• Asian-Australasian Journal of Animal Sciences
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• 제19권11호
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• pp.1546-1550
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• 2006

The purpose of this study was to assess the genetic variation and establish the relationship amongst breeds and strains using 15 chicken specific microsatellite markers. A total of 285 unrelated DNA samples from four Korean native chicken strains (Black strain of Korean native chicken; KL, Red Brown strain of Korean native chicken; KR, Ogol strain of Korean native chicken; KS and Yellow Brown strain of Korean native chicken; KY) and three introduced chicken breeds (F strain of White Leghorn; LF, K strain of White Leghorn; LK, Rhode Island Red; RC and Cornish; CN) were genotyped to estimate within and between breed genetic diversity indices. All the loci analyzed in 15 microsatellite markers showed a polymorphic pattern and the number of alleles ranged from 5 to 14. The polymorphism information content (PIC) of UMA1019 was the highest (0.872) and that of ADL0234 was the lowest (0.562). The expected total heterozygosity (He) within breed and mean number of observed alleles ranged from 0.540 (LF) to 0.689 (KY), and from 3.47 (LK) to 6.07 (KR), respectively. The genetic variation of KR and KY were the highest and the lowest within Korean native strains, respectively. The genetic distance results showed that Korean native chicken strains were separated with the three introduced chicken breeds clustered into another group. The lowest distance (0.149) was observed between the KR and KL breeds and the highest distance (0.855) between the KR and LK breeds. The microsatellite polymorphism data were shown to be useful for assessing the genetic relationship between Korean native strains and other foreign breeds.

• 한국미생물·생명공학회지
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• 제26권5호
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• pp.450-455
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• 1998

Alkaline protease를 대량생산하는 Aspergillus oryzae를 만들기 위하여 A. oryzae의 alkaline pretense 유전자 alpA를 고발현시키는 plasmid pTAalp를 제조하고 이 plasmid로 A. oryzae M-2-3 균주를 형질전환시켰다. 16개의 형질전환체를 얻어 이들의 protease생산성을 skim milk 분해에 의한 halo 형성능에 의하여 확인하였다. 또한 protease 생산성이 증가한 형질전환체는 pTAalp가 multi-copy로 염색체 안에 integration되어 있음을 Southern blot에 의하여 확인하였고, 이들의 배양액을 polyacrylamide gel전기영동에 의하여 분석한 결과, 형질전환체 No. 14에서는 전체 분비단백질의 80-90%가 alkaline protease 임을 알 수 있었다. 간장원료 분해실험의 결과 No. 14에 의한 원료분해액은 간장 양조용 대조균에 의한 분해액보다 TN이 증가하였으며 원료분해율도 1.4-1.5배로 증가되었다.

• KSBB Journal
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• 제27권3호
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• pp.151-156
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• 2012

Lactic acid is an important product arising from the anaerobic fermentation by lactic acid bacteria (LAB). It is used in the pharmaceutical, cosmetic, chemical, and food industries as well as for biodegradable polymer and green solvent production. The poly lactic acid (PLA) is an important material for bio-plastic manufacturing process. For PLA production by new LAB, we screened LAB isolates from shellfish. A total of 28 LAB were isolated from various shellfishes. They were all Gram positive, oxidase and catalase negative. Based on API 50CHL kit, 7 strains among the 28 isolates were identified as Lactobacillus plantarum, 6 strains as Lactobacillus delbrueckii, 5 strains as Leuconostoc mesenteroides, 3 strains as Lactobacillus brevis, 2 strains as Lactococcus lactis, 1 strain as Lactobacillus salivarius, 1 strain as Lactobacillus paracasei, 1 strain as Lactobacillus pentosus, 1 strain as Lactobacillus fermentum and 1 strain as Pediococcus pentosaceu. Also, we examined the amount of total lactic acid produced by these new strains by HPLC analysis with Chiralpak MA column. One strain E-3 from Mytilus edulis was indentified as Lactobacillus plantarum and found to produce 20.0 g/L of D-form lactic acid from 20 g/L of dextrose. Further studies are underway to increase the D-lactic acid production by E-3.

