• Journal of Microbiology and Biotechnology
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• v.15 no.6
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• pp.1397-1401
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• 2005

Bone morphogenetic protein-4 (BMP-4) is a signaling homodimeric molecule that acts as a morphogen to influence cell fate in a concentration-dependent manner. The limited supply of a pure preparation of BMP-4, due to very low level of their expression in vivo, makes it difficult not only to study the biological activities of BMPs, but also to use them as a clinical tool. For a large-scale production of BMP-4, human BMP-4 cDNA was expressed in Chinese hamster ovary (CHO) cells by a recently development vector system, which confers position-independent stable expression of the foreign genes. The CHO cell line expressing recombinant human BMP-4 (rhBMP-4) at the level of $7\;{\mu}g/ml$ could be obtained after stepwise selection with methotrexate. This level of expression is about 70 times higher than those previously reported. The partially processed form of BMP-4 as well as mature form could be detected, when the aliquots of culture media were analyzed by Western blot. The glycosylation pattern and biological activity of the rhBMP-4 were determined by glycosidase treatment and the induction rate of alkaline phosphatase in mouse osteoblastic cells.

• Journal of Microbiology and Biotechnology
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• v.17 no.3
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• pp.490-495
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• 2007

Lactic acid bacteria (LAB) are beneficial for the gastrointestinal tract and reinforce immunity in human health. Recently, many functional products using the lactic acid bacteria have been developed. Among these LAB, Lactobacillus acidophilus, Lactobacillus rhamnosus, Bifidobacterium longum, and Bifidobacterium bifidum are frequently used for probiotic products. In order to monitor these LAB in commercial probiotic products, a multiplex PCR method was developed. We designed four species-specific primer pairs for multiplex PCR from the 16S rRNA, 16S-23S rRNA intergenic spacer region, and 23S rRNA genes in Lactobacillus acidophilus, Lactobacillus rhamnosus, Bifidobacterium longum, and Bifidobacterium bifidum. Using these primer pairs, 4 different LAB were detected with high specificity in functional foods. We suggest that the multiplex PCR method developed in this study would be an efficient tool for simple, rapid, and reliable identification of LAB used as probiotic strains.

• Journal of Korea Foundry Society
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• v.26 no.2
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• pp.85-91
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• 2006

Inclusions in Ti investment castings are generally known to have detrimental effects on the performance of the castings. However, actual inclusions are infrequent and hard to be located. As a result, it is extremely difficult to obtain sufficient amount of fatigue test specimens of titanium investment castings having inclusions in the gage section. Thus, in-depth research of the adverse influence of inclusions is also hindered. To address this problem, a new casting methodology of specimens containing hard alpha inclusions was developed in this study. To guarantee successful introduction of an inclusion and casting, a carefully designed mold with 8 legs and a special tool were employed. After solidification, castings were cut, and X-ray radiography determined that the inclusions were successfully incorporated into the castings. The castings were further prepared to obtain multiple test specimens and they were fatigue-tested consecutively. Fractography analysis confirmed that fatigue cracks initiated at the hard alpha inclusion. In a nonlinear regression model, the fatigue life can be modeled as an exponential function with a negative exponent of the cross-sectional area of an inclusion. The fatigue life of Ti specimens containing inclusions is inversely proportional to the cross-sectional area of an inclusion.

• Journal of Biomedical Engineering Research
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• v.41 no.4
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• pp.154-164
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• 2020

Various simulator systems for surgery training have been developed and recently become more widely utilized with technology advancement and change in medical education adopting actively simulation-based training. The authors have developed tissue-instrument interaction modeling and graphical simulation algorithms for an arthroscopic surgery training simulator system. In this paper, we propose algorithms for basic surgical techniques, such as cutting, shaving, drilling, grasping, suturing and knot tying for rotator cuff surgery. The proposed method constructs a virtual 3-dimensional model from actual patient data and implements a real-time deformation of the surgical object model through interaction between ten types of arthroscopic surgical tools and a surgical object model. The implementation is based on the Simulation Open Framework Architecture (SOFA, Inria Foundation, France) and custom algorithms were implemented as pulg-in codes. Qualitative review of the developed results by physicians showed both feasibility and limitations of the system for actual use in surgery training.

• Journal of the Korean Solar Energy Society
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• v.23 no.1
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• pp.57-67
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• 2003

This study aims to presents Evaluation Method of Green Remodeling that analyze the value of environment through expense, using the method of life cycle cost and life cycle assessment simultaneously. The results of this study are summarized as follows. Evaluation Model developed in this study can convert economical value of environment into cost by integrating. In addition, the model can apply as a useful tool to estimation of economical design alternative as well as quantification of environmental loads and costs. Evaluation Model presented In this study observe energy consumption and the environmental load emission with qualification, it can forecast effect of environmental cost that cost estimation is expected to be added to energy cost rate by being possible. Synthetically, when Estimation Model and computer program that developed in this study is applies to the construction industry; reasonable management of environmental load is convenient at each step of Green Remodeling. In addition, at preliminary design phase, practical use may be possible by reasonable yardstick about various alternatives and improvement of design alternatives likewise by grasping environmental effect.

