• The Journal of Korean Academy of Prosthodontics
• /
• v.52 no.2
• /
• pp.74-81
• /
• 2014

Purpose: While studies have examined microleakage in endodontically treated teeth restored with posts, microleakage among post and adhesive systems remains a concern. This study compared the sealing properties of 3 adhesively luted post systems. Materials and methods: Thirty-six endodontically treated permanent maxillary central incisors were divided into 3 groups: Zirconia-glass fiber, Quartz-glass fiber, Polyethylene fiber posts. Post space was prepared and each post was adhesively luted with 3 systems. The specimens were separately immersed in freshly prepared 2% methylene blue solution for 1 week. The cleaned specimens were then embedded in autopolymerizing acrylic resin. The root portion of tooth were horizontally sectioned into three pieces (apical, middle, and coronal portions). An occlusal view of each section was digitally photographed with a stereomicroscope. The methylene blue-infiltrated surface for each specimen was measured. Dye penetration was estimated as the ratio of the methylene blue-infiltrated surface to the total dentin surface. Results: No significant differences were found among post types. The variables of middle section and 3-stage adhesive produced significant differences in microleakage between the following post pairs: zirconia-glass fiber versus quartz-glass fiber, zirconia-glass fiber versus polyethylene fiber, and quartz-glass fiber versus polyethylene fiber (P<.05). There were significant differences between the apical and coronal sections of each post type, and between apical versus middle sections of quarze-glass fiber and polyethylene fiber posts (P<.05). Conclusion: No significant differences were found among post types. The 3-stage adhesive produced significant differences in microleakage between the following post pairs.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.34 no.2
• /
• pp.204-214
• /
• 2007

The aim of this study was to investigate whether differences in surface treatment prior to repair influenced the seal of a resin fissure sealant placed on the occlusal surfaces of permanent molar teeth. One hundred and twenty extracted human molars were selected and a light curing sealant was placed on their occlusal surface following cleaning by prophylaxis and acid etching. The teeth were deliberately damaged, and then stored in artificial saliva for four weeks. Sixty teeth with the desired morphology of sealant failure were randomly divided into four groups where one group was treated with only etching agent, another by only air-abrasion, another by applying an etching agent followed by a bonding agent, and the last by air-abrasion followed by a bonding agent each. After sealant application, the samples were thermocycled and the degree of microleakage was determined. The results were as follows : 1. In the sealant/sealant interface group 2(0.22), 4(0.23) using air-abrasion showed a significantly lower microleakage score than group 1(0.38), 3(0.35) using an etching agent(p<0.05). There were no statistically significant differences(p>0.05) between groups 1, 2 and groups 3, 4. 2. In the sealant/tooth interface, group 3(0.20), 4(0.20) which used a bonding agent showed a significantly lower microleakage score than group 1(0.35), 2(0.40) (p<0.05). There were no statistically significant differences(p>0.05) between groups 1, 3 and groups 2, 4. 3. In SEM examination, while sealant surfaces treated with etching did not result in highly rough surfaces, those treated with air-abrasion did show rough surfaces.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.29 no.4
• /
• pp.632-640
• /
• 2002

The objective of the study was to apply the vibration technique to reduce the viscosity of bonding adhesives and thereby compare the bond strength and resin penetration into dentinal tubules achieved with those gained using the conventional technique. Eighty-eight noncarious extracted human permanent molar teeth were sectioned to remove the coronal enamel and were embedded in 1-inch PVC pipe with acrylic resin. The occlusal surfaces were placed so that the tooth and the embedding medium were at the same level to form one flat surface, and the samples were subsequently polished with silicon carbide abrasive papers. The samples were randomly assigned to 4 groups(n=22). On Group 1 and 2, Single Bond(3M-ESPE, St. Paul, USA) was used, and on Group 3 and 4, One-Step(Bisco Inc., Schaumburg, USA) was used, and each was applied according to its manufacturer's instructions. For Group 2 and Group 4, vibration was applied with ultrasonic scaler for 10 seconds, and the adhesive was light-cured for 10 seconds. Resin composite was condensed on to the prepared surface in two increments using a mold kit(Ultradent Products Inc., USA) and each was light-cured for 40 seconds. After 24 hours in tap water at room temperature the specimens were thermocycled, and shear bond strengths were measured with a universal testing machine(Instron 4465, Canton, USA). To investigate infiltration patterns of the adhesive materials, the surface of specimen was examined with scanning electron microscope. The results were as follows. 1. The shear bond strengths of vibration groups(Group 2, Group 4) were significantly greater than those of the non-vibration groups(Group 1, Group 3)(p<0.05). 2. The shear bond strengths of Single Bond and One-Step were not significantly different (p>0.05). 3. The vibration groups showed greater number of resin tags in tubules and lateral branches under SEM.

