• Molecules and Cells
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• v.27 no.2
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• pp.225-235
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• 2009

Antibody phage display provides a powerful and efficient tool for the discovery and development of monoclonal antibodies for therapeutic and other applications. Antibody clones from synthetic libraries with optimized design features have several distinct advantages that include high stability, high levels of expression, and ease of downstream optimization and engineering. In this study, a fully synthetic human scFv library with six diversified CDRs was constructed by polymerase chain reaction assembly of overlapping oligonucleotides. In order to maximize the functional diversity of the library, a ${\beta}$-lactamase selection strategy was employed in which the assembled scFv gene repertoire was fused to the 5'-end of the ${\beta}$-lactamase gene, and in-frame scFv clones were enriched by carbenicillin selection. A final library with an estimated total diversity of $7.6{\times}10^9$, greater than 70% functional diversity, and diversification of all six CDRs was obtained after insertion of fully randomized CDR-H3 sequences into this proofread repertoire. The performance of the library was validated using a number of target antigens, against which multiple unique scFv sequences with dissociation constants in the nanomolar range were isolated.

• Korean Journal of Construction Engineering and Management
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• v.7 no.4 s.32
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• pp.177-183
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• 2006

Risk-based estimation has been successfully introduced into the construction industry. By incorporating historical data associated with probability analysis, risk-based estimate is an effective decision support aid in considering whether to launch a particular project. The industry challenges, however, especially related with management issues, such as labor shortage, wage growth, and supply chain complexity, have often resulted in poor cost performance. The insufficient assessing the project characteristics (i.e., resource availability, project complexity, and project delivery method) can be the main reasons in the poor cost performance. Because the accuracy level of cost performance prediction can be enhanced by extensive evaluation of the subject project characteristics, a new approach for predicting cost performance in an earlier stage of a project can improve the Industry substantiality, in other words, value maximization. The purpose of this paper is to develop a new methodology in developing a risk-based estimate tool by incorporating extensive project characteristics. To do this, an extensive industry survey was conducted from both private and public sectors in building industry in Korea. In addition, significant project characteristics were identified in terms of cost performance indicator. Although the data collection is limited to Korean industry the suggested approach provides the industry with a straightforward methodology in risk management. As many researchers maintained that front-end planning efforts are crucial in achieving the successful outcome in building projects, the new method for risk-based estimation can Improve the cost performance as well as enhance the fulfillment in terms of business sustainability.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.10 no.2
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• pp.338-343
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• 2006

This paper describes about the design concept and the architecture of an economic VPN system which can perform fast crypto operations with cheap cost. The essence of the proposed system architecture is consisting of the system with two companion chips dedicated to VPN: one chip is a multi-purpose network processor for security machine and the other is a crypto acceleration chip which encrypt and decrypt network packets in a high speed. This study also addresses about some realizations that is required for fast prototyping such as the porting of an operating system, the establishment of compiler tool chain, the implementation of device drivers and the design of IPSec security engine. Especially, the second chip supports the most time consuming block cipher algorithms including 3DES, AES, and SEED and its performance was evaluated.

• Annual Conference of KIPS
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• 2014.11a
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• pp.646-649
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• 2014

최근 레가시 소프트웨어 기능의 증가와 범위가 넓어져, 결함으로 발생하는 사고의 피해 규모가 증가하고 있다. 그로 인해 소프트웨어의 고품질화가 절대적으로 필요하다. 기존 NIPA의 소프트웨어 가시화는 코드로부터 아키텍처 추출을 중점을 고려한다. 이로부터 역공학 기법을 통한 객체지향 코드의 정적 분석과 가시화로 모델(클래스 모델, 순차적 모델, 패키지 모델, 그리고 유스 케이스 모델)과 요구사항을 추출하고자 한다. 이에 앞서 기존에 구축한 Tool-chain에서 가시화를 통한 클래스 모델 추출을 먼저 시도한다. 본 논문에서는 객체지향 패러다임에 맞게 수정된 결합도 측정 방법을 제안하고 추출된 UML 클래스 다이어그램에 적용한다. 그 방법은 측정된 결합도를 클래스 간 의존 관계와 비교하고 UML 클래스 다이어그램에 표현하는 것이다. 이를 통해 기존 레가시 소프트웨어의 재개발 과정에서 설계 문서의 추출과 고품질화가 가능하다.

• Food Science of Animal Resources
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• v.39 no.1
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• pp.13-22
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• 2019

This study aimed to develop a bile-responsive expression system for lactobacilli. The promoters of four genes, encoding phosphoenolpyruvate-dependent sugar phosphotransferase (mannose-specific), L-lactate dehydrogenase (LDH), HPr kinase, and D-alanine-D-alanine ligase, respectively, which were highly expressed by bile addition in Lactobacillus johnsonii PF01, were chosen. Each promoter was amplified by polymerase chain reaction and fused upstream of the ${\beta}$-glucuronidase gene as a reporter, respectively. Then, these constructs were cloned into E. coli-Lactobacillus shuttle vector pULP2, which was generated by the fusion of pUC19 with the L. plantarum plasmid pLP27. Finally, the constructed vectors were introduced into L. plantarum for a promoter activity assay. The LDH promoter showed the highest activity and its activity increased 1.8-fold by bile addition. The constructed vector maintained in L. plantarum until 80 generations without selection pressure. A bile-responsive expression vector, $pULP3-P_{LDH}$, for Lactobacillus spp. can be an effective tool for the bile-inducible expression of bioactive proteins in intestine after intake in the form of fermented dairy foods.

