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Antimicrobial Activities and Adherence Inhibition on Streptococcus mutans by Ethyl Acetate Extract from Caesalpinia sappan L.  

Kwon, Hyun-Jung (Dept. of Biology, College of Natural Science, Soonchunhyang University)
Kim, Yong-Hyun (Dept. of Biology, College of Natural Science, Soonchunhyang University)
Han, Kook-Il (Dept. of Biology, College of Natural Science, Soonchunhyang University)
Jeon, Mi-Ae (Dept. of Biology, College of Natural Science, Soonchunhyang University)
Han, Man-Deuk (Dept. of Biology, College of Natural Science, Soonchunhyang University)
Publication Information
Journal of dental hygiene science / v.12, no.2, 2012 , pp. 155-162 More about this Journal
Abstract
Somok, the heart wood of Caesalpinia sappan is used in traditional Chinese medicine. Adherence of S. mutans to the tooth surface can result in the formation of a dental plaque. This study was performed to investigate the antibacterial activity and bacterial adhesion of ethyl acetate extract from C. sappan against S. mutans ATCC 25175. The bacteria were cultured in brain heart infusion(BHI) broth, and then incubated under 5% $CO_2$ at $37^{\circ}C$ for 18~24 hours. The antimicrobial activity of the ethyl acetate extract of C. sappan was then examined using the paper disc methods and MIC. In addition, bacterial adherence to hydroxyapatite was also examined. The ethyl acetate extract was shown to produce inhibitory effects and had MIC values of 125 mg/ml against S. mutans ATCC 25175. The ethyl acetate extract inhibited adhesion of S. mutans to saliva coated-hydroxyapatite beads(S-HA). At 24 hr, the ethyl acetate extract significantly reduced the adherence of S. mutans to S-HA beads relative to the control. The isolated active substance was identified as brazilin($C_{16}H_{14}O_5$) by $^1H-NMR$ and $^{13}C-NMR$. Thus, the application of C. sappan can be considered a useful and practical method for the prevention of dental caries.
Keywords
Antimicrobial activity; Bacterial adherence; Caesalpinia sappan L.; Streptococcus mutans;
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