• Asian-Australasian Journal of Animal Sciences
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• v.30 no.8
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• pp.1117-1123
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• 2017

Objective: This study was conducted to compare growth performance, blood components and carcass traits by two feeding systems (concentrate with roughage separately [CON] vs total mixed ration [TMR]) in Hanwoo steers, and to learn the relationship between blood components during fattening or finishing phases and carcass traits in Hanwoo steers. Methods: Sixty steers aged 8 months were allotted to two feeding systems and fed similar amounts of average dry matter and total digestible nutrient throughout whole experimental period according to each feeding program. Steers were weighed monthly, taken blood at the end of growing, fattening and finishing periods, and slaughtered at 30 month of age. Results: Growing performance was higher (p<0.05) in the CON group compared to the TMR group during fattening and finishing periods. The CON group was lower (p<0.05) in blood aspartic acid transaminase, blood urea nitrogen and retinol levels during growing period, but higher in triglyceride and cholesterol levels during fattening and finishing periods compared to the TMR group. The CON group was greater (p<0.05) in rib-eye area, and lighter (p<0.05) red in meat color compared to the TMR group. In the correlation coefficients between blood components of steers and carcass traits, retinol had a negative (p<0.05) correlation with marbling score and rib-eye area. Leptin had a positive (p<0.05) correlation with back fat thickness. Blood cholesterol and triglyceride were positively (p<0.05) correlated with carcass weight and rib-eye area. Conclusion: Growth performance, carcass ribeye area and meat color showed a more desirable result in the CON compared to the TMR in Hanwoo steers. Assessing the accumulated data of carcass traits with blood components including hormones-particularly retinol, cholesterol, triglyceride, and leptin-during the fattening or finishing phases, it may be possible to find a biomarker for determining beef quality in living animals.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.3
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• pp.382-389
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• 2002

The aims of this study is to evaluate the effect of Aspergillus oryzae Fermentation Extract (AFE) on the performance of lactating cows in summer (May to July) and winter (December to February). The experiment was a completely randomized design (CRD) and dietary treatments were 1) basal diet without AFE, 2) basal plus 3 g/d AFE into the basal total mixed ration (TMR), 3) basal plus 45.4 mg AFE/kg the ensiling corn silage and 4) AFE inclusion in silage and TMR. Twenty-eight cows from each trial were selected and randomly allocated into the four treatment groups, confined in individual pens, and fed ad libitum for 8 weeks in both seasons of feeding trials. Results showed that AFE inclusion in corn silage significantly improved DM intake by 4.4% and milk yield by 3.1% (p<0.05) during summer. In the winter season, AFE inclusion in the diet significantly improved milk yield by 10%. Direct addition of AFE to the TMR even further significantly improved milk yield over the addition through corn silage by 7.4% in winter (p<0.05). An additive effect of AFE inclusion into TMR and through corn silage was also demonstrated in the winter-feeding. AFE inclusion however, did not improve DM intake during the winter trial. In the summer trial, inclusion of AFE showed an adverse effect on the percentage of milk fat, but did not impact on the milk fat yield. Adding AFE through corn silage showed a trend towards alleviating the negative effects of milk fat from direct AFE inclusion in TMR. The similar trend occurred in the winter trial. The inclusion of AFE through corn silage significantly lowered the milk protein content over direct AFE addition, but did not significantly impacted the milk protein yield in summer. AFE supplementation during the winter season significantly increased milk protein content. Adding AFE to the corn silage significantly increased milk protein content over direct AFE addition in winter although inclusion of AFE significantly decreased total milk solid content in the summer (p<0.005). During the winter season, inclusion of AFE required less DM to produce a unit of milk. Inclusion of AFE into corn silage required less DM, energy and protein to produce a unit of milk. But inclusion of AFE did not alleviate heat stress on the lactating cows.