• Journal of the Semiconductor & Display Technology
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• v.16 no.3
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• pp.71-77
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• 2017

As the automobile industry has shifted to software, the Automotive SPICE standard has been established to ensure efficient product development process and quality. In the assessment model, the HIS Scope is the minimum standard for small and medium automotive electric companies to meet OEM requirements. However, in order to achieve the HIS Scope, the output of each process stage that meets the verification criteria of Automotive SPICE must be created. In particular, the test phase takes a lot of resources, which is a big burden for small and medium-sized companies. In this paper, we propose a methodology for creating test cases of software integration test phase based on UML sequence diagram, which is a software design phase of Automotive SPICE HIS Scope, by applying behavior model based testing method. We also propose a tool chain for automating the creation process. This will reduce the resources required to create a test case.

• Journal of Korea Planning Association
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• v.53 no.7
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• pp.23-37
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• 2018

In recent years, there has been a proliferation of commercial uses in the area where Gentrification occurs. In order to cope with such a diffusion phenomenon, it is necessary to derive the diffusion area accurately. Gentrification has a feature that occurs locally in a short time. Therefore, this study used the space-time pattern analysis to derive the diffusion area of commercial uses in Bukchon, a typical gentrified area in Seoul. Among the space-time pattern analysis, space time permutation statistics used in this study can derive the convergence phenomenon of time and space in a particular case with only the location information. The results confirmed that there has been three proliferation area in Bukchon, one started in 2007, other in 2013 and the other in 2014. In the earliest area, independent shops were the most popular, and the majority of them were restaurants. However in latter areas, there has been more chain stores and clothing stores than earlier area. The findings of this study indicate that space time pattern analysis can be used as a tool to analyze the proliferation by gentrification because it can derive accurate location and timing of the diffusion.

• Genomics & Informatics
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• v.16 no.4
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• pp.29.1-29.5
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• 2018

Hybrid capture-based targeted sequencing is being used increasingly for genomic variant profiling in tumor patients. Unique molecular index (UMI) technology has recently been developed and helps to increase the accuracy of variant calling by minimizing polymerase chain reaction biases and sequencing errors. However, UMI-adopted targeted sequencing data analysis is slightly different from the methods for other types of omics data, and its pipeline for variant calling is still being optimized in various study groups for their own purposes. Due to this provincial usage of tools, our group built an analysis pipeline for global application to many studies of targeted sequencing generated with different methods. First, we generated hybrid capture-based data using genomic DNA extracted from tumor tissues of colorectal cancer patients. Sequencing libraries were prepared and pooled together, and an 8-plexed capture library was processed to the enrichment step before 150-bp paired-end sequencing with Illumina HiSeq series. For the analysis, we evaluated several published tools. We focused mainly on the compatibility of the input and output of each tool. Finally, our laboratory built an analysis pipeline specialized for UMI-adopted data. Through this pipeline, we were able to estimate even on-target rates and filtered consensus reads for more accurate variant calling. These results suggest the potential of our analysis pipeline in the precise examination of the quality and efficiency of conducted experiments.

• Journal of the Korea Safety Management & Science
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• v.20 no.4
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• pp.1-6
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• 2018

Recently, bicycle has means of effective healthy transportation, and riding the bicycles is considered as popular recreational and sporting activities. Also, the saddle, steering system, driving device and braking device are researched briskly because of consumer's need for driving performance and comfort. Especially, the importance of a cassette responsible for transmission function by transmitting power to the drive shaft through the chain is very focused. The writer conducted structural analysis for the sprocket of each level using the ANSYS widely used for the analysis. Speed shifting performance was enhanced by minimization / simplification of shifting point through a sort of tooth profile of the cassette. By partitioning a clear value type and other shifting point, it has been modified to enable smooth speed-shifting. In addition, as titanium precision forming process, this study studied the molding technique by blanking and dies forging for mass production of the cassette. so it could be expected that the entire drive train would utilize that in the future. The stamping process capability for thin materials for the mass production of the sprockets is applicable to producing automobile parts, so lightweight component production is likely to be possible through that, for the safety of driving.

• The Journal of Internal Korean Medicine
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• v.42 no.3
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• pp.259-278
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• 2021

Objectives: This study examines the effect of Sagunja-tang on functional dyspepsia (FD) through a systematic review and meta-analysis of a randomized controlled trial (RCT). Methods: A search for RCTs that tested the effect of Sagunja-tang on functional dyspepsia was conducted in Medline, Embase, PubMed, CENTRAL, CiNii, CNKI, NDSL, RISS, OASIS, and KISS databases on November 8, 2020, with no limit on the year of publication. A meta-analysis was performed by synthesizing the findings, including total efficiency, clinical symptom score, myosin light-chain kinase (MLCK) level (pg/mL, and gastric half-emptying time (min). RevMan 5.4.1 software was used for data analysis. The quality of the literature was evaluated using Cochrane's risk of bias (RoB) tool. Results: A total of 14 RCTs met the selection criteria. As a result of the meta-analysis, the treatment group had higher total efficacy and MLCK levels (gastric antrum, jejunum) than the control group, and the clinical symptom score and gastric half-emptying time were lower. However, due to the low quality of the included RCT and the small sample size, the results may be slightly biased.