• The korean journal of orthodontics
• /
• v.27 no.1
• /
• pp.157-171
• /
• 1997

This study was conducted to see the effect of TiN ion-plating on the bond strength of orthodontic bracket. Three stainless-steel brackets with different base types were chosen; when TiN ion-plated brackets and non iorrplated brackets were bonded to the teeth, initial and long-term bond strength were measured, The observations oi bonding surface and failure sites through the scanning electron microscope were analysed and compared. The summary of this study was as follows; ${\cdot}$ When TiN ion-plating was not applied, the Micro-Loc type was the highest in bond strength atter 24 hours as $5.89{\pm}1.77$ MPa, followed by $4,27{\pm}1.12MPa$ for Foil Mesh type and $2.64{\pm}0.58MPa$ for Undercut type(P<0.05). ${\cdot}$ Under TiN ion-plating, the bond strength after 24 hours showed: Micro-Loc type $-6.26{\pm}1.51MPa$, Foil Mesh type $-7.45{\pm}2.01MPa$, Undercut type $-2.93{\pm}0.84MPa$. Unlike in the case of non ion-plating, Foil Mesh type showed a higher strength than Micro-Loc type, with Undercut type still showing the lowest bond strength(P<0.05). The bond strength, after 24 hours, increased in case of ion-plated in all 3 types, but a significant increase was shown only in Foil Mesh type(P<0.001). ${\cdot}$ Under a long-term immersion, regardless of ion or non ion-plating, bond strength in general increased over the initial bond strength(one day), with more stability. ${\cdot}$ Through scanning electron microscopic observation of bonding surface, it was found that, regardless of the bracket base type or the application of ion-plating, the resin was thoroughly spreaded into bracket base to form a solid bonding surface between the bracket and the tooth. This was also true in case of a long-term immersion. ${\cdot}$ The scanning electron microscopic observation of failure sites revealed diverse failure patterns.

• The korean journal of orthodontics
• /
• v.30 no.5 s.82
• /
• pp.599-611
• /
• 2000

Alveolar crest is the section of interproximal alveolar bone which includes the free edge of the alveolar process. An increase of the normal forces within limits of tolerance leads to deposition of new bone. If forces are beyond the limits of tolerance, resorption of bone will result whether the force produces pressure or tension. This study was designed to evaluate changes of alveolar bone levels in mesial and distal surface of the left, right first molar, by using pre-treatment, post-treatment panorama films. Two hundreds sixteen subjects were divided into adolescent group of 104 subjects and adult group of 112 subjects, to which orthodontic treatment with a bicuspid extraction (adolescent group-50 subjects, adult group-50 subjects) or without a nonextraction (adolescent group-54 !subjects, adult group-62 subjects) was applied by fixed appliances. Pre- and post-treatment Panorama films were traced, and alveolar crest height was measured. Amounts of changes in alveolar crest height by treatment were calculated md compared in terms of side of tooth, extraction, age. The results were as follows ; 1. When pre-treatment alveolar crest bone levels were compared, levels of adult group were significantly lower than those of adolescent group. 2. Post-treatment alveolar crest bone levels were significantly lower than pre-treatment levels. 3. When changes of alveolar crest height were compared, between adolescent and adult group were not significantly. 4. When changes of alveolar crest height were compared, significantly larger changes were noticed in ex윤action than nonextraction cases. 5. When changes of alveolar crest height were compared, significantly larger changes were noticed in maxilla than mandible. 6. When mesio-distally compared, significantly larger changes were observed in the distal than mesial sides of adult group.

• The korean journal of orthodontics
• /
• v.36 no.4
• /
• pp.308-320
• /
• 2006

Rapid canine retraction, first introduced by Liou, is a distraction osteogenesis applied to the periodontal ligament tissue. Rapid tooth movement was facilitated by establishing minimal bony resistance on the distal surface of the canine by socket preparation and by osteogenesis on the mesial side in response to the periodontal distraction. Since undesired buccal tipping or extrusion of the canine during retraction tends to occur, it is crucial to maintain the firm path of movement and the axis of the canine during retraction. In order to improve the predictability of the canine movement, lingually extended distraction screws with heavy labial guiding wires were designed. Prefabricated plastic canine models for the estimation of socket depth and miniscrew implants for anchorage reinforcement were also devised. Applying these devices to a female patient with Class II anterior protrusion, the whole treatment was effectively finished in 13 months. Loss of vitality or periodontal problems did not occur throughout treatment, and stable occlusion was maintained during 10 months of retention. This case report demonstrates that a predictable rapid canine retraction can be achieved through the use of this modified technique.

• The korean journal of orthodontics
• /
• v.24 no.3 s.46
• /
• pp.659-672
• /
• 1994

Dental caries is one of the most prevalent dental diseases in Korea and its prevention is very important in orthodontic therapy. For the cleansing action of saliva itself and/or tooth-brushing is lowered in patient with fixed orthodontic appliance, oral hygiene of the patient becomes worse, which provides more favorable environment for micro-organisms. Chlorhexidine, one of the series of bisguanide, has been reported to be strong antimicrobial agent and very effective on Streptococcus mutans. The purpose of this study is to evaluate the possibility of chlorhexidine as a anticariogenic agent in fixed orthodontic therapy. We used the varnish containing chlorhexidine as a main ingredient for the chemical control of salivary S.mutans in patients with fixed appliance therapy We applied the varnish containing chlorhexidine on the labial and interproximal surface of the teeth before bonding and banding teeth of our patients(N=20) and compared to control group patients(N=20). Before the application of chlorhexidine varnish and four times periodically after the completion of fixed appliance set-up, we sampled saliva of both group patients and incuvate S.mutans for 24 hours. In the culture study of sampled saliva, counting the number of S.mutans colonies, we founded as follows : 1. In patients with fixed appliance therapy, the risk of dental caries increase when it compared to that of preorthodontic treatment ; The number of salivary S.mutans increase in Patient's oral cavity. 2. The experimental agent that contain chlorhexidine is effective to reduce the number of salivary S.mutans. 3. For the effect of this agent is not ever-lasting, periodical application is needed, and additional study for economical interval and number of application is needed.

