• 한국토양비료학회지
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• 제43권6호
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• pp.1002-1007
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• 2010

본 연구는 균근균 접종이 포도삽목묘의 생장에 미치는 영향과 균근 형성율 그리고 형성 양상을 구명하고자 수행하였다. 포도 삽목시, 균근균 접종원을 처리한 거봉과 탐나라 품종은 무 접종처리 보다 엽수, 엽면적, 총 뿌리길이 그리고 뿌리 표면적이 크게 증가하였다. 그러나 켐벨어리 품종의 총 뿌리길이와 뿌리 표면적은 무처리구 (AMF-)와 비교하여 유의적인 차이가 없었다. 접종 8주후 균근균 접종 처리구 (AMF+)의 형성율은 22.5-32.5%이었으며, 12주 후 켐벨어리, 탐나라 그리고 거봉의 균근 형성율은 각각 29.6%, 28.8% 그리고 48.8%이었다. 반면 균근균 무처리구 (AMF-)는 균근형성 수준이 매우 낮았다.

• 한국콘텐츠학회:학술대회논문집
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• 한국콘텐츠학회 2017년도 춘계 종합학술대회 논문집
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• pp.105-106
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• 2017

Background: Atopic dermatitis one of the most common chronic skin diseases, is caused by various environmental and genetic factors. Methods: A total of 2609 healthy newborns who were enrolled in the COCOA study (COCOA) from 2008 to 2015 were surveyed for indoor environmental exposure to fungi during gestation and then diagnosed postnatally for atopic dermatitis. The fungi collected during the gestation of 20 normal subjects and 20 infants that developed atopic dermatitis were identified using Illumina's MiSeq platform and analyzed for their diversity and species. Results: A total of 2,609 respondents were surveyed (52.8% male and 47.2% female) Children, 1, 2, and 3 years old diagnosed with atopic dermatitis comprised 15.2%, 15.7%, and 14.1% of the respondents, respectively. The prevalence of exposure to mold during gestation was 1.46 (95% CI, 1.05-2.04) and 1.52 (95% CI, 0.95-2.43), in the first and third years after birth, respectively. One-year-old children with atopic dermatitis and no fungal markers detected in the bathroom environment during gestation accounted for less than 5% (aOR, 1.51; 95%CI, 0.96-2.38) and in the group less than 5 ~ 30% (aOR, 2.21; 95%CI, 1.00-4.89), 3-year-old children had an increased prevalence of atopic dermatitis of more than 30% (aOR, 9.48, 95%CI 1.42-63.13). Conclusions: Exposure to indoor fungi during gestation and infancy is associated with the development of atopic dermatitis in children. The phyla and genera of the fungi in the indoor house dust differed during gestation. This suggests that exposure to indoor fungi during gestation may be associated with the development of atopic dermatitis in children. Future research will be necessary to establish the underlying mechanisms.

• Journal of Microbiology and Biotechnology
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• 제23권1호
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• pp.40-46
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• 2013

A variety of nuruk were collected from various provinces in Korea, and their microflora profiles were analyzed at the species level. A total of 42 nuruk samples were collected and when the viable cell numbers in these nuruk were enumerated, the average cell numbers of bacteria, fungi, yeast, and lactic acid bacteria from all nuruk were 7.21, 7.91, 3.49, and 4.88 log CFU/10 g, respectively. There were no significant differences in viable cell numbers of bacteria or fungi according to regions collected. Bacillus amyloliquefaciens and B. subtilis were the predominant bacterial strains in most samples. A significant portion, 13 out of 42 nuruk, contained foodborne pathogens such as B. cereus or Cronobacter sakazakii. There were various species of lactic acid bacteria such as Enterococcus faecium and Pediococcus pentosaceus in nuruk. It was unexpectedly found that only 13 among the 42 nuruk samples contained Aspergillus oryzae, the representative saccharifying fungi in makgeolli, whereas a fungi Lichtheimia corymbifera was widely distributed in nuruk. It was also found that Pichia jadinii was the predominant yeast strain in most nuruk, but the representative alcohol fermentation strain, Saccharomyces cerevisiae, was isolated from only 18 out of the 42 nuruk. These results suggested that a variety of species of fungi and yeast were distributed in nuruk and involved in the fermentation of makgeolli. In this study, a total of 64 bacterial species, 39 fugal species, and 15 yeast species were identified from nuruk. Among these strains, 37 bacterial species, 20 fungal species, and 8 yeast species were distributed less than 0.1%.

