• Clinical and Experimental Reproductive Medicine
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• 제40권1호
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• pp.23-28
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• 2013

Objective: The aim of the present study was to examine the relationship among male age, strict morphology, and sperm chromatin structure and condensation. Methods: Sperm samples from a total of 100 men underwent semen analysis, and sperm chromatin structure and condensation were assessed with toluidine blue (TB) and aniline blue (AB) tests. Results: Prevalence of strict morphology of less than 4%, and abnormal sperm chromatin structure and condensation did not show any statistically significant differences according to male age (p=0.605, p=0.235, and p=0.080). No significant correlation was demonstrated among age of male partners, strict morphology, and abnormal sperm chromatin structure using TB and AB tests. However, abnormal sperm chromatin condensation was positively associated with sperm chromatin structure (r=0.594, p=0.000) and showed negative correlation with strict morphology (r=-0.219, p=0.029). Conclusion: The tests for sperm chromatin condensation showed a significant association with strict morphology. Further study is needed to elucidate the relationship between clinical outcome and sperm chromatin tests.

• Asian Pacific Journal of Cancer Prevention
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• 제14권12호
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• pp.7345-7349
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• 2013

Background: Because of the high prevalence of hepatocellular carcinoma (HCC) in Egypt, new markers with better diagnostic performance than alpha-feto protein (AFP) are needed to help in early diagnosis. The aim of this work was to compare the clinical utility of both serum and mRNA glypican3 (GPC3) as probable diagnostic markers for HCC among Egyptian patients. Materials and Methods: A total of 60 subjects, including 40 with HCC, 10 with cirrhosis and 10 normal controls were analyzed for serum GPC3 (sGPC3) by ELISA. GPC-3 mRNA from circulating peripheral blood mononuclear cells was amplified by RT-PCR. Both markers were compared to some prognostic factors of HCC, and sensitivity of both techniques was compared. Results: Serum glypican-3 and AFP were significantly higher in the HCC group compared to cirrhotic and normal controls (p<0.001). Sensitivity and specificity were (95% each) for sGlypican-3, (82.5% and 85%) for AFP, and (100% and 90%) for Glypican3 mRNA, and (80% and 95%) for double combination between sGPC3 and AFP respectively. Conclusion: Both serum GPC-3 and GPC-3mRNA are promising diagnostic markers for early detection of HCC in Egyptian patients. RT- PCR proved to be more sensitive (100%) than ELISA (95%) in detecting glypican3.

• Journal of Korean Neurosurgical Society
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• 제58권2호
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• pp.93-100
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• 2015

Objective : Optimal treatment decision and estimation of the prognosis in traumatic brain injury (TBI) is currently based on demographic and clinical predictors. But sometimes, there are limitations in these factors. In this study, we analyzed three central nervous system biomarkers in TBI patients, will discuss the roles and clinical applications of biomarkers in TBI. Methods : From July on 2013 to August on 2014, a total of 45 patients were included. The serum was obtained at the time of hospital admission, and biomarkers were extracted with centrifugal process. It was analyzed for the level of S-100 beta (S100B), glial fibrillary acidic protein (GFAP), and ubiquitin carboxy-terminal hydrolase-L1 (UCH-L1). Results : This study included 33 males and 12 females with a mean age of 58.5 (19-84) years. TBI patients were classified into two groups. Group A was severe TBI with Glasgow Coma Scale (GCS) score 3-5 and Group B was mild TBI with GCS score 13-15. The median serum concentration of S100B, GFAP, and UCH-L1 in severe TBI were raised 5.1 fold, 5.5 fold, and 439.1 fold compared to mild injury, respectively. The serum levels of these markers correlated significantly with the injury severity and clinical outcome (p<0.001). Increased level of markers was strongly predicted poor outcomes. Conclusion : S100B, GFAP, and UCH-L1 serum level of were significantly increased in TBI according to severity and associated clinical outcomes. Biomarkers have potential utility as diagnostic, prognostic, and therapeutic adjuncts in the setting of TBI.

