• The Plant Pathology Journal
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• v.38 no.3
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• pp.203-211
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• 2022

Bacterial wilt, which is a major soil-borne disease with widespread occurrence, poses a severe danger in the field of tobacco production. However, there is very limited knowledge on bacterial wilt-induced microecological changes in the tobacco root system and on the interaction between Ralstonia solanacearum and fungal communities in the rhizosphere soil. Thus, in this study, changes in fungal communities in the rhizosphere soil of tobaccos with bacterial wilt were studied by 18S rRNA gene sequencing. The community composition of fungi in bacterial wilt-infected soil and healthy soil in two tobacco areas (Gengma and Boshang, Lincang City, Yunnan Province, China) was studied through the paired comparison method in July 2019. The results showed that there were significant differences in fungal community composition between the rhizosphere soil of diseased plants and healthy plants. The changes in the composition and diversity of fungal communities in the rhizosphere soil of tobaccos are vital characteristics of tobaccos with bacterial wilt, and the imbalance in the rhizosphere microecosystem of tobacco plants may further aggravate the disease.

• Journal of Microbiology and Biotechnology
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• v.34 no.9
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• pp.1890-1897
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• 2024

Fermentation has been identified as an effective strategy to alter the chemical makeup of tobacco, thereby enhancing its quality. The deliberate introduction of microorganisms can hasten the fermentation process. In this research, microbial consortia harvested from the tobacco surface were utilized to enhance the tobacco quality. This enhancement also elevated several sensory attributes of HnB cigarettes, such as aroma richness, moisture, strength, and reduced irritation, achieving a sensory quality rating of 84.5. This marks a notable improvement compared to the 82 rating of the original, unfermented cigarettes. Untargeted metabolomics analysis revealed a decrease in total polyphenols and unsaturated fatty acids, while the levels of polyacids, alcohols, ketones, furans, and other compounds increased in the fermented tobacco. Additionally, KEGG pathway enrichment analysis indicated that the enhancement in tobacco quality through microbial consortia fermentation is linked to various biological pathways, with pathways related to fatty acid and amino acid degradation playing pivotal roles. The findings of this study will serve as a reference for the commercial production of HnB cigarettes, and the elucidated mechanism offers a theoretical basis for exploring microbial fermentation as a means to improve tobacco quality.

• Journal of Information Processing Systems
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• v.18 no.4
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• pp.480-488
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• 2022

Images of tobacco retail licenses have complex unstructured characteristics, which is an urgent technical problem in the robot process automation of tobacco marketing. In this paper, a novel recognition approach using a double attention mechanism is presented to realize the automatic recognition and information extraction from such images. First, we utilized a DenseNet network to extract the license information from the input tobacco retail license data. Second, bi-directional long short-term memory was used for coding and decoding using a continuous decoder integrating dual attention to realize the recognition and information extraction of tobacco retail license images without segmentation. Finally, several performance experiments were conducted using a largescale dataset of tobacco retail licenses. The experimental results show that the proposed approach achieves a correction accuracy of 98.36% on the ZY-LQ dataset, outperforming most existing methods.

• Journal of Information Processing Systems
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• v.18 no.3
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• pp.311-318
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• 2022

Tobacco sales enterprises often need to summarize and verify the daily sales bills, which may consume substantial manpower, and manual verification is prone to occasional errors. The use of artificial intelligence technology to realize the automatic identification and verification of such bills offers important practical significance. This study presents a novel multi-branch residual network for tobacco sales bills to improve the efficiency and accuracy of tobacco sales. First, geometric correction and edge alignment were performed on the input sales bill image. Second, the multi-branch residual network recognition model is established and trained using the preprocessed data. The comparative experimental results demonstrated that the correct recognition rate of the proposed method reached 98.84% on the China Tobacco Bill Image dataset, which is superior to that of most existing recognition methods.

• Proceedings of the Korean Society of Tobacco Science Conference
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• 1997.10a
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• pp.13-29
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• 1997

• The Journal of the Korea Contents Association
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• v.21 no.6
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• pp.165-179
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• 2021

This study investigated that what strategies tobacco companies have taken for marketing tobacco and tobacco companies on news media, by reviewing the previous research analyzing the tobacco companies' documents and the news coverage of tobacco. The tobacco document studies showed that tobacco companies have created close relationships with news media, a quid pro quo, by providing advertising and diverse forms of sponsorships and obtaining favored treatment on news coverage and have built networks with journalists by supporting journalist organizations. They have also supported thinktank groups and pro-smoking groups so as to represent and advocate their perspectives and assertions on news media and have utilized sponsorship of art and culture as pseuo-event for publicizing tobacco and tobacco companies on news media. Futhermore, news coverage of tobacco showed that news media tended to deal with tobacco issues lukewarmly, without a clear position and perspective and deliver naturally tobacco industry's perspective and message that smoking is controvertial rather than totally undesirable and unacceptable. Also, the news coverages have often reflected tobacco industry's publicity strategies identified in tobacco industry documents. This study suggests how tobacco industry access and utilize news media to obtain favorable news coverage, and thus it provides a rational of why tobacco industry's publicity activities have to be regulated.

