• 제목/요약/키워드: Time determination

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Bioluminescence 반응에 의한 ATP측정을 이용한 젖소 유방염 치료에 관한 연구 (Treatment test for bovine mastitis by the determination of ATP based on firefly bioluminescence)

  • 김태종;김종배;이승배;전용수
    • 대한수의학회지
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    • 제29권3호
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    • pp.393-405
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    • 1989
  • This study was carried out to treatment test for bovine mastitis by the determination of adenosine triphosphate (ATP) based on firefly bioluminescence. The results obtained are followed; 1. In the susceptibility test, cephalothin which looks the most effective were sensitive to Staphylococcus sp. (72.3%), Micrococcus sp. (84.2%), Streptococcus sp. (72.7%) and Gram positive bacilli (72.7%), Gram negative bacilli were sensitive to gentamicin (92.3%) and Yeast-like-fungi was the most sensitive to clotrimazole, and nystatin in order. 2. When the number of bacteria, such as Staphylococcus aureus, Candida tropicalis isolated from the mastitis milk were counted by conventional agar plating technique, and compared with the concentration of bacterial ATP, it gave a good linear relationship. The content of ATP per Staphylococcus aureus, cell was 3.1fM and Candida tropicalis showed the high level of A TP (90fM). 3. The ATP assay was applied to the determination of minimal inhibitory concentration (MIC) of various antibiotics. When Staphylococcus aureus was incubated in the presence of different concentration of tetracycline, erythromycin, kanamycin and streptomycin sulfate and the growth was monitored by the conventional agar plating technique and ATP assay, both methods shown the same results that they were 1mcg/ml, 2mcg/ml, 6.25mcg/ml and 8mcg/ml, respectively. 4. For the determination of susceptibility of sensitive and resistant Staphylococcus au reus isolated for the milk with mastitis to tetracycline, erythromycin kanamycin and streptomycin sulfate, the minimum time required for the test was determined by the assay of ATP every 30 minutes during incubation of 3 hours at $37^{\circ}C$. ATP concentration time curve calculated on both resistant and sensitive strains incubated 3 hours as the optimum time for the determination of susceptibilities of various antibiotics exemed. The ATP concentration of each test broth (antibiotic containing), expressed as a percentage of its own control brith (antibioticfree) indicated values of 30% to be indicative of each antibiotic sensitivity. Single time point ATP assay carried out on the various sensitive and resistant of Staphylococcus aureus to antibiotics examined after 3 hours at $37^{\circ}C$ correlated exactly with disc diffusion and MIC. 5. In the cure of intramammary treatment of bovine mastitis in lactating quarters, the cure rate of Staphylococcal mastitis showed to cephalexin (80%), cloxacillin and gentamicin (70%), ampicillin and oxytetracycline (60%), and Streptococcal mastitis showed to cephalexin (85%), penicillin (80%), cloxacillin and oxytetracycline (75%), and ampicillin (70%), but intramammary antimycotic drug (clotrimazol) were only a little effect about fungal mastitis.

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캘리포니아 테스트에 기초한 직접부하제어 프로그램의 적정 인센티브 산정 (Determination of Incentive Level of Direct Load Control Program Based on California lest)

  • 박종배;김민수;신중린;전영환
    • 에너지공학
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    • 제11권4호
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    • pp.342-349
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    • 2002
  • 본 논문은 캘리포니아 테스트를 이용하여 직접부하제어 프로그램의 적정 인센티브 수준 결정 방법을 제시한다. 대부분의 수요관리 프로그램의 경우 연간 혹은 수요관리 프로그램의 수명기간 동안 모든 고려 변수들이 상수로 주어지지만, 직접부하제어의 경우 연간 직접부하제어의 실행 회수 및 각 직접부하제어 실행 시간에 대하여 종속적인 값이 되기 때문에, 이러한 문제를 극복하고 직접부하제어의 경제성 평가에 효과적으로 적용하기 위해서 캘리포니아 테스트 방법론을 새롭게 정식화한다. 이 정식화를 사용하여 다양한 시나리오에 대한 사례연구를 수행하며, 현행 직접부하제어 프로그램의 인센티브 수준을 재검토하고 경쟁적 전력시장에서 직접부하제어 프로그램이 활성화될 수 있는 방안을 제안한다.