• The Korean Journal of Physiology and Pharmacology
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• 제18권6호
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• pp.447-456
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• 2014

Until now, a disease-modifying therapy (DMT) that has an ability to slow or arrest Alzheimer's disease (AD) progression has not been developed, and all clinical trials involving AD patients enrolled by clinical assessment alone also have not been successful. Given the growing consensus that the DMT is likely to require treatment initiation well before full-blown dementia emerges, the early detection of AD will provide opportunities to successfully identify new drugs that slow the course of AD pathology. Recent advances in early detection of AD and prediction of progression of the disease using various biomarkers, including cerebrospinal fluid (CSF) $A{\beta}_{1-42}$, total tau and p-tau181 levels, and imagining biomarkers, are now being actively integrated into the designs of AD clinical trials. In terms of therapeutic mechanisms, monitoring these markers may be helpful for go/no-go decision making as well as surrogate markers for disease severity or progression. Furthermore, CSF biomarkers can be used as a tool to enrich patients for clinical trials with prospect of increasing statistical power and reducing costs in drug development. However, the standardization of technical aspects of analysis of these biomarkers is an essential prerequisite to the clinical uses. To accomplish this, global efforts are underway to standardize CSF biomarker measurements and a quality control program supported by the Alzheimer's Association. The current review summarizes therapeutic targets of developing drugs in AD pathophysiology, and provides the most recent advances in the clinical utility of CSF biomarkers and the integration of CSF biomarkers in current clinical trials.

• 한국습지학회지
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• 제7권2호
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• pp.89-104
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• 2005

우리나라 서 남해안에 위치한 59개 갯벌의 다모류 출현 양상을 바탕으로 갯벌의 생태등급을 평가하였다. 갯벌의 생태등급 평가는 다모류의 분포밀도, 다양성, 풍부성, 자원적 가치, 종 위험도 등의 생물학적인 자료와 해안선의 자연성, 훼손 가능성, 지형경관 등의 인문지리학적인 면을 평가기준으로 하였다. 조사지역에서 출현한 다모류는 총 14목 37과 181종이었으며, 각 조사지역의 생태등급은 다음과 같다. I 등급 4개 지역(충남 학암포, 전남 우이도와 거문도, 경남 비진도와 매물도), II 등급 30개 지역, III 등급 19개 지역, IV 등급 4개 지역이었다. 이번 조사 결과 생태등급 기준에 대한 보다 면밀한 검토가 필요할 것으로 생각되며, 선정된 각 해역의 생태등급에 따른 정기적인 생물 조사 및 생태계 위협요인의 제거와 같은 실질적인 관리 방안을 마련하여야 할 것으로 판단된다. 특히 생물상 및 자연성이 높은 생태등급 지역의 경우 보다 적극적인 보전 노력이 요구된다.

• Asian Pacific Journal of Cancer Prevention
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• 제14권3호
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• pp.1819-1823
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• 2013

Objective: Microarray data were analyzed to explore key genes and their functions in progression of colorectal cancer (CRC). Methods: Two microarray data sets were downloaded from Gene Expression Omnibus (GEO) database and differentially expressed genes (DEGs) were identified using corresponding packages of R. Functional enrichment analysis was performed with DAVID tools to uncover their biological functions. Results: 631 and 590 DEGs were obtained from the two data sets, respectively. A total of 32 common DEGs were then screened out with the rank product method. The significantly enriched GO terms included inflammatory response, response to wounding and response to drugs. Two interleukin-related domains were revealed in the domain analysis. KEGG pathway enrichment analysis showed that the PPAR signaling pathway and the renin-angiotensin system were enriched in the DEGs. Conclusions: Our study to systemically characterize gene expression changes in CRC with microarray technology revealed changes in a range of key genes, pathways and function modules. Their utility in diagnosis and treatment now require exploration.

• 한국전자거래학회지
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• 제18권4호
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• pp.327-338
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• 2013

IT프로젝트를 수행하는 기업은 정해진 예산범위 내에서 투자계획을 수립하고 실행하게 된다. 이 과정에서 IT프로젝트를 효과적, 효율적으로 선정하는 문제는 기업경쟁력과 직결되는 중요한 요소이다. 그동안 IT프로젝트의 선정을 위한 다양한 모형이 개발되었으나 예산제약을 고려한 효과적 프로젝트 선정에 대한 연구는 보고되지 않고 있다. 본 연구에서는 예산 제약을 고려하여 효과적으로 IT프로젝트를 선정하는 기법을 제시하였다. 본 연구에서 제안한 모델(AHP-K)에서는 AHP(Analytic Hierarchy Process : 계층분석법) 기법을 적용하여 평가 항목의 가중치와 프로젝트 후보군의 가중치를 산정한 후 배낭문제(Knapsack Problem)를 적용하여 예산 범위 내에서 프로젝트 효용을 최대화하는 선정안을 도출한다. 실제 적용단계에서 고려하는 대안이 많거나 신규대안이 추가되는 경우 쌍대비교의 수행이 어려운 점을 감안하여 대안 비교 단계에서 절대평가법을 적용하였다. 본 연구에서는 제시된 모델을 실제 사례에 적용하여 기존 AHP 모형과 비교 분석함으로써 효과성을 검증하였다.

