coffee ground, but there was no significant difference in $K_2O$, CaO, MgO, $Na_2O$, Mn contents. The antimicrobial activity of the water soluble extracts from the coffee shop and the commercial coffee ground were tested for six major plant pathogens, Rhizoctonia solani, Sclerotinia sclerotiorum, Fusarium oxysporum, Phytophthora capsici, Alternaria alternata, and Botrytis cinerea. The result showed that there was reliable antifungal activity against all of tested plant pathogenic fungi. The inhibition effects of coffee ground compost extract on the spore germination and zoospore formation were investigated. Water soluble extracts of the coffee ground compost mixture added with 10% sesame oil cake were significantly inhibited the growth of conidia germination of A. altanata and zoospore formation of P. capsici in vitro. For investigating the functional materials of coffee ground compost, it was measured the total polyphenolic compounds contents with 30 days interval during decomposing coffee ground for 90 days. The total polyphenolic content increased with decomposing periods, and it observed that the highest total polyphenolic content was $0.35{\pm}0.03mg\;GAE/g$ on the 90th day in the coffee ground compost added with 10% sesame oil cake.

• Journal of Plant Biology
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• v.13 no.1
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• pp.13-24
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• 1970

This investigation was one of the series of ecological studies on the plant communities of Quelpart Island which was held in 1969. It was aimed at studying the ground vegetation of Torreya nucifera and the soil properties of the ground in which it grows. Observaton of the flora in a place where wild growth of Crinum maritimum, soil properties and distribution of micro-organisms in a place where wild growth occurs, were also made. 1) A few kinds which have important values among the ground vegetation fo Torreya nucifera forest are Liniope graminifolia, Convallaria keiskei, Sasaquelpaertensis, Carex kingiana, Pteridium aquilinum, Pyrola japonica, Hedera tobleri, etc. 2) While the studies of 25 Quadrats were being held, 29 species in A plot, 30 species in B plot, and 26 species in C plot have been found. 3) The kind which has the highest important value among the species located in naked plot(outside of Torreya nucifera forest) is Zoysia japonica and 18 other kinds were found. 4) The soil fertility through all depths are found higher in the forest soil than in that of open places. 5) P2O5, K, and total exchangeable bases etc. have shown the difference of contents according to the depth and generally had lower value as much as it goes down. 6) The content of N was a lot higher than any other forest soil and P2O5, K and total exchangeable bases seemed to be a little lower. 7) The overall flora of Todo consists of 71 species (9 species of wood plant and 62 species of herbs) added 28 kinds of non-reported species. 8) Themineral contents are the highest at a place of wind growth of Crinum maritimum which has much organic matter. The subsoil of this place is more fertile than surface soil. 9) The soil of Todo which is sandy soil, consists of shellfish shell and quartz, and has low fertility and alkali. 10) In the total occurrences of micro-organisms, bacteria was the greatest in number followed by actinomycete and fungi, in that order. 11) A survey of the soil depth and the ecological distributional pattern of micro-organismsm revealed that the number of micro-organisms is the greatest on the surface, gradually decreasing in proportion to the depth. 12) It was found that a comparatively greater number of micro-organisms occurred when the soil showed an adequate increase in water content.

• Journal of Animal Science and Technology
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• v.48 no.5
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• pp.683-690
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• 2006

Two experiments were conducted to investigate the effects of molasses supplementation levels to silage materials in ensiling herbal medicine meal on the quality of silage, and its palatability in Korean native goats. In experimentⅠ, molasses were added at the levels of 0, 0.5, and 1.0% to herbal medicine (Deasihotang) meal, with three replicates per each level. They were kept at room temperature for 40 d in glass bottles in order to get silage samples. In experiment Ⅱ, nine 1.5-yr-old Korean native female goats were employed to measure their palatability. In experimentⅠ, lactic acid contents in molasses treatments were significantly (p<0.05) higher, and pH and butyric acid contents was lower than those of non-molasses control treatment. In addition, molasses treatments increased total microbial cell counts in MRS medium for lactobacillus, but decreased total microbial cell counts in PDA medium for fungi. Molasses supplementation to silage materials increased in vitro dry matter disappearance rate. Molasses supplementation tended to increase silage intake in Korean native goat (experiment II), however, the difference was not significant. It is concluded that molasses supplementation to silage materials in ensiling Daesihotang meal could improve its preservability and palatability, the larger the amount the better the quality.