위치기반 서비스 고도화 기술 비교 분석 (Comparative analysis for advanced technologies of the location based service)

  • 심현보
    • 한국정보통신학회논문지
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    • 제16권4호
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    • pp.853-871
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    • 2012
  • 위치기반서비스는 넓은 지역을 커버하기 위해 인공위성이나 이동통신기지국을 이용하는 GPS와 건물 내부나 공원과 같이 한정된 공간(근거리)에서 Zigbee, RFID, CSS(Chirp Spread Spectrum), UWB, Bluetooth, Wi-Fi 장비 등을 이용하는 RTLS(Real Time Location System)로 나눌 수 있으며, 유선과 무선 인프라의 발전과 측위 기술의 고도화, 인터넷의 발전, 콘텐츠의 다양화 등을 통하여 기술적 발전을 거듭하고 있으며 향후 공통 핵심 기술 요소인 측위 기술, 콘텐츠 기술, 인터넷 및 이동통신기술 등을 중심으로 발전할 것으로 예상된다. 본 논문에서는 LBS의 발전방향에 대한 신기술과 표준화 기술을 조사 분석하여 관련 업계에 기여할 방향을 찾고자 한다.

Determination of Acrylamide in Foods by Solid Phase Microextraction-Gas Chromatography

  • Chen, Liangbi;Liu, Haizhu;Yu, Ping;Zhao, Jinyun;Chen, Xi
    • Food Science and Biotechnology
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    • 제18권4호
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    • pp.895-899
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    • 2009
  • A new approach for the determination of acrylamide (AM) in foods by solid phase microextraction-gas chromatography (SPME-GC) was established. AM was bromized and transformed to 2-bromoacrylamide (2-BAM). 2-BAM was then extracted by a commercial SPME fiber, $75-{\mu}m$ Car/PDMS fiber, for GC detection. The influence of extraction and desorption parameters such as extraction temperature and time, stirring rate, desorption temperature, and time were studied and optimized. The mass concentration was proportional to the peak area of 2-BPA from 1.0 to 8,000 ${\mu}g/L$. The detection limit of the SPME-GC for 2-BAM was found to be 0.1 ${\mu}g/L$, and the recoveries and relative standard deviations for different food samples were 74.5 to 102.0%, and 4.2 to 9.1%, respectively. The presented method was applied to the determination of AM in fried foods.

Orbit Determination of KOMPSAT-1 and Cryosat-2 Satellites Using Optical Wide-field Patrol Network (OWL-Net) Data with Batch Least Squares Filter

  • Lee, Eunji;Park, Sang-Young;Shin, Bumjoon;Cho, Sungki;Choi, Eun-Jung;Jo, Junghyun;Park, Jang-Hyun
    • Journal of Astronomy and Space Sciences
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    • 제34권1호
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    • pp.19-30
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    • 2017
  • The optical wide-field patrol network (OWL-Net) is a Korean optical surveillance system that tracks and monitors domestic satellites. In this study, a batch least squares algorithm was developed for optical measurements and verified by Monte Carlo simulation and covariance analysis. Potential error sources of OWL-Net, such as noise, bias, and clock errors, were analyzed. There is a linear relation between the estimation accuracy and the noise level, and the accuracy significantly depends on the declination bias. In addition, the time-tagging error significantly degrades the observation accuracy, while the time-synchronization offset corresponds to the orbital motion. The Cartesian state vector and measurement bias were determined using the OWL-Net tracking data of the KOMPSAT-1 and Cryosat-2 satellites. The comparison with known orbital information based on two-line elements (TLE) and the consolidated prediction format (CPF) shows that the orbit determination accuracy is similar to that of TLE. Furthermore, the precision and accuracy of OWL-Net observation data were determined to be tens of arcsec and sub-degree level, respectively.

Determination of Ultra Trace Levels of Copper in Whole Blood by Adsorptive Stripping Voltammetry

  • Attar, Tarik;Harek, Yahia;Larabi, Lahcen
    • 대한화학회지
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    • 제57권5호
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    • pp.568-573
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    • 2013
  • A selective and sensitive method for simultaneous determination of copper in blood by adsorptive differential pulse cathodic stripping voltammetry is presented. The procedure involves an adsorptive accumulation of Cu(II)-ETSC (4- ethyl-3-thiosemicarbazide) on a hanging mercury drop electrode, followed by a stripping voltammetry measurement of reduction current of adsorbed complex at about -715 mV. The optimum conditions for the analysis of copper (II) ion are : pH 10.3, concentration of 4-ethyl-3-thiosemicarbazide $3.25{\times}10^{-6}$ M and an accumulation potential of -100 mV. The peak current is proportional to the concentration of copper over the range 0.003-125 ng/mL with a detection limit of 0.001 ng/mL and an accumulation time of 60 s. Moreover, with the use of the proposed method, there is a considerable improvement in the detection limit, the linear dynamic range and the deposition time, compared with the methods of adsorptive stripping voltammetry for the determination of copper. The developed method was validated by analysis of whole blood certified reference materials.