• Journal of The Korean Society of Grassland and Forage Science
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• v.27 no.3
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• pp.197-208
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• 2007

This study was carried out to investigate effects of increasing moisture content with or without supplementing inoculant (Lactobacillus plantarum) in TMR (total mixed ration) on its feed value. In case of exposing TMR to air, the lower the moisture level of TMR was, the less its apparent condition was changed. The time of spreading of molds tended to be faster in TMR with the higher moisture level. And also the odor was influenced by moisture content and inoculant supplement that is, sour odor was smelled from 24 hour after exposing TMR containing 35% and 50% moisture to air, but TMR supplemented with inoculant had sweet odor. The inner temperature of TMR containing 35% and 50% moisture without inoculant tended to increase continually after the lapse of 6 hours when the TMR was exposed to air. The inner temperature of TMR containing 35% moisture with inoculant tended to increase dramatically after the lapse of 48 hours when exposed to air, but that of TMR containing 50% moisture with inoculant tended to increase after 6 hours. The pH of TMR containing 15% was consistent regardless of exposing time to air, but that of TMR containing 35% and 50% moisture considerably increased after 12 and 24 hours, respectively. The concentration of $NH_3-N$ of TMR supplemented with inoculant was increased from 6 hours after exposure to air, while that or TMR without inoculant increased from 12 hours. Nutrient content or TMR tended to be increased with the increase of exposing time to air and storage time under sealed condition.

• Journal of the Korean Society of Food Science and Nutrition
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• v.40 no.5
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• pp.731-737
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• 2011

TMR (total mixed ration) feed was developed by adding mugwort (Artemisia capillaris Thunb.) and was fed to Hanwoo cattle. We investigated the effects of feeding mugwort on the physicochemical properties and sensory scores of the Hanwoo beef, as well as the feasibility of producing beef with high quality and function. Samples included Hanwoo loin fed with fattening cattle TMR feed with (treatment) or without mugwort (control). The content of total catechin in Hanwoo loin fed with the treatment and control was 0.516 and 0.307 mg/kg, respectively, while the content of epicatechin was 0.116 and 0.087 mg/kg, respectively, both of which were significant increase from feeding TMR with mugwort (p<0.05). There was no significant difference between the control and treatment in terms of $b^*$ value, VBN content, EDA, total bacterial numbers, freezing loss, thawing loss, cooking loss, hardness, cohesiveness, gumminess, chewiness, shear force, or sensory score of boiled meat. $L^*$, $a^*$ and pH value for the treatment were significantly higher than the control (p<0.05). TBARS and springiness for the control were significantly higher than the treatment (p<0.05). The aroma of the raw meat and the taste, tenderness, juiciness, and palatability of the roasted meat for the treatment were significantly superior to the control (p<0.05). These results suggest that giving feed containing mugwort inhibits lipid oxidation, increases water holding capacity, and improve sensory scores.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.9
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• pp.1374-1388
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• 2007

Three experiments were conducted to investigate the hypothesis that cows grazing on pasture produce the highest proportion of c-9 t-11 CLA in milk fat and no further increase can be achieved through supplementation of diets rich in linoleic acid, such as full-fat extruded soybeans or soybean oil. In experiment 1, 18 lactating Holstein cows were used in a randomized complete block design with measurements made from wk 4 to 6 of the experiment. In experiment 2, three cannulated lactating Holstein cows were used in a $3{\times}3$ Latin square design. Each period was 4 wk with measurements made in the final wk of each period. Cows in both experiments were assigned at random to treatments: a, conventional total mixed ration (TMR); b, pasture (PS); or c, PS supplemented with 2.5 kg/cow per day of full-fat extruded soybeans (PES). In both experiments, feed intake, milk yield, milk composition, and fatty acid profile of milk and blood serum were measured, along with fatty acid composition of bacteria harvested from rumen digesta in experiment 2. In experiment 3, 10 cows which had continuously grazed a pasture for six weeks were assigned to two groups, with one group (n = 5) on pasture diet alone (PS) and the other group (n = 5) supplemented with 452 g of soy oil/cow per day for 7 d (OIL). In experiment 1, cows in PS treatment produced 350% more c-9, t-11 CLA compared with cows in TMR treatment (1.70 vs. 0.5% of fat), with no further increase for cows in PES treatment (1.50% of fat). Serum c-9, t-11 CLA increased by 233% in PS treatment compared with TMR treatment (0.21 vs. 0.09% of fat) with no further increase for cows in PES treatment (0.18% of fat). In experiment 2, cows in PS treatment produced 300% more c-9 t-11 CLA in their milk fat compared with cows in TMR treatment (1.77 vs. 0.59% of fat), but no further increase for cows in PES treatment (1.84% of fat) was observed. Serum c-9, t-11 CLA increased by 250% for cows in PS treatment compared with cows in TMR treatment (0.27 vs. 0.11% of fat), with no further increase for cows in PES treatment (0.31% of fat). The c-9, t-11 CLA content of ruminal bacteria for cows in PS treatment was 200% or more of TMR treatment, but no further increase in bacterial c-9, t-11 CLA for cows in PES treatment was observed. Supplementation of soy oil in experiment 3 also did not increase the c-9 t-11 CLA content of milk fat compared with cows fed a full pasture diet (1.60 vs. 1.54% of fat). Based on these findings, it was concluded that supplementing with feeds rich in linoleic acid, such as full-fat extruded soybeans or an equivalent amount of soy oil, to cows grazing perennial ryegrass pasture may not increase milk fat c-9 t-11 CLA contents.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.4
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• pp.495-504
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• 2017

