• 대한수의학회지
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• 제56권3호
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• pp.147-153
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• 2016

A total of 782 blood and 465 tissue samples from 1,039 wild animals and 127 dairy goats were collected from January 2011 to December 2013 in 10 provinces of South Korea and tested for the presence of brucellosis. The Rose Bengal test revealed that 8.0% (52/650) of the serum samples were seropositive, while 4.2% (33/782) of the serum samples were positive for Brucella antibodies by competitive enzyme-linked immunosorbent assay. Of the 650 sera examined, only 16 (2.5%) were positive by both serological tests. Direct polymerase chain reaction (PCR) assay using B4/B5 primers for Brucella abortus (BCSP31) revealed the prevalence of Brucella to be 26.5% (129/487) in blood samples and 21% (98/465) in tissue samples while, 16S rRNA PCR detected Brucella DNA in 6.8% (33/487) and 2.6% (12/465) in blood and tissue samples, respectively. Of PCR-positive samples, only 6.2% (30/487) of blood samples and 2.4% (11/465) of tissue samples were found to be positive by both BCSP31 and 16S rRNA PCRs. However, Brucella strains were isolated by blood culture from only two out of 487 blood samples (0.4%). This characterization and identification of pathogenic Brucella isolates is the first to clearly indicate that the organisms were Brucella abortus biovar 1.

• 방사선산업학회지
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• 제6권2호
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• pp.159-164
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• 2012

Bacterial cellulose (BC) is generated from citrus gel by Gluconacetobacter hansenii TL-2C. BC has good properties such as high-burst pressure, high-water contact and the ultrafine highly nanofibrous structure of mimic natural extracellular matrix (ECM) for tissue engineering. In this study, acrylic acid (AAc) was grafted onto BC surfaces under aqueous conditions using gamma-ray irradiation, and then immobilized gelatin onto AAc-g-BC. The characterization of scaffolds was performed by scanning electron microscopy (SEM), attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), toluidine blue O (TBO) assay. Morphology of gelatin and AAc incorporation onto BC nanofibers did not changed. Our study suggests that gelatin-immobilized BC nanofibers scaffold has a potentiality to fabricate 3D nanofibrous scaffolds for tissue engineering.

• 대한기계학회논문집A
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• 제40권10호
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• pp.851-856
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• 2016

최근 미세가공기술이 발전함에 따라 생체분해성 소재 기반의 미소바늘 혹은 미소바늘 주사기에 대한 연구가 각광을 받고 있다. 일반적으로 생체분해성 소재는 기계적 강도가 낮아 생체분해성 소재 기반의 미소바늘은 피부조직 관통 시 구부려지거나 파손되는 문제점을 가지고 있다. 본 연구에서는 기계적 강도가 향상된 생체적합성 소재를 새로이 합성하는 대신에 미소바늘 삽입 전 피부조직에 1축 혹은 등2축 초기변형률을 가해 미소바늘의 피부조직 관통력을 감소시키는 방법에 대해 살펴본다. 실험에서는 1축 혹은 등 2축 초기변형률을 가해 준 돼지 피부조직에 미소바늘을 삽입시키면서 힘-시간 데이터를 획득함으로써 초기변형률에 의한 미소바늘의 관통력 변화를 측정하였다. 본 연구를 통해 미소바늘의 피부조직 관통력은 피부조직에 초기변형률을 가해주는 방법으로 감소시킬 수 있음을 확인하였다.

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2002년도 추계학술대회
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• pp.56-58
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• 2002

Understanding the molecular pathogenesis of the multifaceted host-pathogen interaction is critical in the development of improved treatment and prevention, as well as elucidating how certain bacteria can circumvent host defenses, multiply in the host, and cause such extensive damage. Disease caused by infection with V. vulnificus is remarkable for the invasive nature of the infection, ensuing severe tissue damage, and rapidly fulminating course. The characterization of somatic as well as secreted products of V. vulnificus has yielded a large list of putative virulence attributes, whose known functions are easily imagined to explain the pathology of disease. These putative virulence factors include a carbohydrate capsule, lipopolysaccharide, a cytolysin/hemolysin, elastolytic metalloprotease, iron sequestering systems, lipase, and pili. However, only few among the putative virulence factors has been confirmed to be essential for virulence by the use of molecular Koch's postulates. This presentation describes molecular biological characterization of the virulence factors contributing to survival as well as to toxigenesis of V. vulnificus.

