Browse > Article
http://dx.doi.org/10.5423/PPJ.2007.23.4.287

Fungal Endophytes in Roots of Aralia Species and Their Antifungal Activity  

Paul, Narayan Chandra (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
Kim, Won-Ki (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
Woo, Sung-Kyoon (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
Park, Myung-Soo (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
Yu, Seung-Hun (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
Publication Information
The Plant Pathology Journal / v.23, no.4, 2007 , pp. 287-294 More about this Journal
Abstract
Endophytic fungi were isolated from surface sterilized root tissues of Aralia elata and Aralia continentalis, collected from farmer's field in Chungnam province, Republic of Korea, in 2005. Based on ITS sequence analysis, 24 fungal genera were characterized from 359 isolates, belonging to 22 Ascomycota, 1 Glomeromycota and 1 Oomycota. Strumella, Rhizopycnis and Entrophospora in A. elata and Rhizopycnis and Leptosphaeria in A. continentalis were the most abundant taxa. Out of 24 genera, Entrophospora, Leptodontidium, Neoplaconema, Paraconiothyrium, Rhizopycnis, Strumella and Tumularia were new to Korea. A total of 110 isolates were tested for antifungal activities against six plant pathogenic fungi. Out of these, 39 isolates showed antifungal activity against at least one plant pathogenic fungi. Four isolates of Pyrenochaeta, 1 isolate of Entrophospora and 1 unidentified fungus strongly inhibited the growth of six plant pathogenic fungi.
Keywords
antifungal activity; Aralia continentalis; Aralia elata; endophytic fungi; ITS sequence;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 6  (Related Records In Web of Science)
연도 인용수 순위
1 Bussaban, B., Lumyong, S., McKenzie, E. H. C. and Hyde, K. D. 2001. Endophytic fungi from Ammonium siamense. Can. J Microbiol. 47:943-948
2 Carter, J. P., Spink, J., Cannon, P. F., Daniels, M. J. and Osbourn, A. E. 1999. Isolation, characterization, and sensitivity of a diverse collection of cereal-root-colonizing fungi. Appl. Environ. Microbiol. 3364-3372
3 Fisher, P. J., Petrini, O. and Lappin, S. H. M. 1992. The distribution of some fungal and bacterial endophytes in maize (Zea mays L.). New Phytol. 122:299-305   DOI   ScienceOn
4 Huang, Y., Wang, J., Li, G., Zheng, G. and Su, W. 2001. Antitumour and antifungal activities in endophytic fungi isolated from pharmaceutical plants Taxus mairei, Cephalataxus fortunei and Torreya grandis. FEMS Immunol. Medical Microbiol. 31:163-167
5 Larran, S., Monaco, C. and Alippi, H. 2000. Endophytic fungi in beet (Beta vulgaris var. esculenta L) leaves. Adv. Hort. Sci. 14:193-196
6 Paul, N. C., Kim, W. K., Woo, S. K., Park, M. S. and Yu, S. H. 2006. Diversity of endophytic fungi associated with Taraxacum coreanum and their antifungal activity. Mycobiol. 34:185-190   과학기술학회마을   DOI   ScienceOn
7 Petrini, O. 1991. Fungal endophytes of tree leaves. In: Microbial ecology of leaves, ed. by J. H. Andrewsand S. S. Hirano, Springer-Verlag, New York. USA
8 Schardl, C. L., Liu, J., White, J. K., Finkel, R. A., An, Z. and Siegel, M. 1991. Molecular phylogenetic relationship of nonpathogenic grass mycosymbionts and clavicipitaceous plant pathogens. Plant Syst. Evol. 178:27-41   DOI
9 Sieber, T., Riesen, T. K. Muller, E. and Fried, P. M. 1988. Endophytic fungi in four winter cultivars (Triticum aestivum L.) differing in resistance against Stagonospora nodorum (Berk.) Cast. And germ.= Septoria nodorum (Berk.) Berk. J. Phytopathol. 122:2289-2306