• Journal of the Korean Society of Tobacco Science
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• v.29 no.1
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• pp.41-51
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• 2007

This study was implemented to examine the effect of tobacco on operation of a retail store and propose a relevant direction to development of a retail store. In terms of an examination method, the questionnaire was conducted in respect of supermarkets and convenience stores(CVS) around the nation. As a result of analysis based on such questionnaire, although there was a little difference in between a supermarket and a convenience store, a similar result could be found in general. In other words, retailers selling tobacco regarded the tobacco as a medium to solicit customers. Since the significance of tobacco in terms of total sales volume was so high, without the tobacco, they might have suspend the business. Consequently, the effect of tobacco was enormous. In addition, under the premise that many regular customers should be acquired to develop such retail stores, it was found that it would be necessary to increase $15{\sim}20%$ in terms of a margin in tobacco, preserve the profit ratio by the government, make efforts to enhance a quality and design by a tobacco company, actively recommend a particular brand, and actively display a tobacco publicity booklet, etc. Moreover, the proper number of tobacco stores in comparison with habitual smokers was one per 200 persons. In respect of the most unreasonable tobacco policy, a minor-related system and a policy of increasing a tobacco price were named. Thus, under the premise that a medium such as tobacco is highly important with respect to an operation of retail store, it is necessary to pay a more careful attention to an improvement of a reasonable and equitable system to further develop a retail store.

• The Plant Pathology Journal
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• v.38 no.6
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• pp.583-592
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• 2022

Root-knot nematode disease is a widespread and catastrophic disease of tobacco. However, little is known about the relationship between rhizosphere bacterial community and root-knot nematode disease. This study used 16S rRNA gene sequencing and PICRUSt to assess bacterial community structure and function changes in rhizosphere soil from Meloidogyne incognita-infected tobacco plants. We studied the rhizosphere bacterial community structure of M. incognita-infected and uninfected tobacco plants through a paired comparison design in two regions of tobacco planting area, Yuxi and Jiuxiang of Yunnan Province, southwest China. According to the findings, M. incognita infection can alter the bacterial population in the soil. Uninfested soil has more operational taxonomic unit numbers and richness than infested soil. Principal Coordinate Analysis revealed clear separations between bacterial communities from infested and uninfested soil, indicating that different infection conditions resulted in significantly different bacterial community structures in soils. Firmicutes was prevalent in infested soil, but Chloroflexi and Acidobacteria were prevalent in uninfested soil. Sphingomonas, Streptomyces, and Bradyrhizobium were the dominant bacteria genera, and their abundance were higher in infested soil. By PICRUSt analysis, some metabolism-related functions and signal transduction functions of the rhizosphere bacterial community in the M. incognita infection-tobacco plants had a higher relative abundance than those uninfected. As a result, rhizosphere soils from tobacco plants infected with M. incognita showed considerable bacterial community structure and function alterations.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2000.11c
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• pp.569-577
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• 2000

The position of tobacco leaves is affluence to the quality. To evaluate its quality, sample leaves was collected according to the position of attachment. In Korea, the position was divided into four classes such as high, middle, low and inside positioned leaves. Until now, the grade of standard sample was determined by human expert from korea ginseng and tobacco company. Many research were done by the chemical and spectrum analysis using NIR and computer vision. The grade of tobacco leaves mainly classified into 5 grades according to the attached position and its chemical composition. In high and low positioned leaves shows a low level grade under grade 3. Generally, inside and medium positioned leaf has a high level grade. This is the basic research to develop a real time tobacco leaves grading system combined with portable NIR spectrum analysis system. However, this research just deals with position recognition and grading using the color machine vision. The RGB color information was converted to HSI image format and the sample was all investigated using the bundle of tobacco leaves. Quality grade and position recognition was performed through well known general error back propagation neural network. Finally, the relationship about attached leaf position and its grade was analyzed.

• Journal of Environmental Health Sciences
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• v.8 no.2
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• pp.33-46
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• 1982

This experiment was carried out for the purpose of reducing alkaloid in reconstituted tobacco sheet and effluent of reconstituted tobacco sheet manufacturing company by treating oxidizing agents such as ozone, sodium hypochlorite, perchloric acid and hydrogen peroxide to tobacco extract created from the manufacturing process of reconstituted tobacco sheet. The effect of alkaloid reduction in tobacco extract by the volume added, time of treatment and pH of oxidizing agents were as follows: 1. When the solid rate of tobacco extract stood at 10 percent, the content of alkaloid, total sugar, total nitrogen and chlorine was 1,600mg/l, 11,000mg/l, 3,200mg/l and 4,000mg/l, respectively. 2. The effect of alkaloid reduction through ozone treatment was in proportion to time of ozone treatment. Alkaloid showed a 31.2 percent reduction under 8 hours' ozone treatment and 0.23g ozone consumed to remove lmg alkaloid. 3. Alkaloid reduction through sodium hypochlorite treatment was influenced by quantity of chlorine in sodium hypochlorite solution. To remove lmg alkaloid, 36.3mg chlorine was used. Reduction of alkaloid was not affected by time of sodium hypochlorite treatment, while showed the best reaction under pH 5-7. 4. The effect of alkaloid reduction by perchloric acid was under the control of the volume added and time of treatment of perchloric acid. The volume of perchloric acid required to remove alkaloid was on the decrease as time of treatment was getting longer. lmg alkaloid was removed by 0.15g perchloric acid under 8 hours' perchloric acid treatment. 5. Alkaloid reduction reacted slowly to the volume added and time of treatment of hydrogen peroxide. Under 8 hours' hydrogen peroxide treatment, it showed maximum removal, registering 10 percent alkaloid reduction.