Objective: Manipulating the fermentation to improve the performance of the ruminant has attracted the attention of both farmers and animal scientists. Propionate salt supplementation in the diet could disturb the concentration of propionate and total volatile fatty acids in the rumen. This study was conducted to evaluate the effect of calcium propionate supplementation on the ruminal bacterial community composition in finishing bulls. Methods: Eight finishing bulls were randomly assigned to control group (CONT) and calcium propionate supplementation (PROP) feeding group, with four head per group. The control group was fed normal the total mixed ration (TMR) finishing diet, and PROP group was fed TMR supplemented with 200 g/d calcium propionate. At the end of the 51-day feeding trial, all bulls were slaughtered and rumen fluid was collected from each of the animals. Results: Propionate supplementation had no influence the rumen fermentation parameters (p>0.05). Ruminal bacterial community composition was analyzed by sequencing of hypervariable V3 regions of the 16S rRNA gene. The most abundant phyla were the Firmicutes (60.68%) and Bacteroidetes (23.67%), followed by Tenericutes (4.95%) and TM7 (3.39%). The predominant genera included Succiniclasticum (9.43%), Butyrivibrio (3.74%), Ruminococcus (3.46%) and Prevotella (2.86%). Bacterial community composition in the two groups were highly similar, except the abundance of Tenericutes declined along with the calcium propionate supplementation (p = 0.0078). Conclusion: These data suggest that the ruminal bacterial community composition is nearly unchanged by propionate supplementation in finishing bulls.

• Journal of The Korean Society of Grassland and Forage Science
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• v.33 no.3
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• pp.213-218
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• 2013

This study was conducted to investigate the effects of supplementing additional sucrose, in the form of black sugar (BS), into the diet of Holstein dairy cows on dry matter intake (DMI), milk yield, and milk composition. Eight Holstein dairy cows ($741{\pm}65.8kg$ body weight) were divided into two groups, including the control and BS groups. Animals in the control group were offered a total mixed ration (TMR) ad libitum, and the BS group was offered TMR with 300 g of BS/head/d. After two weeks of adaptation period, the animal performance, including DMI, milk yield and milk composition, was measured. Cows supplemented with BS appeared to consume more feed than that by the controls (i.e., 17.08 and 18.28 kg/d for the control and BS groups, respectively). However, there were no significant differences between treatments. Milk yield or milk composition, such as milk fat, milk protein, lactose, solids-non-fat, total solids and pH, did not differ between treatments. However, there was a significant difference (p<0.05) in the concentration of milk urea nitrogen (MUN). The MUN concentration of the BS group was approximately 15% lower than that of the control group (i.e., 18.75 vs. 16.05 mg/dL for the control and BS groups, respectively), which suggests improved nitrogen metabolism in the animals. The somatic cell count was numerically lower in the cows of the BS group compared to those in the control group. However, a significant difference was not noted due to the substantial amount of variation among cows. In terms of the trace mineral composition for milk, the concentration of Cu from BS animals was higher (p<0.05) than that of the control animals. In summary, supplementing the diets of dairy cows with BS marginally affected animal performance and improved nitrogen metabolism. The level of supplementation and other factors, such as animal variation were discussed.