10 Stierle, A., Strobel, G. and Stierle, D. 1993. Taxol and taxane production by Taxomyces andreanae and endophytic fungus of Pacific Yew. Science 260:214-216   DOI
11 Taechowisan, T., Peberdy, J. F. and Lumyong, S. 2003. Isolation of endophytic actinomycetes from selected plants and their antifungal activity. World J. Microbiol. Biotech. 19:381-385   DOI   ScienceOn
12 The Korean Society of Plant Pathology. 2004. List of Plant Diseases in Korea. 4th ed. The Korean Society of Plant Pathology, Korea
13 Clay, K. 1989. Clavicipitaceous endophytes of grasses: their potential as biocontrol agents. Mycol. Res. 92: 1-12   DOI
14 Tian, X. L., Cao, L. X., Tan, H. M., Zeng, Q. G., Jia, Y. Y., Han, W. Q. and Zhou, S. N. 2004. Study on the communities of endophytic fungi and endophytic actinomycetes from rice and their antipathogenic activities in vitro. World J. Microbiol. Biotech. 20:303-309   DOI
15 Fisher, P. J., Petrini, O. and Petrini, L. E. 1991. Endophytic ascomycetes and deuterornycetes in roots of Pinus sylvestris. Nova Hedwigia. 52: 11-15
16 Tejesvi, M., Nahesh, B., Nalini, M., Prakash, H., Kini, K., Subbiah, V. and Shetty, H. 2005. Endophytic fungal asemblanges from inner bark and twig of Terminalia arjuna W & A (Combretaceae). World J. Michobiol Biotech. 21: 1535-1540   DOI
17 Li, H., Qing, C., Zhang, Y. and Zhao, Z. 2005. Screening for endophytic fungi with antitumour and antifungal activities from Chinese medicinal plants. World J. Microbiol. Biotech. 21:1515-1519   DOI
18 Petrini, O. and Carroll, G.C. 1981. Endophytic fungi in foliage on some Cupressaceae in Oregon. Can. J. Bot. 59:629-636   DOI
19 Rubini, M. R., Silva-Ribeiro, R. T., Pomella, A. W. V., Maki, C. S., Araujo, W. L., Santos, D. R. and Azevedo, J. L. 2005. Diversity of endophytic fungal community of cacao (Theobroma cacao L.) and biological control of Crinipellis pemiciosa, causal agent of witches broom disease. Int'l. J. Biol. Sci. 1:24-33
20 Holderiender, O. and Sieber, T. N. 1992. Fungal associations of serially washed healthy non-mycorrhizal roots of Picea abies. Mycol. Res. 96:151-156   DOI
21 Strobel, G. A., Hess, W. M., Ford, E., Sidhu, R. S. and Yang, X. 1996. Taxol from fungal endophytes and the tissue of biodiversity. J. Ind. Microbiol. 17:417-423   DOI
22 Strobel, G. and Daisy, B. 2003. Bioprocessing for microbial endophytes and their natural products. Microbiol. Mol. Biol. Rev. 67:491-502   DOI
23 Dorworth, C. E. and Callan, B. E. 1996. Manipulation of endophytic fungi to promote their utility as vegetation biocontrol agents. In: Endophytic fungi in grasses and woody plants, ed, by S. C. Reddin and L. M. Carris. APS Press, St. Paul, MN, USA
24 Fisher, P. J., Andoson, A. E. and Petrini, O. 1984. Antibotic activity of some endophytic fungi from ericaceous plants. Botanica Helvetica. 94:249-253
25 Carroll, G C. 1991. Fungal associations of woody plants as insect antagonists in leaves and stems. In: Microbial mediation of plant-herbivore interactions, ed. by P. Barbosa, V. A. Krischick and C. G. Jones, Wiley, New York. USA
26 Bills, G. F. 1996. Isolation and analysis of endophytic fungal communities from woody plants. In: Systematics, ecology and evolution of endophytic fungi in grasses and woody plants, ed. by Redlin, S. and Carris, L. M. pp. 31-65. APS press. St Paul, MN, USA
27 Carroll, G. C. 1988. Fungal endophytes in stems and leaves: from latent pathogens to mutualistic symbiont. Ecology 69:2-9   DOI   ScienceOn