• Korean Journal of Soil Science and Fertilizer
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• v.34 no.1
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• pp.8-16
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• 2001

This experiment was conducted to investigate the effects on plant growth, soil chemical properties and microbial flora with microbial fertilizer to chinese cabbage cultivation. The plant growth was promoted and the yield increased by application of Tian Li Bao(TLB) microbial fertilizer as compared with the control. However, yield a littler decreased in case of the reduced amount of urea application as a top dressing and half of compost chicken manure as a basial fertilizer even if treated with TLB microbial fertilizer. Organic matter and total nitrogen contents decreased as compared to those in the control, and total nitrogen ranged in 0.76~1.44% in the treatments at harvesting time, and decreased with application of TLB microbial fertilizer compared to that of the control. The available phosphorus content in the field before experiment was 559ppm, but it was 755ppm and 653 in the control and treatments at harvesting time, respectively. Therefore, it was shown that phosphorus content in the treatment was lower than that of the control. On the other hand, total nitrogen, phosphorous and K ranged from 2.62 to 2.94%, from 1.48 to 1.55% and from 3.60 to 4.38% in plants after harvest, respectively. There were no significant differences among the treatments. For the soil microbial flora, the population of bacteria in the treatments decreased with application of microbial fertilizer as compared with the control over all cultivation periods. It was shown that the population of pseudomonas spp. was over 3 times higher than that of the control after harvesting. The population of actinomycetes didn't show difference among the treatments, but high density of fungi after harvesting were observed in the treatments.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.6
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• pp.808-814
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• 2019

Objective: The objective of this study was to investigate the effects of essential oil mixture (EOM) supplementation on rumen fermentation characteristics and microbial changes in an in vitro. Methods: Three experimental treatments were used: control (CON, no additive), EOM 0.1 (supplementation of 1 g EOM/kg of substrate), and EOM 0.2 (supplementation of 2 g EOM/kg of substrate). An in vitro fermentation experiment was carried out using strained rumen fluid for 12 and 24 h incubation periods. At each time point, in vitro dry matter digestibility (IVDMD), neutral detergent fiber digestibility (IVNDFD), pH, ammonia nitrogen ($NH_3-N$), and volatile fatty acid (VFA) concentrations, and relative microbial diversity were estimated. Results: After 24 h incubation, treatments involving EOM supplementation led to significantly higher IVDMD (treatments and quadratic effect; p = 0.019 and 0.008) and IVNDFD (linear effect; p = 0.068) than did the CON treatment. The EOM 0.2 supplementation group had the highest $NH_3-N$ concentration (treatments; p = 0.032). Both EOM supplementations did not affect total VFA concentration and the proportion of individual VFAs; however, total VFA tended to increase in EOM supplementation groups, after 12 h incubation (linear; p = 0.071). Relative protozoa abundance significantly increased following EOM supplementation (treatments, p<0.001). Selenomonas ruminantium and Ruminococcus albus (treatments; p<0.001 and p = 0.005), abundance was higher in the EOM 0.1 treatment group than in CON. The abundance of Butyrivibrio fibrisolvens, fungi and Ruminococcus flavefaciens (treatments; p<0.001, p<0.001, and p = 0.005) was higher following EOM 0.2 treatment. Conclusion: The addition of newly developed EOM increased IVDMD, IVNDFD, and tended to increase total VFA indicating that it may be used as a feed additive to improve rumen fermentation by modulating rumen microbial communities. Further studies would be required to investigate the detailed metabolic mechanism underlying the effects of EOM supplementation.