• Journal of the Korean housing association
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• v.20 no.5
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• pp.93-102
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• 2009

Housing is a cultural tool which enables one to see through a certain generation, and the types of housing in a specific cultural area are both physical and spiritual results formed by life activities. Therefore, housing, the center area for the human life, is a bowl, putting various meanings of life into the complicated environment. Its formal and spacial variety is a result of interaction in human life. This study analyzes fundamental problems and characteristics of asian traditional housing by comparing those in city of Seoul and Shang-hai. From the results of this study, many differences have been found in traditional housing in Seoul and Shanghai despite of high similarity m culture and ideology. This results from a same Confucian culture that accepts the feng shui theory which has evolved through different environments. Therefore, this study aims to understand housing cultures in two cities by analysing the attributes of housing data from selected traditional housing types in Seoul and Shanghai. Also, issues of fundamental characteristics of the two cities through the development of residential environment are studied to provide important data for the future development of housing environment of both cities.

• Transactions of the Korean Society of Machine Tool Engineers
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• v.13 no.6
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• pp.94-101
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• 2004

The purpose of this raper is to assess the benefits of frequency domain fatigue analysis and compare it with more conventional time domain techniques. The multi-body dynamic analysis, FE analysis and fatigue life prediction technique are applied for the frequency domain fatigue analysis. To obtain the dynamic load history used in the frequency domain fatigue analysis, the computer simulations running over typical road Profiles are carried out by utilizing vehicle dynamic model. The fatigue life estimation for the rear suspension system of small-sized passenger car is performed by using resonance durability analysis technique, and the estimation results are compared with the conventional quasi-static durability analysis results. For the pothole simulation, the percent changes, of the fatigue life between the two durability analysis techniques don't exceed 10%. But for the Belgian road simulation because of the resonance effect, the fatigue life using the resonance durability analysis technique are much smaller estimated than the quasi-static durability analysis results.

• Korean Journal of Adult Nursing
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• v.18 no.3
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• pp.395-404
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• 2006

Purpose: This study investigated the effect of a structured group intervention on knowledge about lung cancer, self efficacy and quality of life for family caregivers of patients with lung cancer using a nonequivalent control groupquasi-experimental design. Methods: Subjects were 11 family caregivers for both the control and the experimental group. The experimental group participated in once a week for 2-hour session for 4 weeks. Four topics of educational program were lung cancer and treatment, side effects of treatments, symptoms management, and health management. Every session consisted of lecture, sharing experiences, and meditating time. Quality of life was measured using Jang(1996)'s tool. The tools for knowledge and self-efficacy were developed by the authors. Results: After the intervention, the experimental group showed higher self-efficacy in caring for the patients than did the controls. However, there were no significant differences in knowledge about lung cancer and quality of life between the two groups. Conclusion: Findings indicate that the group intervention would be effective for family caregivers of lung cancer patients.

• Transactions of Materials Processing
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• v.26 no.3
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• pp.137-143
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• 2017

In this study, a wear test method was proposed to predict the wear life of the CrN layer coated on the surface of the press tools for manufacturing the auto-parts with ultra high strength steel (UHSS) with a tensile strength of 1.5 GPa. The pin-on-disc type wear test was carried out to confirm the feasibility and the reproducibility of the wear amount according to the test conditions such as the normal force, the sliding velocity, and the sliding speed. The test conditions were obtained from the finite element stamping analysis and the wear simulation. With the wear amount from the wear test, a prediction model of the wear depth in the CrN coating layer was proposed according to the test conditions with the design of experiments such as Taguchi method and the response surface method. The derived prediction model was then compared to the result of the Archard wear model, fully describing that the proposed model can effectively predict the wear life of the press tools for the auto-parts with UHSS.

• Proceedings of the KSR Conference
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• 2009.05a
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• pp.365-370
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• 2009

LCA is a tool to support for making decisions by offering information of environmental aspects of products or services. It can be used to make decisions to consumers and NGOs as well as government and enterprises. LCA evaluates the environmental aspects throughout the entire life cycle of a product. Therefore it can quantify and assess environmental impacts from raw material acquisition, manufacturing, distribution, use and disposal to end of life and recycling. The demands of the recycling rate increase and the use of suitable materials for RoHS, REACH, WEEE, ELV which are linked trade with environmental regulation have increased the worldwide. Global warming is the critical challenge of the world facing. And under post-Kyoto protocol each country has to prepare for target reduction, so it became essential to save energy and resources. In addition that, the carbon mark has been run as the way of showing example of CO2 reduction in domestic and it will be extended gradually. And also through the introduction of Eco-label, environmentally-friendly product will be promoted. When those systems are operated, global warming gases (i.e. CO2) can be calculated throughout the entire life of the products by LCA. And the environmental impacts such as harmful material emission in the process of manufacturing, energy consumption, distribution and so on, can also be assessed. Therefore, The basic concepts of LCA technique and various cases and the practical application in the future will be review in this study.