• Journal of Microbiology and Biotechnology
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• 제27권7호
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• pp.1266-1271
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• 2017

Lactobacillus fermentum strain JDFM216, isolated from a Korean infant feces sample, possesses the ability to enhance the longevity and immune response of a Caenorhabditis elegans host. To explore the characteristics of strain JDFM216 at the genetic level, we performed whole-genome sequencing using the PacBio system. The circular draft genome has a total length of 2,076,427 bp and a total of 2,682 encoding sequences were identified. Five phylogenetically featured genes possibly related to the longevity and immune response of the host were identified in L. fermentum strain JDFM216. These genes encode UDP-N-acetylglucosamine 1-carboxyvinyltransferase (E.C. 2.5.1.7), ErfK/YbiS/YcfS/YnhG family protein, site-specific recombinase XerD, homocysteine S-methyltransferase (E.C. 2.1.1.10), and aspartate-ammonia ligase (E.C. 6.3.1.1), which are involved in peptidoglycan synthesis and amino acid metabolism in the gut environment. Our findings on the genetic background of L. fermentum strain JDFM216 and its potential candidate genes for host longevity and immune response provide new insight for the application of this strain in the food industry as newly isolated functional probiotic.

• 마이크로전자및패키징학회지
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• 제17권1호
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• pp.9-17
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• 2010

본 논문은 $20^{\circ}$ 각도의 굽힘과 50 ${\mu}m$ 인장조건에서 PGA (Pin Grid Array) 패키지의 lead pin에 발생하는 von Mises 응력과 전 변형률 에너지 밀도를 lead pin 소재의 열처리 온도 조건에 따라 유한요소법을 이용하여 해석하였다. 해석결과에 따르면 lead pin의 코너부와 리드핀의 헤드부와 솔더의 경계면이 국부적으로 응력이 집중되는 가장 취약한 위치이며 lead pin의 열처리 온도가 높을수록 발생하는 최대 응력과 변형률 에너지 밀도가 낮아져서 신뢰성이 우수한 것으로 판단된다. 또한 lead pin의 코너부에 라운드가공을 하면 헤드부와 솔더의 경계면에서 발생하는 von Mises 응력과 전변형률 에너지 밀도가 감소하였다. 이와 같은 해석결과는 전 변형률 에너지 밀도가 증가할수록 솔더의 피로수명이 감소하는 연구결과에 미루어볼 때 열처리를 통해 리드핀의 기계적특성을 변경하면 PGA 패키지의 신뢰성을 향상시킬 수 있음을 의미한다. 따라서, 리드핀의 형상최적화와 열처리를 통한 최적화된 소재특성을 통해 PGA 패키지의 신뢰성 향상이 요구된다.

• 한국철도학회:학술대회논문집
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• 한국철도학회 2011년도 춘계학술대회 논문집
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• pp.277-282
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• 2011

Bogie frame is typical safety part of railway vehicle. Total serviceable life of bogie frame will be evaluated by Cumulative Damage Approach Method that is defined by dynamic strain measurement during revenue service under the actual track conditions. As a result of the standardization process developed in British Standard Institution, BS 7608 defines for fatigue design and test method of steel structure by fatigue test results over the long period. This paper evaluates the total serviceable life applying BS 7608 for the bogie frame of Electric Multiple Unit to verify structural safety of the bogie frame.

• 소성∙가공
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• 제18권6호
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• pp.500-507
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• 2009

Tensile deformation behavior with different strain rate was investigated. $Zr_{56.2}Ti_{13.8}Nb_{5.0}Cu_{6.9}Ni_{5.6}Be_{12.5}$(bulk metallic glass alloy possessed crystal phase which was called $\beta$-phase of dendrite shape, mean size of $20{\sim}30{\mu}m$ and occupied 25% of the total volume) was used in this study. Maximum tensile strength was obtained as 1.74GPa at strain rate $10^2s^{-1}$ and minimum strength was found to be 1.6GPa at $10^{-1}s^{-1}$. And then, maximum plastic deformation occurred at the strain rate of $5{\times}10^{-2}s^{-1}$ and represented 1.75%, though minimum plastic deformation showed 0%. In the specific range of strain rate, relatively higher plastic deformation and lower ultimate tensile strength were found with lots of shear bands. The fractographical observation after tensile test indicated that vein like pattern on the fracture surface was well developed especially in the above range of strain rate.

