• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2001년도 추계학술발표대회
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• pp.177-180
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• 2001

Tobacco plants were transformed by A. tumefaciens harboring human ferritin gene and they were subjected to investigate for the expression of transformed gene as well as heavy metal accumulation. Seed from self-fertilized transgenic plants was germinated on media containing toxic level of Cd, Cu, Zn, Fe, Mn and scored for tolerance to this heavy metals. There is difference in growth rate between transgenic and control plants, especially Cd, Cu. And transgenic plants accumulated more heavy metals than control plants.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 1993년도 한국자동제어학술회의논문집(국내학술편); Seoul National University, Seoul; 20-22 Oct. 1993
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• pp.959-964
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• 1993

We developed fault diagnosis fuzzy expert system for ACS(Advanced Control System). ACS is a DCS(Distributed Control System) with advanced control algorithm fault tolerance capabilities, fault diagnosis functions, and so on. Fuzzy expert system developed for an ACS in this paper gives an operator alarm signal depending on the state of process value and manipulated value, and the cause of alarm in real time. Simple experiment result with several rules for the-fault-diagnosis of drum level loop in Seoul-Power-Plant.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 추계학술발표대회 및 bio-venture fair
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• pp.590-592
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• 2000

The indole tolerance level of Pseudomonas savastanoi BCNU 106 was as high as 300 mg/ml when toluene or p-xylene was added to the medium to 20% by volume. Pseudomonas savastanoi BCNU 106 grown in a two-phase system containing the various concentrations of indole and solvents produced indigo and indirubin. The optimal condition in the production of indigo and indirubin was also studied.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2001년도 춘계 학술대회지
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• pp.246-247
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• 2001

• Journal of Plant Biotechnology
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• 제5권3호
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• pp.173-179
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• 2003

The combination of a radiation technique with an in vitro culture system was appiled to develop submergence tolerant rice. The 3,000 $M_3$ lines with an average 80 percent of fertile grain were utilized for the selection of submergence tolerance. Salt tolerant lines were selected based on high plant height, root length and root number after submergence in plastic pots. Of the lines tested, the tolerant line (403-6) showed a dramatic difference in morphological traits under submergence compared to its original variety (Dongjinbyeo). It was suggested that genetic variations between the original variety and $M_3$-403-6 did exist. The levels of $\alpha$-amylase and alcohol dehydrogenase activities were significantly increased in the mutant line compared to its original variety. The mutant with greater tolerance showed less electrolyte leakage indicating a greater membrane integrity and better survival. Also, this line was much more resistant to a salt stress of $1.25\%$ than the original variety. The proline level of the line was significantly (p<0.01> higher than that of the original variety. The relationships between the inhibition of growth caused by stress and the physiological changes in the plant cell were discussed.

• The Korean Journal of Physiology and Pharmacology
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• 제2권6호
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• pp.671-676
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• 1998

In the present study, we first examined whether the changes in the DNA binding activities of the transcription factors, cAMP response element binding protein (CREB) and activator protein-1 (AP-1) mediate the long-term effects of morphine in differentiated SH-SY5Y human neuroblastoma cells. The increases in CREB and AP-1 DNA binding activities were time-dependent up to 6 days of morphine treatment (1, 4, and 6 days). However, the significant reduction in the DNA binding activities of CREB and AP-1 was observed after 10 days of chronic morphine $(10\;{\mu}M)$ administration. Secondly, we examined whether the changes of CREB and AP-1 DNA binding activities could be modulated by dopamine and haloperidol. Dopamine cotreatment moderately increased the levels of the CREB and AP-1 DNA binding activities induced by 10 days of chronic morphine treatment, and haloperidol cotreatment also resulted in a moderate increase of the CREB and AP-1 DNA binding activities. However, dopamine or haloperidol only treatment showed a significant increase or decrease of the CREB and AP-1 DNA binding activities, respectively. In the case of acute morphine treatment, the CREB and AP-1 DNA binding activities were shown to decrease in a time-dependent manner (30, 60, 90, and 120 min). Taken these together, in differentiated SH-SY5Y cells, morphine tolerance seems to involve simultaneous changes of the CREB and AP-1 DNA binding activities. Our data also suggest the possible involvement of haloperidol in prevention or reversal of morphine tolerance at the transcriptional level.