• Mycobiology
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• 제49권2호
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• pp.151-160
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• 2021

Despite recent studies, relatively few are known about the diversity of fungal communities in the deep Atlantic Ocean. In this study, we investigated the diversity of fungal communities in 15 different deep-sea sediments from the South Atlantic Ocean with a culture-dependent approach followed by phylogenetic analysis of ITS sequences. A total of 29 fungal strains were isolated from the 15 deep-sea sediments. These strains belong to four fungal genera, including Aspergillus, Cladosporium, Penicillium, and Alternaria. Penicillium, accounting for 44.8% of the total fungal isolates, was a dominant genus. The antiaflatoxigenic activity of these deep-sea fungal isolates was studied. Surprisingly, most of the strains showed moderate to strong antiaflatoxigenic activity. Four isolates, belonging to species of Penicillium polonicum, Penicillium chrysogenum, Aspergillus versicolor, and Cladosporium cladosporioides, could completely inhibit not only the mycelial growth of Aspergillus parasiticus mutant strain NFRI-95, but also the aflatoxin production. To our knowledge, this is the first report to investigate the antiaflatoxigenic activity of culturable deep-sea fungi. Our results provide new insights into the community composition of fungi in the deep South Atlantic Ocean. The high proportion of strains that displayed antiaflatoxigenic activity demonstrates that deep-sea fungi from the Atlantic Ocean are valuable resources for mining bioactive compounds.

• 한국식품과학회지
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• 제23권5호
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• pp.572-577
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• 1991

국내에서 재래적인 방법에 의하여, 생산 시판되고 있는 전통 발효식품인 메주(12종), 된장(28종) 및 간장(28종)을 수거하여 이들로부터 OA를 생성하는 fungi를 분리 및 동정하였고, 동정된 fungi 중 OA를 가장 많이 생성하는 4 isolates을 선택하여 이들의 생육과 OA 생성 특성을 조사하였다. 각 시료에서 불리해낸 222 fungi 중 OA를 생성하는 것은 39 isolates로, 곰팡이의 OA 생성률이 17.7%(39/222)이며, 이들 중 가장 많은 OA를 생성하는 4 Fungi는 Penicillium spp., Phialotubus microsporus, Eupenicillium lapidosum 및 Paecilomyces variotti이었다. 이들 균주는 $a_{w}$ 0.99에서 가장 잘 자랐고 $a_{w}$ 0.85에서 OA의 생성이 중지되었다. 또한 $30^{\circ}C$에서 억제되었다. Toxin 생성 최적 pH는 Paecilomyces variotti을 제외하고는 6.5이었으며 pH에 의해서 toxin 생성은 $a_{w}$와 달리 영향을 크게 받지 않았다.

• Journal of Forest and Environmental Science
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• 제34권2호
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• pp.126-135
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• 2018

Wood decay fungi were collected in 47 different locations in eight provinces of South Korea from 2011 to 2013. One thousand and five hundreds three fruiting bodies of wood-decay fungi were collected, identified, and classified into 2 phyla, 7 classes, 19 orders, 56 families, 159 genera and 365 species. The most dominant genus and species found were Trametes and T. versicolor. The highest species diversity was found in broad-leaved forest (273 species), and was also found at elevations of 500-1,000 m (227 species). A total of 333 species were collected from broad-leaved trees, 87 species from coniferous trees, and 55 species were collected from both forest types. Gymnopilus liquiritiae was the most dominant species in coniferous trees, while T. versicolor, which was mostly collected from tree trunks below 500 m in elevation, was dominant in broad-leaved trees. Results from the quantitative cluster analysis of wood decay fungi showed that the highest species diversity index was 1.80 in the mixed forests, while the highest similarity among forest types was shown between the broad-leaved and mixed forests.