• Journal of The Korean Society of Grassland and Forage Science
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• v.38 no.4
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• pp.291-297
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• 2018

This study was conducted to investigate the effects of feeding system and NDF levels of TMR on blood metabolites in Holstein heifers. Fifty heifers were assigned to one of five treatments according to feed type and level of NDF: TMR (total mixed ration), CON (concentration + mixed forage), T1 (${\geq}53%$ NDF), T2 (50~53% NDF), and T3 (${\leq}50%$ NDF). Although GLU concentrate was not effected by feed type, increased significantly as the level of NDF decreased. There was no effect on concentrate of blood metabolite related with protein. As the level of NDF decreased, the blood ALB concentration was increased (p<0.05). The concentration of blood CHOL was higher in CON than those of TMR (p<0.05), and increased as the level of NDF decreased. The concentration of blood lipid was higher in CON than those of TMR and increased at lower level of NDF. The concentration of blood ALT were significantly lower in T3 than T1 and T2 (p<0.05). Thus, the results of this study suggest that the feeding system and NDF level may affect the blood metabolite concentration; however, the feed intake and other nutrient levels should also be considered.

• Korean Journal of Organic Agriculture
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• v.24 no.3
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• pp.485-495
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• 2016

The aim of the present study was to determine the effect of dietary cultured wild ginseng root (CWGR) supplementation on goat milk composition and ginsenoside profiles. Sixteen Saanen dairy goats were allocated to two balanced groups based on lactation period, body weight ($38.6{\pm}3.2kg$), and dairy milk yield ($2.85{\pm}1.2kg$), and were kept in separate pens. Goats were fed a total mixed ration (TMR) feed (2.3 kg/d, dry matter basis) and 1.5 g of CWGR powder was supplemented in the experimental diet. The total feeding period was 3 weeks, and milk and blood samples were collected on the last three days of the experimental period. There was no effect of CWGR on daily milk yield and milk composition (fat, protein, lactose, and solid-not-fat). However, the CWGR-treatment group had significantly higher plasma IgG and protein contents than the control group (P < 0.05). Significant amounts of ginsenosides were observed in the milk of the CWGR-treatment group, whereas ginsenosides were not detected in the milk of the control group. In conclusion, dietary CWGR was a useful regimen to produce functional goat milk enriched in ginsenosides.

• Journal of The Korean Society of Grassland and Forage Science
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• v.34 no.4
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• pp.296-306
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• 2014

This study was conducted to survey and analyze the quantity of various organic wastes and to vitalize the utilization of agri-food by-products as raw materials for Total mixed ration (TMR), to improve feed cost savings and the quality of animal products. On-the-spot obstacles for animal farmers, along with legal and institutional alternatives are presented. The results are as follows. First, organic wastes in Korea are managed by the Allbaro system created in the Wastes Control Act, which processes 10,488 tons of cooking oil waste, 832,493 tons of animal and plant residues, 5,740 tons of animal carcasses, 1,171,892 tons of animal residues, and 2,172,415 tons of plant residues including 12,905 tons of rice hull and bran, for a total of 4,205,931 tons. Raw materials for TMR, namely rice hulls and bran as well as plant residues, accounted for 51.7% of the total national organic waste. The top 10 municipalities process 76~100% of all organic wastes and a supply management system is needed for the waste. Second, the 10 major agri-food by-products used as raw materials for TMR are bean curd by-product, rice bran, oil-cake, brewers dried grain, Distiller's Dried Grains with Solubles (DDGS), barley bran, soy sauce by-product, citrus fruit by-product, mushroom by-product and other food by-product (bread, noodles, snacks, etc.). Third, the biggest difficulties in using agri-food by-products are legal obstacles. Because agri-food by-products are regulated as industrial wastes by the Waste Control Act, animal farmers that wish to use them have legal reporting obligations including the installation of recycling facilities. To enable the use of agri-food by-products as raw materials for TMR, waste management system improvements such as 'the end of waste status' and the establishment of more than 10 public distribution centers nationwide are deemed essential.