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http://dx.doi.org/10.5713/ajas.18.0652

Essential oil mixture on rumen fermentation and microbial community - an in vitro study  

Kim, Hanbeen (Department of Animal Science, Life and Industry Convergence Research Institute, Pusan National University)
Jung, Eunsang (Department of Bioenvironmental Energy, Life and Industry Convergence Research Institute, Pusan National University)
Lee, Hyo Gun (Department of Animal Science, Life and Industry Convergence Research Institute, Pusan National University)
Kim, Byeongwoo (Department of Animal Science, Life and Industry Convergence Research Institute, Pusan National University)
Cho, Seongkeun (Department of Animal Science, Life and Industry Convergence Research Institute, Pusan National University)
Lee, Seyoung (Division of Animal Husbandry, Yonam College)
Kwon, Inhyuk (EASY BIO, Inc.)
Seo, Jakyeom (Department of Animal Science, Life and Industry Convergence Research Institute, Pusan National University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.32, no.6, 2019 , pp. 808-814 More about this Journal
Abstract
Objective: The objective of this study was to investigate the effects of essential oil mixture (EOM) supplementation on rumen fermentation characteristics and microbial changes in an in vitro. Methods: Three experimental treatments were used: control (CON, no additive), EOM 0.1 (supplementation of 1 g EOM/kg of substrate), and EOM 0.2 (supplementation of 2 g EOM/kg of substrate). An in vitro fermentation experiment was carried out using strained rumen fluid for 12 and 24 h incubation periods. At each time point, in vitro dry matter digestibility (IVDMD), neutral detergent fiber digestibility (IVNDFD), pH, ammonia nitrogen ($NH_3-N$), and volatile fatty acid (VFA) concentrations, and relative microbial diversity were estimated. Results: After 24 h incubation, treatments involving EOM supplementation led to significantly higher IVDMD (treatments and quadratic effect; p = 0.019 and 0.008) and IVNDFD (linear effect; p = 0.068) than did the CON treatment. The EOM 0.2 supplementation group had the highest $NH_3-N$ concentration (treatments; p = 0.032). Both EOM supplementations did not affect total VFA concentration and the proportion of individual VFAs; however, total VFA tended to increase in EOM supplementation groups, after 12 h incubation (linear; p = 0.071). Relative protozoa abundance significantly increased following EOM supplementation (treatments, p<0.001). Selenomonas ruminantium and Ruminococcus albus (treatments; p<0.001 and p = 0.005), abundance was higher in the EOM 0.1 treatment group than in CON. The abundance of Butyrivibrio fibrisolvens, fungi and Ruminococcus flavefaciens (treatments; p<0.001, p<0.001, and p = 0.005) was higher following EOM 0.2 treatment. Conclusion: The addition of newly developed EOM increased IVDMD, IVNDFD, and tended to increase total VFA indicating that it may be used as a feed additive to improve rumen fermentation by modulating rumen microbial communities. Further studies would be required to investigate the detailed metabolic mechanism underlying the effects of EOM supplementation.
Keywords
Feed Additive; In vitro Degradability; Microbial Abundance;
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Times Cited By KSCI : 2  (Citation Analysis)
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