• 대한견주관절학회:학술대회논문집
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• 대한견주관절학회 2007년도 제15차 대한견주관절학회 및 제3차 Mayo Asia Elbow Club 합동 학술대회
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• pp.62-62
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• 2007

• 대한의용생체공학회:의공학회지
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• 제7권2호
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• pp.117-118
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• 1986

It is well known that nonlinear propagation characteristics of the wave in the tissue may give very useful information for the medical diagnoisis. In this paper, a new method to detect nonlinear propagation characteristics of the internal vibration in the tissue for the low frequency mechanical vibration by using bispectral analysis is proposed. In the method, low frequency vibration of f0( = 100Hz) is applied on the surface of the object, and the waveform of the internal vibration x (t) is measured from Doppler frequency modulation of silmultaneously transmitted probing ultrasonic waves. Then, the bispectra of the signal x (t) at the frequencies (f0, f0) and (f0, 2f0) are calculated to estimate the nonlinear propagation characteristics as their magnitude ratio, w here since bispectrum is free from the gaussian additive noise we can get the value with high S/N. Basic experimental system is constructed by using 3.0 MHz probing ultrasonic waves and the several experiments are carried out for some phantoms. Results show the superiority of the proposed method to the conventional method using power spectrum and also its usefulness for the tissue characterization.

• 센서학회지
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• 제23권1호
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• pp.29-34
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• 2014

A dosimeter using tissue-equivalent scintillator by photon-counting method was developed and evaluated in its performance. The dosimeter is portable and can be operated by low power from lap-top computer. A data-acquisition software of the dosimeter system was developed by Labwindows/CVI based on Windows. The energy to channel ratio for energy calibration was 0.839 keV/ch. obtained from pulse height spectrum of $^{137}Cs$ and $^{60}Co$ gamma-ray. Using the dosimeter system, the absorbed dose of environmental radiation in Gyungju was 0.18 ${\mu}Sv/h$.

• 대한의용생체공학회:의공학회지
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• 제11권2호
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• pp.227-232
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• 1990

As a part of the study on ultrasonic tissue characterization, conventional ultrasonic imaging system is interfaced to the personal computer to acquire raw ultrasonic signal. One approach for tissue charaterization is performed using the attenuation map to the conventional images and the resulting attenuation map images are compared and inspected inside the region of interest from the viewpoint of pattern analysis. Currently, these methods are applied and modified to effectively find out the differences between the normal control and the patients with liver cirrhosis.

• Journal of Korean Neurosurgical Society
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• 제64권3호
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• pp.359-366
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• 2021

Neuromesodermal progenitors (NMPs) constitute a bipotent cell population that generates a wide variety of trunk cell and tissue types during embryonic development. Derivatives of NMPs include both mesodermal lineage cells such as muscles and vertebral bones, and neural lineage cells such as neural crests and central nervous system neurons. Such diverse lineage potential combined with a limited capacity for self-renewal, which persists during axial elongation, demonstrates that NMPs are a major source of trunk tissues. This review describes the identification and characterization of NMPs across multiple species. We also discuss key cellular and molecular steps for generating neural and mesodermal cells for building up the elongating trunk tissue.

• International Journal of Oral Biology
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• 제32권1호
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• pp.13-22
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• 2007

The stem cell research is emerging as a cutting edge topic for a new treatment for many chronic diseases. Recently, dental stem cell would be possible for regeneration of tooth itself as well as periodontal tissue. However, the study of the cell characterization is scarce. Therefore, we performed the genetic profiling and the characterization of mouse fetus/neonate derived dental tissue and cell to find the identification during dental development. We separated dental arch from mandibles of 14.5 d fetal mice and neonate 0 d under the stereoscope, and isolated dental cells primarily from the tissues. Then, we examined morphology and the gene expression profiles of the primary cells and dental tissues from fetus/neonate and adult with RT-PCR. Primary dental cells showed heterogeneous but the majority was shown as fibroblast-like morphology. The change of population doubling time levels (PDLs) showed that the primary dental cells have growth potential and could be expanded under our culture conditions without reduction of growth rate. Immunocytochemical and flow cytometric analyses were performed to characterize the primary dental cell populations from both of fetus (E14.5) and neonate. Alpha smooth muscle actin (${\alpha}-SMA$), vimentin, and von Willebrand factor showed strong expression, but desmin positive cells were not detected in the primary dental cells. Most of the markers were not uniformly expressed, but found in subsets of cells, indicating that the primary dental cell population is heterogeneous, and characteristics of the populations were changed during culture period. And mesenchymal stem cell markers were highly expressed. Gene expression profile showed Wnt family and its related signaling molecules, growth factors, transcription factors and tooth specific molecules were expressed both fetal and neonatal tissue. The tooth specific genes (enamelin, amelogenin, and DSPP) only expressed in neonate and adult stage. These expression patterns appeared same as primary fetal and neonatal cells. In this study we isolated primary cells from whole mandible of fetal and neonatal mice. And we investigated the characteristics of the primary cells and the profile of gene expressions, which are involved in epithelial-mesenchymal interactions during tooth development. Taken together, the primary dental cells in early passages or fetal and neonatal mandibles could be useful stem cell resources.