• Journal of Plant Biotechnology
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• 제42권4호
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• pp.409-413
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• 2015

본 연구는 토양에 오염된 중금속을 제거하기 위한 식물정화공정에 사용할 식물체를 개발하기 위해 $TgMTP_1$ 유전자를 CaMV35S 상시 발현 할 수 있도록 Ti-plasmid 벡터를 구축하여 형질전환식물을 육성하였다. 유전자가 도입된 형질전환 애기장대에서 TgMTP유전자를 과발현하는 호모 TG-116 (T3 generation) 계통을 육성하여 그 특성을 조사하였다. 호모계통으로 육성한 TG-116 계통은 callus 및 식물체에서 중금속에 대한 저항성을 보였다. 특히 RT-PCR 및 Western 분석에서 유전자의 발현은 잎에서 높게 나타났으며, Ni 흡수 및 축적이 많이 일어났다. 따라서 MTP1 유전자가 발현되어 액포에 중금속을 축적하는 실험결과를 활용한다면 식물정화공정에 사용할 수 있는 다양한 유전자원으로 기대할 수 있다.

• 방송공학회논문지
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• 제18권1호
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• pp.1-9
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• 2013

모델 기반 카메라 추적에서 추적을 위해 사용되는 3차원 객체 모델의 정확도는 매우 중요하다. 하지만 3차원 객체의 실측 모델링은 일반적으로 정교한 작업을 요구할 뿐만 아니라, 오차 없이 모델링하기가 매우 어렵다. 반면에 오차를 포함하고 있는 3차원 객체 모델을 이용하더라도 모델링 오차에 의해서 계산되는 추적 오차와 실제 사용자의 육안으로 느끼는 추적 오차는 다를 수 있다. 이는 처리비용이 높은 정밀한 모델링 과정을 요구하지 않더라도 사용자가 느끼는 오차 허용 범위 내에서 추적을 위한 객체 모델링을 효과적으로 수행할 수 있기에 중요한 측면이 된다. 따라서 본 논문에서는 모델 기반 카메라 추적에서 모델링 오차에 따른 실제 정합 오차와 사용자의 육안으로 인지되는 정합 오차를 사용자 평가를 통해 비교 분석하고, 3차원 객체 모델링의 허용 오차 범위에 대해 논의한다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.175-175
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• 2017

To understand molecular mechanisms underlying adaptation of plant cells to saline stress and stress memory, we developed Arabidopsis callus suspension-cultured cells adapted to high salt. Adapted cells to high salt exhibited enhanced tolerance compared to control cells. Moreover, the salt tolerance of adapted cells was stably maintained even after the stress is relieved, indicating that the acquired salt tolerance of adapted cells was memorized. In order to characterize metabolic responses of plant cells during adaptation to high salt stress as well as stress memory, we compared metabolic profiles of salt-adapted and stress-memorized cells with control cells by using NMR spectroscopy. A principle component analysis showed clear metabolic discrimination among control, salt-adapted and stress-memorized cells. Compared with control cells, metabolites related to shikimate metabolism such as tyrosine, and flavonol glycosides, which are related to protective mechanism of plant against stresses were largely up-regulated in adapted cell lines. Moreover, coniferin, a precursor of lignin, was more abundant in salt-adapted cells than control cells. Cell morphology analysis using transmission electron microscopy indicated that cell wall thickness of salt-adapted cells was significantly induced compared to control cells. Consistently, salt adapted cells contained more lignin in their cell walls compared to control cells. The results provide new insight into mechanisms of plant adaptation to saline stress as well as stress memory in metabolic level.

• 천문학회지
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• 제51권2호
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• pp.27-36
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• 2018

In a solar coronagraph, the most important component is an occulter to block the direct light from the disk of the sun. Because the intensity of the solar outer corona is $10^{-6}$ to $10^{-10}$ times of that of the solar disk ($I_{\odot}$), it is necessary to minimize scattering at the optical elements and diffraction at the occulter. Using a Fourier optic simulation and a stray light test, we investigated the performance of a compact coronagraph that uses an external truncated-cone occulter without an internal occulter and Lyot stop. In the simulation, the diffracted light was minimized to the order of $7.6{\times}10^{-10}I_{\odot}$ when the cone angle ${\theta}_c$ was about $0.39^{\circ}$. The performance of the cone occulter was then tested by experiment. The level of the diffracted light reached the order of $6{\times}10^{-9}I_{\odot}$ at ${\theta}_c=0.40^{\circ}$. This is sufficient to observe the outer corona without additional optical elements such as a Lyot stop or inner occulter. We also found the manufacturing tolerance of the cone angle to be $0.05^{\circ}$, the lateral alignment tolerance was $45{\mu}m$, and the angular alignment tolerance was $0.043^{\circ}$. Our results suggest that the physical size of coronagraphs can be shortened significantly by using a cone occulter.