• Journal of Microbiology and Biotechnology
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• v.24 no.6
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• pp.732-739
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• 2014

We describe the development of a polymerase chain reaction method for the rapid, precise, and specific detection of the Xanthomonas oryzae pv. oryzae (Xoo) K3a race, the bacterial blight pathogen of rice. The specific primer set was designed to amplify a genomic locus derived from an amplified fragment length polymorphism specific for the K3a race. The 1,024 bp amplicon was generated from the DNA of 13 isolates of Xoo K3a races out of 119 isolates of other races, pathovars, and Xanthomonas species. The assay does not require isolated bacterial cells or DNA extraction. Moreover, the pathogen was quickly detected in rice leaf 2 days after inoculation with bacteria and at a distance of 8 cm from the rice leaf 5 days later. The results suggest that this PCR-based assay will be a useful and powerful tool for the detection and identification of the Xoo K3a race in rice plants as well as for early diagnosis of infection in paddy fields.

• Journal of Powder Materials
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• v.26 no.6
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• pp.528-538
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• 2019

The transition from "More-of-Less" markets (economies of scale) to "Less-of-More" markets (economies of scope) is supported by advances of disruptive manufacturing and reconfigurable-supply-chain management technologies. With the prevalence of cyber-physical manufacturing systems, additive manufacturing technology is of great impact on industry, the economy, and society. Traditionally, backbone structures are built via bottom-up manufacturing with either pre-fabricated building blocks such as bricks or with layer-by-layer concrete casting such as climbing form-work casting. In both cases, the design selection is limited by form-work design and cost. Accordingly, the tool-less building of architecture with high design freedom is attractive. In the present study, we review the technological trends of additive manufacturing for construction-scale additive manufacturing in particular. The rapid tooling of patterns or molds and rapid manufacturing of construction parts or whole structures is extensively explored through uncertainties from technology. The future regulation still has drawbacks in the adoption of additive manufacturing in construction industries.

• Transactions of the Korean Society of Automotive Engineers
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• v.11 no.1
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• pp.160-165
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• 2003

The design and implementation of an engine control system has become an important area in developing a new car, but the implementation of an engine control system is becoming a tedious and time-consuming work as the level of complexity increases. In order to shorten the development cycle of the control system, rapid control prototyping (RCP) technique deserves developers' attention. A new RCP platform has been developed for an automotive engine control application. This prototyping system strictly adheres to the layered architecture of the final production ECU, and separates the automatically generated part of software, or the application area, from the hand coded area, which generally carefully designed and tested because of the hardware dependency and the efficiency of microcontroller. The $Matlab{\circledR}$ tool-chain of Mathworks Inc. has been selected as a base environment in this study. A newly developed Engine Control Toolbox of Real-Time $Workshop{\circledR}$ converts a graphically represented control algorithm into optimized application codes and links them with other parts of the software to generate executable code for the target processor.

• Journal of Microbiology and Biotechnology
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• v.27 no.5
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• pp.909-915
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• 2017

Bifidobacterium bifidum BGN4 (BGN4) has many proven beneficial effects, including antiallergy and anticancer properties. It has been commercialized and used in several probiotic products, and thus strain-specific identification of this strain is very valuable for further strain-dependent physiological study. For this purpose, we developed novel multiplex polymerase chain reaction (PCR) primer sets for strain-specific detection of BGN4 in commercial products and fecal samples of animal models. The primer set was tested on seven strains of B. bifidum and 75 strains of the other Bifidobacterium species. The BGN4-specific regions were derived using megaBLAST against genome sequences of various B. bifidum databases and four sets of primers were designed. As a result, only BGN4 produced four PCR products simultaneously whereas the other strains did not. The PCR detection limit using BGN4-specific primer sets was $2.8{\times}10^1CFU/ml$ of BGN4. Those primer sets also detected and identified BGN4 in the probiotic products containing BNG4 and fecal samples from a BGN4-fed animal model with high specificity. Our results indicate that the PCR assay from this study is an efficient tool for the simple, rapid, and reliable identification of BGN4, for which probiotic strains are known.

• Proceedings of the SCSK Conference
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• 2003.09a
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• pp.85-96
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• 2003

Normal human skin, constantly challenged by environmental micro-organisms, has an innate ability to fight invading microbes through antimicrobial peptides. These peptides, described in both plant and animal kingdoms are able to inactivate a broad spectrum of micro-organisms. Mammalian defensins constitute one of the most common antimicrobial peptide family. Among the three human beta-defensins hBD1, hBD2 and hBD3 produced in epithelia, only hBD2 and hBD3 are inducible and additionally have been described as expressed by differentiated keratinocytes at site of inflammation and infection. The aims of these studies were to define a cell culture model in which the basal production of hBD could be detected and up-regulated in order to enhance skin auto-protection against micro-organisms. A specific Polymerase Chain Reaction method have been developed for hBD2 and hBD3 mRNA detection in non-differentiated monolayer keratinocytes cell culture. We have been able to demonstrate that in vitro, hBD2 and hBD3 expression in normal human keratinocytes could be detected and enhanced by TNF-alpha and IFN-gamma, in hypercalcic culture conditions. This research opened the possibility of the development of cosmetic active compounds, able to induce the expression of skin natural antibiotic peptides responsible about microflora ecology of the skin.