• 한국균학회지
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• 제40권4호
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• pp.183-186
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• 2012

곰솔(Pinus thunbergii Parl.)의 잎에 서식하는 내생균의 다양성을 확인하기 위하여 충남 보령의 해안가에 서식하는 곰솔에서 잎을 채취하여 총 19개의 균주를 분리하였다. 분리된 내생균을 ITS 지역과 LSU 지역의 염기서열을 분석하여 동정한 결과 Anthostomella sepelibilis, Beltrania sp., Cladosporium sp., Coniochaeta velutina, Entonaema pallida, Lophodermium sp., Nigrospora sphaerica 등 총 7개 분류군의 내생균을 확보할 수 있었다. 이들 중 Beltrania sp.에 속하는 종이 가장 높은 빈도로 분리되었으며 내생균의 서식 환경이 곰솔의 잎에 서식하는 내생균의 분포에 영향을 미칠 수 있음을 확인하였다.

• The Plant Pathology Journal
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• 제23권4호
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• pp.287-294
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• 2007

Endophytic fungi were isolated from surface sterilized root tissues of Aralia elata and Aralia continentalis, collected from farmer's field in Chungnam province, Republic of Korea, in 2005. Based on ITS sequence analysis, 24 fungal genera were characterized from 359 isolates, belonging to 22 Ascomycota, 1 Glomeromycota and 1 Oomycota. Strumella, Rhizopycnis and Entrophospora in A. elata and Rhizopycnis and Leptosphaeria in A. continentalis were the most abundant taxa. Out of 24 genera, Entrophospora, Leptodontidium, Neoplaconema, Paraconiothyrium, Rhizopycnis, Strumella and Tumularia were new to Korea. A total of 110 isolates were tested for antifungal activities against six plant pathogenic fungi. Out of these, 39 isolates showed antifungal activity against at least one plant pathogenic fungi. Four isolates of Pyrenochaeta, 1 isolate of Entrophospora and 1 unidentified fungus strongly inhibited the growth of six plant pathogenic fungi.

• 한국응용곤충학회지
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• 제36권1호
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• pp.37-42
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• 1997

우리나라의 곤충병원성곰팡이 분포를 조사하기 위하여 5개도와 3개 광역시에서 200개소의 토양 sample을 채집하여 꿀벌부채명나방 유충을 이용하여 분리하였다. 8종의 곤충 사체로 부터는 직접 분리하였다. 그 결과 40개소(20.0%)의 토양과 8종의 곤충 사체에서 곰팡이가 분리되었는데, 토양에서 분리된 균종별로는 Bequveria가 21개소(10.5%), Metarhizium이 19개소(9.5%)이었다. 그외 7종의 곤충에서 Beauvria가 분리되었다. 서식처별로는 산림 110개소중 25개소(22.7%), 경작지 20개소중 2개소(11.0%), 휴한지 11개소중 6개소(54.5%), 하천 제방 19개소중 7개소(36.8%)에서 곤충병원성곰팡이가 검출되었다. 한편, 곤충 사체에서는 긴수염 대벌레에서 Metarhizium이 분리된 것을 제외하고는 모두 Beauveria가 분리되었다.

• Mycobiology
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• 제50권5호
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• pp.357-365
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• 2022

Schizophyllum commune has emerged as the most promising model mushroom to study developmental stages (mycelium, primordium), which are two primary processes of fruit body development. Long non-coding RNA (lncRNA) has been proved to participate in fruit development and sex differentiation in fungi. However, potential lncRNAs have not been identified in S. commune from mycelium to primordium developmental stages. In this study, lncRNA-seq was performed in S. commune and 61.56 Gb clean data were generated from mycelium and primordium developmental stages. Furthermore, 191 lncRNAs had been obtained and a total of 49 lncRNAs were classified as differently expressed lncRNAs. Additionally, 26 up-regulated differently expressed lncRNAs and 23 down-regulated between mycelium and primordia libraries were detected. Further, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that differentially expressed lncRNAs target genes from the MAPK pathway, phosphatidylinositol signal, ubiquitin-mediated proteolysis, autophagy, and cell cycle. This study provides a new resource for further research on the relationship between lncRNA and two developmental stages (mycelium, primordium) in S. commune.