• Journal of dental hygiene science
• /
• v.12 no.2
• /
• pp.155-162
• /
• 2012

Somok, the heart wood of Caesalpinia sappan is used in traditional Chinese medicine. Adherence of S. mutans to the tooth surface can result in the formation of a dental plaque. This study was performed to investigate the antibacterial activity and bacterial adhesion of ethyl acetate extract from C. sappan against S. mutans ATCC 25175. The bacteria were cultured in brain heart infusion(BHI) broth, and then incubated under 5% $CO_2$ at $37^{\circ}C$ for 18~24 hours. The antimicrobial activity of the ethyl acetate extract of C. sappan was then examined using the paper disc methods and MIC. In addition, bacterial adherence to hydroxyapatite was also examined. The ethyl acetate extract was shown to produce inhibitory effects and had MIC values of 125 mg/ml against S. mutans ATCC 25175. The ethyl acetate extract inhibited adhesion of S. mutans to saliva coated-hydroxyapatite beads(S-HA). At 24 hr, the ethyl acetate extract significantly reduced the adherence of S. mutans to S-HA beads relative to the control. The isolated active substance was identified as brazilin($C_{16}H_{14}O_5$) by $^1H-NMR$ and $^{13}C-NMR$. Thus, the application of C. sappan can be considered a useful and practical method for the prevention of dental caries.

• Molecules and Cells
• /
• v.39 no.11
• /
• pp.790-796
• /
• 2016

Dental pulp is a highly vascularized tissue requiring adequate blood supply for successful regeneration. In this study, we investigated the functional role of stem cells from human exfoliated deciduous teeth (SHEDs) as a perivascular source for in vivo formation of vessel-like structures. Primarily isolated SHEDs showed mesenchymal stem cell (MSC)-like characteristics including the expression of surface antigens and in vitro osteogenic and adipogenic differentiation potentials. Moreover, SHEDs were positive for NG2, ${\alpha}$-smooth muscle actin (SMA), platelet-derived growth factor receptor beta ($PDGFR{\beta}$), and CD146 as pericyte markers. To prove feasibility of SHEDs as perivascular source, SHEDs were transplanted into immunodeficient mouse using Matrigel with or without human umbilical vein endothelial cells (HUVECs). Transplantation of SHEDs alone or HUVECs alone resulted in no formation of vessel-like structures with enough red blood cells. However, when SHEDs and HUVECs were transplanted together, extensive vessel-like structures were formed. The presence of murine erythrocytes within lumens suggested the formation of anastomoses between newly formed vessel-like structures in Matrigel plug and the host circulatory system. To understand underlying mechanisms of in vivo angiogenesis, the expression of angiogenic cytokine and chemokine, their receptors, and MMPs was compared between SHEDs and HUVECs. SHEDs showed higher expression of1VEGF, SDF-$1{\alpha}$, and $PDGFR{\beta}$ than HUVECs. On the contrary, HUVECs showed higher expression of VEGF receptors, CXCR4, and PDGF-BB than SHEDs. This differential expression pattern suggested reciprocal interactions between SHEDs and HUVECs and their involvement during in vivo angiogenesis. In conclusion, SHEDs could be a feasible source of perivascular cells for in vivo angiogenesis.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.43 no.4
• /
• pp.347-353
• /
• 2016

The purpose of this study was to evaluate shear bond strength and microleakage of a new self-etch fissure sealant containing S-PRG filler (BeautiSealant). Fourty-five premolars were randomly divided into three groups: SEPBS group (Self-etching primer + BeautiSealant), AEBS group (Phosphoric acid etching + BeautiSealant), AECP group (Phosphoric acid etching + $Clinpro^{TM}$ sealant). For the shear bond strength test, microcylinders of fissure sealant were bonded to prepared buccal surfaces of teeth and shear bond strengths were measured by a universal testing machine. For the microleakage test, sealants were applicated on each occlusal fissure of teeth. All teeth surface were coated with finger nail varnish, with the exception of a 1.0 mm window around the sealant margins. The teeth were immersed in 2% methylene blue solution for 24 hours and then rinsed in water. The teeth were then embedded in resin and cut buccolingually along the tooth axis and observed with a stereomicroscope to determine the degree of microleakage. SEPBS group demonstrated lower bond strength in comparison with that of AEBS and AECP groups (p < 0.05). However, no significant differences were observed between AEBS and AECP groups. There was no statistically significant difference in degree of microleakage among three groups.