• Communications for Statistical Applications and Methods
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• v.25 no.2
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• pp.131-157
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• 2018

We propose a robust event date model to estimate the date of a target event by a combination of individual dates obtained from archaeological artifacts assumed to be contemporaneous. These dates are affected by errors of different types: laboratory and calibration curve errors, irreducible errors related to contaminations, and taphonomic disturbances, hence the possible presence of outliers. Modeling based on a hierarchical Bayesian statistical approach provides a simple way to automatically penalize outlying data without having to remove them from the dataset. Prior information on individual irreducible errors is introduced using a uniform shrinkage density with minimal assumptions about Bayesian parameters. We show that the event date model is more robust than models implemented in BCal or OxCal, although it generally yields less precise credibility intervals. The model is extended in the case of stratigraphic sequences that involve several events with temporal order constraints (relative dating), or with duration, hiatus constraints. Calculations are based on Markov chain Monte Carlo (MCMC) numerical techniques and can be performed using ChronoModel software which is freeware, open source and cross-platform. Features of the software are presented in Vibet et al. (ChronoModel v1.5 user's manual, 2016). We finally compare our prior on event dates implemented in the ChronoModel with the prior in BCal and OxCal which involves supplementary parameters defined as boundaries to phases or sequences.

• Korean Journal of Agricultural Science
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• v.42 no.3
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• pp.269-276
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• 2015

Unmanned Aerial Vehicle (UAV) has several advantages over conventional remote sensing techniques. They can acquire high-resolution images quickly and repeatedly. And with a comparatively lower flight altitude, they can obtain good quality images even in cloudy weather. In this paper, we discussed the state-of-the-art of the domestic and international use of UAV in agricultural sector as well as assessed its utilization and applicability for agricultural environment in Korea. Association of robotic, computer vision and geomatic technologies have established a new paradigm of low-altitude aerial remote sensing that has now been receiving attention from researchers all over the world. In a field study, it has been found that use of UAV imagery in an agricultural subsidy program can reduce the farmers' complain and provide objective evidence. UAV high resolution photography can also be helpful in monitoring the disposal zone for animal carcasses. Due to its expeditiousness and accuracy, UAV imagery can be a very useful tool to evaluate the damage in case of an agricultural disaster for both parties insurance companies and the farmers. Also high spatial and temporal resolution in UAV system can increase the prediction accuracy which in turn help to maintain the agricultural supply and demand chain.

• Journal of Ocean Engineering and Technology
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• v.24 no.4
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• pp.86-93
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• 2010

RFID has been used as an identification tool that substitutes for bar codes. Its areas of application are increasing due to its suitability in ubiquitous environment. In this paper, first, we review various aspects of RFID, including standards, characteristics, and relationships with wireless sensor networks. Then, we review the technical aspects of RFID and its areas of application, e.g., supply chain management (SCM) and manufacturing since 2005. SCM, in which RFIDs can shorten lead time and ensure the quality of products, is one of the most active application areas. Manufacturing is a relatively new area for RFID applications, but it is believed that it can enhance the productivity and the reliability of the products. Also, the authors suggest research issues and limitations of RFID, as well as possible application areas related to marine engineering.

• Proceedings of the Korean Operations and Management Science Society Conference
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• 2003.05a
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• pp.217-224
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• 2003

ISO 14649 is a new interface (or language) standard for the CAD-CAM -CNC chain, currently under establishment by ISO TC184 SCI and SC4 . Upon completion, it will replace ISO 6983, so called M amp; G codes used for CNC since 1950' s. As the new language is being established, a new CNC controller called STEP-CNC (STEP­compliant CNC), capable of carrying out various intelligent tasks using the new language as an input, receives worldwide attention. In this paper, we present a distributed architecture for STEP-NC system based on the generic paradigm of STEP-NC. The STEP-NC system is consisted of 3 sub-systems: 1) CGS (Code Generation System), 2) CES (Code Edit System), and 3) ACS (Autonomous Control System). Also presented in this paper is algorithm for delta volume decomposition, a crucial algorithm for developing CGS. First method is based on the cutting tool and the second method is based on the turning features commonly used in the shop floor. An illustrative example is given to compare the two methods, and to illustrate usage scenario of the delta volume in the turning STEP- NC system under development.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2003.06a
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• pp.1042-1045
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• 2003

In this paper, we present a new method for the monitoring of Electric Control Unit's(ECU) self-diagnostic and the sensor signals of vehicle through Web. In order to measure the ECU's self-diagnostic and sensor signals, the interfaced circuit is designed to communicate ECU and terminal according to the ISO, SAE regulation of communication protocol standard. Microprocessor 80C196KC is used for communicating ECU's self-diagnostic signals and the results are sent to the Embedded Linux System(ELS) through RF module. ELS is developed by SA1110, RF module, Embedded Linux. All commands related in ECU communication are executed through Web. The CGI program composed in web server is executed by user and will return sensor signals from ECU Software on Embedded Linux system is developed to monitor the ECU's sensor signals using the arm compiler tool chain in which RS232 port is programmed by half duplex method. The possibility for remote measurement of ECU sensor signal through Web is verified through the developed systems and algorithms.