28 Stefan, G. R., Leibinger, W., Ernst, M. and Mendgen, K. 2001. Genetic diversity of fungi closely associated with common reed. New Phytol. 149:589-598   DOI
29 Carroll, G. C. 1986. The biology of endophytism in plants with particular reference to woody perennials. In: Microbiology of the Phyllosphaere, ed. by N. J. Fokkema and J. Van den Heuve. Cambridge University Press, Cambridge. Unite Kingdom
30 Dreyfuss, M. M. and Chapela, I. 1994. Potential of fungi in the discovery of novel, low-molecular weight pharmaceuticals. In: The discovery of natural products with therapeutic potential, ed. by V. P. Gullo, pp. 49-80. Butter-Worth-Heinemann, London, United Kingdom
31 Phongpaichit, S., Rungjindama, N., Rukachaisirikul, V. and Sakayaroj, J. 2006. Antimicrobial activity in cultures of endophytic fungi isolated from Garcinia species. FEMS Immunol. Med. Microbiol. 48:367-372   DOI   ScienceOn
32 Mcinroy, J. A. and Kloepper, J. W. 1991. Endophytic bacteria from field-grown com and cotton. Phytopathology 81:812-813
33 Bacon, C. W. 1990. Isolation, culture and maintenance of endophytic fungi of grasses In: Isolation of biotechnological organisms from nature, ed. by D. P. Labeda, McGraw-Hill, New York, USA
34 Larran, S., Perello, A, Simon, M. R. and Moreno, V. 2002. Isolation and analysis of endophytic microorganisms in wheat (Triticum aestivum L.) leaves. World J. Microbiol. Biotech. 18:683-686   DOI   ScienceOn
35 Fisher, P. J., Andoson, A. E. and Petrini, O. 1986. Fungal endophytes in Ulex europaeus and Ulex gallii. Trans Br. Mycol. Soc. 86: 153-156   DOI
36 Lu, H., Zou, W. X., Meng, J. C., Hu, J. and Tan, R. X. 2000. New bioactive metabolites produced by Colletotrihum sp., an endophytic fungus in Artemisia annua. Plant Sci. 151:67-73   DOI   ScienceOn
37 Seena, S. and Sridhar, K. R. 2004. Endophytic fungal diversity of 2 sand dune wild legumes from the southwest coast of India. Can. J. Microbiol. 50:1015-1021   DOI   ScienceOn
38 Murray, F. R., Latch. G. C. M. and Scott, D. B. 1992. Surrogate transformation of perennial rye grass, Lolium perenne, using genetically modified Acremonium endophyte. Mol. Gen. Genet. 233:1-9   DOI
39 Naik, B. S., Shashikala, J. and Krishnomurthy, Y. L. 2007. Study on the diversity of endophytic communities from rice (Oryza sativa L.) and their antagonistic activities in vitro. Microbiol Res. 2007; doi: 10.1016/j.micres. 2006.12.003
40 Park, J. H., Park, J. H., Choi, G. J., Lee, S. W., Jang, K. S., Choi, Y. H., Cho, K. Y. and Kim, J. C. 2003. Screening for antifungal endophytic fungi against six plant pathogenic fungi. Mycobiol. 31:179-182   과학기술학회마을   DOI   ScienceOn
41 Spurr, H. W. Jr. and welty, R. E. 1975. Characterization of endophytic fungi in healthy leaves of Nicotiana spp. Phytopathology 65:417-422   DOI
42 Larran, S., Monaco. C. and Alippi, H. 2001. Endophytic fungi in leaves of Lycopersicon esculentum Mill. World J. Microbiol. Biotech. 17:181-184   DOI
43 Pereira, J. O., Carneiro, Vieira, M. L. and Azevedo, J. L. 1999. Endophytic fungi from Musa acuminata and their reintroduction into axenic plants. World J. Microbiol. Biotech. 15:37-40   DOI
44 Aghighi, S., Bonjar, G. H. S., Rawashdeh, R., Batayneh, S. and Saadoun, I. 2004. First report of antifungal spectra of activity of Iranian actinomycetes strains against Alearia solani, Alternaria alternata, Fusarium solani, Phytophthora megasperma, Verticillium dahliae and Saccharomyces cerevisiae. Asian J. Plant Sci. 3:463-471   DOI