• 한국철도학회:학술대회논문집
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• 한국철도학회 2008년도 춘계학술대회 논문집
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• pp.109-117
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• 2008

In this study, a method predicting the displacement responseof structures from the measured dynamic strain signal is proposed by using a mode decomposition technique. Dynamic loadings including wind and seismic loadings could be exerted to the bridge. In order to examine the bridge stability against these dynamic loadings, the prediction of displacement response is very important to evaluate bridge stability. Because it may be not easy for the displacement response to be acquired directly on site, an indirect method to predict the displacement response is needed. Thus, as an alternative for predicting the displacement response indirectly, the conversion of the measured strain signal into the displacement response is suggested, while the measured strain signal can be obtained using fiber optic Bragg-grating (FBG) sensors. To overcome such a problem, a mode decomposition technique was used in this study. The measured strain signal is decomposed into each modal component by using the empirical mode decomposition(EMD) as one of mode decomposition techniques. Then, the decomposed strain signals on each modal component are transformed into the modal displacement components. And the corresponding mode shapes can be also estimated by using the proper orthogonal decomposition(POD) from the measured strain signal. Thus, total displacement response could be predicted from combining the modal displacement components.

• 한국소성가공학회:학술대회논문집
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• 한국소성가공학회 2009년도 춘계학술대회 논문집
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• pp.353-354
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• 2009

Tensile deformation behavior with different strain rate was investigated. $Zr_{56.2}Ti_{13.8}Nb_{5.0}Cu_{6.9}Ni_{5.6}Be_{12.5}$ (bulk metallic glass alloy possessed crystal phase which was called $\beta$-phase of dendrite shape, mean size of $20{\sim}30{\mu}m$ and occupied 25% of the total volume) was used in this study. Maximum tensile strength was obtained as 1.74Gpa at strain rate of $10^2/s$ and minimum strength was found to be 1.6GPa at $10^{-1}/s$. And then, maximum plastic deformation occurred at the strain rate of $5{\times}10^{-2}/s$ and represented 1.75%, though minimum plastic deformation showed 0%. In the specific range of strain rate, relatively higher plastic deformation and lower ultimate tensile strength were found with lots of shear bands. The fractographical observation after tensile test indicated that vein like pattern on the fracture surface was well developed especially in the above range of strain rate.

• 대한기계학회논문집
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• 제18권5호
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• pp.1192-1202
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• 1994

Fatigue behavior and life prediction were presented for thermal-mechanical and isothermal low cycle fatigue of 12Cr forged steel used for high temperature applications. In-phase and out-of-phase thermal-mechanical fatigue test at 350 to 600.deg. C and isothermal low cycle fatigue test at 600.deg. C were conducted using smooth cylindrical hollow specimen under strain-control with total strain ranges from 0.006 to 0.015. Cyclic softening behavior was observed regardless of thermal-mechanical and isothermal fatigue tests. The phase difference between temperature and strain in thermal-mechanical fatigue resulted in significantly shorter fatigue life for out-of-phase than for in-phase. The difference in fatigue lives was dependent upon the magnitudes of inelastic strain ranges and mean stresses. Increase in inelastic strain range showed a tendency of intergranular cracking and decrease in fatigue life, especially for out-of-phase thermal-mechanical fatigue. Thermal-mechanical fatigue life prediction was made by partitioning the strain ranges of the hysteresis loops and the results of isothermal low cycle fatigue tests which were performed under the combination of slow and fast strain rates. Predicted fatigue lives for out-of-phase using the strain range partitioning method showed an excellent agreement with the actual out-of-phase thermal-mechanical fatigue lives within a factor of 1.5. Conventional strain range partitioning method exhibited a poor accuracy in the prediction of in-phase thermal-mechanical fatigue lives, which was quite improved conservatively by a proposed strain range partitioning method.