• 한국강구조학회 논문집
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• 제21권4호
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• pp.361-373
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• 2009

유전자 알고리즘을 이용한 닐센아치교의 최적설계기법을 이 논문에서 제시하였다. 설계 매개변수로는 닐센아치교의 아치-라이즈비와 강중비에 대해서 최적화기법을 적용하여 각각의 거동을 분석하고, 적정성을 평가하여 최적의 매개변수 값을 결정하였다. 매개변수의 결정은 구조물의 안전성과 사용성 그리고 경제성에 중요한 설계인자로서 정형화가 요구된다. 이를 위해 최적화 기법으로 전역 최적해 탐색능력이 우수한 유전자 알고리즘을 사용하였으며, 설계 목적함수로는 구조물의 총 중량을 사용하였고, 제약조건으로는 변위, 응력, 시공성 제약조건을 두었다. 구조해석은 미소변위이론에 의한 탄성해석을 수행하여 유전자 알고리즘과 조합하여 병렬연산으로 수행시간을 단축시켰다. 이 연구에서 개발된 최적설계기법을 사용하여 최적의 아치-라이즈비와 강중비, 최적설계영역을 제시 하였으며 실무에 적용할 수 있도록 하였다.

• 한국과학교육학회지
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• 제15권1호
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• pp.39-53
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• 1995

The purpose of this study was development of 'Environmental Science' of high school appropriate to Sixth Natinal Education Curriculum. In view that ultimate aim of environmental education is forming responsible environmental behaviors and the goals of values and behaviors are as important as knowledges and skills, A new environmental text of high school level was developed and it was based on analysis of seven texts and environmental education in Fifth Korean Curriculum. This text have seven units, 1.Habitates : What're the Meanings?, 2.Nuclear Energy: Can't be Avoid?, 3.Acid Rain : What're the Messages", 4.Ethanol : Is this Future Fuel?, 5.Wastes : A New War!, 6.What're the National and Global Environmental Issues? and 7.Our Water: Can Drink, Really? This text was stressed equally in goals of four environmental education and avoided from the array of knowledges. Therefore included various teaching strategies and independent actions of students. 'Open-ended value learning' and 'free behavior learning' in text were special learning parts for aquisition of values and formation of behaviors. To verify the effects of new developed environmental text, the direct learning was carried out by 286 students in total. Post test scores of experimental groups per each units were significantly higher than those of control groups about four goals, respectively. The Results of questionnaires by 50 teachers from five different schools were as follows. For validity of selecting contents for units, 74% of respondents replied positively. For classification and presentation of four goal-groups, 90% replied positively in validity and 82%, in utility. For validity of various teaching strategies, 88% and for the degree of including student-centered independent actions, 86% replied positively. For importances and expected effects of 'open-ended value learning' and 'free behavior learning', showed positive responses respectively, 88%, 92%. Therefore this text is effective to acheive four goals of environmental education equally.

• 한국융합학회논문지
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• 제6권6호
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• pp.75-80
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• 2015

ICT 기반의 융합전문가를 양성하기 위해서는 사물인터넷 활용 능력을 학습하여야 한다. 본 논문에서는 컴퓨터학을 전공한 교육생을 대상으로 5개월의 사물인터넷 관련 교육을 실시하여 융합전문가로 양성하기 위한 교육과정과 교육운영 전반을 다루는 교육모형 설계에 대한 내용을 기술한다. 교육과정은 기본역량과정, 핵심역량과정, 실전역량과정 그리고 현장연수과정으로 구분하여 총 10단계의 세부과정으로 구성하였다. 교과과정은 센싱기술, 네트워크기술, 보안 및 콘테츠 제작기술을 학습하도록 설계하였다. 본 교육모형은 2015년 고용노동부의 일자리창출 인력양성사업에 활용되어 높은 수료율과 취업률을 달성하는데 기여하여 효용성을 입증하였으며, 추후 대학교육에 적용할 수 있도록 Java 과정을 보완하는 등 교과과정을 확장할 계획이다.